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pcDNA3Flag-hp38delta (#RDB12363)

Expression vector of human p38delta MAPK cDNA, FLAG-tagged

Clone info. Expression vector of human p38delta MAPK cDNA, FLAG-tagged.
Comment This clone contains a cDNA fragment corresponding to the 71nt to 1168nt of NM_002754.5. Known difference (at least) : A to C substitution at 121nt (17a.a. Thr, synonym).
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 ｋｂ
Gene/insert name Human p38delta cDNA
Depositor|Developer Sudo, Tatsuhiko |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. Research purpose only.
提供案内 (日本国内) [open/close]

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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12363 pcDNA3Flag-hp38delta DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3Flag-hp38delta was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12363).

Reference section:

Kondoh, Y., Honda, K., Hiranuma, S., Hayashi, T., Shimizu, T., Watanabe, N., Osada, H., Comparative chemical array screening for p38gamma/delta MAPK inhibitors using a single gatekeeper residue difference between p38alpha/beta and p38gamma/delta. Sci. Rep. 6: 29881 (2016). PMID 27431267. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12363_A4B5p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert 5'(FLAG,p38 delta 5')
Sequence file: RDB12363_A4B5a.seq check
>12363_12363_B2A7F_1_CMV-Forward_A03_03_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNANNNGTG GGNGGTCTAT ATAAGCAGAG CTCTCTGGCT
   61 AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT ACGACTCACT ATAGGGAGAC
  121 CCAAGCTTGC CACCATGGAC TACAAGGACG ACGACGATAA ATCGATGAGC CTCATCCGGA
  181 AAAAGGGCTT CTACAAGCAG GACGTCAACA AGACCGCCTG GGAGCTGCCC AAGACCTACG
  241 TGTCCCCGAC GCACGTCGGC AGCGGGGCCT ATGGCTCCGT GTGCTCGGCC ATCGACAAGC
  301 GGTCAGGGGA GAAGGTGGCC ATCAAGAAGC TGAGCCGACC CTTTCAGTCC GAGATCTTCG
  361 CCAAGCGCGC CTACCGGGAG CTGCTGCTGC TGAAGCACAT GCAGCATGAG AACGTCATTG
  421 GGCTCCTGGA TGTCTTCACC CCAGCCTCCT CCCTGCGCAA CTTCTATGAC TTCTACCTGG
  481 TGATGCCCTT CATGCAGACG GATCTGCAGA AGATCATGGG GATGGAGTTC AGTGAGGAGA
  541 AGATCCAGTA CCTGGTGTAN CAGATGCTNN NNNGCCTTAA GTACATCCAC TCTGCTGNNG
  601 TCGTGCACAN GGNNCTGANN NCAGGNNNNN NGGCTGTGAA TGANGNNTGT GANCTGNNNA
661 NTCTGGNNNN TTTGGGNNNN NNNNNACNNG NNNNNNNNCN NNNNNNNNN
//
Primer: bGH_rev3 (Pr0885)
Region: insert 3'(p38 delta 3')
Sequence file: RDB12363_A4B5b.seq check
>12363_12363_B2A7F_1_bGH_rev3_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNGGNNNN NNNNNNNNNA NNAGNTGGCT GGCAACTAGN NAGGCACAGT
   61 CGAGGCTGAT CAGCGAGCTC TAGCATTTAG GTGACACTAT AGAATAGGGC CCTCTANATG
  121 AGTCCCTACA GCTTCATGCC ACTCCGGCGC CGTGAGTCCT TCCGGGCAAT GGGGCTGAAG
  181 TTCACAATCT CCTTGTAGAT GTGCTGCTTC CATTCATCCA CTGTGAGTTT CTCGTGTTCT
  241 AAGGAATCAT CAAACGGCTG CTGGGCCTCC GTCTCTTCCT CAGGGTCCCG GAAGGGTTCA
  301 AAGAAGGGAT GGGTGAGGGC CTGCGCGGCC GTCAGGCGCT TGTCCACGTC TAGCTCCAGC
  361 ATCTTCTCCA GCAGGTCCGC AGCCTGGGGG CTGGCCCGTG GGAACAGCTG AGTGAAATCC
  421 TTCCTGGGGG TCTGTGGCAG GGACTGGATG TAGGATTTGG CCGCTTTGTC GTTCAGCTTC
  481 TGCACAAACT CCGTGCCAGG CACCCCGGTC ACTTTCAGGA TCTGGGTCAG CTGGTCCAGG
  541 TAATCTTTCC CCTTGAACAG AGTTTTCCCT GTCNNCATCT CTGCCATGNN ACAGCCCANA
  601 GACCANANGT CNNCTGTCTG GNTGTAGTGC ATNCNNCTGA GGATCACNTN NNGGGGCTCG
661 GTACNNNNNG GTCACCNNNN NNNCNNNNNN NNNN
//
Primer: p38delta-M_R (Pr1103)
Region: insert mid(p38 delta mid)
Sequence file: RDB12363_A4B5c.seq check
>12363_12363_B2A7F_1_p38delta-M_R_H01_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGCCGCTTT GTCGTTNNGC TTCTGCACAA ACTCCGTGCC
   61 AGGCACCCCG GTCACTTTCA GGATCTGGGT CAGCTGGTCC AGGTAATCTT TCCCCTTGAA
  121 CAGAGTTTTC CCTGTCAGCA TCTCTGCCAT GATACAGCCC ACAGACCAGA TGTCCACTGT
  181 CTGGTTGTAG TGCATCCAGC TGAGGATCAC CTCGGGGGCT CGGTACCAGC GGGTCACCAC
  241 GTAGCCAGTC ATCTCGGCGT CTGCATGTCG CGCCAGCCCA AAATCCAGAA TCTTCAGTTC
  301 ACAGTCCTCA TTCACAGCCA GGTTGCCTGG CTTCAGGTCC CTGTGCACGA CCCCAGCAGA
  361 GTGGATGTAC TTAAGGCCTT TGAGCATCTG ATACACCAGG TACTGGATCT TCTCCTNNNN
  421 NNACTCCATC CCCATGATCT TCNGNNNATC CGTCTGCATG AAGGGCATCA CCANNTANAN
481 NNNATAGAAG TTGCGCNNGG AGGANNNNGG NNNGAANANN NNNANNANNN NNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: Sv40 pro_ori,NeoR
Sequence file: RDB12363_A4B5d.seq check
>12363_12363_B2A7F_1_SV40pro_ori_F_C03_09_ABI24.ab1
    1 NNNNNCNGNN CTATTCNNGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
   61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
  121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCNATC ANGATGATCT
  601 GGACGAAGAG CATCANGGGC TCGCGCCNGC CNAACTGNTC GCCNNGCTNN ANGCGCNNAT
  661 GNCCGANNGC GANNNNCTCN TCNNGACCNN NGGNNNNGNC NGNNNNNNNA NNNNNNNNNG
721 NNNGNNAANN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kondoh, Y., Comparative chemical array screening for p38gamma/delta MAPK inhibitors using a single gatekeeper residue difference between p38alpha/beta and p38gamma/delta. Sci. Rep. 6: 29881 (2016). PMID 27431267. [link to RRC of NBRP]