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HA-Amot130-S176A/pcDNA3.1-pA83 (#RDB12208)

Expression clone of mouse Amot (p130), S176A mutant

Clone info. Expression clone of mouse Amot (p130), S176A mutant. HA-tag.
Comment See Fig. 5G of Hirate et al., Curr. Biol. 23: 1181-1194 (2013). The expression vector has a stretch of poly(A).
Vector backbone pcDNA3.1-pA83 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Amot cDNA
Depositor|Developer Sasaki, Hiroshi |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12208 HA-Amot130-S176A/pcDNA3.1-pA83 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The HA-Amot130-S176A/pcDNA3.1-pA83 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12208).

Reference section:

Hirate, Y., Hirahara, S,. Inoue, K., Suzuki, A., Alarcon, V.B., Akimoto, K., Hirai, T., Hara, T., Adachi, M., Chida, K., Ohno, S., Marikawa, Y., Nakao, K., Shimono, A., Sasaki, H., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12208_A5Hpp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB12208_A5Hpa.seq check
>12208_12208_A5Hp_CMV-Forward_C11_08.ab1
    1 TGTAACTCCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGGCTAGT TAAGCTAGCT TGCCGCCACC ATGTACCCAT
  181 ACGACGTTCC AGATTACGCT GGATCCAGAA GTTCTGACGA TCAGCCAAGT GGAGGAACGA
  241 CGGTACTGCA ACGGCTGCTT CAAGAGCAGC TTCGCTATGG CAATCCCAGT GAGAATCGCA
  301 GTCTGCTTGC CATACACCAG CAAGCCACAG GGAATAGCTC TCCATTTTCC ACGGGCAGTG
  361 GGAACCAAGG CCCTCAGAAT GATGTATTGA GTTCCCAGGA TCACCACCAG CAGCAGCTTG
  421 TGGCACACCC TGCTCGCCAA GAGCCCCAGG GTCAGGAAAT CCAGTCAGAA AACGGCGTCA
  481 TGGAGAAGCA GCTGTCCCCT CGAATGCAGA ATAACGAAGA ACTCCCAACC TATGAAGAGG
  541 CCAAGGTCCA GTCCCAGTAC TTCAGGGGCC AGCAGCATGC TAGTGTTGGG GCTGCTTTCT
  601 ATGTCACAGG AGTCACCAAC CAGAAGATGA GAACCGAGGG ACGCCCGTCA GTCCAGCGCC
  661 TCACGCCCGG GAAGATGCAC CAAGATGAAG GCCTTCGAGA TCTTAAGCAA GGGCATGTCC
  721 GATCCTTGGC CGAAAGACTG ATGCAGATGT CATTGGCTAC TAGTGGAGTT AAAGCCCACC
  781 CACCTGTTAC CAGTGCTCCT CTCTCCCCAC CACAACCCAA TGATCTCTAC AAGAATGCCA
  841 CCAGTTCCAG TGAATTCTAC AAGGCCCAAG GGCCACCTCC AAGCCAACAT AGTCTGAAGG
  901 GCATGGAACA CCGAGGTCCC CCGCCAGAAT ACCCTTTCAA GGGCGTGCCA TCCCAGTCTG
  961 TAGTGTGTAA GTCCCAAGAG CCAGGGCACT TCTATAGTGA GCATCGCCTG AACCAGCCAG
 1021 GGAGACAGAG GGGCAACTGA TGAGGTATCA GCACCCCCCT GAGTATGGGG CAGCCAGGGC
 1081 AACACAGACA TCTCTCGCTG TTCTTTTGTC AGCAGACTCT CACTTCATAG TCCTTACTCC
1141 TCCCTGACAA GCTTGGGGGC TTCCCAGGCC ATTGG
//
Primer: GNP_primer#10
Sequence file: RDB12208_A5Hpb.seq check
>12208_12208_A5Hp_GNP_primer#10_A09_03.ab1
    1 GTATTTTTGT GCAAATTCAT TTAAAAGATA ACGTTTACAG GTTCTTTGGT GCCCATAGTC
   61 GAGCGGCCAG CTTTAGAGCA CATTCTGCTT AACCTATCTT TTTGAGGTAC TCCCTAAGTA
  121 TTCCAAAATA GATTGGAATT CGTTTCTATG GTATTACTTA TGTACTACCA GAGTCCAATA
  181 AAAAGTCCCT TTAGAAAAAT CATCCCCACC TCCCCACCCT ATCTTCACCC CAGATTAAAC
  241 AAATGAAAGG GAAAAATTAT TAAAAAGAAC TCTTGCTGAT AACGCCAGCT CTTGATTGTG
  301 CTGTTTAGAT GAGATATTCC ACCATCTCTG CATCAGGCTC TTGCCCCAGG ATCTGAATGG
  361 GTGTCTTTCT TTCCAGAGTG CTGGAGTGGA AAGCAGGGGC ATCTGGCTTA TCTGGGTTGC
  421 ACATCAGATT TGGTGTAGAC AAGCGGCGTG GTCCGGAACT GGCACCTGGA TTTGCAGGAC
  481 CCTCAGTTTG AACCAAAGCT GGAGTTGGAG TTGGAACTGG ACTAGGAGGT TCAGTCGGAG
  541 CTGGAGTTGA TGCCTGAGTC GGAGTCGGAG CAGAAGCCTG AGCCGTTGCT GGAGCCGGAA
  601 TCGAAGCTGG AGATGGAACC GGAGCCGAAG TAGCTGGAGC AACCTGAATA GCAGTAGTGG
  661 CAGCAGCAGC AGCAGCAGTG ATAGCAGCAG TAGTAGCTGC AGCAACAGCA GCAGTAGCAG
