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pMYs-HA-Yap1-IRES-EGFP (#RDB12177)

Retroviral vector of mouse Yap1

Clone info. Retroviral vector of mouse Yap1. cDNA fragment 210 to 1,625 nt of NM_009534.3 (CDS, 207..1625) is cloned.
Comment Transfection can be confirmed by GFP. See Fig. 3A of Ota and Sasaki, Development 135: 4059-4069 (2008). Known differences: G to C substitution at 212 nt (Glu to Asp at 2nd aa). C to A substitution at 477 nt (Arg at 91st aa, synonymous) and XhoI site insertion at the position.
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r
Gene/insert name Mouse Yap1 cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Additional terms and conditions:
Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infections virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
MTAに書く付加的使用条件：
pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12177 pMYs-HA-Yap1-IRES-EGFP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMYs-HA-Yap1-IRES-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12177).

Reference section:

Ota, M., Sasaki, H., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19901_B3Bap1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: pol region,insert 5'(HA,Yap1 5')
Sequence file: RDB19901_B3Baa.seq check
>12177_19901_B3Ba_3_MMLV-pol_F_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTCGNANC TTGGNNACAC GCCGCCCACG TGNAGGCTGC
   61 CGACCCCGGG GGTNGNNCAT CCTCTAGACT GCCGGATCTA GCTAGTTAAT TAAGGATCCC
  121 AGTGTGGTGG TACGGGAATT CCTGCAGGGG GCTAGCGAAT TCGATTAATA CGACTCACTA
  181 TAGGGAGACC CAAGCTTGCC GCCACCATGT ACCCATACGA CGTTCCAGAT TACGCTGGTA
  241 CCGACCCCGC GCAACAGCCG CCGCCCCAGC CGGCCCCGCA AGGCCCCGCG CCGCCGTCCG
  301 TGTCTCCGGC CGGGACCCCC GCGGCCCCGC CCGCACCCCC GGCCGGCCAC CAGGTCGTGC
  361 ACGTCCGCGG GGACTCGGAG ACCGACTTGG AGGCGCTCTT CAATGCCGTC ATGAACCCCA
  421 AGACGGCCAA CGTGCCTCAG ACCGTGCCCA TGCGGCTTCG CAAGCTGCCC GACTCCTTCT
  481 TCAAGCCGCC TGAGCCCAAG TCCCACTCGA GACAGGCCAG TACTGATGCA GGTACTGCGG
  541 GAGCTCTGAC TCCACAGCAT GTTCGAGCTC ACTCCTCTCC AGCCTCCCTG CAGCTGGGTG
  601 CCGTTTCTCC TGGGACACTC ACAGCCAGTG GCGTTGTCTC TGGCCCTGCC GCTGCCCCTG
  661 CAGCTCAGCA TCTCCGGCAG TCCTCCTTTG AGATCCCTGA TGATGTACCA CTGCCAGCAN
  721 GCTGGGAGAT GGCCAAGACA TCTTCTGGTC AAAGATACTT CTTAAATCAC AACGATCAGA
  781 CAACNACNTG GNANGANCCC NNGAAGGNCA TGCTTTCGCA ACTGAACGTT CCTGCGCNNG
  841 CCANNCCAGC GGNGCCCCNN ANNCTGATGA NTTCTGCCNN NNNNANCTCT TNCNGATGNA
901 TGGNNNCNNG CCNTNNNTNN NNNNNNNN
//
Primer: Yap1-B_R (Pr1299)
Region: insert 3'(Yap1 3'),IRES
Sequence file: RDB19901_B3Bab.seq check
>12177_19901_B3Ba_3_Yap1-B_R_D02_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NAGTTCCGAN CCCTTTCTTA ACNGTGGCAC
   61 CTATCACTCT CGANANGANA GCACAGACAG CGGCCTCAGC ATGAGCAGCT ACAGCATCCC
  121 TCGGACCCCA GACGACTTCC TCAACAGTGT GGATGAAATG GATACAGGAG ACACCATCAG
  181 CCAAAGCACC CTGCCGTCAC AGCAGAGCCG CTTCCCCGAC TACCTGGAAG CCCTCCCTGG
  241 GACAAATGTG GACCTTGGCA CACTGGAAGG AGATGCAATG AACATAGAAG GGGAGGAGCT
  301 GATGCCCAGT CTGCAGGAAG CGCTGAGTTC CGAAATCTTG GACGTGGAGT CTGTGTTGGC
  361 TGCCACCAAG CTAGATAAAG AAAGCTTTCT CACGTGGTTA TAGAGCTGCA GGGAGCCACT
  421 CTGAGTCTGT GAGGGATCCA CTAGTAACGG CCGCCAGTGT GCTGGAATTC TGCAGATCCT
  481 CGAGGGCCGG CGCGCCGCGG CCGCTACGTA AATTCCGCCC CCCCCCCCCC TCTCCCTCCC
  541 CCCCCCNNNN NNNTNNTGGC CNANNNNGCT TGGAATAAGG CCGGNGNGCN NNNGNCTATA
  601 TGNNNTTTTC NNNCNTATTN NNNNNNTTTG NNNNNGNNNN NNCCCGGAAA CCTGGCCCTG
  661 TCTTCTTGAC NANNATTCCT ANGGGTCTTT NNCCNNCTCN CCAAAGGAAT GCAAGGNCTG
721 NTTGAATGTC NNNAAGGANN CANTNNNNNN NNGAANNNN
//
Primer: IRES-F (Pr0036)
Region: IRES,EGFP 5'
Sequence file: RDB19901_B3Bac.seq check
>12177_19901_B3Ba_3_IRES-F_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCGA NGNNNNNNNA ACGTCTAGGC CCCCCGAACC
   61 ACGGGGNCGT GGNNNTTCCT TTGAAAAACA CGATGATAAT ATGGCCACAA CCATGGTGAG
  121 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
  181 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT
  241 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
  301 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA
  361 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA
  421 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
  481 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA
  541 GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA ACGGCATCAA
  601 GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG CCGACCACTA
  661 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAG
  721 CACCCAGTCC GCCCTGAGCN AAGANCCCNA CGAGAAGCGC GATCACATGG TCCTGCTGGA
  781 NTTCGTGACC GCCGCCGGGA TCACTCTCGG CNTGGACNAN CTGTACAAGT AAAGCGGNCG
  841 GNCGCCAGCA CNNTGGTCGA NCNGGGNCGC CNGCACAGNG GNCGATCGAG GGGGGGCCCN
  901 CGANTANNCN NATTTNNTNA AAGANNNGNA TATCANNGGT CCNNNCTCTN NTTTTGANTC
961 NNNNNTANCN NNNNNNNNN
//
Primer: Amp_R (Pr0008)
Region: AmpR pro,5' LTR,
Sequence file: RDB19901_B3Bad.seq check
>12177_19901_B3Ba_3_Amp_R_H01_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN TTGNNNNNNT TATNNGGGTT ATTGTCTCAT GAGCGGANAC
   61 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA
  121 GTGCCACCTG CATAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC
  181 CATTTTGCAA GGCATGGAAA AATACATAAC TGAGAATAGA AAAGTTCAGA TCAAGGTCAG
  241 GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC AGTTCCTGCC
  301 CCGGCTCAGG GCCAAGAACA GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG
  361 TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA GAACAGATGG TCCCCAGATG CGGTCCAGCC
  421 CTCAGCAGTT TCTAGAGAAC CATCAGATGT TTCCAGGGTG CCCCAAGGAC CTGAAATGAC
  481 CCTGTGCCTT ATTTGAACTA ACCAATCAGT TCGCTTCTCG CTTCTGTTCG CGCGCTTCTG
  541 CTCCCCGAGC TCAATAAAAG AGCCCACAAC CCCTCACTCG GCGCGCCAGT CCTCCGATTG
  601 ACTGAGTCGC CCGGGTACCC GTATTCCCAA TAAAGCCTCT TGCTGTTTGC ATCCGAATCG
  661 TGNACTCGCT GATCCTTGGN AGGGTCTCCT CAGATTGATT GACTGCCCAC CTCGGGGGTC
  721 TTTCATTTGG AGGTTCCACC GAGANTTGGA GANCCCTGCC CAGGGACCNN CGACCCCCCC
  781 GCNNGGAGGT AAGCTGGNCA GCGGNCGTTT CGTGTCTGTC TCTGTCTTTG TGCGTGNTTG
  841 TGCCGGCNTC NNANGNTTGC NCCTGCGTCT GNNCTANTTA GCNNNCTNNC TCTGTNNCTG
  901 GCGNANCNNN GGNNGANNTN NNNANTNNNN ANNNNNNNNC GNNNCNNGGN ANANNTNCCN
961 NGNNNNTCNG GGNNNNTTTT NNNNNNNNNA NNTNNNTNNA ANNNNNNNNN N
//
Primer: pMFG_1 (Pr0230)
Region: pol region,psi
Sequence file: RDB19901_B3Bae.seq check
>12177_19901_B3Ba_3_pMFG_1_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGG CNNTNNNNNA CTCTTGTNNT GTNNGGGTCA GGGNAGTTTA
   61 CNAGGGGCGG GGNNNNNCCC NNTATAAGAT CTCATATGGC CATATGGGGG CGCCTAGAGA
  121 AGGAGTGAGG GCTGGATAAA GGGAGGATCG AGGCGGGGTC GAACGAGGAG GTTCAAGGGG
  181 GAGAGACGGG GCGGATGGAG GAAGAGGAGG CGGAGGCTTA GGGTGTACAA AGGGCTTGAC
  241 CCAGGGAGGG GGGTCAAAAG CCAAGGCTTC CCAGGTCACG ATGTAGGGGA CCTGGTCTGG
  301 GTGTCCATGC GGGCCAGGTG AAAAGACCTT GATCTTAACC TGGGTGATGA GGTCTCGGTT
  361 AAAGGTGCCG TCTCGCGGCC ATCCGACGTT AAAGGTTGGC CATTCTGCAG AGCAGAAGGT
  421 AACCCAACGT CTCTTCTTGA CATCTACCGA CTGGTTGTGA GCGATCCGCT CGACATCTTT
  481 CCAGTGACCT AAGGTCAAAC TTAAGGGAGT GGTAACAGTC TGGCCCTAAT TTTCAGACAA
  541 ATACAGAAAC ACAGTCAGAC AGAGACAACA CAGAACGATG CTGCAGCAGA CAAGACGCGC
  601 GGCGCGGCTT CGGTCCCAAA CCGAAAGCAA AAATTCAGAC GGAGGCGGGA ACTGTTTTAG
  661 GTTCTCGTCT CCTACCAGAA CCACATATCC CTCCTATTAG GGGGGTGCAN CAAAGAGTCC
  721 AAAACGATCG GGATTTTTGG ACTCANGTCG GGCCACAAAA AACGGCCCCC GAAGTCCCTG
  781 NNACGTCTCC CAGGGTTGCG GNCNGGNGTT CCNAACTCGT CAGTTCCACC NCGGGTCCGC
  841 CAGATACAGA NCTAGTTAGC TANCTAGTAC AGACGCNNNN NCAAACATNA NNNGCCGGCA
  901 CAAACACGCN CAAAGNNNGA NACAGANNNN AAACGNNNGC NNGNNNNNCT NNNNTNCNNN
961 NNGGGGGGGT CNGNNGGNNN CCNNNGNNNN GNNNNNNNNN ANNNNNNNNN N
//
Primer: mVenus-M_F (Pr0574)
Region: EGFP 3',3' LTR
Sequence file: RDB19901_B3Baf.seq check
>12177_19901_B3Ba_3_mVenus-M_F_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTCNNGGT GANTTCNAGA NCCGCCACAA CATCGAGGNN
   61 GGCAGCGTGC AGCTCGCCNA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG
  121 CTGCTGCCCG ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG
  181 AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG
  241 GACGAGCTGT ACAAGTAAAG CGGCCGGCCG CCAGCACAGT GGTCGACCGG GCCGCCAGCA
  301 CAGTGGTCGA TCGAGGGGGG GCCCGCGATT AGTCCAATTT GTTAAAGACA GGATATCAGT
  361 GGTCCAGGCT CTAGTTTTGA CTCAACAATA TCACCAGCTG AAGCCTATAG AGTACGAGCC
  421 ATAGATAGAA TAAAAGATTT TATTTAGTCT CCAGAAAAAG GGGGGAATGA AAGACCCCAC
  481 CTGTAGGTTT GGCAAGCTAG CTTAAGTAAC GCCATTTTGC AAGGCATGGA AAATACATAA
  541 CTGAGAATAG AGAAGTTCAG ATCAAGGTTA GGAACAGAGA GACAGCAGAA TATGGGCCAA
  601 ACAGGATATC TGTGNTAAGC AGTTCCTGCN NCGGCTCANG GCCNAGAACA GTTGNAACAG
  661 CAGANTATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTN CNTGCCCCNG NTCNNGNCCN
  721 ANAACAGATG GTCCCCANAN GCGGNCCCGC CCTCNNNANT TTCTAGAGAA NCNTCANANG
781 TTTCCNGGGN GNCCNNNNNN NCNNAAATGA NCCNNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development, 135, 4059-4069 (2008). PMID 19004856. [link to RRC of NBRP]