RDB12885 - CS-RfA-UbCG

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

CS-RfA-UbCG (#RDB12885)

Comment	Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone	Plasmid
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth remarks	Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor\|Developer	Miyoshi, Hiroyuki \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.

Remarks Remember that you will be working with samples containing infectious virus.
Use Survival2 (Invitrogen) or equivalent to amplify this clone.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12885	CS-RfA-UbCG	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CS-RfA-UbCG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12885).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12885_B2Eup1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_N_rev (Pr0014) Region: CmR,attR1,cPPT_CTS Sequence file: RDB12885_B2Eua.seq
>12885_12885_B2Fu_1_Cm_N_rev_A04_01_ABI24.ab1 1 NNNNNNNNNN NNNTCTTNNN GATGCNNTTG GGNNNTATCA ACGGTGGTAT ATCCAGTGAT 61 TTTTTTCTCC NTTTTAGCTT CCTTAGCTCC TGAAAATCTC GACGGATCCT AACTCAAAAT 121 CCACACATTA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGCGGCCGC 181 CATAGTGACT GGATATGTTG TGTTTTACAG TATTATGTAG TCTGTTTTTT ATGCAAAATC 241 TAATTTAATA TATTGATATT TATATCATTT TACGTTTCTC GTTCAGCTTT TTTGTACAAA 301 CTTGTGATAA TTCAAAGTGA ATTTCTGCTG TCCCTGTAAT AAACCCGAAA ATTTTGAATT 361 TTTGTAATTT GTTTTTGTAA TTCTTTAGTT TGTATGTCTG TTGCTATTAT GTCTACTATT 421 CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT TAAAATTGTG GATGAATACT 481 GCCATTTGTA GATCCGTTCA CTAATCGAAT GGATCTGTCT CTGTCTCTCT CTCCACCTTC 541 TTCTTCTATT CCTTCGGGCC TGTCGGGTCC CCTCGGGGTT GGGAGGTGGG TCTGAAACGA 601 TAATGGTGAA TATCCCTGCC TAACTCTATT CACTATAGAA AGTACAGCAA AAACTATTCT 661 TAAACCTACC AAGCCTCCTA CTATCATTAT GAATAATTTT ATATACCACA GCCAATTTGT 721 TATGTTAAAC CAATTCCACA AACTTGCCCA TTTATCTAAT TCCAATAATT CTTGTTCATT 781 CTTTTCTTGC TGGTTTTGCG ATTCTTCAAT TAANGAGTGN ATTAAGCTTG TGTAATTGTT 841 AATTTCTCTG TCCCACTCCA TCCAGGTCGT GTGATTCCNA ATCTGTTCCN GANNNTTATT 901 ACTCCNACTA NCATTCCNNN GNACAGCANN GGTGCAAATG ANTTTNNCNN ANCNNNCNNN 961 AATCCCNANN ANCTGNNNNT NCTTTNNNNA TCNTTNNNNA NCCNNGNANN CNTNNNNNNN 1021 NNCNNNNNNN NNN //
Primer: ccdB-C_F (Pr0136) Region: ccdB,attR2,UbC pro Sequence file: RDB12885_B2Eub.seq
>12885_12885_B2Fu_1_ccdB-C_F_B04_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNTC NNGGNNNAAG CTGGCGCATG NNTGNCCNCC GATATGGCCN 61 GTGTGCCGGT CTCCGTTATC GGGGAAGAAG TGGCTGATCT CAGCCACCGC GAAAATGACA 121 TCAAAAACGC CATTAACCTG ATGTTCTGGG GAATATAAAT GTCAGGCTCC CTTATACACA 181 GCCAGTCTGC AGGTCGACCA TAGTGACTGG ATATGTTGTG TTTTACAGTA TTATGTAGTC 241 TGTTTTTTAT GCAAAATCTA ATTTAATATA TTGATATTTA TATCATTTTA CGTTTCTCGT 301 TCAGCTTTCT TGTACAAAGT GGTGATAATT CTAGCTCGAG ATCGGGAGAT CTGGCCTCCG 361 CGCCGGGTTT TGGCGCCCCC CGCGGGCGCC CCCCTCCTCA CGGCGAGCGC TGCCACGTCA 421 GACGAAGGGC GCAGCGAGCG TCCTGATCCT TCCGCCCGGA CGCTCAGGAC AGCGGCCCGC 481 TGCTCATAAG ACTCGGCCTT AGAACCCCAG TATCAGCAGA AGGACATTTT AGGACGGGAC 541 TTGGGTGACT CTAGGGCACT GGTTTTCTTT CCAGAGAGCG GAACAGGCGA GGAAAAGTAG 601 TCCCTTCTCG GCGATTCTGC GGANGGATCT CCGTGGGGCG GTGAACGCCG ATGATTATAT 661 AAGGACGCGC CGGGTGTGGC ACAGCTAGTT CCGTCGCAGC CGGGATTTGG GTCGCGGTTC 721 TTGTTTGTGG ATCGCTGTGA TCGTCACTTG NTGAGTAGCG GGCTGCTGGG CTGGNCGNNN 781 CTTTCGTGGC CGCCGGGCCG CTCGGTGGGA NGGAANCGTG TGNAGAGACC GCCNAGGGCT 841 GTAGTCTGGG NNCNCGAGCA AGGTTGNCCT GAACTGGGGG TNGGGGGGGA NCGCANCNAA 901 ATGGCGGCNG TTNNNNNNNC TTGNNNNNNA ANNNGCTTGN GANGNGNNNN NNGANGNCNN 961 NNAAANNNNN NNNGGGGGGN NANNGNNNNN NNGNNNGNAN NNNNNNNNN //
Primer: UbCpro_F (Pr0158) Region: UbC pro,EGFP 5' Sequence file: RDB12885_B2Euc.seq