  721 TGGCAGCAGT TGGAGAAACA GTAGCAGGAG TAAGAGATGC AGCAGCTGGA ATCTGAACAG
  781 CAGCAGCTGG AGAGACAGGA GCAGCTGGAG GCACTACAGC AGCTGGAGCG ACAGGAGCAG
  841 CTGGAGAGAT AGAATTAGCT GCAGAGACAG AAGTAGCTGC AGAGACAGAA GTAGCTGGAG
  901 CGGCAGCAGC GGCAAGAGCA GCAGCGTTTG CTGGAGATGG GGTGGCGGCA GCAGCAGCTG
  961 GAGCAGCAAC AGCAACTGGA GCAGCAGCTA CCATGATAGT GGTGTTGGTG GCAGCAGTGG
 1021 CAGTGTTGGT TGGCAGCAGT TGCATTGATG GCAGCAGCAG GCAGCAGCAG CAACTGGACC
 1081 AGTCCATTGC AGCCGAGATG GGGAGTGACA GCTTGCGCTT TGTCGGATTC AGTTCCACGT
1141 TCAGCTTGGG GTTGCCTGGC CAAATCCGCC GAACCCTGTG TCC
//
Primer: BGH_rev
Sequence file: RDB12208_A5Hpc.seq check
>12208_12208_A5Hp_BGH_rev_B09_06.ab1
    1 CCATTACCTT TATGGTGATG GTGATGATGA CCGGTATGCA TATTCAGATC CTCTTCTGAG
   61 ATGAGTTTTT GTTCGAAGGG CCCTCTAGAC TCGAGGTACG CCACAGCGTA TGATGCGTAT
  121 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  181 TTTTTTTTTT CCTTCCCCCA CTTTTTTTTT GGCCCCTTTC TTTTAAAAAA AAAACCTTTT
  241 CGGGGTTTTT GGGGCCCCAA ACCCGGGGGC CCCTTTTTAA ACCCCTTCCG CTTAACCCTT
  301 TTTTTTGGGG GACCCCCCAA AAATTCCAAA AAAAAAGGGA AATCCTTCCC TGGGGTTTTC
  361 TTTTGGCCCC CCCAAACCCC AAAAAAAACC CCTTTAAAAA AAAACCCCCC CCCCCCCCCC
  421 CCCTTTTTCC CCCCCAATTT AAAAAAAGGA GGGGAAAAAT TTTTTAAAAA AACTTTTTGG
  481 GAAAACCCCC CCCTTTTGTT GGGGGTTTTA AAAAAAAAAT TCCCCCCCCC CCGGCCCAGG
  541 GCTTTTCCCC CAAAATTAAA GGGGGGTTTT TTTTTCCCAA AGGGGGGGGG GGAAAACCGG
  601 GGGCTTTGGG TTTTTCGGGG GGGCACCCAA AATTGGGGGA AAACAACGGG GGGGGCCCGG
  661 AATGGGCCCG GTTTTGGGGG CCCTCATTTG CCCCAACTGG GTTGGGGTTT GACTGCCCTA
  721 GATGTCATCC GAACTGAAAT AACCCTGATC CGGACCCGGA CAAATCTGCG CTGTGTGACC
  781 CAATCAAACT TGAGAGGACC CGGACCAATT CTGACACCTG ATACCATAGG CAACCACCCA
  841 CTGATACACT ATAATCGTCG ACACAACATA TCATTGCAAC ATGGGAAACT TACAGATTAA
  901 GTGCAGTCTG GATCCGCACT GACGTTTGAA CCGAACCTGA GTACTACACA CTGGGAACGA
961 TGTGCTATAG ACATTTAAAT AGGCGTAACA GTATTCGGGA CATACGAA
//
Primer: SV40pro_F
Sequence file: RDB12208_A5Hpd.seq check
>12208_12208_A5Hp_SV40pro_F_C09_09.ab1
    1 CCTTTGTTCT CCGTGCCAAT CAGGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT
   61 TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA
  121 GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG
  181 GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG
  241 TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC
  301 CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT
  361 TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA
  421 GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG
  481 GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA
  541 GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT
  601 GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG
  661 CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC
  721 ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC
  781 CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG
  841 GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC
  901 TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GGTTCGCGAA ATGACCGACC
  961 AAGCGACGCC CAACCTGCCC ATCACGAGAT TTCGATTCCA CCGCCGCCCT TCCTATGAAA
 1021 GGTTGGGCTT TCGGAATCCG TTTTTCCGGG ACCGCCCGGC CTGGGATGAT CCTCCAGCGG
 1081 CGGGGGAATC TCCATGCTTG GAGTCTTCGC CCACCCCAAC CTTGGTTAAT GCCAGCTATA
1141 TTGGTTTAAC AAGTCAAGCA AATAAGCCAT CCAACCAAAC TTTCTCCACC GAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]