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pRSET-hp38deltaM107T (#RDB12369)

Bacterial expression vector of human p38delta, gate keeper mutant, His6-tagged.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)
GenBank Fla File Format
Publication Kondoh, Y., Sci. Rep. 6: 29881 (2016). PMID 27431267. [link to RRC of NBRP]
Test sheet Data Sheet 
 
Clone info. Bacterial expression vector of human p38delta, His6-tagged. Gate keeper mutant, sensitive to SB203580.
Comment This clone contains a cDNA fragment corresponding to the 71nt to 1198nt of NM_002754.5. Known difference (at least) : A to C substitution at 121nt (17a.a. Thr, synonym).
Vector backbone pRSET C (Plasmid)
Size of vector backbone 2.9 kb
Gene/insert name Human p38delta cDNA
Depositor|Developer Sudo, Tatsuhiko |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. Research purpose only.
提供案内 (日本国内) [open/close]

必要書類
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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Catalog # Resource name Shipping form Fee
RDB12369 pRSET-hp38deltaM107T DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge


How to cite this biological resource

Materials & Methods section:

The pRSET-hp38deltaM107T was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12369).

Reference section:

Kondoh, Y., Honda, K., Hiranuma, S., Hayashi, T., Shimizu, T., Watanabe, N., Osada, H., Comparative chemical array screening for p38gamma/delta MAPK inhibitors using a single gatekeeper residue difference between p38alpha/beta and p38gamma/delta. Sci. Rep. 6: 29881 (2016). PMID 27431267. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Kondoh, Y., Comparative chemical array screening for p38gamma/delta MAPK inhibitors using a single gatekeeper residue difference between p38alpha/beta and p38gamma/delta. Sci. Rep. 6: 29881 (2016). PMID 27431267. [link to RRC of NBRP]