>12885_12885_B2Fu_1_UbCpro_F_C04_07_ABI24.ab1 1 NNNNNNNNNN NNNNNNGNGT NNNNNNNGTA AATTGTCCGC TAAATTCTGG CCGTTTTTGG 61 CTTTTTTGTT AGACCGGACC GTGTTTAGCG CTACCGGTCG CCACCATGGT GAGCAAGGGC 121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC 181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG 241 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG 301 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC 361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC 421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG 481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC 541 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC 601 TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG 661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GANCACCCAG 721 TCCGCCCTGA NCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGANTTCGTG 781 ANCGCCGCCG GGATCACTCT CGGCATGGAC GANCTGTACA AGTCCGGANT CAGATCCTAC 841 TANTANGANC TCGAGGNNTC AAGCTTATCG ATAATCAACC TCNGGANTAN AAAATTTGTG 901 AAAGANTGNN NGGNATTCTT AACTNTGTTG NNCCTTTTAC GCTATGNGGA TNNNCNGCTT 961 TNNNGNCNTT GTATCNNGCN NNNNNNNCCN NNANNNNNTN NNTTTNNNNN NNNNN //
Primer: PRE_rev (Pr0250) Region: PRE,EGFP 3' Sequence file: RDB12885_B2Eud.seq
>12885_12885_B2Fu_1_PRE_rev_D04_10_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNAGCNNCAT AGTTAAGANT ACCAGTCAAT CTTTCNCAAA 61 TTTTGTAATC CNNANGTTGA TTATCGATAA GCTTGATCCC TCGAGATCCT ACTAGTAGGA 121 TCTGAGTCCG GACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA 181 CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG CGGACTGGGT 241 GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG 301 GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTCAC 361 CTTGATGCCG TTCTTCTGCT TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA 421 CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT 481 GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC 541 GTCCTTGAAG AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA 601 GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GCTGAAGCAC TGCACGCCGT ANGTCAGGGT 661 GGTCACGAGG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT 721 CAGCTTGCCG TANGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT 781 TACGTCGCCG TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCNT 841 GCTCACCNTG GTGGCGACCG GNANCGCTNA ACACGGNNCN GTCTAACAAA AAAGCCNAAA 901 ACGGNCAGAA TTTANCGNAN NATTTACTAN TCTAACNNTG AAAATTANNT NNNGNCCNNA 961 ANGANTACNT TTCNAANNNN NNCNNNAAAA ANNNNNNNNA ANNNNNNNNN N //
Primer: CMV_Forward (Pr0016) Region: CMV pro,5' LTR,HIV-1 psi Sequence file: RDB12885_B2Eue.seq
>12885_12885_B2Fu_1_CMV_Forward_E04_13_ABI24.ab1 1 NNNNNNNNNN NNNNNNNTGN NGCNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT 121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA 181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA 241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA 301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG 361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT 421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG 481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG 541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA 601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT 661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT 721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGCGGCCGCT 781 GATCTTCAGA NCTGGANGAN GANATATGAG GGACAATTGG AGAAGTGAAT TATATAAATA 841 TAAAGTAGTA AAAATTGAAC CATTANGAGN NNNNNCCACC NAGGNAAAGA NAANANTGGN 901 GCANANANAA AAAAGANCAG TGGGNATAGG ANCTTTGNTC CNNGGNNTCT NGGGNNCAGC 961 NNNAANCNCT NTGGNNNNNN CNNNNNNNNN N //
Primer: RRE-M_R (Pr0651) Region: PPE,HIV-1 psi Sequence file: RDB12885_B2Euf.seq
