RDB12867 - CS-EVBsd

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

CS-EVBsd (#RDB12867)

Lentivirus vector plasmid of Venus fluorescent protein and BSD tethered with T2A driven by EF-1 alpha promoter.

Clone info.	Lentivirus vector plasmid of Venus fluorescent protein and BSD tethered with T2A driven by EF-1 alpha promoter.
Comment	There are attB1 and attB2 recombination sites upstream of the EF-1 alpha promoter. Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone	Plasmid
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth conditions	37C, LB+Amp
Depositor\|Developer	Miyoshi, Hiroyuki \|

Sequence (full)	RDB12867zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Remarks Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks	Remember that you will be working with samples containing infectious virus.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。付加的使用条件： When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12867	CS-EVBsd	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CS-EVBsd was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12867).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12867_B2Abp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF-1 alpha pro,Venus 5' Sequence file: RDB12867_B2Aba.seq
>12867_12867_B2Ab_3_EF1a-C_F_D01_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNTNTT NAGTTTGGNN CTTGGTTNNT TCTCNAGCCT CAGACAGTGG 61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAATTAA TTAACCGGTT CCACAACCAT 121 GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG 181 CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG 241 CAAGCTGACC CTGAAGCTGA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT 301 CGTGACCACC CTGGGCTACG GCCTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA 361 GCACGACTTC TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT 421 CAAGGACGAC GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT 481 GAACCGCATC GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA 541 GCTGGAGTAC AACTACAACA GCCACAACGT CTATATCACC GCCGACNAGC AGAAGAACGG 601 CATCNAGGCC AACTTCNAGA TCCGCCACNA CATCGAGGAN GGCNGCGTGC AGCTCGCCGA 661 NCACTACCAG CAGAACACCC CCNTCNGCGA CNGCCCNNNG CTGCTGNCCN NNNACCNCNA 721 NNNNNNNNNN //
Primer: PRE_rev (Pr0250) Region: PRE,BSD,T2A,Venus 3' Sequence file: RDB12867_B2Abb.seq
>12867_12867_B2Ab_3_PRE_rev_B01_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NAAANNNNNN NNNNNNNNNT AAGAATACCN GTCAATCTTT 61 CNCAAATTTT GTAATCCAGA GGTTGATTAT CGATAAGCTT GATCCCTCGA GTGCGGCCTT 121 AGCCCTCCCA CACATAACCA GAGGGCAGCA ATTCACGAAT CCCAACTGCC GTCGGCTGTC 181 CATCACTGTC CTTCACTATG GCTTTGATCC CAGGATGCAG ATCGAGAAGC ACCTGTCGGC 241 ACCGTCCGCA GGGGCTCAAG ATGCCCCTGT TCTCATTTCC GATCGCGACG ATACAAGTCA 301 GGTTGCCAGC TGCCGCAGCA GCAGCAGTGC CCAGCACCAC GAGTTCTGCA CAAGGTCCCC 361 CAGTAAAATG ATATACATTG ACACCAGTGA AGATGCGGCC GTCGCTAGAG AGAGCTGCGC 421 TGGCGACGCT GTAGTCTTCA GAGATGGGGA TGCTGTTGAT TGTAGCCGTT GCTCTTTCAA 481 TGAGGGTGGA TTCTTCTTGA GACAAAGGCT TGGCCATGGG CCCGGGATTT TCCTCCACGT 541 CACCGCATGT TAGAAGACTT CCTCTGCCCT CGCCGGATCC CTTGTACAGC TCGTCCATGC 601 CGAGAGTGAT CCCNGCGGCG GTCACNAACT CNNGCNNGAC CNTGTGATCG CGCTTCTCNT 661 NGGGGTCTTT GCTCNNNNNN NACNGGNANC TCNNNNNNNN NGNCNGNNNN CANCNNNGGN 721 NCNTCNNNAN NGGGGNNNNC NGNNNNNNAN NNNNNNNNN //
Primer: EF1a-N_R (Pr0024) Region: EF-1 alpha pro,attB2,attB1,cPPT_CTS Sequence file: RDB12867_B2Abc.seq
>12867_12867_B2Ab_3_EF1a-N_R_C06_03_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNGGNCGA NGTGCGCTCT GCCCACTGAC GGGCACCGGA 61 GCCTCACGAA GCTTGAATTA TCACCACTTT GTACAAGAAA GCTGGGTCTA GATATCTCGA 121 GTGCGGCCGC GAATTCGGTA CCGGATCCAG TCGACTGAAT TGGTTCCTTT AAAGCCTGCT 181 TTTTTGTACA AACTTGTGAT AATTCAAAGT GAATTTCTGC TGTCCCTGTA ATAAACCCGA 241 AAATTTTGAA TTTTTGTAAT TTGTTTTTGT AATTCTTTAG TTTGTATGTC TGTTGCTATT 301 ATGTCTACTA TTCTTTCCCC TGCACTGTAC CCCCCAATCC CCCCTTTTCT TTTAAAATTG 361 TGGATGAATA CTGCCATTTG TAGATCCGTT CACTAATCGA ATGGATCTGT CTCTGTCTCT 421 CTCTCCACCT TCTTCTTCTA TTCCTTCGGG CCTGTCGGGT CCCCTCGGGG TTGGGAGGTG 481 GGTCTGAAAC GATAATGGTG AATATCCCTG CCTAACTCTA TTCACTATAG AAAGTACAGC 541 AAAAACTATT CTTAAACCTA CCAAGCCTCC TACTATCATT ATGAATAATT TTATATACCA 601 CAGCCAATTT GTTATGTTAA ACCAATTCCA CAAACTTGCC CATTTATCTA ATTCCAATAA 661 TTCTTGTTCA TTCTTTTCTT GCTGGTTTTG CGATTCTTCA ATTAAGGAGT GTATTAAGCT 721 TGTGTAATTG TTAATTTCTC TGTCCCACTC CATCCAGGTC GTGTGATTCC NAATCTGTTC 781 CAGAGATTTA TTACTCCAAC TAGCATTCCA AGGCACAGCA GTGGTGCAAA TGAGTTTTCC 841 ANANCANNCC NNAATCCCCA GGANCTGTTG ATNCTTTAGG TATCTTTCNN CAGCCAGGAT 901 TCTNGNCTGN ANCTGCTTGA TGNCCCANAN TGTGAGTTGC AACANATGCT GTTGCGCCTC 961 NNNANCCNNC ANCAAATTGN TCTGNTGCTG CNNNANNNNN GANNATAATT GNCTGGNNNG 1021 NACCNNCAGC GNNATNGANN CTGNNNCCCN NNNNNNNNN //
Primer: BSD_R1 (Pr0758) Region: BSD 5',T2A,Venus 3' Sequence file: RDB12867_B2Abd.seq
>12867_12867_B2Ab_3_BSD_R1_C01_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNTGNAGA NGCGGCCGTC GCTAGAGAGA GCTGCNNCTG 61 GCGACGCTGT AGTCTTCAGA GATGGGGATG CTGTTGATTG TAGCCGTTGC TCTTTCAATG 121 AGGGTGGATT CTTCTTGAGA CAAAGGCTTG GCCATGGGCC CGGGATTTTC CTCCACGTCA 181 CCGCATGTTA GAAGACTTCC TCTGCCCTCG CCGGATCCCT TGTACAGCTC GTCCATGCCG 241 AGAGTGATCC CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG 301 GGGTCTTTGC TCAGGGCGGA CTGGTAGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG 361 CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCC GCCGTCCTCG 421 ATGTTGTGGC GGATCTTGAA GTTGGCCTTG