Please review the QC test results indicated by icon below as well as clone information before placing your order.

pONL-N1

Orange Nano-lantern to construct fusion proteins to the N-terminus.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内) [open/close]

提供条件	本件リソースの利用は、非営利機関による学術研究に限る。利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。利用者は、mKO2について理化学研究所CBS 宮脇チームから、書面による提供承諾を得ること。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	宮脇敦史博士の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13327_A5E8p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro,insert 5' Sequence file: RDB13327_A5E8a.seq
>13327_13327_A5E8_3_CMV_Forward_D03_12.ab1     1 NNNNNNNNNN NNNNTNNNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTCGAGCTC   121 AAGCTTCGAA TTCTGCAGTC GACGGTACCG CGGGCCCGGG ATCCACCGGT CGCCACCATG   181 GTGAGCGTGA TCAAGCCCGA GATGAAGATG AGGTACTACA TGGACGGCTC CGTCAATGGG   241 CATGAGTTCA CAATCGAGGG TGAGGGCACA GGCAGACCTT ACGAGGGACA TCAGGAGATG   301 ACACTGCGCG TCACAATGGC CGAGGGCGGG CCAATGCCTT TCGCCTTCGA CCTGGTGTCC   361 CACGTGTTCT GTTACGGCCA CAGAGTTTTT ACTAAATATC CAGAAGAGAT CCCAGACTAT   421 TTCAAGCAGG CCTTTCCTGA GGGCCTGTCC TGGGAGAGGT CCCTGGAGTT CGAGGACGGC   481 GGCTCCGCCT CCGTGAGCGC CCACATCAGC CTGAGGGGCA ACACCTTCTA CCACAAGTCC   541 AAGTTCACCG GCGTGAACTT CCCCGCCGAC GGCCCCATCA TGCAGAACCA GAGCGTGGAC   601 TGGGAGCCCT CCACCGAGAA GATCACCGCC AGCGACGGCG TGCTGAAGGG CGACGTGACC   661 ATGTACCTGA AGCTGGAGGG CGGCGGCAAC CACAAGTGCC AGATGAAGAC CACCTACAAG   721 GCCGCCAAGG AGATCCTGGA GATGCCCGGC GACCACTACA TCGGCCACAG GCTGGTGAGG   781 AAGANCGAGG GCAACATCAC CGANCANGTG GANGANGCCG TGGCCCACTC CNGTACCNAN   841 GTGTACGACN NCGANCAACG CAAACGCATG ATCNCTGGGC CTCANNNNNG GGCTCGCTGC   901 NAGCAAATGA NCGNGCNNNN NNNCTTCNTC NNNNACNATN NNTNCNNNNA NNCNCGNNNN 961 NNNNNN //
Primer: SV40polyA_R2 Region: insert 3' Sequence file: RDB13327_A5E8b.seq
>13327_13327_B0IiF_3_SV40polyA_R2_E11_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNTTNN NNANNNNNNN NNTGCATTNN NTTTANGTTT NNGGTTCNGG    61 GGGANGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT   121 ATGATCTAGA GTCGCGGCCG CCTACTGCTC GTTCTTCAGC ACGCGCTCCA CGAAGCTCTT   181 GATGTACTTA CCCATTTCAT CTGGAGCGTC CTCCTGGAGG AAGTGGAGGC CCTTCACCTT   241 CACGAACTCG GTGTTAGGGA ACTTCTTAGC TCCCTCGACA ATAGCGTTGG AAAAGAACCC   301 AGGGTCGGAC TCGATGAACA GCTTAGGCAG ATCGTCGCTG GCCCGAAGGT AGGCGTTGTA   361 GTTGCGGACA ATCTGGACGA CGTCGGGCTT GCCTCCCTTA ACGAGAGGGA TCTCGCGAGG   421 CCAGGAGAGG GTAGGCCGTC TAACCTCGCC CTTCTCCTTG AATGGCTCCA GGTAGGCAGC   481 GAACTCCTCA GGCTCCAGTT TCCGCATGAT CTTGCTTGGC AACAGGGTCT CGACGAAGAA   541 GTTATTCTCA AGCACCATTT TCTCGCCCTC TTCGCTCTTG ATCAGGGCCA GCTCCTCCTC   601 GATGTCAGGC CACCCCATCC AGGACTCGAT CACGTCCACG ACACTCTCCA TATGGACGAT   661 GGCCTTGATC CTGTCTTGNT GCTCGTANGC GTAGTGAAAG GNNAGAGCGC TCCCCCANTC   721 GTGGCCCACA AAGATGATTT TCTTTGGAAN NGTTCANCAG CTCNAACCAA GCGGTGAGGT   781 ACTTGNANTG ATCCNNGNAG GCGATATGAN CCATTCCCGC TCTTGCCGGA NTTACCCATT   841 CCNATCAGAT CNNGNNTGAN GCNTCTNNNC NNCNGGGCNC GNNNGNNAGG CANNNNNNGN 901 CNTNNNNNNN NNN //
