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pMYs-Tead2-VP16-IRES-EGFP (#RDB12174)

Retroviral vector of mouse Tead2, constitutive active

Clone info. Retroviral vector of mouse Tead2, activation domain of herpes simplex virus VP16, constitutive active. cDNA fragment 87 to 1,424 nt of NM_011565.2 (CDS, 87..1424) is cloned.
Comment Transfection can be confirmed by GFP. See Fig. 2B of Ota and Sasaki, Development 135: 4059-4069 (2008). Known differences: G to A substitution at 453 nt (Val to Ile at 123rd aa).
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 6.2 kb
Selectable markers Amp^r
Gene/insert name Mouse Tead2 cDNA HSV VP16 protein cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infections virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12174 pMYs-Tead2-VP16-IRES-EGFP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMYs-Tead2-VP16-IRES-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12174).

Reference section:

Ota, M., Sasaki, H., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19899_B3Bap1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(Tead2 1-127aa)
Sequence file: RDB19899_B3Baa.seq check
>12174_19899_B3Ba_1_MMLV-pol_F_B05_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG GCNTCGNAGC TTGGNNNCNC GCCGCCCACG TGAAGGCTGC
   61 CGACCCCGGG GGTGGACCAT CCTCTAGACT GCCGGATCTA GCTAGTTAAT TAAGGATCCC
  121 AGTGTGGTGG TACGGGAATT CCTGCAGGAG CTATTTAGGT GACACTATAG AATACAAGCT
  181 ACTTGTTCTT TTTGCAGGAT CCCATCGATT CGAATTCGAG GCCCAGATGG GGGATCCCCG
  241 GACTGGGGCC CCCCTGGATG ATGGCGGGGG CTGGACAGGT AGCGAGGAAG GCAGCGAAGA
  301 GGGCACCGGA GGCAGCGAGG GCGTTGGAGG TGATGGCAGC CCTGATGCAG AAGGTGTGTG
  361 GAGCCCCGAC ATTGAGCAGA GTTTTCAGGA GGCCCTGGCC ATCTACCCTC CCTGTGGCCG
  421 TCGCAAGATC ATCCTGTCAG ATGAGGGCAA GATGTATGGC CGGAACGAAC TCATTGCCCG
  481 TTACATCAAG CTGAGGACAG GGAAGACGAG AACGCGAAAG CAGGTCTCCA GCCATATTCA
  541 GGTTTTGGCT CGAAGGAAAT CGAGAGAAAT TCAGTCCAAG CTGAAGGACC AAATCTCCAA
  601 GGACAAGGAN TTCCCGGGGA TCTGGGCCCC CCCGACCGAT GTCAGCCTGG GGGACGAGCT
  661 CCACTTAGAC GGCGAGGACG TGGCGATGGC GCATGCCGAC GCGCTAGACG ATTTCGATCT
  721 GGACNTGTTG GGGGANGGNN NTTCCCCGGN NCCGGGATTT ACNNNNCACG ACTCCGCCCC
  781 CTACNGCGCT CTGGATATGG NCNANTNCNN NTTTGANCAG ATGTTTACCG ATGCCNNNNN
  841 NANNTGACNA NNACNGNGGG NANGNNTCGA GGNNCNGNNN NNNNNNGNNN NCTACNNNNN
901 TNNNNNNNNN N
//
Primer: Tead2-B_R (Pr1297)
Region: insert 3'(Tead2 1-127aa 3',VP16 AD),IRES
Sequence file: RDB19899_B3Bab.seq check
>12174_19899_B3Ba_1_Tead2-B_R_B01_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCNGC NNTATTNNGG TTTTGGCTCG AAGGAAATCG AGAGAAATTC
   61 AGTCCAAGCT GAAGGACCAA ATCTCCAAGG ACAAGGAATT CCCGGGGATC TGGGCCCCCC
  121 CGACCGATGT CAGCCTGGGG GACGAGCTCC ACTTAGACGG CGAGGACGTG GCGATGGCGC
  181 ATGCCGACGC GCTAGACGAT TTCGATCTGG ACATGTTGGG GGACGGGGAT TCCCCGGGTC
  241 CGGGATTTAC CCCCCACGAC TCCGCCCCCT ACGGCGCTCT GGATATGGCC GACTTCGAGT
  301 TTGAGCAGAT GTTTACCGAT GCCCTTGGAA TTGACGAGTA CGGTGGGTAG GCCTCGAGGG
  361 CCGGCGCGCC GCGGCCGCTA CGTAAATTCC GCCCCCCCCC CCCCTCTCCC TCCCCCCCCC
  421 CTAACGTTAC TGGCCGAAGC CGCTTGGAAT AAGGCCGGTG TGCGTTTGTC TATATGTTAT
  481 TTTCCACCAT ATTGCCGTCT TTTGGCAATG TGAGGGCCCG GAAACCTGGC CCTGTCTTCT
  541 TGACGAGCAT TCCTAGGGGT CTTTCCCCTC TCGCCAAAGG AATGCAAGGT CTGTTGAATG
  601 TCGTGAAGGA AGCAGTTCCT CTGGAAGCTT CTTGAAGACA AACAACGTCT GTAGCGACCC
  661 TTTGCAGGCA GCGGAACCCC CCACCTGGCG ACAGGTGCCT CTGCGGCCAA AAGCCACGTG
  721 TATAAGATAC ACCTGCNAAG GCGGCACAAC CCCAGTGCCA CGTTGTGAGT TGGATAGTTG
  781 TGGAAAGAGT CAAATGGCTC TNCTCNAGCG TATTCAACAN NGGGCTGAAN GATGNCCANA
  841 ANGNNNCCCA TTGNATNGGA TCTGATCTGG GGNCTCNGNG CNCNTGCTTT NCNTGTGTTT
  901 AGTCNANGNT NAAAAAACGT CTAGGCCCCC CNNANCNNNG GGNNGNGNNT TNNCTTNNAA
961 AAANNNNANG ANNNNANNGN NNNNNNNNN
//
Primer: IRES-F (Pr0036)
Region: IRES,EGFP 5'
Sequence file: RDB19899_B3Bac.seq check
>12174_19899_B3Ba_1_IRES-F_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNAGGT TAAAAAAACG TCTAGGCCCC CCGAACCACG
   61 GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GGTGAGCAAG
  121 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC
  181 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC
  241 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC
  301 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
  361 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC
  421 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC
  481 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC
  541 AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG
  601 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG
  661 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCNCC
  721 CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC
  781 GTGANCGCCG CCGGNATCAC TCTCGGNNTG GANNANCTGT ACAAGTAAAG CGGNNGGNNG
  841 CCAGCACAGN GGTCGANCGG NNCNCCNGCN CNNNGGNCNA TCGANGGGGG GNCCGCGANN
  901 NNTCCNATTT GNNAAAGANN GGATATCNNN NNNNCNNNNT CNNNNTTNNN TCNNCNNNNT
961 NNNNNGNTNN NNNNNNNN
//
Primer: Amp_R (Pr0008)
Region: AmpR pro,5'LTR
Sequence file: RDB19899_B3Bad.seq check
>12174_19899_B3Ba_1_Amp_R_F05_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTTG NNGCNTTTAT NNGGGTTATT GTCTCATGAG CGGATACATA
   61 TTTGAATGTA TTTAGAAAAA NNNNNNNATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG
  121 CCACCTGCAT AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT
  181 TTTGCAAGGC ATGGAAAAAT ACATAACTGA GAATAGAAAA GTTCAGATCA AGGTCAGGAA
  241 CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG
  301 GCTCAGGGCC AAGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA
  361 GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC
  421 AGCAGTTTCT AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT
  481 GTGCCTTATT TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC
  541 CCCGAGCTCA ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATTGACT
  601 GAGTCGCCCG GGTACCCGTA TTCCCNATAA AGCCTCTTGC TGTTTGCATC CGAATCGTGG
  661 ACTCGCTGAT CCTTGNNAGG GTCTCCTCAG ATTGATTGAC TGCNNACCTC GGGGGNCTTT