ATGCCGTTCT TCTGCTTGTC GGCGGTGATA 481 TAGACGTTGT GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC 541 TTGAAGTCGA TGCCCTTCNG CTCGATGCGG TTCACCNGGG TGTCGCCCTC NAACTTCNCC 601 TCGGCGCGGG TCTTGNAGTT GCCNTCNNCC TTGAANAANN NGGTGCGCTC CTGGANNTAG 661 CCNTCNGGNN TGGCGGNNTT GNANAANTCN NGNTGNTNNN GNGGNNGGGN ANCNGGCNAN 721 NNNNNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,5' LTR,HIV-1 psi Sequence file: RDB12867_B2Abe.seq
>12867_12867_B2Ab_3_CMV_Forward_A06_01_ABI08.ab1 1 NNNNNNNNNN NNNNCCNTTG NNGCNNNNGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA 61 TAAGCAGCGC GTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG 121 CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT 181 CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT 241 TAGTCAGTGT GGAAAATCTC TAGCAGTGGC GCCCGAACAG GGACTTGAAA GCGAAAGGGA 301 AACCAGAGGA GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG 361 AGGGGCGGCG ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAGGCTAG AAGGAGAGAG 421 ATGGGTGCGA GAGCGTCAGT ATTAAGCGGG GGAGAATTAG ATCGCGATGG GAAAAAATTC 481 GGTTAAGGCC AGGGGGAAAG AAAAAATATA AATTAAAACA TATAGTATGG GCAAGCAGGG 541 AGCTAGAACG ATTCGCAGTT AATCCTGGCC TGTTAGAAAC ATCAGAAGGC TGTAGACAAA 601 TACTGGGACA GCTACAACCA TCCCTTCAGA CAGGATCAGA AGAACTTAGA TCATTATATA 661 ATACAGTAGC AACCCTCTAT TGTGTGCATC AAAGGATAGA GATAAAAGAC ACCAAGGAAG 721 CTTTAGACAA GATAGAGGAA GAGCAAAACA AAAGTAAGAN CACCGCACAG CAAGCGGCCG 781 CTGATCTTCA GACCTGGAGG ANGAGATATG ANGGACAATT GGAGAAGTGA ATTATATAAA 841 TATAAAGTAG TAAAAATTGA NCCNTTANGA GTAGCACCCA CCAAGGCAAA GAGAAGANTG 901 NTGCAGANAG AAAAAAGANC AGNGGGAATA NGANCTTTGT TCCTNGGGNT CTTGGGAGCA 961 NCANNNAGCA CTATGGNCGC AGCGNCNATG NNNNNNNNNG NACNNNCCNN NNNNNTANNG 1021 NCNNGNNNNN NNGNNNNNNN NNN //
Primer: RRE_F1 (Pr0796) Region: RRE,cPPT_CTS Sequence file: RDB12867_B2Abf.seq
>12867_12867_B2Ab_3_RRE_F1_B06_02_ABI08.ab1 1 NNNNNNNNNN NGNNNCNNCN NNNNNNTGCT GANGGCTATT GAGGCGCAAC AGCATCTGTT 61 GCAACTCACA GTCTGGGGCA TCAAGCAGCT CCAGGCAAGA ATCCTGGCTG TGGAAAGATA 121 CCTAAAGGAT CAACAGCTCC TGGGGATTTG GGGTTGCTCT GGAAAACTCA TTTGCACCAC 181 TGCTGTGCCT TGGAATGCTA GTTGGAGTAA TAAATCTCTG GAACAGATTT GGAATCACAC 241 GACCTGGATG GAGTGGGACA GAGAAATTAA CAATTACACA AGCTTAATAC ACTCCTTAAT 301 TGAAGAATCG CAAAACCAGC AAGAAAAGAA TGAACAAGAA TTATTGGAAT TAGATAAATG 361 GGCAAGTTTG TGGAATTGGT TTAACATAAC AAATTGGCTG TGGTATATAA AATTATTCAT 421 AATGATAGTA GGAGGCTTGG TAGGTTTAAG AATAGTTTTT GCTGTACTTT CTATAGTGAA 481 TAGAGTTAGG CAGGGATATT CACCATTATC GTTTCAGACC CACCTCCCAA CCCCGAGGGG 541 ACCCGACAGG CCCGAAGGAA TAGAAGAAGA AGGTGGAGAG AGAGACAGAG ACAGATCCAT 601 TCGATTAGTG AACGGATCTA CAAATGGCAG TATTCATCCA CAATTTTAAA AGAAAAGGGG 661 GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT AATAGCAACA GACATACAAA 721 CTAAAGAATT ACAAAAACAA ATTACAAAAA TTCAAAATTT TCGGGTTTAT TACAGGGACA 781 GCAGAANTTC ACTTTGAATT ATCACAAGTT TGTACNAAAA AGCANGCTTT NAAGGAACCA 841 ATTCAGTCGA CTGGATCCGG TACCGAATTC GCGGCCGCAC TCGANANATC TAGANCCAGC 901 TTTCTTGNAC AAAGTGNTGA NAANTCNNGC TTCGTGAGGN TCCGGNGNCC GTCAGTGGGC 961 ANANCNCACA TCGCCCACAN NNCCCNANAN NNNNGGGGGN ANGGGNCGGN NNTTGAANCN 1021 NNNNNNNNN //