Primer: mKO2-M_F Region: insert mid Sequence file: RDB13327_A5E8c.seq
>13327_13327_B0IiF_3_mKO2-M_F_C02_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGAN GAAGNNNNCC TACNAGGCCG CNNAGGAGNT    61 CCTGGAGANG CCCGGCGACC ACTACATCGG CCACAGGCTG GTGAGGAAGA CCGAGGGCAA   121 CATCACCGAG CAGGTGGAGG ACGCCGTGGC CCACTCCGGT ACCAAGGTGT ACGACCCCGA   181 GCAACGCAAA CGCATGATCA CTGGGCCTCA GTGGTGGGCT CGCTGCAAGC AAATGAACGT   241 GCTGGACTCC TTCATCAACT ACTATGATTC CGAGAAGCAC GCCGAGAACG CCGTGATTTT   301 TCTGCATGGT AACGCTACCT CCAGCTACCT GTGGAGGCAC GTCGTGCCTC ACATCGAGCC   361 CGTGGCTAGA TGCATCATCC CTGATCTGAT CGGAATGGGT AAGTCCGGCA AGAGCGGGAA   421 TGGCTCATAT CGCCTCCTGG ATCACTACAA GTACCTCACC GCTTGGTTCG AGCTGCTGAA   481 CCTTCCAAAG AAAATCATCT TTGTGGGCCA CGACTGGGGG AGCGCTCTGG CCTTTCACTA   541 CGCCTACGAG CACCAAGACA GGATCAAGGC CATCGTCCAT ATGGAGAGTG TCGTGGACGT   601 GATCGAGTCC TGGATGGGGT GGCCTGACAT CGAGGAGGAG CTGGCCCTGA TCAAGAGCGA   661 AGAGGGCGAG AAAATGGTGC TTGAGAATAA CTTCTTCGTC GAGACCCTGT TGCCAAGCAA   721 GATCATGCGG AANCTGGAGC CTGAGGAGTT CGCTGCCTAC CTGGAGCCAT TCAAGGAGAA   781 GGGCGAGGTT AGACGGCCTA CCCTCTCCTG GNCNTCGCGA GATCCCTCTC GTTAANGGAG   841 GCANNCCCGA CNTCGTCNAG ATTGTCCGCA ANNNCNNCGC CTACCTTCGG GNCAGCNNNN   901 NATNTGCCNN AANCTGTNNN TCNAGTCCNA NCCNGGGNNC NTTNNCNANN NCTNNNGTCG   961 ANGGGANCNN AAGAANTTNC CNTAACNCCN NNNNNGTGNA NNNTNNAANG NCNNCNNNNT 1021 NNNNNNNNNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori,NeoR_KanR Sequence file: RDB13327_A5E8d.seq
>13327_13327_B0IiF_3_SV40pro_ori_F_G11_20_ABI24.ab1     1 NNNNNNNNNN NNNNNNTGAN CTNNTCNNGA AGTAGTGAGG ANGCTTTTTT GGAGGCCTAG    61 GCTTTTGCAA AGATCGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT   121 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA   181 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG   241 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAAGA CGAGGCAGCG   301 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT   361 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT   421 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG   481 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT   541 ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC   601 GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC CCGACGGCGA GGATCTCGTC   661 GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA   721 TTCATCGACT GTGGCCGGCT GGGTGTGGCG GANCGCTATC ANGACATAGC GTTGGCTACC   781 CGNGATATTG CTGAANANCT NGGCGGCGAA TGGGCTGACC GCTTCCTCNN GCTTTNCNGN   841 ATCNCNNCTC CCNATTCNCA NCNNATCNNC NNTCNATCNN CNNTCTNNNN NANNTNNNNN 901 NNANNGGNNN NNNNGGNNNT NNNNANNNNN NNANNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl