RDB12908 - CSII-EF-hOct3/4-IRES2-Venus

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icon below as well as clone information before placing your order.

CSII-EF-hOct3/4-IRES2-Venus (#RDB12908)

Lentivirus vector plasmid of human OCT3/4 for generating iPS cells.

Clone info.	Lentivirus vector plasmid of human OCT3/4 for generating iPS cells.
Comment	Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone	Plasmid
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name	Human POU5F1 cDNA
Depositor\|Developer	Miyoshi, Hiroyuki \|

Sequence	RDB12908z.seq
Remarks, protocol and/or map (pdf)	RDB12908.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents. Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。付加的使用条件： When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12908	CSII-EF-hOct3/4-IRES2-Venus	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII-EF-hOct3/4-IRES2-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12908).

Reference section:

Fujioka, T., Shimizu, N., Yoshino, K., Miyoshi, H., Nakamura, Y., Establishment of induced pluripotent stem cells from human neonatal tissues. Hum. Cell 23 (3): 113-118 (2010). PMID 20973836. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12908_A4Jfp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F2 Sequence file: RDB12908_A4Jfa.seq
>12908_12908_A4Jf_pAxEF_F2_1_D02_11.ab1 1 ATCTTCATTT AGTGTCGTGA CACGCTACCG GTCTCGAGAA TTCTGCAGCG GCCATCACAA 61 GTTTGTACAA AAAAGCAGGC TCCGCGGCCG CCCCCTTCAC CATGGCGGGA CACCTGGCTT 121 CGGATTTCGC CTTCTCGCCC CCTCCAGGTG GTGGAGGTGA TGGGCCAGGG GGGCCGGAGC 181 CGGGCTGGGT TGATCCTCGG ACCTGGCTAA GCTTCCAAGG CCCTCCTGGA GGGCCAGGAA 241 TCGGGCCGGG GGTTGGGCCA GGCTCTGAGG TGTGGGGGAT TCCCCCATGC CCCCCGCCGT 301 ATGAGTTCTG TGGGGGGATG GCGTACTGTG GGCCCCAGGT TGGAGTGGGG CTAGTGCCCC 361 AAGGCGGCTT GGAGACCTCT CAGCCTGAGG GCGAAGCAGG AGTCGGGGTG GAGAGCAACT 421 CCGATGGGGC CTCCCCGGAG CCCTGCACCG TCACCCCTGG TGCCGTGAAG CTGGAGAAGG 481 AGAAGCTGGA GCAAAACCCG GAGGAGTCCC AGGACATCAA AGCTCTGCAG AAAGAACTCG 541 AGCAATTTGC CAAGCTCCTG AAGCAGAAGA GGATCACCCT GGGATATACA CAGGCCGATG 601 TGGGGCTCAC CCTGGGGGTT CTATTTGGGA AGGTATTCAG CCAAACGACC ATCTGCCGCT 661 TTGAGGCTCT GCAGCTTAGC TTCAAGAACA TGTGTAAGCT GCGGCCCTTG CTGCAGAAGT 721 GGGTGGAGGA AGCTGACAAC AATGAAAATC TTCAGGAGAT ATGCAAAGCA GAAACCCTCG 781 TGCAGGCCCG AAAGAGAAAG CGAACCAGTA TCGAGAACCG AGTGAGAGGC AACCTGGAGA 841 ATTTGTTCCT GCAGTGCCCG AAACCCACAC TGCAGCAGAT CAGCCACATC GCCCAGCAGC 901 TTGGGCTCGA GAAGGATGTG GTCCGAGTGT GGGTTCTGTA ACCGGCGCCA GAAGGCAAGC 961 GATCAAGCAG CGACTATGCA CAACGAGAGG ATTTTGAGGC TGCTGGGTCT CCTTTTCTCA 1021 GGGGGACAGT GTCCTTTTCT CTGGGCCCCA GGACCCATTT TGCTACCCCA GCCTATGGCA 1081 GTCCGTTCAC TGCACTGGCA CTGTACTCCC TTCGGTTCCC A //
Primer: IRES-R Sequence file: RDB12908_A4Jfb.seq
>12908_12908_A4Jf_IRES_R_1_H02_23.ab1 1 GCAAAACCGG GCTTCGGCAG TAACGTTAGG GGGGGGGGAG GGAGAGGGGC GGATCCGCGG 61 CCATCACCAC TTTGTACAAG AAAGCTGGGT CGGCGCGCCC ACCCTTACCT CAGTTTGAAT 121 GCATGGGAGA GCCCAGAGTG GTGACGGAGA CAGGGGGAAA GGCTTCCCCC TCAGGGAAAG 181 GGACCGAGGA GTACAGTGCA GTGAAGTGAG GGCTCCCATA GCCTGGGGTA CCAAAATGGG 241 GCCCTGGGGC CAGAGGAAAG GACACTGGTC CCCCTGAGAA AGGAGACCCA GCAGCCTCAA 301 AATCCTCTCG TTGTGCATAG TCGCTGCTTG ATCGCTTGCC CTTCTGGCGC CGGTTACAGA 361 ACCACACTCG GACCACATCC TTCTCGAGCC CAAGCTGCTG GGCGATGTGG CTGATCTGCT 421 GCAGTGTGGG TTTCGGGCAC TGCAGGAACA AATTCTCCAG GTTGCCTCTC ACTCGGTTCT 481 CGATACTGGT TCGCTTTCTC TTTCGGGCCT GCACGAGGGT TTCTGCTTTG