Primer: bGH_rev3 (Pr0885) Region: pGH pA,3' LTR Sequence file: RDB12867_B2Abg.seq
>12867_12867_B2Ab_3_bGH_rev3_G01_19_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN GGNNGGNNNN NNNACAGATG GCTGGCAACT AGAAGGCACA 61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA 121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG 181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC 241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC 301 CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC 361 ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC 421 TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA 481 TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA 541 GATCCNCAGA TCAAGGATAT CTTGTCTTCN TTGGGAGTGA ATTAGCCCTT CCNGTCCCCC 601 CTTTTCTTTT NAAAAGTGGC NNANATCTAC NGCTGNCNTG TAAGTCNTTG GTCTTNAAGG 661 NACNTGAGGN GNGANTGGAA AACCCNNCTC CNNNTCNNTG NGNTCNNNNN NNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,Zeo,SV40 pA Sequence file: RDB12867_B2Abh.seq
>12867_12867_B2Ab_3_SV40pro_ori_F_D06_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNCCTCTG CCTCTGANCT ATTCCNGAAG TAGTGAGGAG 61 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG 121 GATCTGATCA GCACGTGTTG ACAATTAATC ATCGGCATAG TATATCGGCA TAGTATAATA 181 CGACAAGGTG AGGAACTAAA CCATGGCCAA GTTGACCAGT GCCGTTCCGG TGCTCACCGC 241 GCGCGACGTC GCCGGAGCGG TCGAGTTCTG GACCGACCGG CTCGGGTTCT CCCGGGACTT 301 CGTGGAGGAC GACTTCGCCG GTGTGGTCCG GGACGACGTG ACCCTGTTCA TCAGCGCGGT 361 CCAGGACCAG GTGGTGCCGG ACAACACCCT GGCCTGGGTG TGGGTGCGCG GCCTGGACGA 421 GCTGTACGCC GAGTGGTCGG AGGTCGTGTC CACGAACTTC CGGGACGCCT CCGGGCCGGC 481 CATGACCGAG ATCGGCGAGC AGCCGTGGGG GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG 541 CAACTGCGTG CACTTCGTGG CCGAGGAGCA GGACTGACAC GTGCTACGAG ATTTCGATTC 601 CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT 661 GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC 721 AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT 781 TTCACTGCAT TCTAGTTGTG GTTTGTCNNA ACTCATCNAT GTATCTTATC ATGTCTGTAT 841 ACCGTCGACC TCTANCTANA GCTTGNCGTA ATCATGGTCA TANCTGTTTC CTGTGTGAAA 901 NTGNTATCCG CTCACAANTC CNCACNACAT ACGAGCCGNA AGCATNAAGN GTAAAGNCTG 961 GGGTGNCNNN TGAGTGANCT ANCTCACNTT ANTTGCGTTG CNCTNNCNGC CCNCTTTNNN 1021 GNNNGNNNNN NNNCNNNNNN NCNGCNTNNN NNANNNNNNN NNN //