RDB12909 - CSII-EF-hSox2-IRES2-Venus

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

CSII-EF-hSox2-IRES2-Venus (#RDB12909)

Lentivirus vector plasmid of human SOX2 for generating iPS cells.

Clone info.	Lentivirus vector plasmid of human SOX2 for generating iPS cells.
Comment	Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone	Plasmid
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name	Human SOX2 cDNA
Depositor\|Developer	Miyoshi, Hiroyuki \|

Sequence	RDB12909z.seq
Remarks, protocol and/or map (pdf)	RDB12909.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).

Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents. Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。付加的使用条件： When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Nagai, T. et al., Nat. Biotechnol. 20: 87-90 (2002).
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12909	CSII-EF-hSox2-IRES2-Venus	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CSII-EF-hSox2-IRES2-Venus was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12909).

Reference section:

Fujioka, T., Shimizu, N., Yoshino, K., Miyoshi, H., Nakamura, Y., Establishment of induced pluripotent stem cells from human neonatal tissues. Hum. Cell 23 (3): 113-118 (2010). PMID 20973836. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12909_A4Jfp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F2 Sequence file: RDB12909_A4Jfa.seq
>12909_12909_A4Jf_pAxEF_F2_2_B03_06.ab1 1 AAATCCCCAT TTAGGTGTCG GTGACACGCT ACCGGTCTCG AGAATTCTGC AGCGGCCATC 61 ACAAGTTTGT ACAAAAAAGC AGGCTCCGCG GCCGCCCCCT TCACCATGTA CAACATGATG 121 GAGACGGAGC TGAAGCCGCC GGGCCCGCAG CAAACTTCGG GGGGCGGCGG CGGCAACTCC 181 ACCGCGGCGG CGGCCGGCGG CAACCAGAAA AACAGCCCGG ACCGCGTCAA GCGGCCCATG 241 AATGCCTTCA TGGTGTGGTC CCGCGGGCAG CGGCGCAAGA TGGCCCAGGA GAACCCCAAG 301 ATGCACAACT CGGAGATCAG CAAGCGCCTG GGCGCCGAGT GGAAACTTTT GTCGGAGACG 361 GAGAAGCGGC CGTTCATCGA CGAGGCTAAG CGGCTGCGAG CGCTGCACAT GAAGGAGCAC 421 CCGGATTATA AATACCGGCC CCGGCGGAAA ACCAAGACGC TCATGAAGAA GGATAAGTAC 481 ACGCTGCCCG GCGGGCTGCT GGCCCCCGGC GGCAATAGCA TGGCGAGCGG GGTCGGGGTG 541 GGCGCCGGCC TGGGCGCGGG CGTGAACCAG CGCATGGACA GTTACGCGCA CATGAACGGC 601 TGGAGCAACG GCAGCTACAG CATGATGCAG GACCAGCTGG GCTACCCGCA GCACCCGGGC 661 CTCAATGCGC ACGGCGCAGC GCAGATGCAG CCCATGCACC GCTACGACGT GAGCGCCCTG 721 CAGTACAACT CCATGACCAG CTCGCAGACC TACATGAACG GCTCGCCCAC CTACAGCATG 781 TCCTACTCGC AGCAGGGCAC CCCTGGCATG GCTCTTGGCT CCATGGGTTC GGTGGTCAAG 841 TCCGAGGCCA GCTCCAGCCC CCCTGTGGTT ACCTCTTCCT CCCACTCCAG GGCGCCCTGC 901 CAGGCCGGGG ACCTCCGGGA CATGATCAGC ATGTATCTCC CCGGCGCCGA GGTGCCGGAA 961 CCCGCCGCCC CCAGCAGACT TCACATGTCC CAGCACTACC AGAGCGGCCC GGTGCCCGGC 1021 ACGGCCATTA ACGGCACACT GCCCCTCTCA CACATGTGAA AGGGTGGGGC GCGCCGACCC 1081 AGCTTTCTGT ACAAGTTGGT TGATTGCGCG GATCGTCCTC TCCTCCTTCT AACGATACTG 1141 ATCGGAAGA //
Primer: IRES-R Sequence file: RDB12909_A4Jfb.seq
>12909_12909_A4Jf_IRES_R_2_F03_18.ab1 1 GCATCAGCGG CCTTCGGCCA GTAACGTTAG GGGGGGGGGA GGGAGAGGGG CGGATCCGCG 61 GCCATCACCA CTTTGTACAA GAAAGCTGGG TCGGCGCGCC CACCCTTTCA CATGTGTGAG 121 AGGGGCAGTG TGCCGTTAAT GGCCGTGCCG GGCACCGGGC CGCTCTGGTA GTGCTGGGAC 181 ATGTGAAGTC TGCTGGGGGC GGCGGGTTCC GGCACCTCGG CGCCGGGGAG ATACATGCTG 241 ATCATGTCCC GGAGGTCCCC GGCCTGGCAG GGCGCCCTGG AGTGGGAGGA AGAGGTAACC 301 ACAGGGGGGC TGGAGCTGGC CTCGGACTTG ACCACCGAAC CCATGGAGCC AAGAGCCATG 361 CCAGGGGTGC CCTGCTGCGA GTAGGACATG CTGTAGGTGG GCGAGCCGTT CATGTAGGTC 421 TGCGAGCTGG TCATGGAGTT GTACTGCAGG GCGCTCACGT CGTAGCGGTG CATGGGCTGC 481 ATCTGCGCTG CGCCGTGCGC ATTGAGGCCC GGGTGCTGCG GGTAGCCCAG CTGGTCCTGC 