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pcDNA3.1-pA83-Lats2KD (#RDB12201)

Expression clone of mouse Lats2 cDNA, kinase dead variant

Clone info. Expression clone of mouse Lats2 cDNA. Catalytically inactive (kinase dead) variant. N-terminal HA-tag.
Comment D767A mutation in kinase subdomain VII, corresponding human D809A mutation. The expression vector has a stretch of poly(A).
Vector backbone pcDNA3.1-pA83 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Lats2 cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Nishioka, N. et al., Dev. Cell 16, 398-410 (2009))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12201 pcDNA3.1-pA83-Lats2KD DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-pA83-Lats2KD was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12201).

Reference section:

Nishioka, N., Inoue, K., Adachi, K., Kiyonari, H., Ota, M., Ralston, A., Yabuta, N., Hirahara, S., Stephenson, R.O., Ogonuki, N., Makita, R., Kurihara, H., Morin-Kensicki, E.M., Nojima, H., Rossant, J., Nakao, K., Niwa, H., Sasaki, H., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12201_A4Fup1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: HA tag, insert 5'
Sequence file: RDB12201_A4Fua.seq check
>12201_12201_A4Fu_CMV-Foward_A09_03.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNANNTGGG NGGNANGCGT GTACGGTGGG
   61 AGGTCTATAT AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG
  121 AAATTAATAC GACTCACTAT AGGGAGACCC ANNNNNNNTA GTTAAGCTTG GTACCGAGCT
  181 CGGATCCACT AGTCCAGTGT GGTGGAATTG ATCCCAGTGT GGTGGTACGG GAATTCCTGC
  241 AGGATGTACC CATACGACGT TCCAGATTAC GCTGGTACCA TGAGGCCAAA GACTTTTCCT
  301 GCCACAACTT ACTCTGGAAA TAGCCGGCAG CGATTGCAAG AGATTCGAGA GGGGCTGAAG
  361 CAGCCATCCA AGGCTTCCAC CCAGGGGCTG CTGGTGGGAC CAAACAGTGA CACTTCCCTG
  421 GATGCCAAAG TCCTGGGGAG CAAAGATGCC TCCAGGCAGC AGCAAATGAG AGCCACCCCG
  481 AAGTTTGGAC CTTATCAAAA AGCTCTCAGG GAAATCCGAT ATTCCCTCCT GCCTTTTGCC
  541 AACGAGTCAG GCACTTCGGC AGCTGCAGAG GTGAACCGGC AGATGCTTCA GGAGTTGGTG
  601 AATGCGGGAT GTGACCAGGA GATGGCTGGC AGAGCGNNNN NNCAGACGGG CAGTAGGAGT
  661 ATCGAAGCTG CCTTGGAGTA CATCAGTAAG ATGGGCTACC TGGACCCCAG GAATGAGCAG
  721 ATTGTGCGAG TCATCAAGCA GANCTCCCCA GGAAAAGGGN CTGGCGCCCA CCCCGGTGAC
  781 TCNGCGGCCC ANTTTCGAGG GCACAGGGGA AGCACTCCCA TCCTACCNNN AGCTGGGTGG
  841 TGCAAACTAC NANGGCCCCG CCGCANTGGA NGANATGCCG CGGCANTATT TNAGACTTTN
  901 NCNTCCCTGN NNCCGGAGCC NGGCANCNNN NGNNGCCCAG NCTCNNNAGC ANNNNNCNNA
  961 ANNGGNNNNA GCACANNANN AGANNCNANN GCGNNANTTT NNNNGGNCNN NNCNCNNNNN
1021 GNTNNNNNNN NNN
//
Primer: GNP Primer #10 (Pr0763)
Region: TRF2-3'UTR+polyA83, insert3'
Sequence file: RDB12201_A4Fub.seq check
>12201_12201_A4Fu_GNP_primer#10_B09_06.ab1
    1 NNNNNNNNNN NNNNATTTNN ANNNNAACGT TTACNGGTTC TTTGGTGCCC ATAGTCGAGC
   61 GGCCGCCACT GTGCTGGATA TCTGCAGAAT TTCGAGGTTA CACGTACACC GGCTGGCAGC
  121 CCTCGGCCGC ACCTTCCAAG TCCGCATCCC CTGGGTCTGC ACTCTCTGCG GGCTCTGAGG
  181 GCTTCGGGCA CCGGAAGGGA TAGCCATTGT CATCGAAGAA CCTGCGGAAG GTGAACTCAT
  241 AGAAGGCGTG CTCTGGATGC TTGCTGCTGG GGGAGGCCAG CGTGTCCCAG GCCTTGGCGC
  301 TCTCTCCGCT GGCCTCGTGC CAGGGGCTTT CTTCATCCAC CGGGTCAAAA TTGGAGGTGT
  361 CCATGGGGTG GCTGATGGTG GGGACGTAGG GTGCAGGCTG CTTTCGGATG TCACGGGAAA
  421 AGTCGATGGT GTTGAAGAAC GGGTGTGCCT TGAGGTCATC TGCCCCATCC CTGCCCAGGC
  481 GGCAGTCAGC CGCGCAGCAC AGCTTCGTGA TGAGGTCTCG GGCCTCAGCG CTGANCCTCA
  541 CCTGCGTANG GATATGCAGC GTGCTCTCCC AGTTGATCAC CTTCAGCTGC GTCTCTGTGG
  601 GGGTGGGGGN CAAGAAAGGN GGNTGNCNNA CCNGCATCTC NAAGAGAATC ACACCGACNN
  661 TNCNCCNNTC NNANANNTGC NNNNANCNTT NGNNNANAAN CANNNNCNNA NCGANGTNNT
721 TNNNNNNNCC NANNNNANAN TGNNNNNNNN NN
//
Primer: Lats2#11 (Pr0039)
Region: insert mid
Sequence file: RDB12201_A4Fuc.seq check
>12201_12201_A4Fu_Lats2#11_C02_08.ab1
    1 NNNNNNNNNN CNNNNNGCNN NNGCTGGNNC AGGNANNGGC CNAAGCTGGG CTCTGTGAGG
   61 CCGAGCAGGA GCAGATGAGG AAGATCCTCT ACCAGAAGGA GTCTAACTAC AACCGGCTGA
  121 AGAGGGCCAA GATGGACAAG TCCATGTTTG TGAAAATCAA GACTCTAGGC ATCGGTGCCT
  181 TTGGGGAAGT GTGCCTCGCT TGTAAGCTGG ACACTCACGC TCTGTACGCC ATGAAGACTC
  241 TCAGGAAGAA GGATGTCCTG AACCGGAATC AAGTGGCCCA TGTCAAGGCT GAGAGGGACA
  301 TCCTGGCTGA AGCAGACAAT GAGTGGGTGG TCAAACTCTA CTACTCCTTC CAGGACAAGG
  361 ACAGCCTGTA CTTTGTGATG GACTACATAC CAGGCGGGGA TATGATGAGC CTGCTGATCA
  421 GGATGGAGGT CTTCCCTGAG CACCTGGCCC GCTTCTACAT TGCAGAGTTG ACCCTGGCCA
  481 TTGAAAGTGT CCACAAGATG GGCTTTATCC ACCGGGACAT CAAGCCTGAC AACATACTCA
  541 TCGACCTGGA TGGTCATATT AAGCTGACAG CTTTTGGCCT CTGCACTGGA TTCAGGTGGA
  601 CTCACAATTC CAAGTACTAC CAGAAAGGGA ACCACATGAG ACAGGACAGC ATGGAGCCCG
  661 GTGACCTCTG GGACGATGTT TCCAACTGTC GCTGTGGAGA CAGGTTAAAG ACCCTGGAGC
  721 AGANGGCGCA GAAGCAGCAC CAGANGTGCC TGGCACATTC TCTTGTCGGG ACACCAAATT
  781 ACATCGCTCC NGANGTGCTT CTCCGCNAAG GGTACACGCA GCTCTGTGAC TGGTGGANCG
  841 TCGGTGTGAN TCTCTTTGAN ATGCTGGTTG GGCAGCCGCC TTTCNNGGCC NNCACCCCCN
  901 NCAGANNNNC ANCTGNNNNN TGATCAACTG GNNNANCNNN CNNNNNNTCC NNACNNNNGN
961 NANGNNCANC GNNNNNNNCN NNNNNNNCAT CNCNAANNNN NNNNNN
//
Primer: BGH_rev2 (Pr0606)
Region: TRF2-3'UTR_polyA83
Sequence file: RDB12201_A4Fud.seq check
>12201_12201_B0CpF_BGH_rev2_C04_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGGNGGNNG GGCNAACNAC AGATGGCTGG CAACTAGAAG
   61 GCACAGTCGA GGCTGATCNN CGGGTTTAAA CTCAATGGTG ATGGTGATGA TGACCGGTAT
  121 GCATATTCAG ATCCTCTTCT GAGATGAGTT TTTGTTCGAA GGGCCCTCTA GACTCGAGGT
  181 ACGCCACAGC GTATGATGCG TATTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
241 TTTTTTTTTT TTTTTTTTTT TTTTNNNNNN NNNN
//
Primer: SV40pro_F (Pr0110)
Region: SV40 pro_ori,NeoR
Sequence file: RDB12201_A4Fue.seq check
>12201_12201_A4Fu_SV40pro_F_G10_19.ab1
    1 CCCTGTCCTG GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
  121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
  661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  781 AGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
  841 CTTCCTCGTG CTTTACGGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  901 TTCTTGACGA GTTCTTTCTG AGCGGGACTC TGGGGTTTCG CGAAATGACC CGAACCCAAG
  961 CGAACGCCCA TCCTGCCCAT CACGAGATTT CCGATTCGAT CGGCCGCCGT TCTTATGAAA
1021 TGTTCGGCTT CGTATTCGAT TTTCCAGGAA CGATC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nishioka, N., The Hippo signaling pathway components Lats and Yap pattern Tead4 activity to distinguish mouse trophectoderm from inner cell mass. Dev. Cell, 16 (3): 398-410 (2009). PMID 19289085. [link to RRC of NBRP]