RDB12999 - pSPCiEF1>Cas9

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pSPCiEF1>Cas9 (#RDB12999)

Expression plasmid of Cas9

Clone info.	Expression plasmid of Cas9. The expression of Cas9 is under the control of Ciona EF1alpha promoter.
Comment	See Fig. 4A of Develop. Growth Differ. (2014) 56, 499-510.
Vector backbone	pSP1.72 (Plasmid)
Size of vector backbone	2.5 kb
Selectable markers	Amp^r
Gene/insert name	Streptococcus pyogenes Cas9 genomic DNA Ciona intestinalis EF1 alpha genomic DNA SV40 genomic DNA
Depositor\|Developer	Sasakura, Yasunori \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Dev Growth Differ., 56, 499-510(2014)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.

Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Dev Growth Differ., 56, 499-510(2014)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks	The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Dev Growth Differ., 56, 499-510(2014))。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
備考	本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB12999	pSPCiEF1>Cas9	Under QC test. Please contact us.	DNA solution	Please contact us.

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pSPCiEF1>Cas9 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12999).

Reference section:

Sasaki, H., Yoshida, K., Hozumi, A., Sasakura, Y., CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis. Dev. Growth Differ. 56 (7): 499-510 (2014). PMID 25212715. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original	Sasaki, H., CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis. Dev. Growth Differ. 56 (7): 499-510 (2014). PMID 25212715. [link to RRC of NBRP]