Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCMV/His tagged-Mettl23/IRES2/EGFP

Expression clone of Methyltransferase like 23 (Mettl23)

Sequence (full)	RDB13316zzk02.seq provided by the depositor

Distribution information

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13316_B1Lep1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, 6xHis, insert 5' Sequence file: RDB13316_B1Lea.seq
>13316_13316_B1Le_1_CMV_Forward_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNCG GTGGGAGGNC TATATAAGCA GAGCTGGTTT AGTGAACCGT    61 CAGATCCGCT AGCGCTACCG GACTCAGATC TCGAGCTCAA GCTTCGAATT CATGCATCAC   121 CATCACCATC ACATGGACAG TGTCCAGCCC CGCGCTCCTT GGGCACCGCC TCCTGACCCG   181 GCGTCCCTGG ACTCCCCTAC GTGCGAGCCC GGCCTCATGG CCGGGACCCA GCTCTTTCGG   241 TTCCGGGAGG AGCCAGTTCC CGGAGGCAAT CGGGCGGTGC TGGAGGTCCG CGTCCCGCAG   301 GTCCTGCATG TCCAGTATGG AATGTATGTT TGGCCCTGTG CTGTAGTCCT GGCCCAATAC   361 CTGTGGTTTC ACAGAAGATC TCTACCAGGC AAAGCTGTCT TAGAGGTTGG CGCTGGAGTG   421 AGCCTTCCTG GGATTTTGGC TGCCAAATGT GGTGCTAAAG TAATACTATC TGACAGCTCA   481 GAGTTTCCTC ACTGTCTGGA TATCTGCAGG CAAAGTTGCC AGATGAATAA CCTGCCACAG   541 GTCGAAGTTG TAGGACTGAC ATGGGGCCAC ATATCANAGG ACATTCTGTC TTTGCCNCCN   601 CAAGACATCN TTCTTGGATC TGATGTGTTC TTTGAACCNG AANANTTTGA AAGCANTTTA   661 GCCNCNGNAT ACTTCCNGAN GCNNAANANN CNNAAANTNC NNNTTNNNNC NNNNTNNNNN 721 NNNNN //
Primer: IRES-R (Pr0037) Region: IRES2, insert 3' Sequence file: RDB13316_B1Leb.seq
>13316_13316_B1Le_1_IRES-R_B01_04_ABI24.ab1     1 NNNNNNNNNN NNGGGGGGGG NNNNNNNNGG GCGGATCCTC AGAAACTGTC CTTTGCAAAG    61 GAGATGATCA GCATTTCAAC AGTATGTCTT CCTGGAAGAG TAGACTCTGC TATGTCTTCC   121 TTGTCGGCAT CAAAAGACTC CAGAGGAATA TGCACACATT TCATATCCCA CGTGTAGAGC   181 AAGCCTTCAA GTGACCAGTC AGCACTTCGG ACCTGGTATG TAGACCAAAA TTGAACTTTT   241 GGATTCTTCT GCATCAGGAA GTATACTGTG GCTAAAATGC TTTCAAAGTC TTCTGGTTCA   301 AAGAACACAT CAGATCCAAG AATGATGTCT TGTGGTGGCA AAGACAGAAT GTCCTTTGAT   361 ATGTGGCCCC ATGTCAGTCC TACAACTTCG ACCTGTGGCA GGTTATTCAT CTGGCAACTT   421 TGCCTGCAGA TATCCAGACA GTGAGGAAAC TCTGAGCTGT CAGATAGTAT TACTTTAGCA   481 CCACATTTGG CAGCCAAAAT CCCAGGAAGG CTCACTCCAG CGCCAACCTC TAAGACAGCT   541 TTGCCTGGTA GAGATCTTCT GTGAAACCAC AGGTATTGGG CCAGGACTAC AGCACANGGC   601 CNAACATACA TTCNNTACTG GACATGCANG ACCTGCGGGN NGCGGANCTC CNGCACCGCC   661 CNANTGCCTC CNGGNNCTGG CTNCTCCCNG ANCCNAAAGA NCTGGNNCCC NGNNNNTNNN 721 NNNNGNNNNN NNNNN //
Primer: EGFP-N_R2 (Pr0905) Region: EGFP 5', IRES2 Sequence file: RDB13316_B1Lec.seq
>13316_13316_B1Le_1_EGFP-N_R2_C01_07_ABI24.ab1     1 NNNNNNNNGG CNNNNCNNTG CACGCCGTAN GTCAGGGTGG TCACGAGGGT GGGCCAGGGC    61 ACGGGCAGCT TGCCGGTGGT GCAGATGAAC TTCAGGGTCA GCTTGCCGTA GGTGGCATCG   121 CCCTCGCCCT CGCCGGACAC GCTGAACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC   181 AGGATGGGCA CCACCCCGGT GAACAGCTCC TCGCCCTTGC TCACCATGGT TGTGGCCATA   241 TTATCATCGT GTTTTTCAAA GGAAAACCAC GTCCCCGTGG TTCGGGGGGC CTAGACGTTT   301 TTTTAACCTC GACTAAACAC ATGTAAAGCA TGTGTACCGA GGCCCCAGAT CAGATCCCAT   361 ACAATGGGGT ACCTTCTGGG CATCCTTCAG CCCCTTGTTG AATACGCTTG AGGAGAGCCA   421 TTTGACTCTT TCCACAACTA TCCAACTCAC AACGTGGCAC TGGGGTTGTG CCGCCTTTGC   481 AGGTGTATCT TATACACGTG GCTTTTGGCC GCAGAGGCAC CTGTCGCCAG GTGGGGGGTT   541 CCGCTGCCTG CAAAGGGTCG CTACAGACGT TGTTTGTCTT CAAGAAGCTT CCAGAGGAAC   601 TGCTTCCTTC ACGACATTCA ACAGACCTTG CATTCCTTTG GCGAGANGGG NAAAGANCCC   661 TAGGAATGNT CGTCNNGANN ACAGGNNNNN GGTTTCCNGG NCNNCACATT GNNNAANNAN 721 NGNNNNANNG NNGAAAANNN NNNNNNN //
Primer: mVenus-M_F (Pr0574) Region: EGFP 3', SV40 pA, f1 ori Sequence file: RDB13316_B1Led.seq
>13316_13316_B1Le_1_mVenus-M_F_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGTG NACTTNNAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC    61 AGCTCGCCGA CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG   121 ACAACCACTA CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC   181 ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT   241 ACAAGTAAAG CGGCCGCGAC TCTAGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT   301 ACTTGCTTTA AAAAACCTCC CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT   361 TGTTGTTGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC   421 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT   481 CAATGTATCT TAAGGCGTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT   541 TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATANNN   601 NNNAAGAATA GACCGAGATA GGGTTGAGTG NTGTTCCNGT TTGGAACNAG AGTCCACTAT   661 TAAANAACGT GGACTNCNAC GTCNANNGGC GAAAANNNGT CTATCNNNNN NNNGNNNNNN 721 NCNNNNNNNN TCNCCNNNNN NNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, Neo_KanR Sequence file: RDB13316_B1Lee.seq
>13316_13316_B1Le_1_SV40pro_ori_F_E01_13_ABI24.ab1     1 NNNNNNNNNC TCNGCCTCTN NNNNNTTCNN GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT    61 AGGCTTTTGC AAAGATCGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG   121 ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG   181 CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC   241 CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAA GACGAGGCAG   301 CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA   361 CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT   421 CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA   481 CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC   541 GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC   601 TCGCGCCNGC CGAACTGTTC GCCNGGCTCN AGGCGAGCAT GCCCGACNGC GANGNNCTCG   661 TCGNGACCNN TGGNNANGCC TGCTNGNCNA ATANNNTGGN NGAAAATGNN NNCTTTNNNN 721 NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl