RDB12946 - CS-hp53-shRNA-CG

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

CS-hp53-shRNA-CG (#RDB12946)

Lentivirus vector plasmid of shRNA targeting human TP53 with GFP marker.

Clone info.	Lentivirus vector plasmid of shRNA targeting human TP53 with GFP marker.
Comment	Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone	Plasmid
Selectable markers	Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Gene/insert name	Human TP53 cDNA
Depositor\|Developer	Miyoshi, Hiroyuki \|

Sequence	RDB12946z.seq
Remarks, protocol and/or map (pdf)	RDB12946.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Ordering instruction.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.

Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
Please contact us. We will provide you with appropriate documents.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Ordering instruction.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
MTAに書く使用条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12946	CS-hp53-shRNA-CG	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Ordering instruction of plasmids [in Japanese] [in English]
Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CS-hp53-shRNA-CG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12946).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12946_A4K5p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: cPPT+CTS_v2_F Sequence file: RDB12946_A4K5a.seq
>12946_12946_A4K5_2_cPPT+CTS_v2_F_B09_06.ab1 1 TCCTAGGAAA TCGGACTAAA GAATTACAAA AACAAATTAC AAAAAATTCA AAATTTTCGG 61 GTTTATTACA GGGACAGCAG AAATTCACTT TGAATTATCA CAAGTTTGTA CAAAAAAGCA 121 GGCTCCACCA TGGGAACCAA TTCAGTCGAC TGGATCCGGT ACCGAATTCG AACGCTGACG 181 TCATCAACCC GCTCCAAGGA ATCGCGGGCC CAGTGTCACT AGGCGGGAAC ACCCAGCGCG 241 CGTGCGCCCT GGCAGGAAGA TGGCTGTGAG GGACAGGGGA GTGGCGCCCT GCAATATTTG 301 CATGTCGCTA TGTGTTCTGG GAAATCACCA TAAACGTGAA ATGTCTTTGG ATTTGGGAAT 361 CTTATAAGTC CCTATCAGTG ATAGAGATCC CCACTCTAGT GGTGATTTAC TACGTGTGCT 421 GTCCGTAGTA GATTACCACT GGAGTTTTTT GGAAATCTAG ACCCAGCTTT CTTGTACAAA 481 GTGGTGATAA TTCGATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT 541 GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC 601 CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA 661 TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA 721 TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA 781 TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT 841 CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACATCAAT GGGCGTGGAT AGCGGTTTGA 901 CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT GGGAGTTTGT TTTGGCACCA 961 AAATCAACGG GACTTTCCAA AAATGTCGTA ACAACTCCGC CCCAATTGAC GCAAAATGGG 1021 CGGTAGGCGT GTACGGGTGG GGAGGTCTAA TATAAAGCAG AGCTGGGTTT TAGTGAAACG 1081 TTCAGATCCG CTAGCGCTTA CGATTCGCAT CATGATTGAG CCAAGGACGG AGGAAGCTGT 1141 TTCAACCCGG GGGGTTGACG //
Primer: PRE_rev Sequence file: RDB12946_A4K5b.seq
>12946_12946_A4K5_2_PRE_rev_C09_09.ab1 1 CGGGATTCAA ATAGCGTAAA GGAGCACATA GTTAAGAATA CCAGTCAATC TTTCACAAAT 61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGAGATCCTA CTAGTAGGAT 121 CTGAGTCCGG ACTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC 181 TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGGGC GGACTGGGTG 241 CTCAGGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG 301 TAGTGGTCGG CGAGCTGCAC GCTGCCGTCC TCGATGTTGT GGCGGATCTT GAAGTTCACC 361 TTGATGCCGT TCTTCTGCTT GTCGGCCATG ATATAGACGT TGTGGCTGTT GTAGTTGTAC 421 TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG 481 CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG 541 TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG 601 TCGTGCTGCT TCATGTGGTC GGGGTAGCGG CTGAAGCACT GCACGCCGTA GGTCAGGGTG 661 GTCACGAGGG TGGGCCAGGG CACGGGCAGC TTGCCGGTGG TGCAGATGAA CTTCAGGGTC 721 AGCTTGCCGT AGGTGGCATC GCCCTCGCCC TCGCCGGACA CGCTGAACTT GTGGCCGTTT 781 ACGTCGCCGT CCAGCTCGAC CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG 841 CTCACCATGG TGGCGACCGG TAGCGCTAGC GGATCTGACG GTTCACTAAA CCAGCTCTGC 901 TTATATAGAC CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGGAGTTG 961 TTACGACATT TTGGGAAAGT CCCGTTTGAT TTTGGGTGCC CAAAACAAAC TCCCCATTGA 1021 CGTCATGGGG TGAGACTGTA ATCCCCCGTG AGTCAACCGC TATCCACGCC CAATGAATGT 1081 ACTGCCTAAG CGCATCACAT GATAATAGCG AATGAACTAA TTACGTAGAA ATGGTTAC //