  721 CATTTGNAGG NTCNACCGAN ATTNGGAGAN CCCTGCCNNG GGNNNNCNGA CCCCNCNNNN
  781 NGGAGGNANG CNNGNCNNGC GNNCGNTTCN NGNCTGNNNN TGNCNTNGNN NGNGNTNNNG
841 NNNNNNTNNN NNNNTNNNNN CNGNGNCNGN NCNANTNAGN TNNNTANNNN NNNNNN
//
Primer: pMFG_1 (Pr0230)
Region: pol region,psi
Sequence file: RDB19899_B3Bae.seq check
>12174_19899_B3Ba_1_pMFG_1_D05_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NTNNNNNNNC TTGTNNTGTC AGGGTCAGGG NAGTTTACAA
   61 GGGGCGGGGG TGCCCCATAT AAGATCTCAT ATGGCCATAT GGGGGCGCCT AGAGAAGGAG
  121 TGAGGGCTGG ATAAAGGGAG GATCGAGGCG GGGTCGAACG AGGAGGTTCA AGGGGGAGAG
  181 ACGGGGCGGA TGGAGGAAGA GGAGGCGGAG GCTTAGGGTG TACAAAGGGC TTGACCCAGG
  241 GAGGGGGGTC AAAAGCCAAG GCTTCCCAGG TCACGATGTA GGGGACCTGG TCTGGGTGTC
  301 CATGCGGGCC AGGTGAAAAG ACCTTGATCT TAACCTGGGT GATGAGGTCT CGGTTAAAGG
  361 TGCCGTCTCG CGGCCATCCG ACGTTAAAGG TTGGCCATTC TGCAGAGCAG AAGGTAACCC
  421 AACGTCTCTT CTTGACATCT ACCGACTGGT TGTGAGCGAT CCGCTCGACA TCTTTCCAGT
  481 GACCTAAGGT CAAACTTAAG GGAGTGGTAA CAGTCTGGCC CTAATTTTCA GACAAATACA
  541 GAAACACAGT CAGACAGAGA CAACACAGAA CGATGCTGCA GCAGACAAGA CGCGCGGCGC
  601 GGCTTCGGTC CCAAACCGAA AGCAAAAATT CAGACGGAGG CGGGAACTGT TTTAGGTTCT
  661 CGTCTCCTAC CAGAACCACA TATCCCTCCT ATTAGGGGGG TGCACCAAAG AGTCCAAAAC
  721 GATCGGGATT TTTGGANTCN NGTCGGGCCN CAAAAACGGC CCCCGAAGTC CCTGGGNNGT
  781 CTCCCAGGGT TGCGGNCGGG TGTTCCNAAC TCGTCAGTTC CACCACGGGT CCGCCNGANA
  841 CAGANCTNGT TAGCTAACTA GTACAGACGC NNNGCAAACN TTANATGCCN GNNCNAACNC
901 NCACNAAGNC NNANNNNNAN NCNAAACNAN NNCNNGNNNN NNNTNNNNNN NNNN
//
Primer: mVenus-M_F (Pr0574)
Region: EGFP 3',3'LTR
Sequence file: RDB19899_B3Baf.seq check
>12174_19899_B3Ba_1_mVenus-M_F_G05_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGCN TCNAGGTGAN TTNNAGANCC GCCACAACAT CGAGGANGGC
   61 AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG
  121 CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG
  181 CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC
  241 GAGCTGTACA AGTAAAGCGG CCGGCCGCCA GCACAGTGGT CGACCGGGCC GCCAGCACAG
  301 TGGTCGATCG AGGGGGGGCC CGCGATTAGT CCAATTTGTT AAAGACAGGA TATCAGTGGT
  361 CCAGGCTCTA GTTTTGACTC AACAATATCA CCAGCTGAAG CCTATAGAGT ACGAGCCATA
  421 GATAGAATAA AAGATTTTAT TTAGTCTCCA GAAAAAGGGG GGAATGAAAG ACCCCACCTG
  481 TAGGTTTGGC AAGCTAGCTT AAGTAACGCC ATTTTGCAAG GCATGGAAAA TACATAACTG
  541 AGAATAGAGA AGTTCAGATC AAGGTTAGGA ACAGAGAGAC AGCAGAATAT GGGCCAAACA
  601 GGATATCTGT GGTAAGCAGT TCCTGCCCCG GCTCANGGCC AAGAACAGTT GGAACAGCAG
  661 ANTATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCN NGGCCAAGAA
  721 CAGATGGNCC CCANATGCGG NCCCGCCCTC ANCAGTTTCT ANAGAANCAT CANATGTTTN
  781 NNGGGNGNCC NNNNNNNCTG AANTGACCCT NNNNNNNANT TGAANTNANC NATCANNNCG
841 NTTCNCNCNN NNNNNCNNGN NNNNNNGNNN CNNNANNNNN NNAAAANANN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]
user_report Zhang, J., TEA Domain Transcription Factor 4 Is the Major Mediator of Yes-Associated Protein Oncogenic Activity in Mouse and Human Hepatoblastoma. Am. J. Pathol. pii: S0002-9440 (18) 30776-4 (2019). PMID 30794805. [link to RRC of NBRP]