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pcDNA3Flag-hp38gamma (#RDB12361)

Expression vector of human p38gamma MAPK cDNA, FLAG-tagged

Clone info. Expression vector of human p38gamma MAPK cDNA, FLAG-tagged.
Comment This clone contains a cDNA fragment corresponding to the 175nt to 1278nt of NM_002969.6. Known differences (at least) : T to C substitution at 357nt (61a.a. Pro, synonym) ; T to C substitution at 807nt (211a.a. Ser, syonym).
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Gene/insert name Human p38gamma cDNA
Depositor|Developer Sudo, Tatsuhiko |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. Research purpose only.
提供案内 (日本国内) [open/close]

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12361 pcDNA3Flag-hp38gamma DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3Flag-hp38gamma was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12361).

Reference section:

Kondoh, Y., Honda, K., Hiranuma, S., Hayashi, T., Shimizu, T., Watanabe, N., Osada, H., Comparative chemical array screening for p38gamma/delta MAPK inhibitors using a single gatekeeper residue difference between p38alpha/beta and p38gamma/delta. Sci. Rep. 6: 29881 (2016). PMID 27431267. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12361_A4B5p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert 5'(FLAG,p38 gamma 5')
Sequence file: RDB12361_A4B5a.seq check
>12361_12361_B2A7F_2_CMV-Forward_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNCNNNTGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
  121 CGACTCACTA TAGGGAGACC CAAGCTTGCC ACCATGGACT ACAAGGACGA CGACGATAAA
  181 TCGATGAGCT CTCCGCCGCC CGCCCGCAGT GGCTTTTACC GCCAGGAGGT GACCAAGACG
  241 GCCTGGGAGG TGCGCGCCGT GTACCGGGAC CTGCAGCCCG TGGGCTCGGG CGCCTACGGC
  301 GCGGTGTGCT CGGCCGTGGA CGGCCGCACC GGCGCTAAGG TGGCCATCAA GAAGCTGTAT
  361 CGGCCCTTCC AGTCCGAGCT GTTCGCCAAG CGCGCCTACC GCGAGCTGCG CCTGCTCAAG
  421 CACATGCGCC ACGAGAACGT GATCGGGCTG CTGGACGTAT TCACTCCTGA TGAGACCCTG
  481 GATGACTTCA CGGACTTTTA CCTGGTGATG CCGTTCATGG GCACCGACCT GGGCAAGCTC
  541 ATGAAACATG AGAAGCTAGG CGAGGACCGG ATCCNGTTCC TCGTGTACCA GATGCTGAAG
  601 GGGCTGANGT ATATCNNCGC TGCCNGCATC ATCCNCAGAG ACCTGAAGCC CGGCAACNTG
661 GNTGNGAACN AANACTGNGA NCTGNNNNNC CNNNNNTTNN NCNNNNNNNN NN
//
Primer: bGH_rev3 (Pr0885)
Region: insert 3'(p38 gamma 3')
Sequence file: RDB12361_A4B5b.seq check
>12361_12361_B2A7F_2_bGH_rev3_B02_05_ABI24.ab1
    1 NNNNNNNNNN GNNNNNNNNN NNNCTNNAAN GNGNNNNNNC GAGGNCTGAT CNGCGAGCTC
   61 TAGCATTTAG GTGACACTAT AGAATAGGGC CCTCTAGATC ACAGAGGCGT CTCCTTGGAG
  121 ACCCTGGCCC CCAGCTGCCG GGGAGGCTTG AAGCTGAGCA CCTCTTTGTA AGTAACACGC
  181 TTCCATTCAT CCAGTGTGCG GTCAACGTCG TCAAAGGAGT CATCATACTT CTGGACCTGG
  241 GGCTCATCTT CCGTGTCGTG CAGGGACTCG AAGTAGGGAT GGGCCAGCGC CTCGCCTGCC
  301 GTCACCCGCT GCTCCGCGTC CAGCACCAGC ATCTTCTCCA GGAGGTTCAC AGCCAGAGGG
  361 CTTGCATTGG TCAGGATAGA GGCAAAATCC TTCTTCTCCA ATTCGGGGAG GCCCTTCATG
  421 TAGTTCTTGG CCTCATCGCT CTGCAGCCGC TGCACAAACT CAGCCGGAGG CGTCCCCNNN
  481 NNNTTCATGA TNNNNNNCAG CTGGNCNNNN NGGTCGCTGC CCCTTGANNN NCGTCTTGNN
  541 NGNGATNNNN NCCNCCCNTG ATGCAGCCCA CGGANCNANA NNGNCCNNCG NCTGCNNNGN
  601 ANNNNNNTCC NATTNNNNAT GANNNTNNNG NNCNCGGTAN NNNNNNNNTN NNNCNCNNNN
661 NNAGTCNNNN NNNNNGNNNN GNCNGNNNGG NNNNNNNNNN
//
Primer: p38gamma-M_R (Pr1102)
Region: insert mid(p38 gamma mid)
Sequence file: RDB12361_A4B5c.seq check
>12361_12361_B2A7F_2_p38gamma-M_R_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNCNNNT CTGNNGCCGC TGCACNAACT CNGCCNNGNN NNNNCCCCGT
   61 CACCTTCATG ATCTCCTTCA GCTGGTCCAG GTGGTCGCTG CCCTTGAACA GCGTCTTGCC
  121 TGTGATCATC TCCGCCATGA TGCAGCCCAC GGACCAGATG TCCACCGTCT GCGTGTAGCG
  181 CATCCAATTC AAGATGACCT CGGGAGCCCG GTACCACCGG GTCACCACGT ACCCAGTCAT
  241 CTCACTGTCT GCCTGCCTGG CCAGGCCGAA GTCCAGGATC TTCAGCTCAC AGTCTTCGTT
  301 CACAGCCAGG TTGCCGGGCT TCAGGTCTCT GTGGATGATG CCGGCAGCGT GGATATACCT
  361 CAGCCCCTTC AGCATCTGGT ACACGAGGAA CTGGATCCGG TCCTCGCCTA GCTTCTCATG
  421 TTTCATGAGC TTGCCCAGGT CGGTGCCCAT GAACGGCATC ACCAGGTAAA AGTCCGTGAA
  481 GTCATCCNGG GTCTCATCAG GAGTGAATAC GTCCAGCAGC CCGATCACGT TCTCGTGGCG
  541 CATGTGCTTG AGCAGGCGCA GCTCGCGGTA NGCGCGCTTG GCGAACAGCT CGNACTGGAA
  601 NGGCCGATNC NGCTTCTTGA TGGNCNCCTT ANCNNCNGNG CGGCCNTCCN CNGNCNNANC
661 NCNCCGCNNN NNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR
Sequence file: RDB12361_A4B5d.seq check
>12361_12361_B2A7F_2_SV40pro_ori_F_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNTCTGNNNN NNNCNNGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC
   61 TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA
  121 TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG
  181 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC
  241 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT
  301 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT
  361 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC
  421 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC
  481 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC
  541 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCNGATGG AAGCCGGTCN TGNCGATCAN
  601 GATGNNCTGG ACGANNAGCA TCNNGGGCTN NCGCCNGCCG AANTGNTCGC CNNGCTCNAN
  661 GGNGCGNANG CCCNANNNCN ANNNNCTCNT CNNGACCNNN NGNNNANNNC NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kondoh, Y., Comparative chemical array screening for p38gamma/delta MAPK inhibitors using a single gatekeeper residue difference between p38alpha/beta and p38gamma/delta. Sci. Rep. 6: 29881 (2016). PMID 27431267. [link to RRC of NBRP]