541 ATCATGCTGT AGCTGCCGTT GCTCCAGCCG TTCATGTGCG CGTAACTGTC CATGCGCTGG 601 TTCACGCCCG CGCCCAGGCC GGCGCCCACC CCGACCCCGC TCGCCATGCT ATTGCCGCCG 661 GGGGCCAGCA GCCCGCCGGG CAGCGTGTAC TTATCCTTCT TCATGAGCGT CTTGGTTTTC 721 CGCCGGGGCC GGTATTTATA ATCCGGGTGC TCCTTCATGT GCAGCGCTCG CAGCCGCTTA 781 GCCTCGTCGA TGAACGGCCG CTTCTCCGTC TCCGACAAAA GTTTCCACTC GGCGCCCAGG 841 CGCTTGCTGA TCTCCGAGTT GTGCATCTTG GGGTTCTCCT GGGCCATCTT GCGCCGCTGC 901 CCGCGGGACC ACACCATGAA GGCATTCATG GGCCGCTTGA CGCGGTCCGG GCTGTTTTTC 961 TGGTTTGCCG CCGGCCGCCG CCGCGGTGGA GTTGCCCGCC GCCGCCCCCC CGAAGTTTGC 1021 TGCGGGCCCG GGCGGCTTCA GCTTCCGTCT TCCATCATGT GTTACATGAT TGAGGGGGCG 1081 TGCGGAGCCT GCTTTTTGGT ACCAACTGTG AATGATGGCT GGCAGAAATT TCTTCGAGGA 1141 ACTCG //
Primer: IRES-F Sequence file: RDB12909_A4Jfc.seq
>12909_12909_A4Jf_IRES_F_2_C03_09.ab1 1 CATTCCCCCT TTCATGTGTT TAGTCGAGGT TAAAAAACGT CTAGGCCCCC CGAACCACGG 61 GGACGTGGTT TTCCTTTGAA AAACACGATG ATAATATGGC CACAACCATG GTGAGCAAGG 121 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG 181 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC 241 TGAAGCTGAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC 301 TGGGCTACGG CCTGCAGTGC TTCGCCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT 361 TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG 421 GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG 481 AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA 541 ACTACAACAG CCACAACGTC TATATCACCG CCGACAAGCA GAAGAACGGC ATCAAGGCCA 601 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCGGCGTGCA GCTCGCCGAC CACTACCAGC 661 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCTACC 721 AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG 781 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAGAA TTCAAGGCCT 841 CTCGAGCCTC TAGAGTTAAC ATCGAGGGAT CAAGCTTATC GATAATCAAC CTCTGGATTA 901 CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG 961 ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC 1021 CTCCTTGTAT AAATCCTGGT TTGCTGTCTC TTTATGAGGA GTTTGTGGGC CCGTGTCAGG 1081 CAACGGTGAC GTGGTGTGCA CTTGTGTTGG CCTTGACGCA AACCCCCACT TGGTTGGGGG 1141 CCAATTGGCC AACCAACT //
Primer: CMV-Forward Sequence file: RDB12909_A4Jfd.seq
>12909_12909_A4Jf_CMV_Forward_2_A03_03.ab1 1 CTTAACCTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGCGCGTT TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC 121 TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA 181 GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG 241 TCAGTGTGGA AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC 301 CAGAGGAGCT CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG 361 GGCGGCGACT GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG 421 GGTGCGAGAG CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT 481 TAAGGCCAGG GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC 541 TAGAACGATT CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAAGGCTGT AGACAAATAC 601 TGGGACAGCT ACAACCATCC CTTCAGACAG GATCAGAAGA ACTTAGATCA TTATATAATA 661 CAGTAGCAAC CCTCTATTGT GTGCATCAAA GGATAGAGAT AAAAGACACC AAGGAAGCTT 721 TAGACAAGAT AGAGGAAGAG CAAAACAAAA GTAAGACCAC CGCACAGCAA GGGCCGCTGA 781 TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA 841 AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGGCAAAGAG AAGAGTGGTG 901 CAGAGAGAAA AAAGAGCAGT GGGGAAATAG GAGCTTTGTT CCCTTGGGTT CTTGGGGAGC 961 AGCAGGAAGC ACTATGGGGC GCAGCGGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA 1021 TTGTCTGGTA TAGTGCAGCC AGCAGACATT TGCTGAAGGC TATTGGAGGG CGCAACAGCC 1081 ATCTGATGCA ACTTCACAGT CTGGGGGCCA TTCAAGTCAA GCTCCAGGCC AAGGAT //
