icon below as well as clone information before placing your order.
Plasmid clone for quantitative assay for genome editing
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Other clones in our bank
External Database E. coli lacZ Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the RECIPIENT must cite the following literature (Biology Open, 2, 363-367 (2013)). |
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| Ordering | Order form [Credit Card Payment  ] [Bank Transfer Payment]Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word ]Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
[open/close]| 提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Biology Open, 2, 363-367 (2013))。 |
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| 提供依頼 | 提供依頼書 [Word ]専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB12261 | pBR-lacZ alpha | DNA solution | JPY 9,460 plus cost of shipping containers, dry ice (if required) and shipping charge |
icon below as well as clone information before placing your order.Materials & Methods section:
| The pBR-lacZ alpha was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12261). |
Reference section:
| Hisano, Y., Ota, S., Arakawa, K., Muraki, M., Kono, N., Oshita, K., Sakuma, T., Tomita, M., Yamamoto, T., Okada, Y., Kawahara, A., Quantitative assay for TALEN activity at endogenous genomic loci. Biology Open., 2: 363-367 (2013). PMID 23616919. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Genome Editing (English text) |
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| Featured content | Genome Editing (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB12261_A6Hup1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: T7_20bp Sequence file: RDB12261_A6Hua.seq
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>12261_12261_A6Hu_1_T7_20bp_D08_11_ABI24.ab1 1 CGTAACATGG GCGCGTCGAG TCGGCGGTAT CGATAAGCGT GTGATATCGA GATTCCTGCA 61 GCCCGGGGGA TCCACTAGTT CTAGAGCGGC CGCCACCGCG GTGGAGCTCC AGCTTTTGTT 121 CCCTTTAGTG AGGGTTAATT TCGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 181 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 241 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 301 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC TAGCAGCACG CCATAGTGAC TGGCGATGCT 361 GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC 421 TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA 481 CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT 541 GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT 601 TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA 661 ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA 721 TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC 781 AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC 841 ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT 901 ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC CGAAAGAACG 961 TTTTTCCCAA TGATGAGCAC TTTTTAAAGT TTCTGCTATG TGGGCGCGGG TATTTATCCC 1021 GTGTTTGACG CCGGGCAAGA GCAACTCGGT TCGGCGCATA CACTACCTCA GATTGACCTT 1081 GGTGAAGTAC CTCACCCAGG TTCCCACAGG A // |
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Primer: T3 Sequence file: RDB12261_A6Hub.seq
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>12261_12261_A6Hu_1_T3_G08_20_ABI24.ab1 1 CAGGCTCACG CGGTGGCGGC CGCTCTAGAC TAGTGGATCC CCCGGGCTGC AGGAATTCGA 61 TATCAAGCTT ATCGATACCG TCGACCTCGA GGGGGGGCCC GGTACCCAAT TCGCCCTATA 121 GTGAGTCGTA TTACAATTCA CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG 181 GCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG 241 AAGAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGGACG 301 CGCCCTGTAG CGAGATCCTC TACGCCGGAC GCATCGTGGC CGGCATCACC GGCGCCACAG 361 GTGCGGTTGC TGGCGCCTAT ATCGCCGACA TCACCGATGG GGAAGATCGG GCTCGCCACT 421 TCGGGCTCAT GAGCGCTTGT TTCGGCGTGG GTATGGTGGC AGGCCCCGTG GCCGGGGGAC 481 TGTTGGGCGC CATCTCCTTG CATGCACCAT TCCTTGCGGC GGCGGTGCTC AACGGCCTCA 541 ACCTACTACT GGGCTGCTTC CTAATGCAGG AGTCGCATAA GGGAGAGCGT CGACCGATGC 601 CCTTGAGAGC CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG ACTATCGTCG 661 CCGCACTTAT GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG GCAGCGCTCT 721 GGGTCATTTT CGGCGAGGAC CGCTTTCGCT GGAGCGCGAC GATGATCGGC CTGTCGCTTG 781 CGGTATTCGG AATCTTGCAC GCCCTCGCTC AAGCCTTCGT CACTGGTCCC GCCACCAAAC 841 GTTTCGGCGA GAAGCAGGCC ATTATCGCCG GCATGGCGGC CGACGCGCTG GGCTACGTCT 901 TGCTGGCGTT CGCGACGCGA GGCTGGATGG CCTTCCCCCA TTATGATTCT TCTCGCTTTC 961 CCGGCGGCAT CGGGATGCCC GCGTTTGCAG CCCATGCTGT CCAGGGCAGT AGATGACGAA 1021 CCAATCAGGA CAGGCCTCAA TGATCGCCTC CGCGCCTCTA CAGTCTACCT TTCGAATCAT 1081 TGGCAAACCG CCTGG // |
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Primer: ColE1ori_F Sequence file: RDB12261_A6Huc.seq
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>12261_12261_A6Hu_1_ColE1ori_F_B09_06_ABI24.ab1 1 TTCTTTTATC TGATCGTCGC ACCGTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG 61 GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT 121 GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA 181 TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT 241 CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG 301 GTATTTCACA CCGCATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA 361 GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC 421 AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC 481 TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC 541 GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG 601 TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA 661 GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG 721 ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGG 781 TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT 841 GGATGCGGCG GGACCAGAGA AAAATCACTC AGGTCAATGC CAGCGCTTCG TTTAATACAG 901 ATGTAAGTGT TCCACAGGGT AGCCCAGCAG CATCCTGCGA TGCAGATCCG GGAACATATG 961 GGTGCAGGCG CTGAACTTTC GGCGTTTCAG ACTTTACGGA AACACGGAAC GAGGACATTC 1021 ATGTTGGTGC TCAGTTCGCA GACGTTTTGC AGCAGCAGCT CGCTCAACGG ATCGCTTCGG 1081 CA // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Hisano, Y., Quantitative assay for TALEN activity at endogenous genomic loci. Biology Open., 2: 363-367 (2013). PMID 23616919. [link to RRC of NBRP] |
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| reference | Hisano, Y., Genome editing using artificial site-specific nucleases in zebrafish. Dev. Growth Differ. 56 (1): 26-33 (2014). PMID 24117409. |
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2023.08.18
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