RDB12884 - CSIV-RfA-CG

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

CSIV-RfA-CG (#RDB12884)

Comment	Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone	Plasmid
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor\|Developer	Miyoshi, Hiroyuki \|

Sequence (full)	RDB12884zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.

Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12884	CSIV-RfA-CG	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSIV-RfA-CG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12884).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12884_B3Gep1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_M_R (Pr0012) Region: CmR,attR1,luc UV50 pro,cPPT_CTS Sequence file: RDB12884_B3Gea.seq
>12884_12884_B3Ge_1_Cm_M_R_F02_06_ABI08.ab1 1 NNNNNNNNNN NNNNNCNNNT ATNNCNNGCT CNCCNNCTTN NNNTGCCATA CGGAATTCCG 61 GATGNAGCAT TCATCAGGCG GGCAAGAATG TGAATAAAGG CCGGATAAAA CTTGTGCTTA 121 TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA ATATCCAGCT GAACGGTCTG GTTATAGGTA 181 CATTGAGCAA CTGACTGAAA TGCCTCAAAA TGTTCTTTAC GATGCCATTG GGATATATCA 241 ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC 301 GACGGATCCT AACTCAAAAT CCACACATTA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 361 GGGGTGCCTA ATGCGGCCGC CATAGTGACT GGATATGTTG TGTTTTACAG TATTATGTAG 421 TCTGTTTTTT ATGCAAAATC TAATTTAATA TATTGATATT TATATCATTT TACGTTTCTC 481 GTTCAGCTTT TTTGTACAAA CTTGTGATAA TTCAAAGTGA ATTTCTGCTG TCCCTGTAAT 541 AAACCCGAAA ATTTTGAATT TTTGTAATTT GTTTTTGTAA TTCTTTAGTT TGTATGTCTG 601 TTGCTATTAT GTCTACTATT CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT 661 TAAAATTGTG GNNNNANACT GNCNTTTGTA GATNCGTTNA CNNANTCNNN NNNNNNTGTC 721 NCTNNCTCTC TCNNNNNNNT NNNNNNNNNN //
Primer: ccdb-M_F (Pr0951) Region: ccdB,attR2,CMV en.2 Sequence file: RDB12884_B3Geb.seq
>12884_12884_B3Ge_1_ccdb-M_F_E01_05_ABI08.ab1 1 NNNNNNNNNN NNNNGNNNNA NTGNNTGNNC TNNGCNNCCG CGNAAATGAC ATCAAAAACG 61 CCATTAACCT GATGTTCTGG GGAATATAAA TGTCAGGCTC CCTTATACAC AGCCAGTCTG 121 CAGGTCGACC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT CTGTTTTTTA 181 TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG TTCAGCTTTC 241 TTGTACAAAG TGGTGATAAT TCGATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 301 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 361 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 421 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 481 TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 541 CTGGCATTAT GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT ACATCTACGT 601 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA 661 GCGGTTTGAC TCACGGGGNN TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 721 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC CATTGACGCA 781 AATGNNCGGT ANGCGTGTAC GGNGGGNNGN CTATATAAGC AGANCTNGTT TANTGAANCG 841 TCANANCCGC TANCGCTACN NNNCGCCACN NNGGNGANCA ANGGCNANNN NCNNNTCNNN 901 NGGGNNNNNN NCNNTCCNNN NNNANNNNGN NNNGNNNNNN NNANNNNNNN NNN //
Primer: PRE_rev (Pr0250) Region: PRE,EGFP 3' Sequence file: RDB12884_B3Gec.seq