Primer: BGH_rev Sequence file: RDB12909_A4Jfe.seq
>12909_12909_A4Jf_BGH_rev_2_D03_12.ab1 1 GTATATCCGG GGCCCTGCTA GAGATTTTCC ACACTGACTA AAAGGGTCTG AGGGATCTCT 61 AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG AAGCACTCAA GGCAAGCTTT 121 ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG CTCCCAGGCT CAGATCTGGT 181 CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG GGCCATGTGA TGAAATGCTA 241 GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG GTCATCCATC CCATGCAGGC 301 TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC TTCTTCTACC TTCTCTTGCT 361 CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG ATATCTGATC CCTGGCCCTG 421 GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG GTAGATCCAC AGATCAAGGA 481 TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC CCCCTTTTCT TTTAAAAAGT 541 GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA AGGTACCTGA GGTGTGACTG 601 GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC ACAATCAGCA TTGGTAGCTG 661 CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC TCGACGGTAT CGATGCGGGG 721 AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG GCGAAGACGC GGAAGAGGCC 781 GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG ATTGAGGGCC GAAGGGACGT 841 AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA GGCGAGCAGC CATGGAAAGG 901 ACGTCAGCTT CCCCGACAAC ACCACGGAAT TGTCAGTGCC CAACAGCCGA GCCCCTGTCC 961 AGCAGCGGGC AAGGCAAGGC GGCGATGAGT TCCCGCCGTG GCAATAGGGA GGGGGAAAAG 1021 CGAAAGTCCC GGGAAAGGAG CTGACAGGTG GTGCAATGCC CCAACCAGTT GGGGGGTTGC 1081 CGTCAGCCAA ACACAGTGCA ACACAACGTC AACGTTGTCT GACCACCGGG CCACAACTTC 1141 TCATTAAGAG GACAGGCCAA CCCAAG //
Primer: SV40pro_F Sequence file: RDB12909_A4Jff.seq
>12909_12909_A4Jf_SV40pro_F_2_E03_15.ab1 1 CTTTCTTTCC GGGGACAATC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GTTGACAATT 121 AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA GGTGAGGAAC TAAACCATGG 181 CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA CGTCGCCGGA GCGGTCGAGT 241 TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA GGACGACTTC GCCGGTGTGG 301 TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA CCAGGTGGTG CCGGACAACA 361 CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA CGCCGAGTGG TCGGAGGTCG 421 TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC CGAGATCGGC GAGCAGCCGT 481 GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG CGTGCACTTC GTGGCCGAGG 541 AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG 601 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC 661 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA 721 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT 781 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG 841 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA 901 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA 961 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC 1021 ATTAATGAAT CGGGCCAACG CGCGGGGGAG AGGCGGGTTT GCGTATTGGG CGCCTCTTCC 1081 GCTTCCTCGC TCACTGGACC TCGCCTGCCG CTCGGTTCGA TCCGGACTTG CGGCCGAGCC 1141 GTATCAGCTC ACTTTCAAAG GTCGGTTTA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Fujioka, T., Establishment of induced pluripotent stem cells from human neonatal tissues. Hum. Cell 23 (3): 113-118 (2010). PMID 20973836. [link to RRC of NBRP]
user_report	Hayashi, R., Generation of corneal epithelial cells from induced pluripotent stem cells derived from human dermal fibroblast and corneal limbal epithelium. PLoS One 7 (9): e45435 (2012). PMID 23029008. [link to RRC of NBRP]