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HA-Amot80/pcDNA3.1-pA83 (#RDB12204)

Expression clone of mouse Amot (p80)

Clone info. Expression clone of mouse Amot (p80). HA-tag.
Comment See Fig. 4A of Hirate et al., Curr. Biol. 23: 1181-1194 (2013). The expression vector has a stretch of poly(A).
Vector backbone pcDNA3.1-pA83 (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name Mouse Amot cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12204 HA-Amot80/pcDNA3.1-pA83 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The HA-Amot80/pcDNA3.1-pA83 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12204).

Reference section:

Hirate, Y., Hirahara, S,. Inoue, K., Suzuki, A., Alarcon, V.B., Akimoto, K., Hirai, T., Hara, T., Adachi, M., Chida, K., Ohno, S., Marikawa, Y., Nakao, K., Shimono, A., Sasaki, H., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12204_A5Hpp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB12204_A5Hpa.seq check
>12204_12204_A5Hp_CMV-Forward_E11_14.ab1
    1 TCGTTTCAAC TTCCGCCCCA TTTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
   61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
  121 AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGTTAA GCTAGCTTGC CGCCACCATG
  181 TACCCATACG ACGTTCCAGA TTACGCTGGT ACCCCTCGGG CTCAGCAGTC TGCTTCTTAT
  241 CAGCCCATGC CAGCTGACCC TTTTGCTATC GTCTCTAGAG CTCAGCAGAT GGTTGAGATC
  301 CTGTCTGATG AGAATCGCAA CTTGCGGCAG GAGTTGGATG GATGCTATGA GAAGGTGGCG
  361 AGACTGCAGA AGGTGGAGAC AGAAATCCAA CGGGTCTCAG AGGCTTATGA GAACTTGGTG
  421 AAGTCATCTT CCAAAAGAGA GGCTCTGGAG AAAGCCATGA GGAACAAGCT GGAGGGCGAG
  481 ATTAGAAGGA TGCATGACTT CAACAGAGAT CTGAGAGACC GTCTAGAGAC TGCCAACAAG
  541 CAGCTGGCAG AGAAGGAGTA CGAGGGGTCC GAGGACACCA GGAAGACCAT CTCGCAGCTC
  601 TTTGCCAAAC ATAAAGAAAA CCAGCGGGAG AAGGAGAAGC TGGAAGCTGA GCTGGCTACT
  661 GCCCGTTCTA CCAATGAAGA CCAAAGGCGA CACATTGAAA TCCGAGACCA GGCATTGAGC
  721 AATGCCCAGG CGAAGGTGGT GAAGCTGGAA GAAGAGCTGA AAAAGAAGCA AGTGTACGTA
  781 GATAAAGGTG GAGAAGATGC AGCAGGCCCT TGTGCAGCTC CAGGCCGCCT GTGAGAAGCG
  841 TGAGCAGCTG GAACACCGGC TCCGGACACG TTTGGAGAGG GGAACTGGAA TCCCTTAGAA
  901 TCCAGCAGCG CCAGGGGCAA CTCCCAGCCC ACTAATGCTT CAGATACAAT GCCGCTGCAC
  961 TGATGGAGCT CCTCCGGGAG GAGGGAAGAG AGAATTCTGG CCCTGTAGCA GATATGACCA
 1021 AGTGGGAGCA AAGTATTCGC AGAGATTGTG ATGGAGACAC TTGGCCTCTG GGATGCTGCT
 1081 GCCTACTGTG GCTGCCGAGA GAATCTGAGA CGTCAGTAGT CTACTCCTCT AACACAACCA
1141 GCCCTACTGT CGAACATACG
//
Primer: GNP_primer#10
Sequence file: RDB12204_A5Hpb.seq check
>12204_12204_A5Hp_GNP_primer#10_F04_16.ab1
    1 TTTATTTGTG CAATTCATTT AAAAGATAAC GTTTACAGGT TCTTTGGTGC CCATAGTCGA
   61 GCGGCCAGCT TTAGAGCACA TTCTGCTTAA CCTATCTTTT TGAGGTACTC CCTAAGTATT
  121 CCAAAATAGA TTGGAATTCG TTTCTATGGT ATTACTTATG TACTACCAGA GTCCAATAAA
  181 AAGTCCCTTT AGAAAAATCA TCCCCACCTC CCCACCCTAT CTTCACCCCA GATTAAACAA
  241 ATGAAAGGGA AAAATTATTA AAAAGAACTC TTGCTGATAA CGCCAGCTCT TGATTGTGCT
