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HA-Amot-delta(45-100)/pcDNA3.1-pA83 (#RDB12211)

Expression clone of mouse Amot (p130), lacking 45-100aa

Clone info. Expression clone of mouse Amot (p130), lacking amino acid residue 45-100. HA-tag.
Comment See Fig. 6D of Hirate et al., Curr. Biol. 23: 1181-1194 (2013). The expression vector has a stretch of poly(A).
Vector backbone pcDNA3.1-pA83 (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name Mouse Amot cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12211 HA-Amot-delta(45-100)/pcDNA3.1-pA83 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The HA-Amot-delta(45-100)/pcDNA3.1-pA83 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12211).

Reference section:

Hirate, Y., Hirahara, S,. Inoue, K., Suzuki, A., Alarcon, V.B., Akimoto, K., Hirai, T., Hara, T., Adachi, M., Chida, K., Ohno, S., Marikawa, Y., Nakao, K., Shimono, A., Sasaki, H., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12211_A5Hpp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB12211_A5Hpa.seq check
>12211_12211_A5Hp_CMV-Forward_F08_17.ab1
    1 TTTACTCCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
  121 CACTATAGGG AGACCCAAGC TGGCTAGTTA AGCTAGCTTG CCGCCACCAT GTACCCATAC
  181 GACGTTCCAG ATTACGCTGG ATCCAGAAGT TCTGACGATC AGCCAAGTGG AGGAACGACG
  241 GTACTGCAAC GGCTGCTTCA AGAGCAGCTT CGCTATGGCA ATCCCAGTGA GAATCGCAGT
  301 CTGCTTGCCA TACACCAGCA AGCCACAGGG AATGGATCCA TGCAGAATAA CGAAGAACTC
  361 CCAACCTATG AAGAGGCCAA GGTCCAGTCC CAGTACTTCA GGGGCCAGCA GCATGCTAGT
  421 GTTGGGGCTG CTTTCTATGT CACAGGAGTC ACCAACCAGA AGATGAGAAC CGAGGGACGC
  481 CCGTCAGTCC AGCGCCTCAC GCCCGGGAAG ATGCACCAAG ATGAAGGCCT TCGAGATCTT
  541 AAGCAAGGGC ATGTCCGATC CTTGAGTGAA AGACTGATGC AGATGTCATT GGCTACTAGT
  601 GGAGTTAAAG CCCACCCACC TGTTACCAGT GCTCCTCTCT CCCCACCACA ACCCAATGAT
  661 CTCTACAAGA ATGCCACCAG TTCCAGTGAA TTCTACAAGG CCCAAGGGCC ACCTCCAAGC
  721 CAACATAGTC TGAAGGGCAT GGAACACCGA GGTCCCCCGC CAGAATACCC TTTCAAGGGC
  781 GTGCCATCCC AGTCTGTAGT GTGTAAGTCC CAAGAGCCAG GGCACTTCTA TAGTGAGCAT
  841 CGCCTGAACC AGCCAGGGAG AACAGAGGGG CAACTGATGA GGTATCAGCA CCCCCCCTGA
  901 GTATGGGGCA GCCAGGGCAA CACAGGACAT CTCCTCGCTG TCTTTGTCAG CCAGGAACTC
  961 TCAACCTCAT AGCCCTACTT CTTCCCTGAC AGCTGGGGCT TCCAGCTTGC CCTTTGCTGC
 1021 AGTCTCCGCC GTCCACTAGA TTGCCTCCTG GTCACACCTG GTCCCGAACC AAGTGACACT
 1081 CTGCTCACTG TCAGGGCATC AGCAGCATCA TCTTCCGAGT CAGAGTCATC AGGGGGGACC
1141 AACTACCGGT TCCACGGCCC CC
//
Primer: GNP_primer#10
Sequence file: RDB12211_A5Hpb.seq check
>12211_12211_A5Hp_GNP-primer#10_F07_16.ab1
    1 TTATTGTGCC ATTCATTTAA AAGATAACGT TTACAGGTTC TTTGGTGCCC ATAGTCGAGC
   61 GGCCAGCTTT AGAGCACATT CTGCTTAACC TATCTTTTTG AGGTACTCCC TAAGTATTCC
  121 AAAATAGATT GGAATTCGTT TCTATGGTAT TACTTATGTA CTACCAGAGT CCAATAAAAA
  181 GTCCCTTTAG AAAAATCATC CCCACCTCCC CACCCTATCT TCACCCCAGA TTAAACAAAT
