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HA-Amot130/pcDNA3.1-pA83 (#RDB12203)

Expression clone of mouse Amot (p130)

Clone info. Expression clone of mouse Amot (p130). HA-tag.
Comment See Fig. 4A of Hirate et al., Curr. Biol. 23: 1181-1194 (2013). The expression vector has a stretch of poly(A).
Vector backbone pcDNA3.1-pA83 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Amot cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12203 HA-Amot130/pcDNA3.1-pA83 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The HA-Amot130/pcDNA3.1-pA83 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12203).

Reference section:

Hirate, Y., Hirahara, S,. Inoue, K., Suzuki, A., Alarcon, V.B., Akimoto, K., Hirai, T., Hara, T., Adachi, M., Chida, K., Ohno, S., Marikawa, Y., Nakao, K., Shimono, A., Sasaki, H., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12203_A5Hpp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: HA tag, insert 5'
Sequence file: RDB12203_A5Hpa.seq check
>12203_12203_A5Hp_CMV-Forward_A04_01.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGCNNNGG GCGGTAGGCG TGTNCNGNGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGTTAANCTN NCTTGCCGCC ACCATGTACC
  181 CATACGACGT TCCAGATTAC GCTGGATCCA GAAGTTCTGA CGATCAGCCA AGTGGAGGAA
  241 CGACGGTACT GCAACGGCTG CTTCAAGAGC AGCTTCGCTA TGGCAATCCC AGTGAGAATC
  301 GCAGTCTGCT TGCCATACAC CAGCAAGCCA CAGGGAATAG CTCTCCATTT TCCACGGGCA
  361 GTGGGAACCA AGGCCCTCAG AATGATGTAT TGAGTTCCCA GGATCACCAC CAGCAGCAGC
  421 TTGTGGCACA CCCTGCTCGC CAAGAGCCCC AGGGTCAGGA AATCCAGTCA GAAAACGGCG
  481 TCATGGAGAA GCAGCTGTCC CCTCGAATGC AGAATAACGA AGAACTCCCA ACCTATGAAG
  541 AGGCCAAGGT CCAGTCCCAG TACTTCAGGG GCCAGCAGCA TGCTAGTGTT GGGGCTGCTT
  601 TCTATGTCAC AGGAGTCACC AACCAGAAGA TGAGAACCGA GGGACGCCCG TCAGTCCAGC
  661 GCCTCACGCN CGGGAAGATG CACNAAGATG AAGGNCTTCG AGATCTTAAG CAAGGGCATG
  721 TCCGATCCTT GAGTGAAAGA CTGATGCAGA TGTCATTGGC TACTAGTGGA GTTAAAGCCC
  781 ACCNNCCTGT TANCAGTGCT CCTCTCTCCN CACCACAACC CNATGATCTC TACAAGAATG
  841 CCACCAGTTN NNGTGAATTC TACAAGGNCN NAGGGCCANN NNNNAGNNNA CNTAGNCTGA
  901 AGGGNNTGGA ANNNNNAGGN CCCNNGCCAG ANTANCNTTT CAAGGGCGTG NNNTCNNNNN
  961 NNNNAGTGTG NNAGTCCNNN NANNNNGGNN NNNNCNNNAN TGANCNTNNN CNTNNNNNNG
1021 NCNNNNNNNN NN
//
Primer: mAmot-M#1
Sequence file: RDB12203_A5Hpb.seq check
>12203_12203_A5Hp_mAmot-M#1_E08_14.ab1
    1 AGGGGAGAGC TACTCAGCTA TGCCTCGGGC TCAGCAGTCT GCTTCTTATC AGCCCATGCC
   61 AGCTGACCCT TTTGCTATCG TCTCTAGAGC TCAGCAGATG GTTGAGATCC TGTCTGATGA
  121 GAATCGCAAC TTGCGGCAGG AGTTGGATGG ATGCTATGAG AAGGTGGCGA GACTGCAGAA
  181 GGTGGAGACA GAAATCCAAC GGGTCTCAGA GGCTTATGAG AACTTGGTGA AGTCATCTTC
  241 CAAAAGAGAG GCTCTGGAGA AAGCCATGAG GAACAAGCTG GAGGGCGAGA TTAGAAGGAT
  301 GCATGACTTC AACAGAGATC TGAGAGACCG TCTAGAGACT GCCAACAAGC AGCTGGCAGA
  361 GAAGGAGTAC GAGGGGTCCG AGGACACCAG GAAGACCATC TCGCAGCTCT TTGCCAAACA
  421 TAAAGAAAAC CAGCGGGAGA AGGAGAAGCT GGAAGCTGAG CTGGCTACTG CCCGTTCTAC
  481 CAATGAAGAC CAAAGGCGAC ACATTGAAAT CCGAGACCAG GCATTGAGCA ATGCCCAGGC
  541 GAAGGTGGTG AAGCTGGAAG AAGAGCTGAA AAAGAAGCAA GTGTACGTAG ATAAGGTGGA
  601 GAAGATGCAG CAGGCCCTTG TGCAGCTCCA GGCCGCCTGT GAGAAGCGTG AGCAGCTGGA
  661 ACACCGGCTC CGGACACGTT TGGAGAGGGA ACTGGAATCC CTTAGAATCC AGCAGCGCCA
  721 GGGCAACTCC CAGCCCACTA ATGCTTCAGA ATACAATGCC GCTGCACTGA TGGAGCTCCT
  781 CCGGGAGAAG GAAGAGAGAA TTCTGGCCCT GGAAGCAGAT ATGACCAAGT GGGAGCAAAA
  841 GTATTTGGAA GAGAATGTGA TGAGACACTT TGCTCTGGAT GCTGCTGCAA CTGTGGCTGC
  901 ACAGAGAGAC ACAACAGTCA TTAGCCACTC TCCTAACACC AGCTATGACA CAGCCCTTAG
  961 AGGCCCGCAT CCAGAAGGAA GAGGAGAAAT CCTGATGGGC CAACAAGCGT TGCTTGACAT
 1021 GGAAGGAGGA TTAAGACCTC ATGCCAGATA TGAAAAGATG CAATGATCAG TGCTGCAGCA
 1081 GCGCTCCAGG AAAGAAGCGA GTTAAACCAG AGCAACTTCG ATCCAATGCG CAGTCAGTCC
1141 TGAATGTTCC GATCTTTTTT CA
//
Primer: mAmot-M#1 (Pr0282)
Region: insert mid
Sequence file: RDB12203_A5Hpc.seq check
>12203_12203_A5Hp_mAmot-M#1_E08_14.ab1
    1 NNNNNNNNNN NNNNNNGCNN TGCCTCNGGC TNNGCAGTCT GCTTCTTATC NGCCCNTGCC
   61 NGCTGACCCT TTTGCTATCG TCTCTAGAGC TCAGCAGATG GTTGAGATCC TGTCTGATGA
  121 GAATCGCAAC TTGCGGCAGG AGTTGGATGG ATGCTATGAG AAGGTGGCGA GACTGCAGAA
  181 GGTGGAGACA GAAATCCAAC GGGTCTCAGA GGCTTATGAG AACTTGGTGA AGTCATCTTC
  241 CAAAAGAGAG GCTCTGGAGA AAGCCATGAG GAACAAGCTG GAGGGCGAGA TTAGAAGGAT
  301 GCATGACTTC AACAGAGATC TGAGAGACCG TCTAGAGACT GCCAACAAGC AGCTGGCAGA
  361 GAAGGAGTAC GAGGGGTCCG AGGACACCAG GAAGACCATC TCGCAGCTCT TTGCCAAACA
  421 TAAAGAAAAC CAGCGGGAGA AGGAGAAGCT GGAAGCTGAG CTGGCTACTG CCCGTTCTAC
  481 CAATGAAGAC CAAAGGCGAC ACATTGAAAT CCGAGACCAG GCATTGAGCA ATGCCCAGGC
  541 GAAGGTGGTG AAGCTGGAAG AAGAGCTGAA AAAGAAGCAA GTGTACGTAG ATAAGGTGGA
  601 GAAGATGCAG CAGGCCCTTG TGCAGCTCCA GGCCGCCTGT GAGAAGCGTG AGCAGCTGGA
  661 ACACCGGCTC CGGACACGTT TGGAGAGGGA ACTGGAATCC CTTAGAATCC AGCAGCGCCA
  721 GGGCAACTCC CAGCCCACTA ATGCTTCAGA ATACAATGNC GCTGCACTGA TGGAGCTCCT
  781 CCGGGAGAAN GAAGAGAGAA TTCTGGCCNT GGAAGCAGAT ATGACCNAGT GGGANCAAAA
  841 GTATTTGNNA GAGANTGTGA TGAGANACTT TGCTCTGGNN GCTGCTGCAN NNGNNGCNGC
  901 ACAGAGAGAC ACNACAGNCA NNANCNNNTN NNNTAACANN AGNNATGNNN CAGNCNNTAG
961 NNNNCNNNNT NCNGNNNNNN NNNN
//
Primer: mAmot-M#2 (Pr0283)
Region: insert mid
Sequence file: RDB12203_A5Hpd.seq check
>12203_12203_A5Hp_mAmot-M#2_H10_22.ab1
    1 NNNNNNNNNN NNNNNNNCNN NNNNNNNGNN TAGCTGANTA GGCTTCTCCT GGCTGCTGCT
   61 GCGCTGAAGT CAGGCTGGCC TGGGCGTGAC GGTAGTGGTC CCCCTGATGA CTCTGACTCG
  121 AAAGAAGATG CTGCTGATGC CTTGACAGGT GAGCAGAGTG GTCACCTTGG TTCGGGACCA
  181 GGTGTTGACC AGGAGGCAAT CTAGTGGACG GCGGAGACTG CAGCAAGGGC AAGCTGGAAG
  241 CCCCAGCTGT CAGGGAAGAA GTAGGGCTAT GAGGTTGAGA GTTCCTGGCT GACAAAGACA
  301 GCGAGGAGAT GTCCTGTGTT GCCCTGGCTG CCCCATACTC AGGGGGGTGC TGATACCTCA
  361 TCAGTTGCCC CTCTGTTCTC CCTGGCTGGT TCAGGCGATG CTCACTATAG AAGTGCCCTG
  421 GCTCTTGGGA CTTACACACT ACAGACTGGG ATGGCACGCC CTTGAAAGGG TATTCTGGCG
  481 GGGGACCTCG GTGTTCCATG CCCTTCAGAC TATGTTGGCT TGGAGGTGGC CCTTGGGCCT
  541 TGTAGAATTC ACTGGAACTG GTGGCATTCT TGTAGAGATC ATTGGGTTGT GGTGGGGAGA
  601 GAGGAGCACT GGTAACAGGT GGGTGGGCTT TAACTCCACT AGTAGCCAAT GACATCTGCA
