RDB12877 - CSIV-TRE-GFP-UbC-KT

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

CSIV-TRE-GFP-UbC-KT (#RDB12877)

Lentivirus vector plasmid of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by UbC promoter.

Clone info.	Lentivirus vector plasmid of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by UbC promoter.
Comment	Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone	CSIV-TRE-RfA-UbC-KT ()
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor\|Developer	Miyoshi, Hiroyuki \|

Sequence (full)	RDB12877zzk02.seq provided by the depositor
Remarks, protocol and/or map (pdf)	RDB12877.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).

Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。付加的使用条件： When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12877	CSIV-TRE-GFP-UbC-KT	Please contact us.	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSIV-TRE-GFP-UbC-KT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12877).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12877_A5D2p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-N (Pr0027) Region: attB1, tight TRE pro, cPPT_CTS Sequence file: RDB12877_A5D2h.seq
>12877_12877_A5D2_2_EGFP-N_A08_02.ab1 1 NNNNNNNNNN NNNNNCNNNN CNCNNTTGCT NNNNNTGGTG GNGCCTGCTT TTTTGTACNA 61 ACTTGTGATG CTAGCGGCGA TCTGACGGTT CACTAAACGA GCTCTGCTTA TATAGGCCTC 121 CCACCGTACA CGCCTACCTC GACATACGTT CTCTATCACT GATAGGGAGT AAACTCGACA 181 TACGTTCTCT ATCACTGATA GGGATAAACT CGACATACGT TCTCTATCAC TGATAGGGAG 241 TAAACTCGAC ATACGTTCTC TATCACTGAT AGGGAGTAAA CTCGACATAC GTTCTCTATC 301 ACTGATAGGG AGTAAACTCG ACATCGTTCT CTATCACTGA TAGGGAGTAA ACTCGACATA 361 CGTTCTCTAT CACTGATAGG GAGTAAACTC GATTGAAGAC GAAAGGGCCG AATTCAAAGT 421 GAATTTCTGC TGTCCCTGTA ATAAACCCGA AAATTTTGAA TTTTTGTAAT TTGTTTTTGT 481 AATTCTTTAG TTTGTATGTC TGTTGCTATT ATGTCTACTA TTCTTTCCCC TGCACTGTAC 541 CCCCCAATCC CCCCTTTTCT TTTAAAATTG TGGATGAATA CTGCCATTTG TAGATCCGTT 601 CACTAATCGA ATGGATCTGT CTCTGTCTCT CTCTCCACCT TCTTCTTCTA TTCCTTCGGG 661 CCTGTCGGGT CCNNTCGGGG TTGGGAGGTG GGTCTGAAAC GATAATGGTG AATATCCCTG 721 CCTAACTCTA TTCACTATAG AAAGTACAGC AAAAACTATT CTTAAACCTA CCAAGCCTCC 781 TACTATCATT ATGAATAATT TTATATACCA CAGCCNATTT GTTATGTTAA ACCNATTCCA 841 CAAACTTGCC NATTTATCTA ATTNCAATAA TTCTTGTTCA TTCTTTTCTT GCTGGTTTTG 901 CGATTCTNCN ATTAANGANT GNATTAAGCT TGTGTANNTG NTAANTTCTN NGNCCNNCTN 961 CATCCNNGNC NNGNGATTNC NAATCNGNNN CANANNNTNN NNANNNCNNN NNNNNNN //
Primer: mVenus-M_F (Pr0574) Region: EGFP 3', attB2, UbC pro Sequence file: RDB12877_A5D2i.seq