>12885_12885_B2Fu_1_PRE_rev_D04_10_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNAGCNNCAT AGTTAAGANT ACCAGTCAAT CTTTCNCAAA 61 TTTTGTAATC CNNANGTTGA TTATCGATAA GCTTGATCCC TCGAGATCCT ACTAGTAGGA 121 TCTGAGTCCG GACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA 181 CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG CGGACTGGGT 241 GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG 301 GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTCAC 361 CTTGATGCCG TTCTTCTGCT TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA 421 CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT 481 GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC 541 GTCCTTGAAG AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA 601 GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GCTGAAGCAC TGCACGCCGT ANGTCAGGGT 661 GGTCACGAGG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT 721 CAGCTTGCCG TANGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT 781 TACGTCGCCG TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCNT 841 GCTCACCNTG GTGGCGACCG GNANCGCTNA ACACGGNNCN GTCTAACAAA AAAGCCNAAA 901 ACGGNCAGAA TTTANCGNAN NATTTACTAN TCTAACNNTG AAAATTANNT NNNGNCCNNA 961 ANGANTACNT TTCNAANNNN NNCNNNAAAA ANNNNNNNNA ANNNNNNNNN N //
Primer: bGH_rev3 (Pr0885) Region: 3' LTR,PRE Sequence file: RDB12885_B2Eug.seq
>12885_12885_B2Fu_1_bGH_rev3_G04_19_ABI24.ab1 1 NNNNNNNNNN NNNNNGGNNG NNGGNNNNNN NNAGANGGCT GGCAACTAGA AGGCACAGTC 61 GAGGCTGNTC NNCGGGTTTA AACGGGCCCT GCTAGAGATT TTCCACACTG ACTAAAAGGG 121 TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC 181 TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA 241 GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGATGCAGCT CTCGGGCCAT 301 GTGATGAAAT GCTAGGCGGC TGTCAAACCT CCACTCTAAT ACTTCTCTCT CCGGGTCATC 361 CATCCCATGC AGGCTCACAG GGTGTAACAA GCGGGTGTTC TCTCCTTCAT TGGCTTCTTC 421 TACCTTCTCT TGCTCAACTG GTACTAGCTT GTAGCACCAT CCAAAGGTCA GTGGATATCT 481 GATCCCTGGC CCTGGTGTGT AGTTCTGCCA ATCAGGGAAG TAGCCTTGTG TGTGGTAGAT 541 CCACAGATCA AGGATATCTT GTCTTCGTTG GGAGTGAATT AGCCCTTCCA GTCCCCCCTT 601 TTCTTTTAAA AAGTGGCTAA GATCTACAGC TGCCTTGTAA GTCATTGGTC TTAAAGGTAC 661 CTGAGGTGTG ACTGGAAAAC CCACCTCCTC CTCCTCTTGT GCTTCTAGCC AGGCACAATC 721 AGCATTGGTA GCTGCTGTAT TGCTACTTGT GATTGCTCCA TGTTTTTCTA GGTCTCGACG 781 GTATCGATGC GGGGNNGCGG CCCNAAGGGA GATCCGANTC GTCTGANGGC GANNGCGAAG 841 ANGCGGAANA NGCCGCANAN CCGGCAGCAN GCCGCGGGAA NGAANGNCCG CTGGNNTGAN 901 GGNCGAANNG NANGTANCAN ANNNNNGTCC CGCGNANAAT CCNNNTGGNN NCNCNNNCGA 961 NCANNNNTGN NAAGGNNNNC NNCNNNCCNN NNNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,Zeo Sequence file: RDB12885_B2Euh.seq
>12885_12885_B2Fu_1_SV40pro_ori_F_H04_22_ABI24.ab1 1 NNNNNNNNNN NNNNNNNCGC CTCTGCCTCT GANCTATTCN NGAAGTAGTG AGGAGGCTTT 61 TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT 121 GATCAGCACG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA 181 AGGTGAGGAA CTAAACCATG GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG 241 ACGTCGCCGG AGCGGTCGAG TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG 301 AGGACGACTT CGCCGGTGTG GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG 361 ACCAGGTGGT GCCGGACAAC ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT 421 ACGCCGAGTG GTCGGAGGTC GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA 481 CCGAGATCGG CGAGCAGCCG TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT 541 GCGTGCACTT CGTGGCCGAG GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG 601 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC 661 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT 721 ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC 781 TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC TGTATACCGT 841 CNACCTCTAG CTAGAGCTTG NNGTAATCAT GGTCATAGCT GNTTCCTGTG TGAAATTGNT 901 ATNCGCTCNC NNTTCCNCAC NACATACNAN CCGGNANNAT AAANNGNAAA NCCNGGGGNN 961 NCTNNNGANT GANCTNNCTN NNNTTAATTG NNTTGNNCTN NNNNNCNNNN TNNNNNNNNN 1021 N //