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HA-Amot130-delta CC/pcDNA3.1-pA83 (#RDB12205)

Expression clone of mouse Amot (p130), deletion of CC domain

Clone info. Expression clone of mouse Amot (p130), deletion of coiled-coil (CC) domain. HA-tag.
Comment See Fig. 4A of Hirate et al., Curr. Biol. 23: 1181-1194 (2013). The expression vector has a stretch of poly(A).
Vector backbone pcDNA3.1-pA83 (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name Mouse Amot cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12205 HA-Amot130-delta CC/pcDNA3.1-pA83 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The HA-Amot130-delta CC/pcDNA3.1-pA83 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12205).

Reference section:

Hirate, Y., Hirahara, S,. Inoue, K., Suzuki, A., Alarcon, V.B., Akimoto, K., Hirai, T., Hara, T., Adachi, M., Chida, K., Ohno, S., Marikawa, Y., Nakao, K., Shimono, A., Sasaki, H., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12205_A5Hpp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB12205_A5Hpa.seq check
>12205_12205_A5Hp_CMV-Forward_F11_17.ab1
    1 TTGTTTCACT CCGCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGTTAAGCTA GCTTGCCGCC ACCATGTACC
  181 CATACGACGT TCCAGATTAC GCTGGATCCA GAAGTTCTGA CGATCAGCCA AGTGGAGGAA
  241 CGACGGTACT GCAACGGCTG CTTCAAGAGC AGCTTCGCTA TGGCAATCCC AGTGAGAATC
  301 GCAGTCTGCT TGCCATACAC CAGCAAGCCA CAGGGAATAG CTCTCCATTT TCCACGGGCA
  361 GTGGGAACCA AGGCCCTCAG AATGATGTAT TGAGTTCCCA GGATCACCAC CAGCAGCAGC
  421 TTGTGGCACA CCCTGCTCGC CAAGAGCCCC AGGGTCAGGA AATCCAGTCA GAAAACGGCG
  481 TCATGGAGAA GCAGCTGTCC CCTCGAATGC AGAATAACGA AGAACTCCCA ACCTATGAAG
  541 AGGCCAAGGT CCAGTCCCAG TACTTCAGGG GCCAGCAGCA TGCTAGTGTT GGGGCTGCTT
  601 TCTATGTCAC AGGAGTCACC AACCAGAAGA TGAGAACCGA GGGACGCCCG TCAGTCCAGC
  661 GCCTCACGCC CGGGAAGATG CACCAAGATG AAGGCCTTCG AGATCTTAAG CAAGGGCATG
  721 TCCGATCCTT GAGTGAAAGA CTGATGCAGA TGTCATTGGC TACTAGTGGA GTTAAAGCCC
  781 ACCCACCTGT TACCAGTGCT CCTCTCTCCC CACCACAACC CAATGATCTC TACAAGAATG
  841 CCACCAGTTC CAGTGAATTC TACAAGGCCC AAGGGCCACC TCCAAGCCAA CATAGTCTGA
  901 AAGGGCATGG AACACCGAGG TCCCCCGCCA GAATACCCTT TCAAGGGCGT GCCATCCCAG
  961 TCTGTAGTGT GTAAGTCCCA AGAGCCAGGG CACTTCTATA GTGAGCATCG CCTGAACCAG
 1021 CCAGGGAGAA CAGAGGGGCA ACTGATGAGT ATCAGCACCC CCCCTGAGTA TGGGGCAGCC
 1081 AGGGCAACAC AGAACATCTC CTTCGCTGTC TTTTGTCAGC AGGAAACTCT CACTTCATAG
1141 CCCTTACTCA TCCTGAGGCT TGAAGGACCT TTCTCCCCAG
//
Primer: mAmot-M#1
Sequence file: RDB12205_A5Hpb.seq check
>12205_12205_A5Hp_mAmot-M#1_H08_23.ab1
    1 GGGGGAAGCT ACTCAGCTAT GCCTCGGGCT CAGCAGTCTG CTTCTTATCA GCCCATGCCA
   61 GCTGACCCTT TTGCTATCAG ACACTTTGCT CTGGATGCTG CTGCAACTGT GGCTGCACAG
  121 AGAGACACAA CAGTCATTAG CCACTCTCCT AACACCAGCT ATGACACAGC CTTAGAGGCC
  181 CGCATCCAGA AGGAAGAGGA AGAAATCCTG ATGGCCAACA AGCGTTGCTT GGACATGGAA
  241 GGAAGGATTA AGACCCTCCA TGCCCAGATT ATTGAAAAGG ATGCCATGAT CAAGGTGCTG
  301 CAGCAGCGCT CCAGAAAGGA GCCGAGTAAA ACAGAGCAAC TCTCATCCAT GCGGCCAGCA
  361 AAGTCTCTGA TGTCCATTTC CAATGCTGGA TCAGGCCTCC TTGCACACTC CTCTACCTTG
  421 ACTGGTGCGC CCATTATGGA GGAGAAGCGT GATGACAAGA GCTGGAAGGG GAGCCTAGGC
  481 GTTCTTCTGG GAGGAGATTA CCGTGTTGAG CCGGTCCCAT CCACACCGTC CCCTGTGCCC
  541 CCGTCTACTC CCCTTCTCTC AGCTCATTCG AAGACAGGCA GTCGCGATTG CAGCACCCAA
  601 ACTGAACGTG GACCGGAATC GACCAAAACC GCAGCTGTCA CTCCCATCTC GGCTCCAATG
  661 GCTGGTCCAG TTGCTGCTGC TGCCCCTGCT GCTGCCATCA ATGCAACTGC TGCCACCAAC
  721 ACTGCCACTG CTGCCACCAA CACCACTATC ATGGTAGCTG CTGCTCCAGT TGCTGTTGCT
  781 GCTCCAGCTG CTGCTGCCGC CACCCCATCT CCAGCAAACG CTGCTGCTCT TGCCGCTGCT
  841 GCCGCTCCAG CTACTTCTGT CTCTGCAGCT ACTTCTGTCT CTGCAGCTAA TTCTATCTCT
  901 CCAGCTGCTC CTGTCGCTCC AGCTGCTGTA GTGCCTCCAG CTGCTCCTGT CTCTCCAGCT
  961 GCTGCTGTTC AGATTCCAGC TGCTGCATCT CTTACTCCTG CTACTGTTTC TCCAACTGCT
 1021 GCCACTGCTA CTGCTGCTGT GCTGCAGCTA CTACTGCTGC TATCACTGCT GCTGCTGCTG
 1081 CTGCACTACT GCTATTCACT GCTCGAGCTA CTCGCTCGAT CATCTCCAGC CTTCGATTCG
1141 GGCTCAGGCA ACGGGCCTTC AAGCT
//
Primer: GNP_primer#10
Sequence file: RDB12205_A5Hpc.seq check
>12205_12205_A5Hp_GNP_primer#10_B05_05.ab1
    1 CTTATTTGTG CCATTCATTT AAAAGATAAC GTTTACAGGT TCTTTGGTGC CCATAGTCGA
   61 GCGGCCAGCT TTAGAGCACA TTCTGCTTAA CCTATCTTTT TGAGGTACTC CCTAAGTATT
  121 CCAAAATAGA TTGGAATTCG TTTCTATGGT ATTACTTATG TACTACCAGA GTCCAATAAA
  181 AAGTCCCTTT AGAAAAATCA TCCCCACCTC CCCACCCTAT CTTCACCCCA GATTAAACAA
  241 ATGAAAGGGA AAAATTATTA AAAAGAACTC TTGCTGATAA CGCCAGCTCT TGATTGTGCT
  301 GTTTAGATGA GATATTCCAC CATCTCTGCA TCAGGCTCTT GCCCCAGGAT CTGAATGGGT
  361 GTCTTTCTTT CCAGAGTGCT GGAGTGGAAA GCAGGGGCAT CTGGCTTATC TGGGTTGCAC
  421 ATCAGATTTG GTGTAGACAA GCGGCGTGGT CCGGAACTGG CACCTGGATT TGCAGGACCC
  481 TCAGTTTGAA CCAAAGCTGG AGTTGGAGTT GGAACTGGAC TAGGAGGTTC AGTCGGAGCT
  541 GGAGTTGATG CCTGAGTCGG AGTCGGAGCA GAAGCCTGAG CCGTTGCTGG AGCCGGAATC
  601 GAAGCTGGAG ATGGAACCGG AGCCGAAGTA GCTGGAGCAA CCTGAATAGC AGTAGTGGCA
  661 GCAGCAGCAG CAGCAGTGAT AGCAGCAGTA GTAGCTGCAG CAACAGCAGC AGTAGCAGTG
  721 GCAGCAGTTG GAGAAACAGT AGCAGGAGTA AGAGATGCAG CAGCTGGAAT CTGAACAGCA
  781 GCAGCTGGAG AGACAGGAGC AGCTGGAGGC ACTACAGCAG CTGGAGCGAC AGGAGCAGCT
  841 GGAGAGATAG AATTAGCTGC AGAGACAGAA GTAGCTGCAG AGACAGAAGT AGCTGGAGCG
  901 GCAGCAGCGG CAAGAGCAGC AGCGTTTGCT GGAGATGGGG TGGCGGCAGC AGCAGCTGGA
  961 GCAGCAACAG CAACTGGAGC AGCAGCTACC ATGATAGTGG TGTTGGTGGC AGCAGTGGCA
 1021 GTGTTGGTGG CAGCAGTTGC ATTGATGGCA GCAGCAGGGG CAGCAGCAGC AACTGGACCA
 1081 GCCATTGAGC CGAGATGGGG AGTGACAGCT GCGGATTTGG TCGATTCCGG TTCCACGTTT
 1141 CAGTGGGTGC CTGCAATCGC GACTGCCGGC TTCGATGACT GAAGAAGAGG GAATTAAACG
1201 GGGGTCAG
//
Primer: BGH_rev
Sequence file: RDB12205_A5Hpd.seq check
>12205_12205_A5Hp_BGH_rev_C05_08.ab1
    1 CTCTACTCAT GGTGATGGTG ATGATGACCG GTATGCATAT TCAGATCCTC TTCTGAGATG
   61 AGTTTTTGTT CGAAGGGCCC TCTAGACTCG AGGTACGCCA CAGCGTATGA TGCGTATTTT
  121 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  181 TTTTTTCCTT CCCCAAATTT TTTTTGGGCC CCTTTCATTT AAAAAAAAAA ACTTTTAGGG
  241 GTTTTTGGGG CCCCAAACCC AAGGGCCCCT TTTAAAAACC CTTTCGGTTA ACCCTTTTTT
  301 TTGGGGGCCC CCCCAAATTT TCCCAAAAAA AATGGGATTT TTTTTCTGGG GTTTTTTTTT
  361 GGCCCCCCCA AACCCCAAAA AAAACCCCTT TAAAAAAAAC CCCCCCCCCC CCCCCCCCCT
  421 TTTTCCCCCC CAAATTAACA AAGGGGGGGG AAAAATTTTT TAAAAAAACC TTTTGTTAAA
  481 ACCCCCCCCC CTGGTTGGGG TGGTTAAAAA AAAAATTCCC CCCCCCCCCG CACCGGGCTT
  541 TCCCCCCAAA ATTAAAGGGG GGTTTTTTTT TCCCAAAGGG GGGGGGGGAA AAAGGGGGGC
  601 CCCGGGTTTT TGGGGGTGCC CCCCAAAATT GGGGGAACCC CAGGGGGGGG GCCCGGAACG
  661 GGCCCGGTTT TTCCGGGCCT TCTTGTGGGC CCACCTGAAT TGAGTTTGGA CTGATAGAGC
  721 TTCCCGGAGC GGATTAGGGC GGGACCGAAC CGAACAACCG GACGTTGCGG ACCGATCACT
  781 GGATGACCGG ACAATTCTGG ACACTGAATC AATGCCCCCC CCCAGTACCC ATACTGACAC
  841 CCCATACTGC AGTGCATCTC AGATTAATGC CGCTGATCGA CACCTTGAAC CGACCTTGGA
  901 CTCATCGTTG ACAGGATCTG ACTATTCCGA CAAGTGCGCA TATCCAGTCA ACGGAGCCGT
961 G
//
Primer: SV40pro_F
Sequence file: RDB12205_A5Hpe.seq check
>12205_12205_A5Hp_SV40pro_F_D05_11.ab1
    1 CCCTGTCCTT GGCATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG
  121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG
  661 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  781 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  841 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  901 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGC GAAATGACCG ACCAAGCGAC
  961 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAG GTTGGGCTTC
 1021 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGATCTC ATGCTGAGTT
 1081 CTTCGCCCAC CCCCAACTGT TTATTGCAGC TTATATGGTT ACAAATTAGG CCATAGCATC
1141 ACAATTCACA ATAAGCATTT TTTTCCACCT GCCATTTCTC TATAGTTGTG TTGGATG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]