>12887_12887_B1HkF_mVenus-M_F_F01_16_ABI24.ab1 1 NNNNNNNNNN NNNGNNTNNN GGTGNNCTTC NAGATCCGCC ACAACATCGA GGNCGGCAGC 61 GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG 121 CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC 181 GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG 241 CTGTACAAGT CCGGACTCAG ATCCTACTAG TAGGATCTCG AGATATCTAG ACCCAGCTTT 301 CTTGTACAAA GTGGTGATGC TACCGGTCTC GAGATCGGGA GATCTGGCCT CCGCGCCGGG 361 TTTTGGCGCC CCCCGCGGGC GCCCCCCTCC TCACGGCGAG CGCTGCCACG TCAGACGAAG 421 GGCGCAGCGA GCGTCCTGAT CCTTCCGCCC GGACGCTCAG GACAGCGGCC CGCTGCTCAT 481 AAGACTCGGC CTTAGAACCC CAGTATCAGC AGAAGGACAT TTTAGGACGG GACTTGGGTG 541 ACTCTAGGGC ACTGGTTTTC TTTCCAGAGA GCGGAACAGG CGAGGAAAAG TAGTCCCTTC 601 TCGGCGANTC TGCGGANGGA TCTCCGTGGG GCGGTGAACG CCGATGATTA TANNANGACG 661 CGCCNGGGTG TGGCACAGCT AGTTCCGTCN CANNNNGGNN TTGGGTCNNN NNNNNNN //
Primer: UbCpro_F (Pr0158) Region: UbC pro, hKO 5' Sequence file: RDB12877_A5D2j.seq
>12877_12877_A5D2_2_UbCpro_F_C08_08.ab1 1 NNNNNNNNNN NNNGNNTNNG NNNNGTAANT TGTCCGCTNA ATTCTGGCCG TTTTTGGCTT 61 TTTTGTTAGA CCGGACCGTG TTTAGCGCTA CCGGTCCACC ATGGTGAGCG TGATCAAGCC 121 CGAGATGAAG ATGAAGTACT TCATGGACGG CAGCGTGAAC GGCCACGAGT TCACCGTGGA 181 GGGCGAGGGC ACCGGCAAGC CCTACGAGGG CCACCAGGAG ATGACCCTGA GGGTGACAAT 241 GGCCAAGGGC GGCCCCATGC CCTTCAGCTT CGACCTGGTG AGCCACACCT TCTGCTACGG 301 CCACAGGCCC TTCACCAAGT ACCCCGAGGA GATCCCCGAC TACTTCAAGC AGGCCTTCCC 361 CGAGGGCCTG AGCTGGGAGA GGAGCCTCCA GTTCGAGGAC GGCGGCTTCG CCGCCGTGAG 421 CGCCCACATC AGCCTGAGGG GCAACTGCTT CGAGCACAAG AGCAAGTTCG TGGGCGTGAA 481 CTTCCCCGCC GACGGCCCCG TGATGCAGAA CCAGAGCAGC GACTGGGAGC CCAGCACCGA 541 GAAGATCACC ACCTGCGACG GCGTGCTGAA GGGCGACGTG ACCATGTACC TGAAGCTGGC 601 CGGCGGCGGC AACCACAAGT GCCAGTTCAA GACCACCTAC AAGGCCGCCA AGAAGATCCT 661 GAAGATGCCC CAGAGCCACT TCATCGGCCA CAGGCTGGTG ANGAAGANCG AGGGCAACAT 721 CACCGAGCTG GTGGAGGANG CCGTGGCCCA CTGCGAGGGC AGANGAAGTC TTCTAACATG 781 CGGTGACGTG GANGAGAATC CCGGCCNTAT GTCTAGACTG GACNAGANCA AAGTCATAAA 841 CGGCGCTCTG GAATTACTCA ATGGAGTCGG TATCGAANGN CTGACGANNA NGAAACTCGC 901 TCAAAAGCTG GGANTTGANC AGCCNANCNN GNACTNGNNC NNNAANAANN AGCGGGNCNN 961 GNNCGANGCC CNGNCNATCN NNANGNNNGN NNNNNNNNN //
Primer: mKO2-M_F (Pr0578) Region: hKO 5', T2A, rtTA-Advanced Sequence file: RDB12877_A5D2k.seq
>12877_12877_B1HkF_mKO2-M_F_H01_22_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNC NNNNTNNNGN NCNCCTACAA GGCCGCCAAG AAGNTCCTGA 61 AGATGCCCCA GAGCCACTTC ATCGGCCACA GGCTGGTGAG GAAGACCGAG GGCAACATCA 121 CCGAGCTGGT GGAGGACGCC GTGGCCCACT GCGAGGGCAG AGGAAGTCTT CTAACATGCG 181 GTGACGTGGA GGAGAATCCC GGCCCTATGT CTAGACTGGA CAAGAGCAAA GTCATAAACG 241 GCGCTCTGGA ATTACTCAAT GGAGTCGGTA TCGAAGGCCT GACGACAAGG AAACTCGCTC 301 AAAAGCTGGG AGTTGAGCAG CCTACCCTGT ACTGGCACGT GAAGAACAAG CGGGCCCTGC 361 TCGATGCCCT GCCAATCGAG ATGCTGGACA GGCATCATAC CCACTTCTGC CCCCTGGAAG 421 GCGAGTCATG GCAAGACTTT CTGCGGAACA ACGCCAAGTC ATTCCGCTGT GCTCTCCTCT 481 CACATCGCGA CGGGGCTAAA GTGCATCTCG GCACCCGCCC AACAGAGAAA CAGTACGAAA 541 CCCTGGAAAA TCAGCTCGCG TTCCTGTGTC AGCAAGGCTT CTCCCTGGAG AACGCACTGT 601 ACGCTCTGTC CGCCGTGGGC CACTTTACAC TGGGCNGCGT ATTGGANGAA CAGGAGCATC 661 NAGTAGCAAA AGANGNNNAG AGACACCTAC CACCNNTTCT ATGCCCCNNC TTCNGANANN 721 NGCANNTNNN NNGNNNNNNN NGNNNNGGNN NNNNNNANNN NNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro, 5' LTR, HIV-1 psi Sequence file: RDB12877_A5D2l.seq
>12877_12877_A5D2_2_CMV-Forward_E08_14.ab1 1 NNNNNNNNNN NNNNNNNTGN NNNNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT 121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA 181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA 241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA 301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG 361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT 421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG 481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG 541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA 601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT 661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT 721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGCGGCCGCT 781 GATCTTCAGA CCTGGAGGAG GAGATATGAN GGANAATTGG AGAAGTGAAT TATATAAATA 841 TAAAGTAGTA AAAATTGAAC CATTANGAGT AGCACCCNCC NANGCAAAGA GAAGAGNGGT 901 GCAGAGAGAA AAAAGANCAG TGGGAATANG AGCTTNGTTC CTNGGGNNCT NNGGANCAGC 961 NNNAANCACT ATGGNNNCNG CNNNNNTGNN GNNNNNNNNN CNNGNNNNNN NNNN //
Primer: EGFP-M_R (Pr0743) Region: EGFP 5', attB1, tight TRE pro Sequence file: RDB12877_A5D2m.seq
>12877_12877_B1HkF_EGFP-M_R_G01_19_ABI24.ab1 1 NNNNNNNNNN NNNNNNGCGG NNCTNNNNNT NNCCTTGATG CCGTTCTTCT GCTTGTCGGC 61 CATGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC 121 GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA 181 CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG TGCGCTCCTG 241 GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA 301 GCGGCTGAAG CACTGCACGC CGTAGGTCAG GGTGGTCACG AGGGTGGGCC AGGGCACGGG 361 CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTAGGTGG CATCGCCCTC 421 GCCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT CGACCAGGAT 481 GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGGTGGAGC CTGCTTTTTT 541 GTACAAACTT GTGATGCTAG CGGCGATCTG ACGGTTCACT AAACGAGCTC TGCTTATATA 601 GGCCTCCCAC CGTACACGCC TACCTCGACA TACGTTCTCT ATCACTGATA NGGAGTAAAC 661 TCGACATACG TTCTCTATCN CTGANANGGN NAAACTCGAC ATACGTTCTC TATCNNNGNT 721 NNGGNNTAAA NTNNNNNTAC NNTNNTCNNN NNNNNNN //
Primer: PRE_rev (Pr0250) Region: PRE, rtTA-Advanced 3' Sequence file: RDB12877_A5D2n.seq