  241 GAAAGGGAAA AATTATTAAA AAGAACTCTT GCTGATAACG CCAGCTCTTG ATTGTGCTGT
  301 TTAGATGAGA TATTCCACCA TCTCTGCATC AGGCTCTTGC CCCAGGATCT GAATGGGTGT
  361 CTTTCTTTCC AGAGTGCTGG AGTGGAAAGC AGGGGCATCT GGCTTATCTG GGTTGCACAT
  421 CAGATTTGGT GTAGACAAGC GGCGTGGTCC GGAACTGGCA CCTGGATTTG CAGGACCCTC
  481 AGTTTGAACC AAAGCTGGAG TTGGAGTTGG AACTGGACTA GGAGGTTCAG TCGGAGCTGG
  541 AGTTGATGCC TGAGTCGGAG TCGGAGCAGA AGCCTGAGCC GTTGCTGGAG CCGGAATCGA
  601 AGCTGGAGAT GGAACCGGAG CCGAAGTAGC TGGAGCAACC TGAATAGCAG TAGTGGCAGC
  661 AGCAGCAGCA GCAGTGATAG CAGCAGTAGT AGCTGCAGCA ACAGCAGCAG TAGCAGTGGC
  721 AGCAGTTGGA GAAACAGTAG CAGGAGTAAG AGATGCAGCA GCTGGAATCT GAACAGCAGC
  781 AGCTGGAGAG ACAGGAGCAG CTGGAGGCAC TACAGCAGCT GGAGCGACAG GAGCAGCTGG
  841 AGAGATAGAA TTAGCTGCAG AGACAGAAGT AGCTGCAGAG ACAGAAGTAG CTGGAGCGGC
  901 AGCAGCGGCA AGAGCAGCAG CGTTTGCTGG AGATGGGGTG GCGGCAGCAG CAGCTGGAGC
  961 AGCACAGCAC TGGAGCAGCA GCTACCATGA TAGTGGTGTG TGCAGCAGTG CAGTGTGTGC
 1021 AGCAGTGCAT TGATGGCAGC AGCAGGGCAG CAGCAGCACT GGACAAGCAT GAGCGAGATG
 1081 GGAGTGACAG CCTGCGATTG GTCGAATTCG GTTCCACGGT CAGTTGGGGT GGCCTGCAAT
1141 TCCGCCGACT ATGACG
//
Primer: BGH_rev
Sequence file: RDB12211_A5Hpc.seq check
>12211_12211_A5Hp_BGHrev_G07_19.ab1
    1 CGTACTCATG GTGATGGTGA TGATGACCGG TATGCATATT CAGATCCTCT TCTGAGATGA
   61 GTTTTTGTTC GAAGGGCCCT CTAGACTCGA GGTACGCCAC AGCGTATGAT GCGTATTTTT
  121 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  181 TTTTTCCTTT CCCAAATTTT TTTTTGGCCC CCTTCAATTA AAAAAAAAAA ATTTTAAGGG
  241 TTTTTGGGGC CCCAAAACCA AGGCGCCCCT TTTAAAACAC CTTCGGCTAA CCCTTTTTTT
  301 TGGGGGGCCC CCCAAAATTT CCCAAAAAAA AGGGAAATTC TTTTTTGGGG GTTTCCTTTG
  361 GCCCCCCCAA AGCCCAAAAA AAACCCCCTT TAAAAAAACC CCCCCCCCCC CCCCCCCCTT
  421 TTTTCCCCCC AAATTAAAAA AAGGAAGGGG AAAAATTTTT AAAAAAACCC TTTGGGAAAC
  481 CCCCCCCCTT TGTTTGGGCT TTTAAAAAAA AAATTCCCCC CCCCCCCCCA CCGGGCCTTT
  541 CCCCCAAAAT TAAAAGGGGG GTTTTTTTTT CCAAAGGGGG GGAGGGGGAG CCGGGGGCAC
  601 CGGGTCAACC GGGTTGACAT ACATAGGGGA AACCAGGGGG GTGGGCGGGA CTGGCCGGGG
  661 TTTCCGACCT CGTGGACCAA GTTGAGTTGA GTGAACGAAC AGGAGGTCCT CCGACCTGAT
  721 TTATTCTTAT TCGAGTCGAC AATCCTGACC GTTGTGGACG ATCACTGGAT GACCGACCAG
  781 TCTGACACTG GAATCAATGG CCCCCCCCCA GTACCATAAT CTGCACACAC GAATACTGCC
  841 ATGGAACTTA CGATGAGTGC GCGTTACGAC GGCGTGAACT GACCTGGATC ACGTGACCCG
901 GAGCGTGGAA AATAATTCGG AACCT
//
Primer: SV40pro_F
Sequence file: RDB12211_A5Hpd.seq check
>12211_12211_A5Hp_SV40pro_F_H07_22.ab1
    1 CCCCTGGTCC TGGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
  661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG CGAAATGACC GACCAAGCGA
  961 CGCCCAACCC TGCCATCACG AGATTTCGAT TCACCGCGCT TCTATGAAAG GTTGGCTTCG
 1021 GATCGTTTTC CGGGACGCCG GCTGATGATC TCAGCGCGGG GGATCTCATG CTTGGAGTCT
 1081 CGCCCACCCC GACTGTATGC AGCTATATGT ACCATTAAGC ATTAGCAATC CACAAACTTT
1141 TCCACAAAAT TAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]