RDB12882 - CSIV-RfA

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

CSIV-RfA (#RDB12882)

Backbone vector plasmid of lentivirus construct.

Clone info.	Backbone vector plasmid of lentivirus construct. Please note that this construct contains loxP.
Comment	Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone	CSIV-RfA (Plasmid)
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor\|Developer	Miyoshi, Hiroyuki \|

Sequence (full)	RDB12882zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.

Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12882	CSIV-RfA	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSIV-RfA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12882).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12882_B0Jkp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_M_R (Pr0012) Region: CmR, lacUV5 pro, attR1, cPPT_CTS Sequence file: RDB12882_B0Jka.seq
>12882_12882_B0Jk_2_Cm-M_R_F02_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNATNNCNNG CTCNCCGTCT TTNNTTGCCN TACGGAATTC 61 CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT 121 ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT 181 ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC 241 AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT 301 CGACGGATCC TAACTCAAAA TCCACACATT ATACGAGCCG GAAGCATAAA GTGTAAAGCC 361 TGGGGTGCCT AATGCGGCCG CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA 421 GTCTGTTTTT TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT 481 CGTTCAGCTT TTTTGTACAA ACTTGTGATA ATTCAAAGTG AATTTCTGCT GTCCCTGTAA 541 TAAACCCGAA AATTTTGAAT TTTTGTAATT TGTTTTTGTA ATTCTTTAGT TTGTATGTCT 601 GTTGCTATTA TGTCTACTAT TCTTTCCCCT GCACTGTACC CCCCCNATCC CCCCTTTTCN 661 TTNAAAANTG NGGNNNAANA CTGNCATTTG TANANCCNNN CNCTAATCNN ANNNNNNNNG 721 NNNNNGNNNN NNNNNN //
Primer: ccdB-C_F (Pr0136) Region: ccdB, attR2, PRE Sequence file: RDB12882_B0Jkb.seq
>12882_12882_B0Jk_ccdB-C_F_E08_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NGGNNNNNNN NTGGCGCATG ATGACCACCG ATATGGCCAG 61 TGTGCCGGTC TCCGTTATCG GGGAAGAAGT GGCTGATCTC AGCCACCGCG AAAATGACAT 121 CAAAAACGCC ATTAACCTGA TGTTCTGGGG AATATAAATG TCAGGCTCCC TTATACACAG 181 CCAGTCTGCA GGTCGACCAT AGTGACTGGA TATGTTGTGT TTTACAGTAT TATGTAGTCT 241 GTTTTTTATG CAAAATCTAA TTTAATATAT TGATATTTAT ATCATTTTAC GTTTCTCGTT 301 CAGCTTTCTT GTACAAAGTG GTGATGCTAC CGGTCTCGAG CCTCTAGAGT TAACATCGAG 361 GGATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA 421 TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC 481 ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT 541 CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG 601 CTGACNCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCNNCTCCTT TCCGGGACTT 661 TCGCTTTCCC CNTCCCNATT GNNNCNNNNN NNNCATCNNN NNNNGNCNTN NNCCNCTGCT 721 NNNNNNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro, 5' LTR, HIV-1 psi Sequence file: RDB12882_B0Jkc.seq
>12882_12882_B0Jk_CMV-Forward_C08_08_ABI24.ab1 1 NNNNNNNNNN NNNNNTGNNN NNNTGGGCGG TAGGCGTGNN NNGTGGGAGG TCTATATAAG 61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT 121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG 181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT 241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC 301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG 361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG 421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT 481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT 541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT 601 GGGNCAGCTA CAACCATCCC TTCNGACNGG ATCNGAAGAN CTTANATCNT TATATAATAC 661 NGTANCNACC CTCTANNGNG NGNNTCNNNG ATANNNNNAA ANNNNNNNNN N //
Primer: RRE_F1 (Pr0796) Region: RRE, gp41 peptide Sequence file: RDB12882_B0Jkd.seq
>12882_12882_B0Jk_RRE_F1_D08_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN ANNNTTTGCT GANGGCTATT GAGGCGCAAC AGCATCTGTT 61 GCAACTCACA GTCTGGGGCA TCAAGCAGCT CCAGGCAAGA ATCCTGGCTG TGGAAAGATA 121 CCTAAAGGAT CAACAGCTCC TGGGGATTTG GGGTTGCTCT GGAAAACTCA TTTGCACCAC 181 TGCTGTGCCT TGGAATGCTA GTTGGAGTAA TAAATCTCTG GAACAGATTT GGAATCACAC 241 GACCTGGATG GAGTGGGACA GAGAAATTAA CAATTACACA AGCTTAATAC ACTCCTTAAT 301 TGAAGAATCG CAAAACCAGC AAGAAAAGAA TGAACAAGAA TTATTGGAAT TAGATAAATG 361 GGCAAGTTTG TGGAATTGGT TTAACATAAC AAATTGGCTG TGGTATATAA AATTATTCAT 421 AATGATAGTA GGAGGCTTGG TAGGTTTAAG AATAGTTTTT GCTGTACTTT CTATAGTGAA 481 TAGAGTTAGG CAGGGATATT CACCATTATC GTTTCAGACC CACCTCCCAA CCCCGAGGGG 541 ACCCGACAGG CCCGAANGAA TAGAAGAAGA ANGTGGAGAG AGAGACAGAN ACAGATCCNT 601 TCGATTAGTG AACGGNTCTA CNAATGGCAG TATTCNTCCN CAATNNNAAA AGAAAAGGGG 661 GGNNTGGGGG GGNACAGNGN NNNNNNGNAN AGTANANNTN NNNNCNNNNG NNNNNNNANN 721 NNNNNNNTNN NNAANNNANN NNNNNNNN //
Primer: bGH_rev3 (Pr0885) Region: bGHpA, 3' LTR, loxP, PRE Sequence file: RDB12882_B0Jke.seq
>12882_12882_B0Jk_bGH_rev3_F08_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN GNNGGGCAAA CAACAGATGG CTGGCAACTA GAAGGCACAG 61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTGCTAGAGA TTTTCCACAC TGACTAAAAG 121 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC 181 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC 241 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGT CCGGAATAAC TTCGTATAAT 301 GTATGCTATA CGAAGTTATG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC 361 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA 421 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG 481 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT 541 CTCGACGGTA TCGATGCGGG GAGGCGGCCC AAAGGGAGAT CCGACTCGTC TGANGGCGAA 601 NGCGAAGANG CGGAANANGN CGCAGANCCG GCAGCANGNC GCGGGAANGA ANGNNCGCTG 661 GNNTGAGGGN CNAANNGNAC GNANNNNNNN NNGTCCNNCG NNNANTNNNN NNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, EM7 pro, Zeo Sequence file: RDB12882_B0Jkf.seq
>12882_12882_B0Jk_SV40pro_ori_F_G08_20_ABI24.ab1 1 NNNNNNNNNN NNGNCGCCTC TGCCTCTGAN CTATTCCNGA AGTAGTGAGG AGGCTTTTTT 61 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 121 CAGCACGTGT TGACAATTAA TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG 181 TGAGGAACTA AACCATGGCC AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG 241 TCGCCGGAGC GGTCGAGTTC TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG 301 ACGACTTCGC CGGTGTGGTC CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC 361 AGGTGGTGCC GGACAACACC CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG 421 CCGAGTGGTC GGAGGTCGTG TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG 481 AGATCGGCGA GCAGCCGTGG GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG 541 TGCACTTCGT GGCCGANGAN CANGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG 601 CCTTCTATGA AAGNNTGGGC TTCNGANTCG TTTTCCGGGA NGCCNGCTGG ATGANNCTCC 661 NGCGCGGGGN NCTCNTGCTG NANTNCNTCG CCNNCCCNNN NNNNNTNNNN NNNNNN //