Backbone vector plasmid of lentivirus construct driven by Tet-responsive promoter by Gateway(R) cloning with hKO1-2A-rtTA marker driven by CMV promoter.
Clone info. | Backbone vector plasmid of lentivirus construct driven by Tet-responsive promoter by Gateway(R) cloning with hKO1-2A-rtTA marker driven by CMV promoter. |
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Comment | Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394). |
Vector backbone | CSIV-TRE-RfA-CMV-KT (Plasmid) |
Selectable markers | Ampicillin, Chloramphenicol, ccdB (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. |
Growth remarks | Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine. |
Depositor|Developer | Miyoshi, Hiroyuki | |
Sequence (full) | RDB12876hts01.seq ![]() |
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Remarks, protocol and/or map (pdf) | RDB12876.pdf |
Publication | Isaji, T., J. Biol. Chem. 289 (30): 20694-20705 (2014). [link to RRC of NBRP] |
Test sheet | RDB12876_A9Flp1-2.pdf ![]() |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Ordering instruction.[link] |
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Terms and conditions for distribution | The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Karasawa, S. et al., Biochem. J. 381: 307-312 (2004). |
Remarks | Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。 |
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MTAに書く使用条件 | 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。 付加的使用条件: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Karasawa, S. et al., Biochem. J. 381: 307-312 (2004). |
備考 | 【追加提供依頼書】受入れ確認書(書式J) このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB12876 | CSIV-TRE-RfA-CMV-KT | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The CSIV-TRE-RfA-CMV-KT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12876). |
Reference section:
Isaji, T., Im, S., Gu, W., Wang, Y., Hang, Q., Lu, J., Fukuda, T., Hashii, N., Takakura, D., Kawasaki, N., Miyoshi, H., Gu, J., An oncogenic protein Golgi phosphoprotein 3 up-regulates cell migration via sialylation. J. Biol. Chem. 289 (30): 20694-20705 (2014). PMID 24895123. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Isaji, T., An oncogenic protein Golgi phosphoprotein 3 up-regulates cell migration via sialylation. J. Biol. Chem. 289 (30): 20694-20705 (2014). PMID 24895123. [link to RRC of NBRP] |
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reference | Umei, T.C., Single-Construct Polycistronic Doxycycline-Inducible Vectors Improve Direct Cardiac Reprogramming and Can Be Used to Identify the Critical Timing of Transgene Expression. Int. J. Mol. Sci. 18 (8). pii: E1805 (2017). PMID 28825623. |
reference | Lu, J., Expression of N-Acetylglucosaminyltransferase III Suppresses α2,3-Sialylation, and Its Distinctive Functions in Cell Migration Are Attributed to α2,6-Sialylation Levels. J. Biol. Chem. 291 (11): 5708-5720 (2016). PMID 26801611. |
reference | Tai, M.C., miR-342-3p regulates MYC transcriptional activity via direct repression of E2F1 in human lung cancer. Carcinogenesis 36 (12): 1464-1473 (2015). PMID 26483346. [link to RRC of NBRP] |
reference | Chapnick, D.A., Leader cell positioning drives wound-directed collective migration in TGFβ-stimulated epithelial sheets. Mol. Biol. Cell, 25 (10): 1586-1593 (2014). PMID 24623725. [link to RRC of NBRP] |
reference | Kurita, R., Establishment of immortalized human erythroid progenitor cell lines able to produce enucleated red blood cells. PLoS One 8 (3): e59890 (2013). PMID 23533656. [link to RRC of NBRP] |
user_report | Yamada, N., Inhibition of 7-dehydrocholesterol reductase prevents hepatic ferroptosis under an active state of sterol synthesis. Nat Commun. 2024 Mar 12;15(1):2195 PMID 38472233. [link to RRC of NBRP] |
user_report | Zhang, H., The maturation of iPS cell-derived brain microvascular endothelial cells by inducible-SOX18 expression. Fluids Barriers CNS 20 (1): 10 (2023). PMID 36732767. [link to RRC of NBRP] |
user_report | Zhang, H., Transient ETV2 Expression Promotes the Generation of Mature Endothelial Cells from Human Pluripotent Stem Cells. Biol. Pharm. Bull. 45 (4): 483-490 (2022). PMID 35370273. [link to RRC of NBRP] |
user_report | Orimoto, A., Establishment of human airway epithelial cells with doxycycline-inducible cell growth and fluorescence reporters. Cytotechnology 73 (4): 555-569 (2021). PMID 34349346. [link to RRC of NBRP] |
user_report | Ito, N., Direct reprogramming of fibroblasts into skeletal muscle progenitor cells by transcription factors enriched in undifferentiated subpopulation of satellite cells. Sci. Rep. 7 (1): 8097 (2017). PMID 28808339. [link to RRC of NBRP] |
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