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pCMV/SV-Flag-Lats2-KD (#RDB12214)

Expression clone of mouse Lats2, kinase dead variant

Clone info. Expression clone of mouse Lats2 cDNA. Catalytically inactive (kinase dead) variant. cDNA fragment 437 to 3,565 nt of NM_015771.2 (CDS, 437..3565) is cloned. A to C substitution at 2,736 nt (Asp to Ala at 767th aa, introduced mutation).
Comment Known differences: C to T substitution at 3457 nt (Asn at 1007th aa, synonymous).
Vector backbone pCMV/SV-Flag1 (Plasmid)
Size of vector backbone 4.6 kb
Gene/insert name Mouse Lats2 cDNA
Depositor|Developer Sasaki, Hiroshi |
 
Sequence (full) RDB12214hts01.seq checkAssembled from experimentally sequenced data.
Publication Hirate, Y., Current Biology 23, 1181-1194 (2013). [link to RRC of NBRP]
Test sheet RDB12214_A6Ajp1-1.pdf 
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12214 pCMV/SV-Flag-Lats2-KD DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pCMV/SV-Flag-Lats2-KD was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12214).

Reference section:

Hirate, Y., Hirahara, S,. Inoue, K., Suzuki, A., Alarcon, V.B., Akimoto, K., Hirai, T., Hara, T., Adachi, M., Chida, K., Ohno, S., Marikawa, Y., Nakao, K., Shimono, A., Sasaki, H., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731. [link to RRC of NBRP]