Primer: HIVgp_R1 Sequence file: RDB12946_A4K5c.seq
>12946_12946_A4K5_HIVgp_R1_2_A01_01.ab1 1 CCGGCCTCCT ATTCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG 61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA 121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGCTTGCT GTGCGGTGGT 181 CTTACTTTTG TTTTGCTCTT CCTCTATCTT GTCTAAAGCT TCCTTGGTGT CTTTTATCTC 241 TATCCTTTGA TGCACACAAT AGAGGGTTGC TACTGTATTA TATAATGATC TAAGTTCTTC 301 TGATCCTGTC TGAAGGGATG GTTGTAGCTG TCCCAGTATT TGTCTACAGC CTTCTGATGT 361 TTCTAACAGG CCAGGATTAA CTGCGAATCG TTCTAGCTCC CTGCTTGCCC ATACTATATG 421 TTTTAATTTA TATTTTTTCT TTCCCCCTGG CCTTAACCGA ATTTTTTCCC ATCGCGATCT 481 AATTCTCCCC CGCTTAATAC TGACGCTCTC GCACCCATCT CTCTCCTTCT AGCCTCCGCT 541 AGTCAAAATT TTTGGCGTAC TCACCAGTCG CCGCCCCTCG CCTCTTGCCG TGCGCGCTTC 601 AGCAAGCCGA GTCCTGCGTC GAGAGAGCTC CTCTGGTTTC CCTTTCGCTT TCAAGTCCCT 661 GTTCGGGCGC CACTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG 721 TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG CAAGCTTTAT 781 TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT 841 AACCAGAGAG ACCCAGTACA GGCAAAACGC GCTGCTTATA TAGACCTCCC ACCGTACACG 901 CCTACCGCCC ATTTGCGTCA ATGGGGGCGG AGTTGTTACG ACATTTTGGA AAGTCCCGTT 961 GATTTTGGGT GCCAAAACAA ACTCCCATTG ACGTCAATGG GGGTGGAGAC TTGGGAAATC 1021 CCCGTGAGTC AACCCGCTAT CCACGCCCAT TGATGTACTG CCAAAACCGC ATCACCCATG 1081 GTAATAGCCG GATGACTATA CGGTAGATGT ACTGCCAAGG TAGAAAGTCC CATAAGTCAT 1141 GTACTTGGGC ATAATGCCAG GGCGGGGCCC AATTTTAACC CGGTTCCA //
Primer: BGH_rev2 Sequence file: RDB12946_A4K5d.seq
>12946_12946_A4K5_2_BGHrev2_D09_12.ab1 1 CATTTGGTTC AAGAGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC 61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA 121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA 181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT 241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG 301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT 361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT 421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT 481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT 541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC 601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG 661 GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC 721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC 781 GACGGTATCG ATGCGGGGAG GCGGCCCAAA GGGAGATCCG ACTCGTCTGA GGGCGAAGGC 841 GAAGACGCGG AAGAGGCCGC AGAGCCGGCA GCAGGCCGCG GGAAGGAAAG GTCCGCTGGA 901 TTGAGGGCCG AAAGGGACGT AGCAGAACGA CGTCCCGCGC AGAATCCAGG TGGCAACACA 961 GGCGAGCAGC CATGGGAAAG GGACGTCAGC TTCCCCGACA CACCCACGGG AATGTCAGTG 1021 CCCACAGCCG AGCCCCTGTC AGCAGCGGGC AAGCAGGCGC CGATGAGTCG GCGTGTCATT 1081 AGAGGGGGAA AGCGAAGTTC CTGCAAGGAG CTGAACAAGG TTGGGGTGGG CCA //
Primer: SV40pro_F Sequence file: RDB12946_A4K5e.seq
>RDB12946_12946_A4K5_SV40pro_F_2_H12_24.ab1 1 CCCTGTCCTT GCTTTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC 121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA 181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG 241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG 301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT 361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC 421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG 481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA 541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT 601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA 661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG 721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA 781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA 841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT 901 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT 961 AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA 1021 ATGAATCGGC CAACGCGCGG GGAGAGGCGG GTTTGCGTAT TGGCGCTCTT CCGCTTTCCT 1081 CGCTCACTGA CTCGCTGCGC TCGGGTCGTT TCGGCTGCGG CGAGCGTATC AGCTCACTCA 1141 AAAGCGTATA CGGCTATCCA CAGGAATCAG GATTACGCGA AGCAAAGGAA ATCCAGTAGT 1201 CCGTGGC //