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HA-Amot-delta(101-141)/pcDNA3.1-pA83 (#RDB12212)

Expression clone of mouse Amot (p130), lacking 101-141aa

Clone info. Expression clone of mouse Amot (p130), lacking amino acid residue 101-141. HA-tag.
Comment See Fig. 6D of Hirate et al., Curr. Biol. 23: 1181-1194 (2013). The expression vector has a stretch of poly(A).
Vector backbone pcDNA3.1-pA83 (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name Mouse Amot cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12212 HA-Amot-delta(101-141)/pcDNA3.1-pA83 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The HA-Amot-delta(101-141)/pcDNA3.1-pA83 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12212).

Reference section:

Hirate, Y., Hirahara, S,. Inoue, K., Suzuki, A., Alarcon, V.B., Akimoto, K., Hirai, T., Hara, T., Adachi, M., Chida, K., Ohno, S., Marikawa, Y., Nakao, K., Shimono, A., Sasaki, H., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12212_A5Hpp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB12212_A5Hpa.seq check
>12212_12212_A5Hp_CMV-Forward_G03_21.ab1
    1 AGTATTAACA TCCCGCCCCA ATTGACGCAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA
  121 ATACGACTCA CTATAGGGAG ACCCAAGCTG GCTAGTTAAG CTAGCTTGCC GCCACCATGT
  181 ACCCATACGA CGTTCCAGAT TACGCTGGAT CCAGAAGTTC TGACGATCAG CCAAGTGGAG
  241 GAACGACGGT ACTGCAACGG CTGCTTCAAG AGCAGCTTCG CTATGGCAAT CCCAGTGAGA
  301 ATCGCAGTCT GCTTGCCATA CACCAGCAAG CCACAGGGAA TAGCTCTCCA TTTTCCACGG
  361 GCAGTGGGAA CCAAGGCCCT CAGAATGATG TATTGAGTTC CCAGGATCAC CACCAGCAGC
  421 AGCTTGTGGC ACACCCTGCT CGCCAAGAGC CCCAGGGTCA GGAAATCCAG TCAGAAAACG
  481 GCGTCATGGA GAAGCAGCTG TCCCCTCGAG GATCCATGAG AACCGAGGGA CGCCCGTCAG
  541 TCCAGCGCCT CACGCCCGGG AAGATGCACC AAGATGAAGG CCTTCGAGAT CTTAAGCAAG
  601 GGCATGTCCG ATCCTTGAGT GAAAGACTGA TGCAGATGTC ATTGGCTACT AGTGGAGTTA
  661 AAGCCCACCC ACCTGTTACC AGTGCTCCTC TCTCCCCACC ACAACCCAAT GATCTCTACA
  721 AGAATGCCAC CAGTTCCAGT GAATTCTACA AGGCCCAAGG GCCACCTCCA AGCCAACATA
  781 GTCTGAAGGG CATGGAACAC CGAGGTCCCC CGCCAGAATA CCCTTTCAAG GGCGTGCCAT
  841 CCCAGTCTGT AGTGTGTAAG TCCCAAGAGC CAGGGCACTT CTATAGTGAG CATCGCCTGA
  901 ACCAGCCAGG GAGAACAGAG GGGCAACTGA TGAGGTATCA GCACCCCCCC TGAGTATGGG
  961 GCAGCCAGGG CAACACAGAC ATCTCCTTCG CTGTCTTTGG TCAGCCAGGA ACTCTCAACT
 1021 CATAGCCCTA CTTCTTCCCT GACAGCTTGG GGCTTTCAGC TTGCCTTTGC TGCAGGTCTC
 1081 CGGCGTCCAC TAGAATTGCC CTCCTTGATC ACCACTGATC CCGAAACGAA GGTGAACACT
1141 CTGCTTCACT GCTCAGACAA TCAGCAGCAT TCCTTCCTTC CCGAGA
//
Primer: GNP_primer#10
Sequence file: RDB12212_A5Hpb.seq check
>12212_12212_A5Hp_GNP-primer#10_B08_05.ab1
    1 TTAATTTGTG CAATTCATTT AAAAGATAAC GTTTACAGGT TCTTTGGTGC CCATAGTCGA
   61 GCGGCCAGCT TTAGAGCACA TTCTGCTTAA CCTATCTTTT TGAGGTACTC CCTAAGTATT
  121 CCAAAATAGA TTGGAATTCG TTTCTATGGT ATTACTTATG TACTACCAGA GTCCAATAAA
  181 AAGTCCCTTT AGAAAAATCA TCCCCACCTC CCCACCCTAT CTTCACCCCA GATTAAACAA
  241 ATGAAAGGGA AAAATTATTA AAAAGAACTC TTGCTGATAA CGCCAGCTCT TGATTGTGCT
  301 GTTTAGATGA GATATTCCAC CATCTCTGCA TCAGGCTCTT GCCCCAGGAT CTGAATGGGT
  361 GTCTTTCTTT CCAGAGTGCT GGAGTGGAAA GCAGGGGCAT CTGGCTTATC TGGGTTGCAC
  421 ATCAGATTTG GTGTAGACAA GCGGCGTGGT CCGGAACTGG CACCTGGATT TGCAGGACCC
  481 TCAGTTTGAA CCAAAGCTGG AGTTGGAGTT GGAACTGGAC TAGGAGGTTC AGTCGGAGCT
  541 GGAGTTGATG CCTGAGTCGG AGTCGGAGCA GAAGCCTGAG CCGTTGCTGG AGCCGGAATC
  601 GAAGCTGGAG ATGGAACCGG AGCCGAAGTA GCTGGAGCAA CCTGAATAGC AGTAGTGGCA
  661 GCAGCAGCAG CAGCAGTGAT AGCAGCAGTA GTAGCTGCAG CAACAGCAGC AGTAGCAGTG
  721 GCAGCAGTTG GAGAAACAGT AGCAGGAGTA AGAGATGCAG CAGCTGGAAT CTGAACAGCA
  781 GCAGCTGGAG AGACAGGAGC AGCTGGAGGC ACTACAGCAG CTGGAGCGAC AGGAGCAGCT
  841 GGAGAGATAG AATTAGCTGC AGAGACAGAA GTAGCTGCAG AGACAGAAGT AGCTGGAGCG
  901 GCAGCAGCGG CAAGAGCAGC AGCGTTTGCT GGAGATGGGG TGGCCGGCAG CAGCAGCTGG
  961 GACCAGCACA GCACTGGGAG CAGCAGCTTA CATGATAGCT GTTGTCGCCG CACCAGCGGC
1021 AGTGGTGGGT GGAAGCACTC GCATCGATGA TATTAT
//
Primer: BGH_rev
Sequence file: RDB12212_A5Hpc.seq check
>12212_12212_A5Hp_BGHrev_C08_08.ab1
    1 CGTACTCATG GTGATGGTGA TGATGACCGG TATGCATATT CAGATCCTCT TCTGAGATGA
   61 GTTTTTGTTC GAAGGGCCCT CTAGACTCGA GGTACGCCAC AGCGTATGAT GCGTATTTTT
  121 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  181 TTTTTTTCCC TTCCCCAACT TTTTTTTGGG CCCCCTCCAT TTAAAAAAAA AAATTTTTAG
  241 GGGTTTTTGG GGCCCCAAAC CCAGGCCCCC CTTTTTAAAC CCTTTTTTTT TTACCTTTTT
  301 TTTTGGGGGC CCCCCCAAAA TTCCCCAAAA AAAAGGGAAA CCCCTTCCTG GGGTTTCCCT
  361 TTGGCCCCCC CAAACCCAAA AAAAAACCCC TTTAAAAAAA AACCCCCCCC CCCCCCCCCC
  421 CTTTTTCCCC CCCAAATAAA AAAAGGGAGG GGAAAAATTT TTAAAAAAAA CCCTTGGGAA
  481 AAACCCCCCC CCTTGGTTGG GGGTTTTAAA AAAAAAATTC CCCCCCTCCC GGCAAAGGGT
  541 TTTGCCCCAA AAATTAAAGG GGGGTTTTTT TTTCCCAAAG GGGGGGAGGG AACCGGGGGC
  601 TCTCGGTTAA ACCGGGTTTC ACACATTTTT GGGAAACAGG GCGTTGTGGC GGGAGGGGCC
  661 GGGATTTCAG AACCTCGTTG ACCAAGTGAG ATGAGTTGGA ACTGACTAGA GTTCTCCACT
  721 GAATTATCTG ATCGATCGGA CAACCGACCG TGTGACGATC AGTGGATGAC CGACAATCTG
  781 ACACTGTACC ATTGCACACA CACCGGGTAG CCAATATCGC ACACACTATA CCTGCCATGA
  841 ACATCAGATA GGACCTGTAC ACCCCCCCTG ACGGGCTTGG ACTACTTGTC ACAGCCTGAT
901 ATTGCGGACA GT
//
Primer: SV40pro_F
Sequence file: RDB12212_A5Hpd.seq check
>12212_12212_A5Hp_SV40pro_F_D08_11.ab1
    1 CCCTGGTCTT TACATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
   61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG
  121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG
  661 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  781 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  841 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  901 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGC GAAATGACCG ACCAAGCGAC
  961 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTTCTATGAA AGGGTTGGGC
 1021 TTCGGAATCG TTTTCCGGGA CGCCGGGCTG GATGATCCTC AGCGCGGGGA TCTCATGCTG
 1081 GAGGTCTCCG CCACCCCAAC TGCTTATTGC AGCTATATGC TACCAATAAG GCATTAGCCA
1141 TCACAATTTC CACAAAATAA AGGCAAGTT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]