>12884_12884_B3Ge_1_PRE_rev_D02_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNCAACATA GTTAAGAATA CCAGTCAATC TTTCACAAAT 61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGAGGCT 121 CGAGATCCTA CTAGTAGGAT CTGAGTCCGG ACTTGTACAG CTCGTCCATG CCGAGAGTGA 181 TCCCGGCGGC GGTCACGAAC TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT 241 TGCTCAGGGC GGACTGGGTG CTCAGGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC 301 CGATGGGGGT GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC GCTGCCGTCC TCGATGTTGT 361 GGCGGATCTT GAAGTTCACC TTGATGCCGT TCTTCTGCTT GTCGGCCATG ATATAGACGT 421 TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT 481 CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC 541 GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGANGGTGCG CTCCTGGACG TAGCCTTCGG 601 GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG CTGAAGCACT 661 GCACGCCGNN NNTCNNNNNG GTCACNANGG TGGGNCNGGG NNCNGNNNGN NTNNNNNNNN 721 GNNNNNNNNN N //
Primer: HIV-B_R (Pr0673) Region: 5' LTR,CMV pro.1,CMV en.1 Sequence file: RDB12884_B3Ged.seq
>12884_12884_B3Ge_1_HIV-B_R_C02_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNAA NNTTTTGNNG TANTCNCCAG TCGCCGCCCC TCGCCTCTTG 61 CCGTGCNNNN NNNNNNNNGC CGAGTCCTGC GTCGAGAGAG CTCCTCTGGN TNNNNTTTCG 121 CTTTCAAGTC CCTGTTCGGG CGCCACTGCT AGAGATTTTC CACACTGACT AAAAGGGTCT 181 GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA 241 AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC 301 TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGGCAAAA CGCGCTGCTT ATATAGACCT 361 CCCACCGTAC ACGCCTACCG CCCATTTGCG TCAATGGGGC GGAGTTGTTA CGACATTTTG 421 GAAAGTCCCG TTGATTTTGG TGCCAAAACA AACTCCCATT GACGTCAATG GGGTGGAGAC 481 TTGGAAATCC CCGTGAGTCA AACCGCTATC CACGCCCATT GATGTACTGC CAAAACCGCA 541 TCACCATGGT AATAGCGATG ACTAATACGT AGATGTACTG CCAAGTAGGA AAGTCCCATA 601 AGGTCATGTA CTGGGCATAA TGCCAGGCGG GCCATTTACC GTCATTGACG TCAATAGGGG 661 GCGTACTTGG CATATGATAC ACTTGATGTA CTGCCNAGTG GGCAGTTTAC CGTAAATACT 721 CCACCCATTG ACGTCAATGG AAAGTCCCTA TTGNCGTTAC TATGGGAACA TACGTCATTA 781 TTGACGTCNA TGNNNGGGGG TCGTTGGGCG GNCAGCCAGG CGGGCCATTT ANCGTAAGTT 841 ATGTAACGCN GNANNTNNNT ATATNGNCTA TNANCTAATG ANCCCNNNAT TGANTNCTAT 901 TNANNACTAN NCNATNANCN ANGNNNNNNN NNATATCTGG NCNGTNNNTC NNNNANNNNN 961 NNNAANNNNN NNANNNNNNN NNN //
Primer: EGFP-N_R2 (Pr0905) Region: EGFP 5',CMV pro.2,CMV en.2 Sequence file: RDB12884_B3Gee.seq
>12884_12884_B3Ge_1_EGFP-N_R2_G02_07_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNGCNNNN CNNNNCNNNC CGNANGTCAG GGNGGTCACG 61 AGGGTGGGCC AGGGNACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG 121 CCGTAGGTGG CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG 181 CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC 241 ATGGTGGCGA CCGGTAGCGC TAGCGGATCT GACGGTTCAC TAAACCAGCT CTGCTTATAT 301 AGACCTCCCA CCGTACACGC CTACCGCCCA TTTGCGTCAA TGGGGCGGAG TTGTTACGAC 361 ATTTTGGAAA GTCCCGTTGA TTTTGGTGCC AAAACAAACT CCCATTGACG TCAATGGGGT 421 GGAGACTTGG AAATCCCCGT GAGTCAAACC GCTATCCACG CCCATTGATG TACTGCCAAA 481 ACCGCATCAC CATGGTAATA GCGATGACTA ATACGTAGAT GTACTGCCAA GTAGGAAAGT 541 CCCATAAGGT CATGTACTGG GCATAATGCC AGGCGGGCCA TTTACCGTCA TTGACGTCAA 601 TAGGGGGCGT ACTTGGCATA TGATACACTT GNTGTACTGC CNAGTGGGCA GTTTACCGTA 661 AATACTCNAC CCATTGACGT CNATGNAAAG TCCCNTATTN NNNNTACTAN NGGNNNCNTA 721 CNNNNNTNNN TNNNNNNNNN N //
Primer: EGFP-M_R (Pr0743) Region: EGFP 5',CMV pro.2 Sequence file: RDB12884_B3Gef.seq
