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pGEX6P-eRF1

Expression vector of human eRF1

Catalog number RDB13269
Resource name pGEX6P-eRF1
Clone info. Expression vector of human eRF1.
Comment Expression was confirmed by depositor with Western blot and CBB staining.
Vector backbone pGEX6P (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name Human eRF1 cDNA
Depositor|Developer Imataka, Hiroaki |
Other clones in our bank

External Database
Human eRF1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Biol Chem. 289(46): 31960-71, 2014)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Biol Chem. 289(46): 31960-71, 2014)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB13269 pGEX6P-eRF1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13269_A7Drp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F2
          Sequence file: RDB13269_A7Dra.seq check
          >13269_13269_A7Dr_pGEX-F2_A10_01_ABI24.ab1
              1 GCTACTAGTT GTTTTAAAAC GTATTGAGCT ATCCCACAAA TTGATAAGTA CTTGAAATCC
             61 AGCAAGTATA TAGCATGGCC TTTGCAGGGC TGGCAAGCCA CGTTTGGTGG TGGCGACCAT
            121 CCTCCAAAAT CGGATCTGGA AGTTCTGTTC CAGGGGCCCC TGGGATCCCC GGAATTCATG
            181 GCGGACGACC CCAGTGCTGC CGACAGGAAC GTGGAGATCT GGAAGATCAA GAAGCTCATT
            241 AAGAGCTTGG AGGCGGCCCG CGGCAATGGC ACCAGCATGA TATCATTGAT CATTCCTCCC
            301 AAAGACCAGA TTTCACGAGT GGCAAAAATG TTAGCGGATG AGTTTGGAAC TGCATCTAAC
            361 ATTAAGTCAC GAGTAAACCG CCTTTCAGTC CTGGGAGCCA TTACATCTGT ACAACAAAGA
            421 CTCAAACTTT ATAACAAAGT ACCTCCAAAT GGTCTGGTTG TATACTGTGG AACAATTGTA
            481 ACAGAAGAAG GAAAGGAAAA GAAAGTCAAC ATTGACTTTG AACCTTTCAA ACCAATTAAT
            541 ACGTCATTGT ATTTGTGTGA CAACAAATTC CATACAGAGG CTCTTACAGC ACTACTTTCA
            601 GATGATAGCA AGTTTGGATT CATTGTAATA GATGGTAGTG GTGCACTTTT TGGCACACTC
            661 CAAGGAAACA CAAGAGAAGT CCTGCACAAA TTCACTGTGG ATCTCCCAAA GAAACACGGT
            721 AGAGGAGGTC AGTCAGCCTT GCGTTTTGCC CGTTTAAGAA TGGAAAAGCG ACATAACTAT
            781 GTTCGGAAAG TAGCAGAGAC TGCTGTGCAG CTGTTTATTT CTGGGGACAA AGTGAATGTG
            841 GCTGGTCTAG TTTTAGCTGG ATCCGCTGAC TTTAAAACTG AACTAAGTCA ATCTGATATG
            901 TTTGATCAGA GGTTACAATC AAAAGTTTTA AAATTAGTTG ATATATCCTA TGGTGGTGAA
            961 AATGGATTCA ACCCAAGCTA TTTGAGTTAT CTACTGAAGT CCTCTCTACG TGAAATTCAT
           1021 TCAAGAGAGA AATTATAGGA CGATACTTTG AATGAAATCA GCCAGGGACA CGGGCAAGTT
          1081 ACCTGGTTTT GCCGATTGGA AGGATACACT AAAGGCCTTT GGGAAAATTG GGGCAC
          //
          Primer: pGEX-R
          Sequence file: RDB13269_A7Drb.seq check
          >13269_13269_A7Dr_pGEX-R_B10_04_ABI24.ab1
              1 TCAATAAAGG AAACGCGCGA GGCAGATCGT CAGTCAGTCA CGATGCGGCC GCTCGAGCTA
             61 GTAGTCATCA AGGTCAAAAA ATTCATCGTC TCCTCCTTGG TATTCCATTC CCTGGAAATC
            121 TACTCGGTAC CGCAAGATAC CTCCAATTCC ACCAAATCCT TTCACAAACT GAGACCCTTC
            181 TTGTGATTTA TCTGTGACAA TTTCCAACGT AGCTCCAAAT TTTTTATAGT TGTTAGCAAA
            241 CCATTCCAAC AGGGGCATGC TCTCGATAAG CTCATGTTCC TGTCCGGTCT CTTTGTCTGT
            301 GAAATGAGAT TTATCCTTTT CTTGCTCTGG AGTTAGATAG AGAATTTTCT CCTCTTCTGT
            361 GCCTTGGCAA TGAAGAACAT ATCTCATTAT ATCCAGATTT TCATAGACTA TTAGAATTTC
            421 TACAGCTCCC ATTTCCAAAG CCTTTAGTGT ATCTTCAACG CCAAAACAGT ACTTGCCCGT
            481 GTCCTGGCTG ATTTCATCAA AGTATCGTCC TATTAATTTC TTCTCTTGAA TGAATTTCAC
            541 GTTGGAGAGG ACTTCAGTAG ATAACTCAAT AGCTTGGTTG AATCCATTTT CACCACCATA
            601 GGATATATCA ACTAATTTTA AAACTTTTGA TTGTAACCTC TGATCAAACA TATCAGATTG
            661 ACTTAGTTCA GTTTTAAAGT CAGCGGATCC AGCTAAAACT AGACCAGCCA CATTCACTTT
            721 GTCCCCAGAA ATAAACAGCT GCACAGCAGT CTCTGCTACT TTCCGAACAT AGTTATGTCG
            781 CTTTTCCATT CTTAAACGGG CAAAACGCAA GGCTGACTGA CCTCCTCTAC CGTGTTTCTT
            841 TGGGAGATCC ACAGTGAATT TGTGCAGGAC TTCTCTTGTG TTTCCTTGGA GTGTGCCAAA
