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HA-Amot130-delta PDZbd/pcDNA3.1-pA83 (#RDB12207)

Expression clone of mouse Amot (p130), deletion of PDZbd

Clone info. Expression clone of mouse Amot (p130), deletion of PDZ domain-binding motif (PDZbd). HA-tag.
Comment See Fig. 4A of Hirate et al., Curr. Biol. 23: 1181-1194 (2013). The expression vector has a stretch of poly(A).
Vector backbone pcDNA3.1-pA83 (Plasmid)
Size of vector backbone 5.6 kb
Selectable markers Amp^r
Gene/insert name Mouse Amot cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12207 HA-Amot130-delta PDZbd/pcDNA3.1-pA83 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The HA-Amot130-delta PDZbd/pcDNA3.1-pA83 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12207).

Reference section:

Hirate, Y., Hirahara, S,. Inoue, K., Suzuki, A., Alarcon, V.B., Akimoto, K., Hirai, T., Hara, T., Adachi, M., Chida, K., Ohno, S., Marikawa, Y., Nakao, K., Shimono, A., Sasaki, H., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12207_A5Hpp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB12207_A5Hpa.seq check
>12207_12207_A5Hp_CMV-Forward_E09_15.ab1
    1 TTTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTGGCTAGTT AAGCTTGCCG CCACCATGTA CCCATACGAC
  181 GTTCCAGATT ACGCTGGATC CAGAAGTTCT GACGATCAGC CAAGTGGAGG AACGACGGTA
  241 CTGCAACGGC TGCTTCAAGA GCAGCTTCGC TATGGCAATC CCAGTGAGAA TCGCAGTCTG
  301 CTTGCCATAC ACCAGCAAGC CACAGGGAAT AGCTCTCCAT TTTCCACGGG CAGTGGGAAC
  361 CAAGGCCCTC AGAATGATGT ATTGAGTTCC CAGGATCACC ACCAGCAGCA GCTTGTGGCA
  421 CACCCTGCTC GCCAAGAGCC CCAGGGTCAG GAAATCCAGT CAGAAAACGG CGTCATGGAG
  481 AAGCAGCTGT CCCCTCGAAT GCAGAATAAC GAAGAACTCC CAACCTATGA AGAGGCCAAG
  541 GTCCAGTCCC AGTACTTCAG GGGCCAGCAG CATGCTAGTG TTGGGGCTGC TTTCTATGTC
  601 ACAGGAGTCA CCAACCAGAA GATGAGAACC GAGGGACGCC CGTCAGTCCA GCGCCTCACG
  661 CCCGGGAAGA TGCACCAAGA TGAAGGCCTT CGAGATCTTA AGCAAGGGCA TGTCCGATCC
  721 TTGAGTGAAG ACTGATGCAG ATGTCATTGG CTACTAGTGG AGTTAAAGCC CACCCACCTG
  781 TTACCAGTGC TCCTCTCTCC CCACCACAAC CCAATGATCT CTACAAGAAT GCCACCAGTT
  841 CCAGTGAATT CTACAAGGCC CAAGGGCCAC CTCCAAGCCA ACATTAAGTC TGAAGGGCAT
  901 GAAACACCGA GGTCCCCCGC CAGAATACCC TTTTCAAGGG CGTGCCATCC CAGTCTGTAA
  961 GTGTGTAAGT CCCAAGAGCC AGGGCACTTC TATAGTGAGC ATCGCCTGAA CCAGCCAGGG
 1021 AGAACAGAGG GGCAACTGAA TGAGTATCAG CACCCCCCCT GAAGTTATGG GGGCAGTCAA
 1081 GGCAACACAG GAACATCCTC CTCGCCTGTT CTTTTGTCAG TAGACTCTCC AGCTCATTAG
1141 GCCTACATCT TCCCTGGAAA CAGAGCTTTG TG
//
Primer: GNP_primer#10
Sequence file: RDB12207_A5Hpb.seq check
>12207_12207_A5Hp_GNP-primer#10_F09_18.ab1
    1 TTATTTGTGC CATTCATTTA AAAGATAACG TTTACAGGTT CTTTGGTGCC CATAGTCGAG
   61 CGGCCGCTTA CACCATCTCT GCATCAGGCT CTTGCCCCAG GATCTGAATG GGTGTCTTTC
  121 TTTCCAGAGT GCTGGAGTGG AAAGCAGGGG CATCTGGCTT ATCTGGGTTG CACATCAGAT
  181 TTGGTGTAGA CAAGCGGCGT GGTCCGGAAC TGGCACCTGG ATTTGCAGGA CCCTCAGTTT
  241 GAACCAAAGC TGGAGTTGGA GTTGGAACTG GACTAGGAGG TTCAGTCGGA GCTGGAGTTG
  301 ATGCCTGAGT CGGAGTCGGA GCAGAAGCCT GAGCCGTTGC TGGAGCCGGA ATCGAAGCTG
  361 GAGATGGAAC CGGAGCCGAA GTAGCTGGAG CAACCTGAAT AGCAGTAGTG GCAGCAGCAG
  421 CAGCAGCAGT GATAGCAGCA GTAGTAGCTG CAGCAACAGC AGCAGTAGCA GTGGCAGCAG
  481 TTGGAGAAAC AGTAGCAGGA GTAAGAGATG CAGCAGCTGG AATCTGAACA GCAGCAGCTG
  541 GAGAGACAGG AGCAGCTGGA GGCACTACAG CAGCTGGAGC GACAGGAGCA GCTGGAGAGA
  601 TAGAATTAGC TGCAGAGACA GAAGTAGCTG CAGAGACAGA AGTAGCTGGA GCGGCAGCAG
  661 CGGCAAGAGC AGCAGCGTTT GCTGGAGATG GGGTGGCGGC AGCAGCAGCT GGAGCAGCAA
  721 CAGCAACTGG AGCAGCAGCT ACCATGATAG TGGTGTTGGT GGCAGCAGTG GCAGTGTTGG
  781 TGGCAGCAGT TGCATTGATG GCAGCAGCAG GGGCAGCAGC AGCAACTGGA CCAGCCATTG
  841 GAGCCGAGAT GGGAGTGACA GCTGCGGTTT TGGTCGATTC CGGTCCACGT TCAGTTTGGG
  901 TGCTGCAATC GCGACTGCCT GTCTTCGAAT GAGCTGAGAG AAAGGGGAGT AGACGGGGGC
  961 ACAGGGGACG GGGTGGTGGG ATGGGGACCG GCTCAACACG GGTAATCTCC TCCTCAGAGA
 1021 ACGCCTAGCT CCCCTTTCCA GCTCTTGTCA TCACGCCTTC TCTCATAATG GCGCACAGTC
 1081 AAAGGTAGAG GAGTGTGCAA GAAGGCCTGA ATCAGCATGG AAATTGAACA TCAGAGACCT
1141 TTGCTGGGCG CGCCCATTT
//
Primer: BGH_rev
Sequence file: RDB12207_A5Hpc.seq check
>12207_12207_A5Hp_BGH_rev_B11_05.ab1
    1 CTCTCTCATG GTGATGGTGA TGATGACCGG TATGCATATT CAGATCCTCT TCTGAGATGA
   61 GTTTTTGTTC GAAGGGCCCT CTAGACTCGA GGTACGCCAC AGCGTATGAT GCGTATTTTT
  121 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  181 TTTTTCCTTT CCCAATTTTT TTTTTGGCCC CTTTCTTTTA AAAAAAAAAA TTTTTAAGGT
  241 TTTTTGGGGC CCCAAAAAAA AGGGGCCCTT TACCCCCTTT TTCCTTAGGG TTTTTCCCCC
  301 AAAATTAAAG GGGGGTTTTT TTTTCCCAAA GGGGGGGGGG GAAAAAAGGG GCCTTTGGTT
  361 TTTTTGGGTT TCCCCCAAAA ATTTGGGGAA AAAAACGGGG GGGGCCCAAA ATGGGCCCCT
  421 GTTTTTCCGG ACCCCCTTTT TTAACCCAAA CTGGGATTGG AATTTGAACC GGGCCAAAAG
  481 GTTCCCCCCA AACTGGGATT TAATCCCTAA TCCAAATCCA AAAAAAAACC CTGACCCTTT
  541 TGGGGGCCCG AAATCAAACT GGGAAAGGAA CCCGAACCCA AATTTTTGGG AAAACCTATT
  601 TTCCATTTGG GGCCCCACAC ACCCCCTTGT AAACACATTA TAACTCGGCA CACACACTTT
  661 CCGTTGCCGC AGTGGAAACC TTCGAGGATT AAAGGCCGCT GGATTGAACA CCTCTGGAAA
  721 CAGACACTGG AGCCTCACTT GACCAGACCT GGAAAGATGT CGAACAATCT CGCAACAATC
  781 TGACGCTCGC GAGCACGGTC CGATTTGTTG CCACACTGAC ACACTTGACT TATCGTGACA
841 GCATTGACAA GGTTGGCACA G
//
Primer: SV40pro_F
Sequence file: RDB12207_A5Hpd.seq check
>12207_12207_A5Hp_SV40pro_F_H09_24.ab1
    1 CCCTGTCCTG TACATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG
  121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG
  661 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  781 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  841 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  901 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG CGAAATGACC GACCAAGCGA
  961 CGCCCAACCT TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTAATG AAAGGGTTGG
 1021 GCTTTCGGAA TCGTTTTCCG GACGCCCGGG CCTGATGATC CTCCAGCGCG GGGATCTTCA
 1081 TGCCTGAAGT CTCGGCCCAC CCCACCTTGG TTAATGCAGC CTATATGCTT ACCACTAAGG
1141 CCATAGCATT CCCACACTTT TCCACAAATA AGAGCGC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]