CATATCTCCT 541 GAAGATTTTC ATTGTTGTCA GCTTCCTCCA CCCACTTCTG CAGCAAGGGC CGCAGCTTAC 601 ACATGTTCTT GAAGCTAAGC TGCAGAGCCT CAAAGCGGCA GATGGTCGTT TGGCTGAATA 661 CCTTCCCAAA TAGAACCCCC AGGGTGAGCC CCACATCGGC CTGTGTATAT CCCAGGGTGA 721 TCCTCTTCTG CTTCAGGAGC TTGGCAAATT GCTCGAGTTC TTTCTGCAGA GCTTTGATGT 781 CCTGGGACTC CTCCGGGTTT TGCTCCAGCT TCTCCTTCTC CAGCTTCACG GCACCAGGGG 841 TGACGGTGCA GGGCTCCGGG GAGGCCCCAT CGGAGTTGCT CTCCACCCCG ACTCCTGCTT 901 CGCCCTCAGG CTGAGAGGTC TCCAAGCCGC CTTGGGGCAC TAGCCCCACT CCAACCTGGG 961 GCCCACAGTA CGCCATCCCC CCACAGAACT CATACGGCGG GGGGGCATGG GGGGAATCCC 1021 CACACTCAGA GCCTGACCAA CCCCCGGGCC GATTCCTGGT CCTCCAGGAG GGCTTGAGCT 1081 AAGCAGGATC CGAGGATCAC CAAGCCGCTT CCGACTCCTT GACCATTCAC TTCAACCATC 1141 CTTGG //
Primer: IRES-F Sequence file: RDB12908_A4Jfc.seq
>12908_12908_A4Jf-IRES_F_1_E02_14.ab1 1 GCCTTCCTTT TCATGTGTTT AGTTCGAGGT TAAAAAACGT CTAGGCCCCC CGAACCACGG 61 GGACGTGGTT TTCCTTTGAA AAACACGATG ATAATATGGC CACAACCATG GTGAGCAAGG 121 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG 181 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC 241 TGAAGCTGAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC 301 TGGGCTACGG CCTGCAGTGC TTCGCCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT 361 TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG 421 GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG 481 AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA 541 ACTACAACAG CCACAACGTC TATATCACCG CCGACAAGCA GAAGAACGGC ATCAAGGCCA 601 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCGGCGTGCA GCTCGCCGAC CACTACCAGC 661 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCTACC 721 AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG 781 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAGAA TTCAAGGCCT 841 CTCGAGCCTC TAGAGTTAAA CATCGAGGGA TCAAGCTTAT CGATAATCAA CCTCTGGATT 901 ACAAAATTTG TGAAAGATTG ACTGGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT 961 GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC 1021 TCCTCCTTGT ATAATCCTGG GTTGCTGTCT CTTATGAGAG GTTGTGGCCC GTTGTCAGCC 1081 ACGTGGCCGT ATGTGCACTG TGTTTGGCTG ACGCACCCCC CCACTGTTGG GGCCATTGGC 1141 CAACCCCA //
Primer: CMV-Forward Sequence file: RDB12908_A4Jfd.seq
>12908_12908_A4Jf_CMV_Forward_1_C02_08.ab1 1 CGTTAAACTC GGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT 121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA 181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA 241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA 301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG 361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT 421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG 481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG 541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA 601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT 661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT 721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGGGCCGCTG 781 ATCTTCAGAC CTGGAGGAGG AGATATGAGG GACAATTGGA GAAGTGAATT ATATAAATAT 841 AAAGTAGTAA AAATTGAACC ATTAGGAGTA GCACCCACCA AGGCAAAGAG AAGAGTGGTG 901 CAGAGAGAAA AAAGAGCAGT GGGAATAGGA GCTTTGTTCC TTGGGTTCTT GGGAGCAGCA 961 GGAAGCACTA TGGGCGCAGC GTCAATGACG CTGACGGTAC AGGCCAGACA ATTATTTGTC 1021 TGGGTATAGT GCAGCAGCCA GAACAATTTG CTGAAGGGGC TATTGAGCGC AACCAGCCAT 1081 CTGGTTGGCA CTCACAGTCT GGGGGCATCC AAGCAGCTCC AAGGGCCAAA GGA //
