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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pHTB Cas9 (#RDB12994)

In vitro production of Cas9 mRNA.

Clone info. In vitro production of Cas9 mRNA.
Comment Linearize this plasmid with NotI and synthesize mRNA by utilizing T3 promoter.
Vector backbone pBS-HTB/N (Plasmid)
Size of vector backbone 3.2 kb
Selectable markers Amp^r
Gene/insert name Streptococcus pyogenes Cas9 genomic DNA
Depositor|Developer Sasakura, Yasunori |
 
Sequence (full) RDB12994zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Dev Growth Differ., 56, 499-510(2014)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Dev Growth Differ., 56, 499-510(2014))。 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
備考
本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB12994 pHTB Cas9 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pHTB Cas9 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12994).

Reference section:

Sasaki, H., Yoshida, K., Hozumi, A., Sasakura, Y., CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis. Dev. Growth Differ. 56 (7): 499-510 (2014). PMID 25212715. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12994_A4Jnp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: reverse-2 (Pr0100)
Region: insert 5'
Sequence file: RDB12994_A4Jna.seq check
>RDB12994_3_reverse2_A06_03.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCNNNN NNNNNNCTCN CTAAAGGGAA CNAAAGCTGG
   61 AGCTCCNCAA TTCGGCAGAG TTAAGACCAG ATTCTTAGTT TTAGTTGAAG TGACTGGACG
  121 CGTACTGTCT GTGTCCGGCT TTTTTCTTTA TCTCACAGCC AAATCACTAA ATATCACCGG
  181 ATCCGATAAT GGATAAGAAA TACTCAATAG GCTTAGATAT CGGCACAAAT AGCGTCGGAT
  241 GGGCGGTGAT CACTGATGAA TATAAGGTTC CGTCTAAAAA GTTCAAGGTT CTGGGAAATA
  301 CAGACCGCCA CAGTATCAAA AAAAATCTTA TAGGGGCTCT TTTATTTGAC AGTGGAGAGA
  361 CAGCGGAAGC GACTCGTCTC AAACGGACAG CTCGTAGAAG GTATACACGT CGGAAGAATC
  421 GTATTTGTTA TCTACAGGAG ATTTTTTCAA ATGAGATGGC GAAAGTAGAT GATAGTTTCT
  481 TTCATCGACT TGAAGAGTCT TTTTTGGTGG AAGAAGACAA GAAGCATGAA CGTCATCCTA
  541 TTTTTGGAAA TATAGTAGAT GAAGTTGCTT ATCATGAGAA ATATCCAACT ATCTATCATC
  601 TGCGAAAAAA ATTGGTAGAT TCTACTGATA AAGCGGATTT GCGCTTAATC TATTTGGCCT
  661 TAGCGCATAT GATTAAGTTT CGTGGTCATT TTTTGATTGA GGGAGATTTA AATCCTGATA
  721 ATAGTGATGT GGACAAACTA TTTATCNNGT TGGTACNAAC CTACAATCAA TTATTTGAAG
  781 AAAACCCTAT TAACGCAAGT GGAGTAGATG CTAAAGCGAT TCTTTCTGCN CGATTGANTA
  841 AATCAAGACG ANTAGAAAAA TCTCATTGCT CANCTCCCCG GTGNNAANAA AAANTGGCTN
  901 ATTTGGGANT CTCATTGCTT TGNNNATNGG GTTNGACCCC TAATTTTAAA NCAAATTTTT
  961 GATTTGGCNG ANNNNGCTNA NNTNNCNNCT TTNNAAANNA TACTTNCNAT GANGATTNNA
1021 TNANTTTNNN NNNNGNNNAA NNNNNNANNN NNNNNNN
//
Primer: cas9-B_F (Pr1256)
Region: insert 3',SV40 NLS,T7 pro
Sequence file: RDB12994_A4Jnb.seq check
>12994_12994_B2JeF_3_cas9-B_F_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNAGCTGGC TCTGCNNAGC AAATATGTGA ATTTTTTATA
   61 TTTAGCTAGT CATTATGAAA AGTTGAAGGG TAGTCCAGAA GATAACGAAC AAAAACAATT
  121 GTTTGTGGAG CAGCATAAGC ATTATTTAGA TGAGATTATT GAGCAAATCA GTGAATTTTC
  181 TAAGCGTGTT ATTTTAGCAG ATGCCAATTT AGATAAAGTT CTTAGTGCAT ATAACAAACA
  241 TAGAGACAAA CCAATACGTG AACAAGCAGA AAATATTATT CATTTATTTA CGTTGACGAA
  301 TCTTGGAGCT CCCGCTGCTT TTAAATATTT TGATACAACA ATTGATCGTA AACGATATAC
  361 GTCTACAAAA GAAGTTTTAG ATGCCACTCT TATCCATCAA TCCATCACTG GTCTTTATGA
  421 AACACGCATT GATTTGAGTC AGCTAGGAGG TGACGGTTCT CCCAAGAAGA AGAGGAAAGT
  481 CTCGAGCGGT GGAGCTGCAG GATGAAATTC GATATCTAAC CGACTCCACT CCCACACAAT
  541 TAAGACAATG CTTGGTGATT TGGACTTTTG GCTAAATTAT ATAATAAAAA TTTGAAAAGG
  601 TTGATATAAT AAACTAATTT GAAAAGCTAA AAAAAAAAAA AAAAAACTCG AGGCGGCCGC
  661 GGTACCCAAT TCGCCCTATA GTGAGTCGTA TTACAATTCA CTGGCCGTCG TTTTACAACG
  721 TCGTGNCTGG GAAAACCCTG NCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT
  781 CGCCAGCTGG CGTANTANCG AANAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG
  841 CCNGAATGGN NNANGNNAAN NTGTANNCGT NAATANTTTG TTAAAATTCN CGNNNAATTT
  901 TTGNNAANTC ANCTCATTTT TTNNNNNNNA NGNNNNANNN NGGNNAAANN NNNANNANNC
961 AAANNNNNNN NNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sasaki, H., CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis. Dev. Growth Differ. 56 (7): 499-510 (2014). PMID 25212715. [link to RRC of NBRP]