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pRSET-hp38deltaM107T

Bacterial expression vector of human p38delta, gate keeper mutant, His6-tagged.

Catalog number RDB12369
Resource name pRSET-hp38deltaM107T
Clone info. Bacterial expression vector of human p38delta, His6-tagged. Gate keeper mutant, sensitive to SB203580.
Comment This clone contains a cDNA fragment corresponding to the 71nt to 1198nt of NM_002754.5. Known difference (at least) : A to C substitution at 121nt (17a.a. Thr, synonym).
Vector backbone pRSET C (Plasmid)
Size of vector backbone 2.9 kb
Gene/insert name Human p38delta cDNA
Depositor|Developer Sudo, Tatsuhiko |
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External Database
Human p38delta

          Reference sequence
            
           
          Sequence (full) RDB12369zzt01.seq compiled by us

          Distribution information

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          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. Research purpose only.
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          RDB12369 pRSET-hp38deltaM107T DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pRSET-hp38deltaM107T was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12369).

          Reference section:

          Kondoh, Y., Honda, K., Hiranuma, S., Hayashi, T., Shimizu, T., Watanabe, N., Osada, H., Comparative chemical array screening for p38gamma/delta MAPK inhibitors using a single gatekeeper residue difference between p38alpha/beta and p38gamma/delta. Sci. Rep. 6: 29881 (2016). PMID 27431267.