            901 AAGTGCACCA CTACCATCTA TTACAATGAA ATCCAAACTT GCTATCATCT GGAAGTAGTG
            961 CTGTAAGGAG CCTCTGTATG GGAATTTGTT TGTCACACAA ATACCATGAC GTATTAATTG
          1021 GTTTGAAAAG
          //
          Primer: pGEX-ptac_up-F
          Sequence file: RDB13269_A7Drc.seq check
          >13269_13269_A7Dr_pGEX-ptac_up-F_C12_09_ABI24.ab1
              1 ATAAGCCGCT CATACGGTTC TGGCAATATT CTGAAATGAG CTGTTGACAA TTAATCATCG
             61 GCTCGTATAA TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGTATTCAT
            121 GTCCCCTATA CTAGGTTATT GGAAAATTAA GGGCCTTGTG CAACCCACTC GACTTCTTTT
            181 GGAATATCTT GAAGAAAAAT ATGAAGAGCA TTTGTATGAG CGCGATGAAG GTGATAAATG
            241 GCGAAACAAA AAGTTTGAAT TGGGTTTGGA GTTTCCCAAT CTTCCTTATT ATATTGATGG
            301 TGATGTTAAA TTAACACAGT CTATGGCCAT CATACGTTAT ATAGCTGACA AGCACAACAT
            361 GTTGGGTGGT TGTCCAAAAG AGCGTGCAGA GATTTCAATG CTTGAAGGAG CGGTTTTGGA
            421 TATTAGATAC GGTGTTTCGA GAATTGCATA TAGTAAAGAC TTTGAAACTC TCAAAGTTGA
            481 TTTTCTTAGC AAGCTACCTG AAATGCTGAA AATGTTCGAA GATCGTTTAT GTCATAAAAC
            541 ATATTTAAAT GGTGATCATG TAACCCATCC TGACTTCATG TTGTATGACG CTCTTGATGT
            601 TGTTTTATAC ATGGACCCAA TGTGCCTGGA TGCGTTCCCA AAATTAGTTT GTTTTAAAAA
            661 ACGTATTGAA GCTATCCCAC AAATTGATAA GTACTTGAAA TCCAGCAAGT ATATAGCATG
            721 GCCTTTGCAG GGCTGGCAAG CCACGTTTGG TGGTGGCGAC CATCCTCCAA AATCGGATCT
            781 GGAAGTTCTG TTCCAGGGGC CCCTGGGATC CCCGGAATTC ATGGCGGACG ACCCCAGTGC
            841 TGCCGACAGG AACGTGGAGA TCTGGAAGAT CAAGAAGCTC ATTTAAGAGC TTTGGAGGCG
            901 GCCCGCGGCA ATGGCACCAG CATGATATCA TTGATCATTC CTCCCAAAGA CCAGATTTTC
            961 ACGAGTGGCA AAAATGTTAG CGGGATGAGT TTGGGAACTG CATCTAACAT TAAGTCACGA
           1021 GTTAAACCCG CCTTTTCAGT CTGGGAGCAT TTACATTCTT GTTACAACCA AGACTTCAAC
           1081 CTTTATTAAC AAAGTACTCG AAACTGATCT TGCTTGGCTA TTACTTGTGT GGAAACAATT
          1141 TTGTTTAACC AGGAG
          //
          Primer: M13_-40
          Sequence file: RDB13269_A7Drd.seq check
          >13269_13269_A7Dr_M13(-40)_C10_07_ABI24.ab1
              1 CGGCCGCTCA TCGTATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA
             61 ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG
            121 AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG
            181 TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC
            241 TAGCGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG
            301 GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG
            361 TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC
            421 TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG
            481 CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG
            541 CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG
            601 AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA
            661 ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC
            721 GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA
            781 GACATCAAGA AATAACGCCG GAACATTAGT GCAGGCAGCT TCCACAGCAA TGGCATCCTG
            841 GTCATCCAGC GGATAGTTAA TGATCAGCCC ACTGACGCGT TGCGCGAGAA GATTGTGCAC
            901 CGCCGCTTTA CAGGCTTCGA CGCCGCTTCG TTCTACCATC GACACCACCA CGCTGGCACC
            961 CAGTTGATCG GGCGCGAGAT TTAATCGCCG CGACAATTTG CGACGGCGCG TGCAGGCCAG
           1021 ACTGGAGGTG GCACGCGATC AGCACGACTG TTTTGCCGGC AGCGGTGTGC ACGGCGGGAT
          1081 TGGAATGTAA TCAGCTCCGG CCATCGCCGC TTCCAACCAT ATTTCCCCGC GCGAATA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322.
          reference Imataka, H., Advantages of human cell-derived cell-free protein synthesis systems (in Japanese text). SEIKAGAKU 81 (4): 303-307 (2009). PMID 19462838.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl