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pMYs-HA-Yap1-IRES-EGFP

Retroviral vector of mouse Yap1

Catalog number RDB12177
Resource name pMYs-HA-Yap1-IRES-EGFP
Clone info. Retroviral vector of mouse Yap1. cDNA fragment 210 to 1,625 nt of NM_009534.3 (CDS, 207..1625) is cloned.
Comment Transfection can be confirmed by GFP. See Fig. 3A of Ota and Sasaki, Development 135: 4059-4069 (2008). Known differences: G to C substitution at 212 nt (Glu to Asp at 2nd aa). C to A substitution at 477 nt (Arg at 91st aa, synonymous) and XhoI site insertion at the position.
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r
Gene/insert name Mouse Yap1 cDNA
Depositor|Developer Sasaki, Hiroshi |
Other clones in our bank

External Database
Mouse Yap1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
          Additional terms and conditions:
          Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infections virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
          付加的提供条件:
          pMXsシリーズベクターについて:利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB12177 pMYs-HA-Yap1-IRES-EGFP DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pMYs-HA-Yap1-IRES-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12177).

          Reference section:

          Ota, M., Sasaki, H., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12177_A6Ajp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: 5'Retro (pFB-5')
          Sequence file: RDB12177_A6Aja.seq check
          >12177_12177_A6Aj_5'Retro(pFB-5')_C09_09.ab1
              1 GAGCGGGAGG GAATTCTTAT CGGACTGCTG TTTATTAGGA TCCCAGTGTG GTGGTACGGG
             61 AATTCCTGCA GGGGGCTAGC GAATTCGATT AATACGACTC ACTATAGGGA GACCCAAGCT
            121 TGCCGCCACC ATGTACCCAT ACGACGTTCC AGATTACGCT GGTACCGACC CCGCGCAACA
            181 GCCGCCGCCC CAGCCGGCCC CGCAAGGCCC CGCGCCGCCG TCCGTGTCTC CGGCCGGGAC
            241 CCCCGCGGCC CCGCCCGCAC CCCCGGCCGG CCACCAGGTC GTGCACGTCC GCGGGGACTC
            301 GGAGACCGAC TTGGAGGCGC TCTTCAATGC CGTCATGAAC CCCAAGACGG CCAACGTGCC
            361 TCAGACCGTG CCCATGCGGC TTCGCAAGCT GCCCGACTCC TTCTTCAAGC CGCCTGAGCC
            421 CAAGTCCCAC TCGAGACAGG CCAGTACTGA TGCAGGTACT GCGGGAGCTC TGACTCCACA
            481 GCATGTTCGA GCTCACTCCT CTCCAGCCTC CCTGCAGCTG GGTGCCGTTT CTCCTGGGAC
            541 ACTCACAGCC AGTGGCGTTG TCTCTGGCCC TGCCGCTGCC CCTGCAGCTC AGCATCTCCG
            601 GCAGTCCTCC TTTGAGATCC CTGATGATGT ACCACTGCCA GCAGGCTGGG AGATGGCCAA
            661 GACATCTTCT GGTCAAAGAT ACTTCTTAAA TCACAACGAT CAGACAACAA CATGGCAGGA
            721 CCCCCGGAAG GCCATGCTTT CGCAACTGAA CGTTCCTGCG CCTGCCAGCC CAGCGGTGCC
            781 CCAGACGCTG ATGAATTCTG CCTCAGGACC TCTTCCTGAT GGATGGGAGC AAGCCATGAC
            841 TCAGGATGGA GAAGTTTACT ACATAAACCA TAAGAACAAG ACCACATCCT GGCTGGACCC
            901 AAGGGCTGGA CCCTCGTTTT GCCATGAACC AGAGGATCAC TCAGAGTGCT CCAGTGAAGC
            961 AGCCCCCACC CTTGGCTCCC CCAGAGCCCA CAGGGAGGCG TTCCTGGGTG GAGGCAGTTT
           1021 CCAAACCAGC AGCAGCAAAT ACAGCTGCAG CAGTTACAGA TGGAGAGGAG AGACTGCGGT
           1081 TGAAACAACA GGATTATTTT CGGCAGATTT AGCTCTTGCG CAGCCAGTTG GCTTACACTG
          1141 GGAGCCAAGG AATGGAAGGG AAACT
          //
          Primer: IRES-R
          Sequence file: RDB12177_A6Ajb.seq check
          >12177_12177_A6Aj_IRES-R_B09_06.ab1
              1 GCAACGGGCG GCTTCGGCCA GTACGTTAGG GGGGGGGGAG GGAGAGGGGG GGGGGGGGCG
             61 GAATTTACGT AGCGGCCGCG GCGCGCCGGC CCTCGAGGAT CTGCAGAATT CCAGCACACT
            121 GGCGGCCGTT ACTAGTGGAT CCCTCACAGA CTCAGAGTGG CTCCCTGCAG CTCTATAACC
            181 ACGTGAGAAA GCTTTCTTTA TCTAGCTTGG TGGCAGCCAA CACAGACTCC ACGTCCAAGA
            241 TTTCGGAACT CAGCGCTTCC TGCAGACTGG GCATCAGCTC CTCCCCTTCT ATGTTCATTG
            301 CATCTCCTTC CAGTGTGCCA AGGTCCACAT TTGTCCCAGG GAGGGCTTCC AGGTAGTCGG