  661 TCAGTCTTTC ACTCAAGGAT CGGACATGCC CTTGCTTAAG ATCTCGAAGG CCTTCATCTT
  721 GGTGCATCTT CCCGGGCGTG AGGCGCTGGA CTGACGGGCG TCCCTCGGTT CTCATCTTCT
  781 GGTTGGTGAC TCCTGTGACA TANAAAGCAG CCCCNACACT AGCATGCTGC TGGNCCCTGA
  841 AGTACTGNGA CTGGACNTTG GCCTCTTCAT ANGTTGGGAN TTCTTCGTTA TTCTGCATTC
  901 NAGGGGACAG CTGCTTCTNC NTGANGCCGT TTTCTGANTG GNTTTCCTGA NCCNNGGGCT
  961 CNTNNNNANC NGGNNGNGNN NCAAGCTGCT GNTNGNNNNG ATNCNGGNNN NTCNNNACNT
1021 CNTTNNGNNN NNNNNNN
//
Primer: BGH_rev
Sequence file: RDB12203_A5Hpe.seq check
>12203_12203_A5Hp_BGHrev_G08_20.ab1
    1 CACTACTCAT GGTGATGGTG ATGATGACCG GTATGCATAT TCAGATCCTC TTCTGAGATG
   61 AGTTTTTGTT CGAAGGGCCC TCTAGACTCG AGGTACGCCA CAGCGTATGA TGCGTATTTT
  121 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  181 TTTTTTTTTC CTTCCCCAAC TTTTTTTTGG GCCCCTTCCT TTTAAAAAAA AAACCTTTTC
  241 GGGGTTTTTG GGGCCCCAAA CCCAGGGGCC CCTTTTAAAA CCCCTTTCGC TTAACCCTTT
  301 TTTTTGGGGG CCCCCCCAAA ATTCCCAAAA AAAAAGGGAA ACCCTTTCCG GGGGTTTTCC
  361 TTGGGCCCCC CCAAACCCCA AAAAAACCCC CTTTAAAAAA AATCCCCCCC CCCCCCCCCC
  421 CCTTTTTCCC CCCCAATTAA ACAAAGGGAG GGGAAAAATT TTTAAAAAAA ACCCTTTGGA
  481 AAACCCCCCC CCTTGATTGG GGGTTTTAAA AAAAAATTCC CCCTTCCCGG AAAAGGGTTT
  541 TGCCCCAAAA TTTGGAGGGG GGTTTCCTTT CAAAAGGGGG GGGGGGAACC GGGGGCTTCC
  601 GTTATACCTG TTTTGCCTAC ATATGGTGAA ACACCGCGGT CCGGGAGGGG CCCGGATTTG
  661 AGGACCTCGT TTGACCAAGC TGAATTGATT GAACGGACTA GAGTCCTCGA CTGATTATCC
  721 TGATCGATCG AACAACCGGG CTTGGGGGCC GGATCAGCTG GATGACCGGA CCAAGTTCGA
  781 CACTGGATCC CATGGCCACC CCCCCAGTTA GCCAATAACT CGCACAGCAG ATACTTGTCC
  841 GTGGAACTTC AAGGTTTAAT TTCCGCGTAT CGACGGCGTG ACGGACCTTG ACATGTGACG
901 ATCCTGTAAT ATTCGAAAAA
//
Primer: SV40pro_F
Sequence file: RDB12203_A5Hpf.seq check
>12203_12203_A5Hp_SV40pro_F_D04_10.ab1
    1 GCGCTTGGTC ATTAACATTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC
   61 AAAAAGCTCC CGGGAGCTTG TATATCCAAT TTCGGATCTG ATCAAGAGAC AGGATGAGGA
  121 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
  181 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
  241 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
  301 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
  361 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
  421 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
  481 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
  541 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
  601 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC
  661 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
  721 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC
  781 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT
  841 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
  901 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGGT TCGCGAAATG ACCGACCAAG
  961 CGACGCCCAA CCCTGCCATC ACGAGATTTC GATTCCCACC GCCGGCCTTC TATGAAAGGT
 1021 TGGGCGTCGG AAATCGTTTT CCGGGGACGC CGGGCTGGAA TGGATTCGTC CAGCGACGGG
1081 GGAATCTCAA TGGCGTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]