Please review the QC test results indicated by icon below as well as clone information before placing your order.

pPyCAG-CreGR-IB

Expression clone of Cre-GR LBD fusion regulatable by dexamethasone. Blasticidin S resistance.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

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提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14288_A6Gqp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB14288_A6Gqa.seq
>RDB12117_14288_A6Gq_pAxCA_F1_G06_21_ABI24.ab1     1 CGTTTGTAAC ATCTGCTCAT GTTCATGCCT TCTTCTTTTT CCTACAGCTC CTGGGCAACG    61 TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT CCTCGAGCCA CCATGTCCAA   121 TTTACTGACC GTACACCAAA ATTTGCCTGC ATTACCGGTC GATGCAACGA GTGATGAGGT   181 TCGCAAGAAC CTGATGGACA TGTTCAGGGA TCGCCAGGCG TTTTCTGAGC ATACCTGGAA   241 AATGCTTCTG TCCGTTTGCC GGTCGTGGGC GGCATGGTGC AAGTTGAATA ACCGGAAATG   301 GTTTCCCGCA GAACCTGAAG ATGTTCGCGA TTATCTTCTA TATCTTCAGG CGCGCGGTCT   361 GGCAGTAAAA ACTATCCAGC AACATTTGGG GCCAGCTAAA ACATGCTTCA TCGTCGGGTC   421 CCGGGCTGCC ACGACCAAAG TGACAGCAAT GCTGTTTCAC TGGTTATGCG GGCGGATCCG   481 AAAAAAAAAC GTTGAAGGCC GGTGAAACGT TCAAAAAAGG CTTCTAGCGT TCGAACGCAC   541 TGAATTTCGA CCAAGTTCGT TTCCCCCTTG GAAAATAAGC GATCGCCGGC CAGAATAAAA   601 CGTAACCGGG ACTTTTTGGG GGATTTGCTT AAAAACCCCC GTGTTACGTA AGCCCAAAAT   661 TTCCCAGAAT CAGGGGTTAA AAAAATTTTC CCCTTCTGGC CGGGGGGAAA AAAGTTAATC   721 CCTTTTTGGC CAAAACAAAA AACCCTGGTT TCCCCCCCCG GGGGTAAAAA AGGCCCTTTA   781 CCCTGGGGGG AAAAAAAACG GGGCCCAGGC AGGGGATTCC CCCCCCTCGG GGGGACCCAA   841 AAATCCCCAA AAATTCCCGT GGTTTCTGCG GGGGCAAAAA AAATGGGTGT GTGCGCGCGC   901 ATATTGTCGC CACACGACAG TTTATACTCT CGCGCCCCGG GGAGGGGGGT ATTTTTTAAA   961 GACACCCTCC CCTAGTGTTT ACACGGCGCA CACGAGAGAA TACACTGCTC GTGCAGAAAA  1021 ACACTCGCGG CGGTGTGGGG AGAACACAAA TCTCCGCCTG TGATGAGGAG CACGCCCGCA  1081 GATGAGGTAG TAGCTCGCTC GTGATGATAA TATATCTACA CATCACGAAC AGCATGCGAT 1141 CGATG //
Primer: IRES-R Sequence file: RDB14288_A6Gqb.seq
>RDB12117_14288_A6Gq_IRES-R_G05_20_ABI24.ab1     1 GCAACGGCGG CTTCGGCCAG TACGTTAGGG GGGGGGGAGG GAGAGGGGGG GGGGGCGGAA    61 TTCGATATCA AGCTTATCGA GCGGCCGCTC ACTTTTGATG AAACAGAAGT TTTTTGATAT   121 TTCCATTTGA ATATTTTGGT ATCTGATTGG TGATGATTTC AGCTAACATC TCGGGGAACT   181 CGATGGACAT GGTCTTATCC AAAAATGTTT GGAAGCAATA GTTAAGGAGA TTTTCAACCA   241 CTTCATGCAT AGAATCCAAG AGTTTTGTCA GTTGATAAAA CCGCTGCCAG TTCTGGCTGG   301 AGTTTCCTTC CCTCTTGACA ATGGCTTTTC CTAGCTCTTT GATGTAGGTC ATTCTAATTT   361 CATCAAATAG CTCTTGGCTC TTCAGACCGT CCTTAGGAAC TGAAGAGAGA AGCAGTAAGG   421 TTTTCATACA GAGATACTCT TCATAAGATA CCTGAAGCCT GTGTAACTCA GAGGAAACAT   481 ACAGCATGTG TTTACATTGG TCGTACATGC AGGGTAGAGT CATTCTCTGC TCATTAATAA   541 TCAGATCAGG AGCAAAACAC AGCAGGTTTG CACTTGATTG TCTATATGAT CTCCACCCCA   601 GAGCAAATGC CATAAGAAAC ATCCAGGAGT ACTGCAGTAG GGTCATTTGG TCATCCAGGT   661 GTAAGTTCCT GAAACCTGGT ATTGCCTTTG CCCATTTCAC TGCTGCAATC ACTTGCCGCC   721 CTCCTAACAT GTTGAGCGTA GTCATGATCC TCCAAGTTGA GTCTGGAACA GAGCTATCAT   781 ATCCTGCATA TAACACTTCA GGTTCAATAA CCTCCAACAG TGACACCAGG GTAGGGGTGA   841 GTTGTGGTAA CGTTGCAGGA ACTATTGTTT TGTTACCAGG ATTTTCCATG GCATAATCGC   901 CATCTTCCAG CAAGGCGCAC CATTGCCCCT GTTTCACTAT CCCAGGTTAC GGATATAGTT   961 CATGACAATA TTTACATTGG TCCCAGCCCA CCAGCTTGCA TGAATCTCCG GTATTGAACT  1021 CCAGCGCGGG CCCATATCTC GCCGCGCTCG ACACGGGCAC TGTGTCCAGA ACAAGCCAGG 1081 TATCTCTGGA ACCAGAAGTT CATCATTAAG CGTCGGTAAT CAATCCGAAA ATGAAGCTG //
Primer: IRES-F Sequence file: RDB14288_A6Gqc.seq
>RDB12117_14288_A6Gq_IRES-F_D08_11_ABI24.ab1     1 GCAGTCATTC TGTGTTTAGT CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC    61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGCCTT TGTCTCAAGA   121 AGAATCCACC CTCATTGAAA GAGCAACGGC TACAATCAAC AGCATCCCCA TCTCTGAAGA   181 CTACAGCGTC GCCAGCGCAG CTCTCTCTAG CGACGGCCGC ATCTTCACTG GTGTCAATGT   241 ATATCATTTT ACTGGGGGAC CTTGTGCAGA ACTCGTGGTG CTGGGCACTG CTGCTGCTGC   301 GGCAGCTGGC AACCTGACTT GTATCGTCGC GATCGGAAAT GAGAACAGGG GCATCTTGAG   361 CCCCTGCGGA CGGTGCCGAC AGGTGCTTCT CGATCTGCAT CCTGGGATCA AAGCCATAGT   421 GAAGGACAGT GATGGACAGC CGACGGCAGT TGGGATTCGT GAATTGCTGC CCTCTGGTTA   481 TGTGTGGGAG GGTTAACTAG ATAAGTAATG ATCATAATCA GCCATATCAC ATCTGTAGAG   541 GTTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT   601 GCAATTGTTG TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC   661 ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA   721 CTCATCAATG TATCTTATCA TGTCTGGATC CGCTGCATTA ATGAATCGGC CAACGCGCGG   781 GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT   841 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA   901 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA   961 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT  1021 CACAAAAATC GGACGCTCAA AGTCAGGAGG TGGGCGAACC CCGGACCAGG AACTATAAGG  1081 AATACAGGCG GTTTCCCCCT GGAGCTCCCC TCGGTGCGCG CCTTCTCCTG GTTTCCGGAA 1141 CCCTTGGCCC CGCCCA //
Primer: Amp_R Sequence file: RDB14288_A6Gqd.seq
>RDB12117_14288_A6Gq_Amp_R_E08_14_ABI24.ab1     1 TTCGTTTCAT GACGCTTTGG AAACAAAGAC TGTATTTCCT GGAAATTAAT GTTTATTCAA    61 TAAACTGTGT ATTCAGCTAT ATTCACATAG TGGTGAGGCT GAAATGAGGC GGGAAGAGGC   121 GGTTGGGGCT TAATTATATC AATTTGGGTG GCCCCACAGC GCCTCCAAGG CGCCAGTCCT   181 GTTTTGACAA GTTGCCTCTG GAAGCCTCTC TTCTTTTTCT CCAGAGTAAG CGGAGGCCAG   241 GGGCCCCCGG CCTCTGCTTA ATACTAAAAA AAACAGCTGT TGTCATAGTA ATGATTGGGT   301 GGAAACATTC CAGGCCTGGG TGGAGAGGCT TTTTGCTTCC TCTTGCAAAA CCACACTGAC   361 ATTCCAGGCC TGGGTGGAGA GGCTTTTTGC TTCCTCTTGC AAAACCACAC TGCCCTCTGG   421 AGGGCAGTTG CCTAGCAACT AATTAAAAGA GGATGTCGCA CGGCCAGCTG CGGTCAGTTA   481 GTCACTTCCT GCTTAACTGA CTTGACATTT TCTATTTTAA GAGTCGGGAG GAAAATTACT   541 GTGTTGGAGG CCCTCCGCCA TCTTCTGAAG CTGAATCGAA TTAACTTGTT TATTGCAGCT   601 TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTTC   661 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCTG   721 ATATCATCGT CGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT   781 AGTTCATAGC CCAATATAAT GGAGTTTCCG CGTTAACATA ACTTACGTAA TGACCCGCCT   841 GCTGACCGCC CAACGACCCC CGCCATGACG TCAATATGAC GTATGTTCCA TAGTACGCCA   901 ATAGGGACTT CATGACGTCA TGGTTGGAAG TATTACGTAA CTGGACACTG CCAGTACATT   961 CAGTGTATCC TATGGCAGTA CGCCCATTGA CTCATGACGT ATTGCGCTGC ATAGCCAGTA 1021 CATGACTATG GACTG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.01.19

GNP_filter3_RDBDEP_html_240118.pl