            361 GGAAGCGGCT CTGCTGTGAC GGCAGGGTGC TTTGGCTGAT GGTGTCTCCT GTATCCATTT
            421 CATCCACACT GTTGAGGAAG TCGTCTGGGG TCCGAGGGAT GCTGTAGCTG CTCATGCTGA
            481 GGCCGCTGTC TGTGCTCTCA TCTCGAGAGT GATAGGTGCC ACTGTTAAGA AAGGGATCGG
            541 AACTATTGGT TGTCATTGTT CTCAATTCCT GAGACATCCC AGGAGAAGAC ACTGCATTCG
            601 GAGTCCCTCC ATCCTGCTCC AGTGTAGGCA ACTGGCTGCG CAGAGCTAAT TCCTGCCGAA
            661 ATAATTCCTG TTGTTTCAAC CGCAGTCTCT CCTTCTCCAT CTGTAACTGC TGCAGCTGTA
            721 TTTGCTGCTG CTGGTTGGAA CTGCCTCCAC CCAGGACGCC TCCCTGTGGG CTCTGGGGAG
            781 CCAAGGGTGG GGGCTGCTTC ACTGAAGCAC TCTGAGTGAT CCTCTGGTTC ATGGGCAAAA
            841 CGAGGGTCCA GCCTTGGGGT CCAGCCAGGA TGTGGTCTTT GTTCTTTATG GTTTATGTAG
            901 TAAACTTCTC CATCCTGAAG TCGTGGGCTT TGCTCCCATC CATCAGGAAA AAGGTCCTGA
            961 AGGCAAAATT CATCACCGTT CTGGGGGCAA CCGCTGGGCT GGCAGGCGCA AGAACCGTTT
          1021 CCATTTGCGA AAGCATTGGC CCTTTCCGGG GGG
          //
          Primer: IRES-F
          Sequence file: RDB12177_A6Ajc.seq check
          >12177_12177_A6Aj_IRES-F_D09_12.ab1
              1 GCATCGTTAT GTGTTTAGTC GAGGTTAAAA ACGTCTAGGC CCCCCGAACC ACGGGGACGT
             61 GGTTTTCCTT TGAAAAACAC GATGATAATA TGGCCACAAC CATGGTGAGC AAGGGCGAGG
            121 AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA
            181 AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT
            241 TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT
            301 ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT
            361 CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT
            421 ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA
            481 AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA
            541 ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA
            601 AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA
            661 CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG
            721 CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG
            781 CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTA AAGCGGCCGG CCGCCAGCAC
            841 AGTGGTCGAC CGGGCCGCCA GCACAGTGGT CGATCGAGGG GGGGCCCGCG ATTAGTCCAA
            901 TTTGTTAAAG ACAGGATATC AGTGGTCCAG GCTCTAGTTT TGACTCAACA ATATCACCAG
            961 CTGAAGCCTA TAGAGTACGA GCCATAGATA GAATAAAAGA TTTTATTTAG TCTCCAGAAA
           1021 AATGGGGGGA AATGAAAGAC CCCACCTGTA GTTTTGGCAA GCTAGCCTCA GTACGCATTT
          1081 TGCAGGCATG CAAATACGTA CCTGAGATAG AGTAGTTCAG ATCAAGGGTT AGGACCG
          //
          Primer: Amp_R
          Sequence file: RDB12177_A6Ajd.seq check
          >12177_12177_A6Aj_Amp_R_E09_15.ab1
              1 CTCTTTTCCA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
             61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
            121 ACCTGCATAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT
            181 TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAAAAGT TCAGATCAAG GTCAGGAACA
            241 GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC
            301 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC
            361 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG
            421 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT
            481 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC
            541 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATTGACTGA
            601 GTCGCCCGGG TACCCGTATT CCCAATAAAG CCTCTTGCTG TTTGCATCCG AATCGTGGAC
            661 TCGCTGATCC TTGGGAGGGT CTCCTCAGAT TGATTGACTG CCCACCTCGG GGGTCTTTCA
            721 TTTGGAGGTT CCACCGAGAT TTGGAGACCC CTGCCCAGGG ACCACCGACC CCCCCGCCGG
            781 GAGGTAAGCT GGCCAGCGGT CGTTTCGTGT CTGTCTCTGT CTTTGTGCGT GTTTGTGCCG
            841 GCATCTAATG TTTGCGCCTG CGTCTGTACT AGTTTAGCTA ACTAGCTCTG TATCTGGCGG
            901 ACCCGTGGTG GAACTGACGA GTTCGGAACA CCCGGGCCGC AACCCTGGGA GACGTCCCAG
            961 GGACTTCGGG GGGCCGTTTT TGTGGCCCGA CCTGAGTCCA AAAAATCCCG ATCGTTTTGG
           1021 ACTCTTTGTT GCACCCCCCC CTAATAAGAG GGATTATTGT GGATTTCTTG GGTAGGAAGA
           1081 CGAGACTTAA AACAGGTTCC CGCCTTTCGT CTGATTTTTG GCTTTCGGTT TTGGGACCGA
          1141 AGCCGCGCCC GAGCCGCATC CTTTGGAC
          //
          Primer: pMFG_1
          Sequence file: RDB12177_A6Aje.seq check
          >12177_12177_A6Aj_pMFG-1_F09_18.ab1
              1 GACATGGGGA GAGGGGCTGT TAGTAACTCT TGTCATGTCA GGGTCAGGGA AGTTTACAAG
             61 GGGCGGGGGT GCCCCATATA AGATCTCATA TGGCCATATG GGGGCGCCTA GAGAAGGAGT
            121 GAGGGCTGGA TAAAGGGAGG ATCGAGGCGG GGTCGAACGA GGAGGTTCAA GGGGGAGAGA
            181 CGGGGCGGAT GGAGGAAGAG GAGGCGGAGG CTTAGGGTGT ACAAAGGGCT TGACCCAGGG
            241 AGGGGGGTCA AAAGCCAAGG CTTCCCAGGT CACGATGTAG GGGACCTGGT CTGGGTGTCC
            301 ATGCGGGCCA GGTGAAAAGA CCTTGATCTT AACCTGGGTG ATGAGGTCTC GGTTAAAGGT
            361 GCCGTCTCGC GGCCATCCGA CGTTAAAGGT TGGCCATTCT GCAGAGCAGA AGGTAACCCA
            421 ACGTCTCTTC TTGACATCTA CCGACTGGTT GTGAGCGATC CGCTCGACAT CTTTCCAGTG
            481 ACCTAAGGTC AAACTTAAGG GAGTGGTAAC AGTCTGGCCC TAATTTTCAG ACAAATACAG
            541 AAACACAGTC AGACAGAGAC AACACAGAAC GATGCTGCAG CAGACAAGAC GCGCGGCGCG
            601 GCTTCGGTCC CAAACCGAAA GCAAAAATTC AGACGGAGGC GGGAACTGTT TTAGGTTCTC
            661 GTCTCCTACC AGAACCACAT ATCCCTCCTA TTAGGGGGGT GCACCAAAGA GTCCAAAACG
            721 ATCGGGATTT TTGGACTCAG GTCGGGCCAC AAAAACGGCC CCCGAAGTCC CTGGGACGTC
            781 TCCCAGGGTT GCGGCCGGGT GTTCCGAACT CGTCAGTTCC ACCACGGGTC CGCCAGATAC
            841 AGAGCTAGTT AGCTAACTAG TACAGACGCA GGCGCAAACA TTAGATGCCG GCACAAACAC
            901 GCACAAAGAC AGAGACAGAC ACGAAAACGA CCGCTGGCCA GCTTACCTCC CGGCGGGGGG
            961 GGTCGGTGGT CCCTGGGGCA GGGGTCTCCA AATCTCGGGT GGAACCTCCA AATGAAAAGA
           1021 ACCCCCGAAG GTGGGCAGTC AATCAAATCT GGAGAGAACC CTCCCAAGGA ATCAGCGAGT
           1081 CAACGAATCG GATGCAACCA GCCAAGAAAG CTTATGGAAT ACGGGTAACC GGCCGAACTC
          1141 AAGTCAACTC CGAAGGAACC TTTG
          //
          Primer: EGFP-C
          Sequence file: RDB12177_A6Ajf.seq check
          >12177_12177_A6Aj_EGFP-C_G09_21.ab1
              1 CAGGCACTCT CGGGCATGGA CGAGCTGTAC AAGTAAAGCG GCCGGCCGCC AGCACAGTGG
             61 TCGACCGGGC CGCCAGCACA GTGGTCGATC GAGGGGGGGC CCGCGATTAG TCCAATTTGT
            121 TAAAGACAGG ATATCAGTGG TCCAGGCTCT AGTTTTGACT CAACAATATC ACCAGCTGAA
            181 GCCTATAGAG TACGAGCCAT AGATAGAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG
            241 GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA
            301 GGCATGGAAA ATACATAACT GAGAATAGAG AAGTTCAGAT CAAGGTTAGG AACAGAGAGA
            361 CAGCAGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC
            421 CAAGAACAGT TGGAACAGCA GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT
            481 GCCCCGGCTC AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC
            541 TAGAGAACCA TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT
            601 TTGAACTAAC CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC
            661 AATAAAAGAG CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC
            721 GGGTACCCGT GTATCCAAAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG GTCTCGCTGT
            781 TCCTTGGGAG GGGTCTCCTC TGAGTGATTG ACTACCCGTC ACGGGGGTCT TACACATGCT
            841 GCAGTATCAA AATTAAATTG GGTTTTTCTT CTTAACTGTA AACATTACAT GAGCAAAATT
          901 GGCATAC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl