RDB12840 - Histac pcDNA3.1

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

Histac pcDNA3.1 (#RDB12840)

For the detection of acetylation at the 5th and 8th lysine residues of histone H4 by the BRDT bromodomain.

Clone info.	For the detection of acetylation at the 5th and 8th lysine residues of histone H4 by the BRDT bromodomain.
Comment	Expression was confirmed by observation fluorescent in COS7 cells under the microscope by the depositor.
Vector backbone	pcDNA3.1 (Plasmid)
Size of vector backbone	5.5kb
Gene/insert name	Mouse testis-specific bromodomain cDNA Mouse Histone H4 cDNA Aequorea victoria GFP cDNA
Depositor\|Developer	Sasaki, Kazuki \|

Sequence	RDB12840z.seq
Remarks, protocol and/or map (pdf)	RDB12840.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Proc. Natl. Acad. Sci. U.S.A. 106, 16257-16262, 2009). The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Proc. Natl. Acad. Sci. U.S.A. 106, 16257-16262, 2009). The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Proc. Natl. Acad. Sci. U.S.A. 106, 16257-16262, 2009)。本件リソースは学術研究機関のみに提供し、学術研究にのみ利用することができる。付加的使用条件： When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Proc. Natl. Acad. Sci. U.S.A. 106, 16257-16262, 2009)。本件リソースは学術研究機関のみに提供し、学術研究にのみ利用することができる。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12840	Histac pcDNA3.1	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Histac pcDNA3.1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12840).

Reference section:

Sasaki, K., Ito, T., Nishino, N., Khochbin, S., Yoshida, M., Real-time imaging of histone H4 hyperacetylation in living cells. Proc. Natl. Acad. Sci. U.S.A. 106 (38): 16257-16262 (2009). PMID 19805290. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12840_A4Ihp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5'(Venus 5') Sequence file: RDB12840_A4Ihe.seq
>RDB12840_3_CMV_Forward_B11_05.ab1 1 NNNNNNNNNN NNNNNNGNNG CNNATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA 121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGGTA CCATGGTGAG 181 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT 241 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT 301 GACCCTGAAG CTGATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC 361 CACCCTGGGC TACGGCCTGC AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA 421 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA 481 CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG 541 CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA 601 GTACAACTAC AACAGCCACA ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA 661 GGCCAACTTC AAGATCCGCC ACAACATCGA GGACGGCGGC GTGCAGCTCG CCGACCACTA 721 CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG CCCGACAACC ACTACCTGAN 781 CTACCAGTCC GCCCTGANCA AAGACCCCNA CGAGAANCGC GATCACATGG TCCTGCTGGA 841 GTTCGTGANN GCCGCCGGNN TCACTCTCGG CATGGACNAN CTGTACAANG AATTCANGTC 901 TCTGNCNNGN CGANAAACAG CTATNGNTNA CCCCCCNNCN CCANANNANN TAAACNCTAA 961 NAANNNNGGN NNGNTNNNNN ANNCNNCTGC NNNNNNNNNN //
Primer: bGH_rev3 (Pr0885) Region: insert 3'(ECFP 3') Sequence file: RDB12840_A4Ihf.seq
>12840_12840_B2G4F_3_bGH_rev3_E02_14_ABI24.ab1 1 NNNNNNNNNN NNNNGGGGGN NGGNNNNNNA CAGANGGCTG GCAACTAGAA GGCACAGTCG 61 AGGCTGNTCA GCGGGTTTAA ACGGGCCCTC TAGACTCGAG TTACTTGTAC AGCTCGTCCA 121 TGCCGAGAGT GATCCCGGCG GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT 181 CGTTGGGGTC TTTGCTCAGG GCGGACTGGG TGCTCAGGTA GTGGTTGTCG GGCAGCAGCA 241 CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCTGCCGT 301 CCTCGATGTT GTGGCGGATC TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG 361 TGATATAGAC GTTGTGGCTG ATGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT 421 CCTCCTTGAA GTCGATGCCC TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT 481 TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA GAAGATGGTG CGCTCCTGGA 541 CGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC 601 GGCTGAAGCA CTGCACGCCC CAGGTCAGGG TGGTCACGAG GGTGGGCCAG GGCACGGGCA 661 GCTTGCCGGT GGTGCAGATG AACTTCAGGG TCAGCTTGCC GTANGTGGCA TCGCCCTCGC 721 CCTCGCCGGA CACGCTGAAC TTGTGGCCGT TTACGTCGCC GTCCAGCTCG ACCAGGATGG 781 GCACCACCCC GGTGAACAGC TCCTCGCCCT TGCTCACCAT GNTATCAACC ACTTTGTACA 841 AGAAAGCTGG GTCGACGCCG CCNAAGCCGT ACAGANTGCG GCCCTGGCGC TNGAGCGCGT 901 ACACCACNNC CATAGCCGNG NCCGNCTTGC NCTNNGCGNG NNCNGNNNNN NGACNGCNNN 961 NNGGNTNNCN TTCNCNNNNA AAANNNNNAN GNNNNNNNNN N //
Primer: mBrdt-H_R (Pr1233) Region: insert mid(mouse Brdt 5',Venus 3') Sequence file: RDB12840_A4Ihg.seq
>12840_12840_B2G4F_3_mBrdt-H_R_H04_22_ABI24.ab1 1 NNNNNNNNNN NNNNNGCTTC NNNNGGNNCT TNNGCACNAC CCTCTGTAGG AACTGCAGCT 61 GATTCGTCAA CCGCCCACTC TTCTTAGTGT TTATGTACTC TGGTGGAGGG GGGTTAACAA 121 TAGCTGTTTG TCGACTTGGC AGAGACATGA ATTCCTTGTA CAGCTCGTCC ATGCCGAGAG 181 TGATCCCGGC GGCGGTCACG AACTCCAGCA GGACCATGTG ATCGCGCTTC TCGTTGGGGT 241 CTTTGCTCAG GGCGGACTGG TAGCTCAGGT AGTGGTTGTC GGGCAGCAGC ACGGGGCCGT 301 CGCCGATGGG GGTGTTCTGC TGGTAGTGGT CGGCGAGCTG CACGCCGCCG TCCTCGATGT 361 TGTGGCGGAT CTTGAAGTTG GCCTTGATGC CGTTCTTCTG CTTGTCGGCG GTGATATAGA 421 CGTTGTGGCT GTTGTAGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA 481 AGTCGATGCC CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG 541 CGCGGGTCTT GTAGTTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ACGTAGCCTT 601 CGGGCATGGC GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGGCGAAGC 661 ACTGCAGGCC GTAGCCCAGG GTGGTCACGA GGGTGGGCCA GGGCACGGGC AGCTTGCCGG 721 TGGTGCAGAT CAGCTTCAGG GTCAGCTTGC CGTANGTGGC ATCGCCCTCG CCCTCGCCGG 781 ACACGCTGAA CTTGTGGCCG TTTACGTCGC CGTCCAGCTC GACCAGGNTG GGCACCACCC 841 CGGTGAACAG CTCCTCGCCC TTGCTCANCA TGGTANCNAG CTTNAGTTTA AACGCTAGCC 901 AGCTNGGGTC TCCNNANANN GNGTCGNANT NNTTNNNNNN NNCCAGTNAG CANNNGGNTC 961 NNNANNNNNN NNNN //
Primer: mCerulean-N_R (Pr0770) Region: insert mid(ECFP 5',attB2, mouse H4 histone 16,mouse Brdt 3') Sequence file: RDB12840_A4Ihh.seq
>12840_12840_B2G4F_3_mCerulean-N_R_F02_17_ABI24.ab1 1 NNNNNNNNNN NNNNNGNNNN GGNNNCTNGC CGGNGGTGCA GANGAACTTC AGGGTCAGCT 61 TGCCGTAGGT GGCATCGCCC TCGCCCTCGC CGGACACGCT GAACTTGTGG CCGTTTACGT 121 CGCCGTCCAG CTCGACCAGG ATGGGCACCA CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA 181 CCATGATATC AACCACTTTG TACAAGAAAG CTGGGTCGAC GCCGCCGAAG CCGTACAGAG 241 TGCGGCCCTG GCGCTTGAGC GCGTACACCA CGTCCATAGC CGTGACCGTC TTGCGCTTGG 301 CGTGCTCGGT GTAGGTGACG GCGTCGCGGA TCACATTCTC AAGGAAAACC TTGAGGACAC 361 CGCGAGTCTC CTCGTAGATG AGACCCGAGA TGCGCTTCAC GCCGCCACGC CGAGCCAACC 421 GCCGGATAGC GGGCTTGGTA ATGCCCTGGA TGTTGTCGCG GAGAACCTTG CGGTGTCGCT 481 TGGCGCCACC TTTCCCCAGA CCCTTGCCGC CTTTTCCTCG TCCTGACATG GATCCAGATC 541 CTCCACCACC TGAACCACCA CCTCCAGAAC CTCCTCCACC AGATCCACCT CCTCCGTCGA 601 CTGGAACTTG GGACAGAACT TGCAGCTGCT GATGAACAGC ATTAAGCTGC TCCTGAAGCT 661 TGGCAAGATG CTGCACTCGT TCATCCTCAG AGTCTTCAGA AGAGGCGTCC CCCGANGANG 721 CCTCGCTGCT GCTTTCTCTG CTGANGGCTT GTGCACTGTT TGTTGTAAGG TGGCATGCAT 781 GCATACTCTC AATANGTTCA TCGGGAATCT TGGCAAAATG TANTTCAAAA ACATCCTGAA 841 NGGTCCNANC CATCGCCNCA ACTTCNTGAT CGGGNNGNNT ATACTTGTAA CAGTTCATGA 901 ACATCANTCN GANNTCTGCA NCAAATTNAT ATGCNTCCNT ANANTNNNGA NNGNCCNTTT 961 TCCCNNNNNT CNNNNNNNNN N //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR Sequence file: RDB12840_A4Ihi.seq
>12840_12840_B2G4F_3_SV40pro_ori_F_G02_20_ABI24.ab1 1 NNNNNNNNNN NNGNNGCCTC TGCCTCTGAN CTATTCNNGA AGTAGTGAGG AGGCTTTTTT 61 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 121 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 181 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC 241 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC 301 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC 361 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 421 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 481 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC 541 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT 601 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC 661 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC 721 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 781 CTGGGTGTGG CGGANCGCTA TCANGACATA GCGTTGGCTA CNNGTGNNAT TGCTGAAGAG 841 CTTGGCGGCG AATGGGCTGA CCGCTTCCTC NNGCTTTACN GTATCGCCGC TCCCGANTCG 901 CAGCGCATCG CCNTCNNTCG CNNTCTTGAC NANTTCNTCN NANCGGNNNN NNNGGGGTTC 961 NAAANGNNNN ANNNNNCNNN NNCNNNNCNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Sasaki, K., Real-time imaging of histone H4 hyperacetylation in living cells. Proc. Natl. Acad. Sci. U.S.A. 106 (38): 16257-16262 (2009). PMID 19805290. [link to RRC of NBRP]
reference	He, M., Chemical Biology Approaches for Investigating the Functions of Lysine Acetyltransferases. Angew. Chem. Int. Ed. Engl. 57 (5): 1162-1184 (2018). PMID 28786225.
reference	Soleja, N., Role of green fluorescent proteins and their variants in development of FRET-based sensors. J. Biosci. 43 (4): 763-784 (2018). PMID 30207321.
user_report	Santoni, G., Chromatin plasticity predetermines neuronal eligibility for memory trace formation. Science 385 (6707): eadg9982 (2024). PMID 39052786. [link to RRC of NBRP]
user_report	Dancy, B.M., Live-cell studies of p300/CBP histone acetyltransferase activity and inhibition. Chembiochem. 13 (14): 2113-2121 (2012). PMID 22961914. [link to RRC of NBRP]