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pFBD-Hs alpha1CGH/beta3CGF (#RDB12753)

Plasmid clone for baculoviral expression of alpha1 and beta3 tubulin


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Remarks, protocol and/or map (pdf) RDB12753z.pdf
Publication Minoura, I., FEBS Lett., 587 (21): 3450-3455 (2013). PMID 24021646. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name FLAG-and His-tagged Human alpha1 beta3 tubulin
Clone info. Plasmid clone for baculoviral expression of alpha1(NP_006073) and beta3 (NP_006077) tubulin.
Comment Human alpha1-tubulin is fused to linker (GGSGG) and His-tag (HHHHHHHH) at its C-terminus. Human b3-tubulin is fused to linker (GGSGG) and FLAG-tag (DYKDDDDK) at its C-terminus.
Vector backbone pFastBac Dual (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r
Gene/insert name Human alpha1-Tb cDNA Human beta3－Tb cDNA
Depositor|Developer Muto, Etsuko |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not- for profit organization for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Minoura, I., FEBS Lett., 587, 3450-3455 (2013)) and an acknowledgment to the DEPOSITOR are requested. The BIOLOGICAL RESOURCE will not be further distributed to others without the DEPOSITOR's written consent. RECIPIENT must contact the DEPOSITOR in the case of application for any patents based on the results from the use of the BIOLOGICAL RESOURCE.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Minoura, I. et al. FEBS Lett. 587, 3450-3455, 2013)を引用し、謝辞の表明を必要とする。本件リソースは学術機関での学術研究への利用に限る。利用者は、寄託者の承諾書なしに第三者への本件リソースの提供を行ってはならない。利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請を行う場合は、受託者と別途協議を行う。

Catalog # Resource name Shipping form Fee
RDB12753 pFBD-Hs alpha1CGH/beta3CGF DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pFBD-Hs alpha1CGH/beta3CGF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12753).

Reference section:

Minoura, I., Hachikubo, Y., Yamakita, Y., Takazaki, H., Ayukawa, R., Uchimura, S., Muto, E., Overexpression, purification, and functional analysis of recombinant human tubulin dimer. FEBS Lett., 587 (21): 3450-3455 (2013). PMID 24021646. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Minoura, I., Overexpression, purification, and functional analysis of recombinant human tubulin dimer. FEBS Lett., 587 (21): 3450-3455 (2013). PMID 24021646. [PubMed] [Article] [RRC of NBRP]