Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMYs-Tead2FL-IRES-EGFP

Retroviral vector of mouse Tead2

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infections virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。 付加的提供条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19898_B3Bap1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352) Region: pol region,insert 5' Sequence file: RDB19898_B3Baa.seq
>12173_19898_B3Ba_3_MMLV-pol_F_B04_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGG NNTCGCAGCT TGGNNACNCG CCGCCCACGT GAAGGCTGCC    61 GACCCCGGGG GTGGACCATC CTCTAGACTG CCGGATCTAG CTAGTTAATT AAGGATCCCA   121 GTGTGGTGGT ACGGGAATTC GAGGCCCAGA TGGGGGATCC CCGGACTGGG GCCCCCCTGG   181 ATGATGGCGG GGGCTGGACA GGTAGCGAGG AAGGCAGCGA AGAGGGCACC GGAGGCAGCG   241 AGGGCGTTGG AGGTGATGGC AGCCCTGATG CAGAAGGTGT GTGGAGCCCC GACATTGAGC   301 AGAGTTTTCA GGAGGCCCTG GCCATCTACC CTCCCTGTGG CCGTCGCAAG ATCATCCTGT   361 CAGATGAGGG CAAGATGTAT GGCCGGAACG AACTCATTGC CCGTTACATC AAGCTGAGGA   421 CAGGGAAGAC GAGAACGCGA AAGCAGGTCT CCAGCCATAT TCAGGTTTTG GCTCGAAGGA   481 AATCGAGAGA AATTCAGTCC AAGCTGAAGG ACCAAATCTC CAAGGACAAG GCCTTCCAGA   541 CGATGGCCAC CATGTCTTCG GCACAGCTCA TCTCCGCCCC TTCCCTCCAG GCCAAGCTGG   601 GCCCTTCTGG CCCTCAGGCC ACTGAGCTTT TCCAGTTCTG GTCAGGGAGC TCTGGGCCAC   661 CATGGAATGT TCCAGACGTG AAGCCCTTCT CACAGGCACC GTTCTCCGTG TCACTGACGC   721 CNCCAGCCTC TGACCTACCA GGGTACGAGC CGCCCCCAGC CCTCTCACCC CTGCCCCCAC   781 CCGCTNCGTC TCCCCCAGCC TGGCANGCTC NGGCCCTGGG CACTGCCCGC CTGCANCTGA   841 NAGANTTCTC AGCGTTTGTG GAANCGCCAG ANNCANNTNN NTCNTTNCAN ANNNNTCTGN 901 TTGNNCNCAT CANNCAGNNN NNNNNNN //
Primer: Tead2FL-B_F (Pr1296) Region: insert 3',IRES2 Sequence file: RDB19898_B3Bab.seq
>12173_19898_B3Ba_3_Tead2FL-B_F_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNCNNN NNNAGGTCTG CTCCTTTGGC AAGCAAGTGG TAGAGAAGGT    61 GGAGACGGAA CGGGCCCAGC TGGAGGACGG GCGCTTTGTG TACCGTCTGC TGCGCTCTCC   121 CATGTGTGAG TACCTGGTTA ATTTCCTGCA CAAGCTCCGT CAGCTGCCTG AACGCTACAT   181 GATGAACAGT GTCCTGGAGA ACTTCACCAT CCTCCAGGTT GTGACAAACA GGGACACTCA   241 GGAACTGCTG CTGTGTACTG CCTACGTCTT TGAAGTCTCC ACCAGTGAAC GAGGAGCCCA   301 GTACCACATC TACCGCCTGG TCAGGGACTG ATCTCGAGGG CCGGCGCGCC GCGGCCGCTA 361 CGTAAATTCC GCCCCCCCCC CNNNNNNNNN N //
Primer: IRES-F (Pr0036) Region: IRES2,EGFP 5' Sequence file: RDB19898_B3Bac.seq
>12173_19898_B3Ba_3_IRES-F_D03_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTTN NGTCNAGGTN NNAAAAACGT CTAGGCCCCC CGAACCACGG    61 GGACGTGGTT TTCCTTTGAA AAACACGATG ATAATATGGC CACAACCATG GTGAGCAAGG   121 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG   181 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC   241 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC   301 TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT   361 TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG   421 GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG   481 AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA   541 ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA   601 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC   661 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC   721 AGTCCGNCCT GANCAAAGAN NNCNACGAGA AGCGCGATCA CNTGGTCCTG CTGNAGTTCG   781 TGANCGCCGC CGGGNTCACT CTCNGNNTGG NNNANCTGNN CNNGTAAAGN GGNCGGNCGC   841 CAGCACANNN NNCNANNGGG NCGNCNGCNN ANNGGNCNAT CGAGGGGGGG NCNNNGANTA 901 NNCCNNTTNG NNNAANANNG NNNNTNNNNN NNNNAGGNNC NANTTTTGAN NNNNNNNNN //
Primer: Amp_R (Pr0008) Region: AmpR pro,5' LTR,psi Sequence file: RDB19898_B3Bad.seq
>12173_19898_B3Ba_3_Amp_R_E03_15_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN TTGNAGNNTT TATNNGGGTT ATTGTCTCNT GAGCGGATAC    61 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA   121 GTGCCACCTG CATAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC   181 CATTTTGCAA GGCATGGAAA AATACATAAC TGAGAATAGA AAAGTTCAGA TCAAGGTCAG   241 GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC   301 CCGGCTCAGG GCCAAGAACA GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG   361 TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA GAACAGATGG TCCCCAGATG CGGTCCAGCC   421 CTCAGCAGTT TCTAGAGAAC CATCAGATGT TTCCAGGGTG CCCCAAGGAC CTGAAATGAC   481 CCTGTGCCTT ATTTGAACTA ACCAATCAGT TCGCTTCTCG CTTCTGTTCG CGCGCTTCTG   541 CTCCCCGAGC TCAATAAAAG AGCCCACAAC CCCTCACTCG GCGCGCCAGT CCTCCGATTG   601 ACTGAGTCGC CCGGGTACCC GTATTCCCAA TAAAGCCTCT TGCTGTTTGC ATCCGAATCG   661 TGGACTCGCT GATCCTTGGG AGGGTCTCCT CAGATTGATT GACTGCCCAC CTCGGGGGTC   721 TTTCATTTGG AGGTTCCACC GAGATTTGGA GACCCCTGCC CAGGGNCCAC CGACCCCCCC   781 GCCGGGAGGT AAGCTGGNCA GCGGTCGTTT CGTGTCTGTC TCTGTCTTTG TGCGTGTTTG   841 TGNCGGCATC TAATGNTTGC GCCTGCGTCN GTNCTANTTA GCTNACTAGC TCTGTATCTG   901 GNGNANCCGT GNNGGAACTG ANNANTTCGG AACACCCGGN NGCNNCCCTG GGNNANGNCC   961 CNNGNANTTC NGGGGNNNNT TTTGNGGNCC GANNNNANNN NNAAATCCCN ATNGNTTTNN 1021 NNNNNTNNNN GNNNCCCCNN NNNNNNNN //
Primer: pMFG_1 (Pr0230) Region: pol region,psi Sequence file: RDB19898_B3Bae.seq
>12173_19898_B3Ba_3_pMFG_1_D04_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNT NANTNNNTCT TGTNNTGTCN GGGTCAGGGA AGTTTACNAG    61 GGGCGGGGGT GCCCCATATA AGATCTCATA TGGCCATATG GGGGCGCCTA GAGAAGGAGT   121 GAGGGCTGGA TAAAGGGAGG ATCGAGGCGG GGTCGAACGA GGAGGTTCAA GGGGGAGAGA   181 CGGGGCGGAT GGAGGAAGAG GAGGCGGAGG CTTAGGGTGT ACAAAGGGCT TGACCCAGGG   241 AGGGGGGTCA AAAGCCAAGG CTTCCCAGGT CACGATGTAG GGGACCTGGT CTGGGTGTCC   301 ATGCGGGCCA GGTGAAAAGA CCTTGATCTT AACCTGGGTG ATGAGGTCTC GGTTAAAGGT   361 GCCGTCTCGC GGCCATCCGA CGTTAAAGGT TGGCCATTCT GCAGAGCAGA AGGTAACCCA   421 ACGTCTCTTC TTGACATCTA CCGACTGGTT GTGAGCGATC CGCTCGACAT CTTTCCAGTG   481 ACCTAAGGTC AAACTTAAGG GAGTGGTAAC AGTCTGGCCC TAATTTTCAG ACAAATACAG   541 AAACACAGTC AGACAGAGAC AACACAGAAC GATGCTGCAG CAGACAAGAC GCGCGGCGCG   601 GCTTCNGTCC CNAACCGAAA GCAAAAATTC NGACNGANGC GGGAACTGTT TTANGTTCTC   661 GTCTNCTANN NGANNCACNT ATCCNTCCTA TTNNGGGGGN GNANCNNAGA NTCCAAAACG   721 ATCGGGANTT TTGGANTCNN NCNGGNNNCN AAAACNGNCN NCNANNTCCN TGGGNNGNCT 781 CCNNNNNNNN NN //
Primer: mVenus-M_F (Pr0574) Region: EGFP 3',3' LTR Sequence file: RDB19898_B3Baf.seq
>12173_19898_B3Ba_3_mVenus-M_F_G04_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGNN TCNNGGTNAN TTCNNGANCC GCCNCAACAT CGAGGACGGC    61 AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG   121 CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG   181 CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC   241 GAGCTGTACA AGTAAAGCGG CCGGCCGCCA GCACAGTGGT CGACCGGGCC GCCAGCACAG   301 TGGTCGATCG AGGGGGGGCC CGCGATTAGT CCAATTTGTT AAAGACAGGA TATCAGTGGT   361 CCAGGCTCTA GTTTTGACTC AACAATATCA CCAGCTGAAG CCTATAGAGT ACGAGCCATA   421 GATAGAATAA AAGATTTTAT TTAGTCTCCA GAAAAAGGGG GGAATGAAAG ACCCCACCTG   481 TAGGTTTGGC AAGCTAGCTT AAGTAACGCC ATTTTGCAAG GCATGGAAAA TACATAACTG   541 AGAATAGAGA AGTTCAGATC AAGGTTAGGA ACAGAGAGAC AGCAGAATAT GGGCCAAACA   601 GGATATCTGT GGTAAGCAGT TCCTGNCCCG GCTCANGGCC NAGAACAGTT GGAACAGCAG   661 AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCN NGNCCAAGAA   721 CAGATGGTCC CCAGATGCGG NCCCGCCCTC ANCANTTTCT AGANAANCAT CANATGNTTC   781 NNGGGNGNCC CNNNNACCTG AAATGANCNT GNNNNTTATT TGANNTNNCN NANCANNNCG   841 NTTCNCGNTT CNNNNCNNNN GNNNNNGNNN NNCNANNNNN NNNAAANANN NNNNNANNNN 901 NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

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