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content

          Featured content MAPK p38 inhibitor screening (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12369_A4B5p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: T7_20bp (Pr0261)
          Region: 6xHis, T7 tag, Xpress tag, Insert 5'
          Sequence file: RDB12369_A4B5a.seq check
          >RDB12369_1_T7_20mer_E08_14.ab1
              1 NNNNNNNNNN NNNNNNNNNN TTTGNTNACT TTAAGAAGGA NNNATACATA TGCGGGGTTC
             61 TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT GGACAGCAAA TGGGTCGGGA
            121 TCTGTACGAC GATGACGATA AGGATCGATG GATCCCATCG ATGAGCCTCA TCCGGAAAAA
            181 GGGCTTCTAC AAGCAGGACG TCAACAAGAC CGCCTGGGAG CTGCCCAAGA CCTACGTGTC
            241 CCCGACGCAC GTCGGCAGCG GGGCCTATGG CTCCGTGTGC TCGGCCATCG ACAAGCGGTC
            301 AGGGGAGAAG GTGGCCATCA AGAAGCTGAG CCGACCCTTT CAGTCCGAGA TCTTCGCCAA
            361 GCGCGCCTAC CGGGAGCTGC TGCTGCTGAA GCACATGCAG CATGAGAACG TCATTGGGCT
            421 CCTGGATGTC TTCACCCCAG CCTCCTCCCT GCGCAACTTC TATGACTTCT ACCTGGTGAC
            481 GCCCTTCATG CAGACGGATC TGCAGAAGAT CATGGGGATG GAGTTCAGTG AGGAGAAGAT
            541 CCAGTACCTG GTGTATCAGA TGCTCAAAGG CCTTAAGTAC ATCCACTCTG CTGGGGTCGT
            601 GCACAGGGAC CTGAAGCCAG GCAACCTGGC TGTGAATGAG GACTGTGAAC TGAAGATTCT
            661 GGATTTTGGG CTGGCGCGAC ATGCAGACGC CGAGATGACT GGCTACGTGG TGACCCGCTG
            721 GTACCGAGCC CCCGAGGTGA TCCTCAGCTG GATGCACTAC AACCAGACAG TGGACATCTG
            781 GTCTGTGGGC TGTATCATGG CAGAGATGCT GACAGGGAAA ACTCTGTTCN AGGGGAAAGA
            841 TTACCTGNAC NAGCTGANCC AGATCCTGAA AGTGACCGGG GTGCCTGGCA CGGAGTTTGT
            901 GCAGAANNCT GAACGANAAA GCGGCCNAAT CCTACATNNN GTCCCNGCCN CANANCCCCC
          961 NNGANNGANT NCANNCNNCT GNNNCCNCNG NNNNNCCCCC CANNCTGNNN NNNNNNN
          //
          Primer: T7 terminator (Pr0155)
          Region: insert 3'
          Sequence file: RDB12369_A4B5b.seq check
          >RDB12369_1_T7terminator_F08_17.ab1
              1 NNNNNNNNNN NNNNNNNGNT NNGTNNGCNG CCGGNNNNAG CTTCGAATTC NNTGGTACCN
             61 GCTGCAGGGC CCTCTAGATG AGTCCCTACA GCTTCATGCC ACTCCGGCGC CGTGAGTCCT
            121 TCCGGGCAAT GGGGCTGAAG TTCACAATCT CCTTGTAGAT GTGCTGCTTC CATTCATCCA
            181 CTGTGAGTTT CTCGTGTTCT AAGGAATCAT CAAACGGCTG CTGGGCCTCC GTCTCTTCCT
            241 CAGGGTCCCG GAAGGGTTCA AAGAAGGGAT GGGTGAGGGC CTGCGCGGCC GTCAGGCGCT
            301 TGTCCACGTC TAGCTCCAGC ATCTTCTCCA GCAGGTCCGC AGCCTGGGGG CTGGCCCGTG
            361 GGAACAGCTG AGTGAAATCC TTCCTGGGGG TCTGTGGCAG GGACTGGATG TAGGATTTGG
            421 CCGCTTTGTC GTTCAGCTTC TGCACAAACT CCGTGCCAGG CACCCCGGTC ACTTTCAGGA
            481 TCTGGGTCAG CTGGTCCAGG TAATCTTTCC CCTTGAACAG AGTTTTCCCT GTCAGCATCT
            541 CTGCCATGAT ACAGCCCACA GACCAGATGT CCACTGTCTG GTTGTAGTGC ATCCAGCTGA
            601 GGATCACCTC GGGGGCTCGG TACCAGCGGG TCACCACGTA GCCAGTCATC TCGGCGTCTG
            661 CATGTCGCGC CAGCCCAAAA TCCAGAATCT TCAGTTCACA GTCCTCATTC ACAGCCAGGT
            721 TGCCTGGCTT CAGGTCCCTG TGCACGACCC CAGCAGAGTG GANGTACTTA AGGCCTTTGA
            781 GCATCTGATA CACCAGGTAC TGGATCTTCT CCTCACTGAA CTCCNTCCCC ATGATCTTCT
            841 GCAGATCCGT CTGCATGAAN GGCGTCACCA GGTAGAAGTC ATAGAAGTTG CGCANGGNGG
            901 ANGCTGGGNN GAAGACATCC NGGANCCCNA TGNNGTTCTC ATGCTGNATG TGCTTCANCA
            961 NCAGCAGCTC CCNGNANNNN NGCTTGNNNA ANANCTCNGN NTGAAANGGN NNGNNCANCN
          1021 NCNTNNNTNG NNNNNNNNNN
          //
          Primer: p38delta-M_R (Pr1103)
          Region: insert mid (M107T)
          Sequence file: RDB12369_A4B5c.seq check
          >12369_12369_B2AkF_1_p38delta-M_R_C01_03_ABI08.ab1
              1 NNNNNNNNNN TTNGTCGTTN NGCTTCTGCA CAAACTCCGT GCCAGGCACC CCGGTCACTT
             61 TCAGGATCTG GGTCAGCTGG TCCAGGTAAT CTTTCCCCTT GAACAGAGTT TTCCCTGTCA
            121 GCATCTCTGC CATGATACAG CCCACAGACC AGATGTCCAC TGTCTGGTTG TAGTGCATCC
            181 AGCTGAGGAT CACCTCGGGG GCTCGGTACC AGCGGGTCAC CACGTAGCCA GTCATCTCGG
            241 CGTCTGCATG TCGCGCCAGC CCAAAATCCA GAATCTTCAG TTCACAGTCC TCATTCACAG
            301 CCAGGTTGCC TGGCTTCAGG TCCCTGTGCA CGACCCCAGC AGAGTGGATG TACTTAAGGC
            361 CTTTGAGCAT CTGATACACC AGGTACTGGA TCTTCTCCTC ACTGAACTCC ATCCCCATGA
            421 TCTTCTGCAG ATCCGTCTGC ATGAAGGGCG TCACCAGGTA GAAGTCATAG AAGTTGCGCA
            481 GGGAGGAGGC TGGGGTGAAG ACATCCAGGA GCCCAATGAC GTTCTCATGC TGCATGTGCT
            541 TCAGCAGCAG CAGCTCCCGG TAGGCGCGCT TGGCGAANAT CTCGGACTGA AAGGGTCGGC
            601 TCAGCTTCTT GATGGCCACC TTCTCCCCTG ACCGCTTGTC GATGGNCGAN CACACGGANC
            661 NNTNNNNCCN GCTGCCGANG NGCGTCNGGG NNNCGTNNNC NNNNNNNCTN CNNNGCNGNN
          721 NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kondoh, Y., Comparative chemical array screening for p38gamma/delta MAPK inhibitors using a single gatekeeper residue difference between p38alpha/beta and p38gamma/delta. Sci. Rep. 6: 29881 (2016). PMID 27431267.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl