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HA-Amot130-S176E/pcDNA3.1-pA83 (#RDB12209)

Expression clone of mouse Amot (p130), S176E mutant

Clone info. Expression clone of mouse Amot (p130), S176E mutant. HA-tag.
Comment See Fig. 5G of Hirate et al., Curr. Biol. 23: 1181-1194 (2013). The expression vector has a stretch of poly(A).
Vector backbone pcDNA3.1-pA83 (Plasmid)
Selectable markers Amp^r
Gene/insert name Mouse Amot cDNA
Depositor|Developer Sasaki, Hiroshi |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12209 HA-Amot130-S176E/pcDNA3.1-pA83 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The HA-Amot130-S176E/pcDNA3.1-pA83 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12209).

Reference section:

Hirate, Y., Hirahara, S,. Inoue, K., Suzuki, A., Alarcon, V.B., Akimoto, K., Hirai, T., Hara, T., Adachi, M., Chida, K., Ohno, S., Marikawa, Y., Nakao, K., Shimono, A., Sasaki, H., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12209_A5Hpp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB12209_A5Hpa.seq check
>12209_12209_A5Hp_CMV-Forward_G11_20.ab1
    1 TTTTTCAACT CCGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGTTAAGCTA GCTTGCCGCC ACCATGTACC
  181 CATACGACGT TCCAGATTAC GCTGGATCCA GAAGTTCTGA CGATCAGCCA AGTGGAGGAA
  241 CGACGGTACT GCAACGGCTG CTTCAAGAGC AGCTTCGCTA TGGCAATCCC AGTGAGAATC
  301 GCAGTCTGCT TGCCATACAC CAGCAAGCCA CAGGGAATAG CTCTCCATTT TCCACGGGCA
  361 GTGGGAACCA AGGCCCTCAG AATGATGTAT TGAGTTCCCA GGATCACCAC CAGCAGCAGC
  421 TTGTGGCACA CCCTGCTCGC CAAGAGCCCC AGGGTCAGGA AATCCAGTCA GAAAACGGCG
  481 TCATGGAGAA GCAGCTGTCC CCTCGAATGC AGAATAACGA AGAACTCCCA ACCTATGAAG
  541 AGGCCAAGGT CCAGTCCCAG TACTTCAGGG GCCAGCAGCA TGCTAGTGTT GGGGCTGCTT
  601 TCTATGTCAC AGGAGTCACC AACCAGAAGA TGAGAACCGA GGGACGCCCG TCAGTCCAGC
  661 GCCTCACGCC CGGGAAGATG CACCAAGATG AAGGCCTTCG AGATCTTAAG CAAGGGCATG
  721 TCCGATCCTT GGAAGAAAGA CTGATGCAGA TGTCATTGGC TACTAGTGGA GTTAAAGCCC
  781 ACCCACCTGT TACCAGTGCT CCTCTCTCCC CACCACAACC CAATGATCTC TACAAGAATG
  841 CCACCAGTTC CAGTGAATTC TACAAGGCCC AAGGGCCACC TCCAAGCCAA CATAGTCTGA
  901 AGGGCATGGA ACACCGAGGT CCCCCGCCAG AATACCCTTT CAAGGGCGTG CCATCCCAGT
  961 CTGTAGTGTG TAAGTCCCAA GAGCCAGGGC ACTTCTATAG TGAGCATCGC CTGAACCCAG
 1021 CCCAGGGAGA CAGAGGGCAA CTGATGAGGT ATCAGCAACC CCCCCTGAGT ATGGGGGCAG
1081 CCAGGCAACA CAGACATCCT CCTCGCTGAT CATGATCAGC CAAGGACT
//
Primer: GNP_primer#10
Sequence file: RDB12209_A5Hpb.seq check
>12209_12209_A5Hp_GNP_primer#10_H11_23.ab1
    1 TTATTTTGTG CAATTCATTT AAAAGATAAC GTTTACAGGT TCTTTGGTGC CCATAGTCGA
   61 GCGGCCAGCT TTAGAGCACA TTCTGCTTAA CCTATCTTTT TGAGGTACTC CCTAAGTATT
  121 CCAAAATAGA TTGGAATTCG TTTCTATGGT ATTACTTATG TACTACCAGA GTCCAATAAA
  181 AAGTCCCTTT AGAAAAATCA TCCCCACCTC CCCACCCTAT CTTCACCCCA GATTAAACAA
  241 ATGAAAGGGA AAAATTATTA AAAAGAACTC TTGCTGATAA CGCCAGCTCT TGATTGTGCT
  301 GTTTAGATGA GATATTCCAC CATCTCTGCA TCAGGCTCTT GCCCCAGGAT CTGAATGGGT
  361 GTCTTTCTTT CCAGAGTGCT GGAGTGGAAA GCAGGGGCAT CTGGCTTATC TGGGTTGCAC
  421 ATCAGATTTG GTGTAGACAA GCGGCGTGGT CCGGAACTGG CACCTGGATT TGCAGGACCC
  481 TCAGTTTGAA CCAAAGCTGG AGTTGGAGTT GGAACTGGAC TAGGAGGTTC AGTCGGAGCT
  541 GGAGTTGATG CCTGAGTCGG AGTCGGAGCA GAAGCCTGAG CCGTTGCTGG AGCCGGAATC
  601 GAAGCTGGAG ATGGAACCGG AGCCGAAGTA GCTGGAGCAA CCTGAATAGC AGTAGTGGCA
  661 GCAGCAGCAG CAGCAGTGAT AGCAGCAGTA GTAGCTGCAG CAACAGCAGC AGTAGCAGTG
  721 GCAGCAGTTG GAGAAACAGT AGCAGGAGTA AGAGATGCAG CAGCTGGAAT CTGAACAGCA
  781 GCAGCTGGAG AGACAGGAGC AGCTGGAGGC ACTACAGCAG CTGGAGCGAC AGGAGCAGCT
  841 GGAGAGATAG AATTAGCTGC AGAGACAGAA GTAGCTGCAG AGACAGAAGT AGCTGGAGCG
  901 GCAGCAGCGG CAAGAGCAGC AGCGTTTGCT GGAGATGGGG TGGCGGCAGC AGCAGCTGGA
  961 GCAGCAACAG CAACTGGAGC AGCAGCTACC ATGATAGTGG TGTTGGTGGC AGCAGTGGCA
 1021 GTGTTGGTGG CAGCAGTTGC ATTGATGGCA GCAGCAGGGC AGCAGCAGCA ACTGGACCAG
 1081 CATTGAGCCG AGATGGGAAG TGACAGCTGC GCTTTGTCGA TTCGGTCCAC GTCAGTTGGA
1141 TGCTGCATCG CGACTGCCTG TTCTTTCGAT GAGCTGGAAA GAAATGGGGA AGTTAGAC
//
Primer: BGH_rev
Sequence file: RDB12209_A5Hpc.seq check
>12209_12209_A5Hp_BGH_rev_A12_03.ab1
    1 CATACTCAAT GGTGATGGTG ATGATGACCG GTATGCATAT TCAGATCCTC TTCTGAGATG
   61 AGTTTTTGTT CGAAGGGCCC TCTAGACTCG AGGTACGCCA CAGCGTATGA TGCGTATTTT
  121 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  181 TTTTTTTCCT TCCCCAAATT TTTTTTGGGG CCCTTTCATT TAAAAAAAAA AACTTTTACG
  241 GGTTTTTGGG GCCCCAAACC CAAGGGCCCC TTTTTAAACC CCTTTCCGTT AACCCTTTTT
  301 TTTGGGGGAC CCCCCAAAAT TTCCCAAAAA AAAGGGAAAT TTTTTCCTGG GGGTTTCCTT
  361 TGGCCCCCCC AAACCCCAAA AAAAACCCCC TTTAAAAAAA TCCCCCCCCC CCCCCCCCCC
  421 TTTTTCCCCC CCAAATTAAA AAAGGGGGGG GGAAAATTTT TTAAAAAAAC CCTTGGGGAA
  481 AACCCCCCCC CTTTGTTGGG GCGTTTAAAA AAAAAAATCC CCCCCCCCCG GCATCGGGTT
  541 TTTCCCCCAA AATTAAAAGG GGGTTTTTTT TTTCCAAAGG GGGGGGGGGA AAACCGGGGG
  601 CCCCGGGTTA ACCGGGGGGC CCAAAAATTT GGGAAAAAAC GGGTTGTCCG AATGGCCCGG
  661 GTTTTAGGAC CCCCCTTTGA CCAAGGGGAT TGGATTTGAA CGGACAAGAG GTTCACCGAC
  721 TGGATTTAGC CTGAATCGAA TGGACAAAAC CCGACCCTTG CGGACCGAAT CAAACTGGAG
  781 ATGGACCGGA CCAAATTCCG GACCACCTGA TACCTAGTGC GACCACCCAC ACATGAAACC
  841 ACATTTTCTG CAACACACAC AAAACAGTGC ACATTGGAAC GTCGGATTAA ATGCCCCTGA
  901 TCGGACGCTC TGGAAACCGA TCCTGGTCTA CACTGGACCA AGACGTGCAG AATATCGCGG
961 ACAGATTCGC GGAGCAGATT CGAGTCACCG CAGC
//
Primer: SV40pro_F
Sequence file: RDB12209_A5Hpd.seq check
>12209_12209_A5Hp_SV40pro_F_D09_12.ab1
    1 CCCTGTCCTT GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
   61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
  121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
  661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
  781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
  901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGC GAAATGACCG ACCAAGCGAC
  961 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC CTTCTATGAA AGGTTGGGCT
 1021 TCGGAATCGT TTTCCCGGGA CGCCCGGCTG GGATGATCCT CCAGCGCGGG GGATCTCATG
 1081 CTGGGAGGTT CTTCGCCCAC CCCAAACTTG TTTATGCCAG CCTATATTGC TAACAAATAA
1141 AGCATAGCAT CACAAATTTT CCACCAAATT AAAA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]