>12877_12877_B1HkF_PRE_rev_E01_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNCCN NTNAATCTTT NNCAAATTTT GTAATCCAGA GGTTGATTAT 61 CGATAAGCTT GATCCCTCGA TGTTAACTCT AGAGGATCCT TACTTAGTTA CCCGGGGAGC 121 ATGTCAAGGT CAAAATCGTC AAGAGCGTCA GCAGGCAGCA TATCAAGGTC AAAGTCGTCA 181 AGGGCATCGG CTGGGAGCAT GTCTAAGTCA AAATCGTCAA GGGCGTCGGC CGGCCCGCCG 241 CTTTCGCACT TTAGCTGTTT CTCCAGGCCA CATATGATTA GTTCCAGGCC GAAAAGGAAG 301 GCAGGTTCGG CTCCCTGCCG GTCGAACAGC TCAATTGCTT GTCTCAGAAG TGGGGGCATA 361 GAATCGGTGG TAGGTGTCTC TCTTTCCTCT TTTGCTACTT GATGCTCCTG TTCCTCCAAT 421 ACGCAGCCCA GTGTAAAGTG GCCCACGGCG GACAGAGCGT ACAGTGCGTT CTCCNNGGAG 481 AAGCCTTGCT GACNCAGGAA NNNNANCTGA TTTTCCAGGG TTNCNNACTG TTTCTCTGTT 541 GNNNNNNTGN NNNNATGCAC TTTANNCCCG NCNNNANNNG ANANNANAGN ACAGNGNANN 601 GANTTGNNNN NNNNCNNNNN NNNNN //
Primer: bGH_rev3 (Pr0885) Region: bGH pA, 3'LTR, loxP, PRE Sequence file: RDB12877_A5D2o.seq
>12877_12877_B1HkF_bGH_rev3_H03_24_ABI24.ab1 1 NNNNNNNNNN NNNNNGNNNN NCAACTAGAA GGCACAGTCG AGGCTGATCA GCGGGTTTAA 61 ACGGGCCCTG CTAGAGATTT TCCACACTGA CTAAAAGGGT CTGAGGGATC TCTAGTTACC 121 AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT CAAGGCAAGC TTTATTGAGG 181 CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG GCTCAGATCT GGTCTAACCA 241 GAGAGACCCA GTACAGTCCG GAATAACTTC GTATAATGTA TGCTATACGA AGTTATGATA 301 TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC CCTTTTCTTT TAAAAAGTGG 361 CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG GTACCTGAGG TGTGACTGGA 421 AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC AATCAGCATT GGTAGCTGCT 481 GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC GACGGTATCG ATGCGGGGAN 541 GCGGCCCAAA GGGANNTCCG ACTCGTCTGA NGGCGAAGGC GAANNNGCNG AAGANGCCGC 601 ANAGCCGGNN NNNNGCCGCN GGAANGAANG NCCNCTGGNN TGAGGGNCNN ANGGNANGTA 661 NNNNANNNAC GTCCCGCNNN NANTCNNNNN NNNNN //
Primer: SV40pro_F (Pr0110) Region: Zeo, SV40 pA Sequence file: RDB12877_A5D2p.seq
>12877_12877_A5D2_2_SV40pro_F_G08_20.ab1 1 NNNNNNNNNN NNNNNNNNNN NANNNNNGNN GANGNTTTTT TGGAGGNCTA GGCTTTTGCN 61 AAAAGCTCCC GGGAGCTTGT ATATCNNTTT TCGGATCTGA TCAGCACGTG TTGACAATTA 121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC 181 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT 241 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT 301 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC 361 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT 421 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG 481 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA 541 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG 601 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT 661 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA 721 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC 781 CNAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGNC 841 GTAATCATGG TCATAGCTGT TTCCTGTGTG NAATTGTTAT CCGCTCACAA TTCCNCACAA 901 CATACGANCC GGAAGCATAA AGTGTAAAGN CTGGGGTGNC TAATGANTGA GCTAACTCAC 961 ATTAATTGCG NNGCGCTCNC TGNCNNNNTT NCAGNCNGNA AACCNGNCGN GNNNNNNNNN 1021 N //