Primer: BGH_rev Sequence file: RDB12908_A4Jfe.seq
>12908_12908_A4Jf_BGH_rev_1_F02_17.ab1 1 GCTAGACGGG ACTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT 61 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT 121 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA 181 ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC CATGTGATGA AATGCTAGGC 241 GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC ATCCATCCCA TGCAGGCTCA 301 CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC TTCTACCTTC TCTTGCTCAA 361 CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA TCTGATCCCT GGCCCTGGTG 421 TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA GATCCACAGA TCAAGGATAT 481 CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC CTTTTCTTTT AAAAAGTGGC 541 TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG TACCTGAGGT GTGACTGGAA 601 AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA ATCAGCATTG GTAGCTGCTG 661 TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG ACGGTATCGA TGCGGGGAGG 721 CGGCCCAAAG GGAGATCCGA CTCGTCTGAG GGCGAAGGCG AAGACGCGGA AGAGGCCGCA 781 GAGCCGGCAG CAGGCCGCGG GAAGGAAGGT CCGCTGGATT GAGGGCCGAA GGGACGTAGC 841 AGAAGGACGT CCCGCGCAGA ATCCAGGTGG CAACACAGGC GAGCAGCCAT GGAAAGGACG 901 TCAGCTTCCC CGACAACACC ACGGAATTGT CAGTGCCCAA CAGCCGAGCC CCTGTCCAGC 961 AGCGGGCAAA GGCAGGCGGC GATGAGTTCC GCCGTGGCAA TAGGGAGGGG GAAAGCGAAA 1021 GTCCCGGGAA GGAGCTGACA GGTGGTGGCA ATGCCCCAAC CAGTGGGGGG TTGCGTCAGC 1081 AAACACAGTG CACACACGTC ACGATTGCTG ACACGGACAC ACTTCTTCAT AAGAGACAGC 1141 CAACCAAGGG ATTTTATTAC A //
Primer: SV40pro_F Sequence file: RDB12908_A4Jff.seq
>12908_12908_A4JF_SV40pro_F_1_G02_20.ab1 1 GCTTGGTCCT GGACATCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA 61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA 121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC 181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC 241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC 301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC 361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG 421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG 481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG 541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC 601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG 661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT 721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC 781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG 841 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC 901 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGGTGCC TAATGAGTGA GCTAACTCAC 961 ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA 1021 TTAATGAAAT CGGCCCAACG GCGCGGGGGA GAGGCGGTTT GCGTATTGGG CGGCTCTTCG 1081 CCTTCTCGCT CACTGAACTC GCTGCGCTCG GATCGATCGG CTGCGGCGAG TCGGATCAGC 1141 TCCATCATAG GGCTCGGTTT A //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Fujioka, T., Establishment of induced pluripotent stem cells from human neonatal tissues. Hum. Cell 23 (3): 113-118 (2010). PMID 20973836. [link to RRC of NBRP]
reference	Honda, A., Naive-like conversion overcomes the limited differentiation capacity of induced pluripotent stem cells. J. Biol. Chem. 288 (36): 26157-26166 (2013). PMID 23880763. [link to Journal]
user_report	Hayashi, R., Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium. PLoS One 7 (9): e45435 (2012). PMID 23029008. [link to RRC of NBRP]