>12884_12884_B3Ge_1_EGFP-M_R_H02_08_ABI08.ab1 1 NNNNNNNNNN NNNNGNGGNG GNNCTTGNNN TTCNCCTTGA TGCCGTTCTT CTGCTTGTCG 61 GCCATGATAT AGACGTTGTG GCTGTTGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG 121 CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG 181 AACTTCACCT CGGCGCGGGT CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC 241 TGGACGTAGC CTTCGGGCAT GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG 301 TAGCGGCTGA AGCACTGCAC GCCGTAGGTC AGGGTGGTCA CGAGGGTGGG CCAGGGCACG 361 GGCAGCTTGC CGGTGGTGCA GATGAACTTC AGGGTCAGCT TGCCGTAGGT GGCATCGCCC 421 TCGCCCTCGC CGGACACGCT GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG 481 ATGGGCACCA CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA CCATGGTGGC GACCGGTAGC 541 GCTAGCGGAT CTGACGGTTC ACTAAACCAG CTCTGCTTAT ATAGACCTCC CACCGTACAC 601 GCCTACCGCC CATTTGCGTC AATGGGGCGG ANTTGTTACG ACATTTTGGA AAGTNCCGTT 661 GNNTTTGGTG NCNAAACAAA CTCCCATTGN CGTCNANNGG GGNNNNNNNN NN //
Primer: bGH_rev3 (Pr0885) Region: 3' LTR,loxP,PRE Sequence file: RDB12884_B3Geg.seq
>12884_12884_B3Ge_1_bGH_rev3_F01_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NGGGCNNNNA ACAGNNGGCT GGCAACTAGA AGGCACAGTC 61 GAGGCTGATC NNNNNNTTTA AACGGGCCCT GCTAGAGATT TTCCACACTG ACTAAAAGGG 121 TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC 181 TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA 241 GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGAATAACTT CGTATAATGT 301 ATGCTATACG AAGTTATGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC 361 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA 421 GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA 481 CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCATGTTT TTCTAGGTCT 541 CGACGGTATC GATGCGGGGA GGCGGCCCAA AGGGAGATCC GACTCGTCTG AGGGCGAAGG 601 CGAAGACGCG GAAGAGGCCG CAGAGCCGGC AGCAGGCCGC GGGAAGGAAG GTCCGCTGGA 661 TTGAGGGCCG AANGGACGTA NCAGAANGAC GTCCCGCGCA GAATCCAGGT GGCAACACAG 721 GCGANCAGCC ATGGAAAGGA CGTCAGCTTC CCCGACAACA CCACGGAATT GTCAGTGCCC 781 AACAGCCGAN CCCCTGTCCA GCANCGGGCA AGGCANGCGG CGATGANTTC CGCNNNNNCN 841 ATANGGANGG GGGAAAGCGA ANNTCCNNGA AANGANCTGA NANGNNGNGG CAANGNCCNN 901 NCCAGNGGGG GGTTGCGNCN NCNAACACNG NGCNNNNNAN NNCNNNNNTN NNCNNNNNNN 961 NNGGNNNNNN NNNN //
Primer: SV40pro_ori_F (Pr0731) Region: Zeo,SV40 pA Sequence file: RDB12884_B3Geh.seq
>12884_12884_B3Ge_1_SV40pro_ori_F_G02_20_ABI24.ab1 1 NNNNNNNNNN NNNNNGCCNC CTCTGCCTCT GANCTATTCN NGAAGTAGTG AGGAGGCTTT 61 TTTGGAGNNN NNNNNNNTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT NNNNNNATCT 121 GATCAGCACG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA 181 AGGTGAGGAA CTAAACCATG GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG 241 ACGTCGCCGG AGCGGTCGAG TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG 301 AGGACGACTT CGCCGGTGTG GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG 361 ACCAGGTGGT GCCGGACAAC ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT 421 ACGCCGAGTG GTCGGAGGTC GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA 481 CCGAGATCGG CGAGCAGCCG TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT 541 GCGTGCACTT CGTGGCCGAG GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG 601 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 661 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 721 ATAATGNTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 781 TGCATTCTAN TTGTGGTTTG TCCNAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 841 CNANCTCTAN CTAGANCTTG GCGTAATCAT GGNCATANCN GNTNCCTGNG NGAAATTGTT 901 NTCCNCTCNC NATTCCNCNN NNNATACNAN CCNGANNNAT AAANNGNAAA GNCNNGGGNN 961 GNCNNNNNNN NNN //