  301 GTTTAGATGA GATATTCCAC CATCTCTGCA TCAGGCTCTT GCCCCAGGAT CTGAATGGGT
  361 GTCTTTCTTT CCAGAGTGCT GGAGTGGAAA GCAGGGGCAT CTGGCTTATC TGGGTTGCAC
  421 ATCAGATTTG GTGTAGACAA GCGGCGTGGT CCGGAACTGG CACCTGGATT TGCAGGACCC
  481 TCAGTTTGAA CCAAAGCTGG AGTTGGAGTT GGAACTGGAC TAGGAGGTTC AGTCGGAGCT
  541 GGAGTTGATG CCTGAGTCGG AGTCGGAGCA GAAGCCTGAG CCGTTGCTGG AGCCGGAATC
  601 GAAGCTGGAG ATGGAACCGG AGCCGAAGTA GCTGGAGCAA CCTGAATAGC AGTAGTGGCA
  661 GCAGCAGCAG CAGCAGTGAT AGCAGCAGTA GTAGCTGCAG CAACAGCAGC AGTAGCAGTG
  721 GCAGCAGTTG GAGAAACAGT AGCAGGAGTA AGAGATGCAG CAGCTGGAAT CTGAACAGCA
  781 GCAGCTGGAG AGACAGGAGC AGCTGGAGGC ACTACAGCAG CTGGAGCGAC AGGAGCAGCT
  841 GGAGAGATAG AATTAGCTGC AGAGACAGAA GTAGCTGCAG AGACAGAAGT AGCTGGAGCG
  901 GCAGCAGCGG CAAGAGCAGC AGCGTTTGCT GGAGATGGGG TGGCGGCAGC AGCAGCTGGA
  961 GCAGCAACAG CAACTGGAGC AGCAGCTACC ATGATAGTGG TGTTGGTGGC AGCAGTGGCA
 1021 GTGTTGGGTG GCAGCAGTTG CATTGATGGC AGCAGCAGGG CAGCAGCAGC ACTGGACAGC
 1081 CATTGGAGCC GGAGATGGGG AGTGACAGCT GCGGTTTGGG TCGATTTCGT TCCACGTCAG
 1141 TTGGGGTGCC TGCAATCGCG AACTGCCTGT CTTCGGACTG GAGCTGGAGA AGAAAAAGTG
1201 A
//
Primer: BGH_rev
Sequence file: RDB12204_A5Hpc.seq check
>12204_12204_A5Hp_BGH_rev_G04_19.ab1
    1 CGTACTCATG GTGATGGTGA TGATGACCGG TATGCATATT CAGATCCTCT TCTGAGATGA
   61 GTTTTTGTTC GAAGGGCCCT CTAGACTCGA GGTACGCCAC AGCGTATGAT GCGTATTTTT
  121 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  181 TTTTCCCTTT CCAAACTTTT TTTTGGGCCC CTTCAATTTA AAAAAAAAAA TTTTAAGGGG
  241 TTTTTGGGGC CCATAATCAA GGGGCCCCTT TTAAAACCCT TTTTGTTTAA CCCTTTTTTT
  301 TGGGGGCCCC CCCAAATTTC CCAAAAAAAA AGGGAATTTT TTTTTTGGGG TTTTTTTTTG
  361 TCCCCCCAAA ACCCAAAAAA AAGCCCCTTT AAAAAAAACC CCCCCCCCCC CCCCCCCTTT
  421 TTTCCCCCCC AATTAAAAAA AGGGAGGGGA AAAATTTTTA AAAAAACCCT TTGGGAAAAA
  481 CCCCCCCCCT TTTTTGGGCT TTTTAAAAAA AAAATCCCCC CCTCCTCCCA TCGGGGTTTT
  541 GCCCCAAAAA TTAAAGGGGG GTTTTTTTTT CCAAAAGGGG GGAGGGAAAA ACGGGGGCCT
  601 TGGGTTTACT GGGGTTGCCA AAAAATTTGG GGGAAAAAGG GGCTTGGCCC GGACCGGGCC
  661 CGGGATTTAA AGACCCCCGT TTAAACAAAT TTGATTGGAT TGGAAACTGA CTCAGGGGTC
  721 ACTCGAACTG GAGTTGGTCC TGATCGGAGT CGAACAAAAC CCTAGCCCTT GTTGACCCGA
  781 ATCAACTGAG ATGGAACGAC CCAATTCTGG ACAACCTGAT ACAGAATGCC ACCACCCACC
  841 ATGATCCGCA TATCTGCACA CCACCATGCT TGCAGTTGAA ACGTGCAGGA TAAGTGCGCG
  901 TATCGACGCA ACTGAACGAC CTGGACTACA CTGACAGACT GATAATTCAG ACAGTTCGAC
961 ACTCGAGCAC TCGAG
//
Primer: SV40pro_F
Sequence file: RDB12204_A5Hpd.seq check
>12204_12204_A5Hp_SV40pro_F_H04_22.ab1
    1 GCCCTGTCCT TGGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG CGAAATGACC GACCAAGCGA
  961 CGCCCAACCC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAAGGTTGGG
 1021 CTTCGGAATC GTTTCCGGAC GCCGGGCTGG ATGATCTCAG CGCGGGGATC TCATGCTGGA
 1081 GTTCTCGCCC ACCCCACTGT TTATTGCAGC TTATATTGTA CAAATTAAAG CAATAGCATT
1141 CACGATTCAC GAATAGCATT TCACTGGCAA TCTCTTAATC TGTTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]