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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pONL-C1

Orange Nano-lantern to construct fusion proteins to the C-terminus.

Catalog number RDB13328
Resource name pONL-C1
Clone info. Cloning and expression vector to construct fusion proteins to the C-terminus of Orange Nano-lantern.
Comment Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. ONL contains a portion of hmKusabiraOrange2 provided by Dr. Atsushi Miyawaki, the RIKEN BSI.
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli)
Depositor|Developer Okada, Yasushi | Takai, Akira |
Other clones in our bank

External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 本件リソースの利用は、非営利機関による学術研究に限る。利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。利用者は、mKO2について理化学研究所CBS 宮脇チームから、書面による提供承諾を得ること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            備考
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB13328 pONL-C1 DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Original reference

            original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Nano-lantern (English text)
            Featured content Nano-lantern (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13328_B0Ihp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward
            Region: CMV pro,insert 5'
            Sequence file: RDB13328_B0Iha.seq check
            >13328_13328_B0Ih_CMV-Forward_F01_16_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNGGCGG TANGCGTGTN NNGGTGGGNN GTCTATATAA
               61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCCA CCATGGTGAG CGTGATCAAG
              121 CCCGAGATGA AGATGAGGTA CTACATGGAC GGCTCCGTCA ATGGGCATGA GTTCACAATC
              181 GAGGGTGAGG GCACAGGCAG ACCTTACGAG GGACATCAGG AGATGACACT GCGCGTCACA
              241 ATGGCCGAGG GCGGGCCAAT GCCTTTCGCC TTCGACCTGG TGTCCCACGT GTTCTGTTAC
              301 GGCCACAGAG TTTTTACTAA ATATCCAGAA GAGATCCCAG ACTATTTCAA GCAGGCCTTT
              361 CCTGAGGGCC TGTCCTGGGA GAGGTCCCTG GAGTTCGAGG ACGGCGGCTC CGCCTCCGTG
              421 AGCGCCCACA TCAGCCTGAG GGGCAACACC TTCTACCACA AGTCCAAGTT CACCGGCGTG
              481 AACTTCCCCG CCGACGGCCC CATCATGCAG AACCAGAGCG TGGACTGGGA GCCCTCCACC
              541 GAGAAGATCA CCGCCAGCGA CGGCGTGCTG AAGGGCGACG TGACCATGTA CCTGAAGCTG
              601 GAGGGCGGCG GCAACCACAA GTGCCAGATG AAGACCACCT ACAAGGCCGC CAAGGAGATC
              661 CTGGAGATGC CCGGCGACCA CTACATCGGC CACAGGCTGG TGAGGAAGAN CGANGGCAAC
              721 ATCACCGANC AGGTGGANGA NGCCGNGGCC CACTCCGGTA CCAAGGTGTA CGACCCCGAN
              781 CAACGCAAAC GCATGATCAC TNNNCCTCAN NGGTGGGCTC GCTGCAAGCA AATGAACGNG
              841 CNNGANTCCT NCATCNACTA CTATNNNTNN NANAANCACG CNNNNAACGC CNNNNTTTTT
            901 CNGNNTGGNN ACGCTACNNC CNNCTNNCNN NNNGNNNNNN NNNN
            //
            Primer: SV40polyA_R2
            Region: insert 3'
            Sequence file: RDB13328_B0Ihb.seq check
            >13328_13328_B0Ih_SV40polyA_R2_F02_17_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNGTTNACAN CAACAATTGC ATTNNTTTTA TGTTTCAGGT
               61 TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT GTGGTATGGC
              121 TGATTATGAT CAGTTATCTA GATCCGGTGG ATCCCGGGCC CGCGGTACCG TCGACTGCAG
              181 AATTCGAAGC TTGAGCTCGA GATCTGAGTC CGGACTGCTC GTTCTTCAGC ACGCGCTCCA
              241 CGAAGCTCTT GATGTACTTA CCCATTTCAT CTGGAGCGTC CTCCTGGAGG AAGTGGAGGC
              301 CCTTCACCTT CACGAACTCG GTGTTAGGGA ACTTCTTAGC TCCCTCGACA ATAGCGTTGG
              361 AAAAGAACCC AGGGTCGGAC TCGATGAACA GCTTAGGCAG ATCGTCGCTG GCCCGAAGGT
              421 AGGCGTTGTA GTTGCGGACA ATCTGGACGA CGTCGGGCTT GCCTCCCTTA ACGAGAGGGA
              481 TCTCGCGAGG CCAGGAGAGG GTAGGCCGTC TAACCTCGCC CTTCTCCTTG AATGGCTCCA
              541 GGTAGGCAGC GAACTCCTCA GGCTCCAGTT TCCGCATGAT CTTGCTTGGC AACAGGGTCT
              601 CGACGAAGAA GTTATTCTCA AGCACCATTT TCTCGCCCTC TTCGCTCTTG ATCAGGGCCA
              661 GCTCCTCCTC GATGTCANGC CACCCCATCC AGGACTCGAT CACGTCCACG ACACTCTCCA
              721 TATGGACGAT GGCCTTGATC CTGTCTTGGT GCTCGTANGC GTANTGAAAG GCCAGANCGC
              781 TCCCCCAGTC GTGGCCCACA AAGATGANTT TCTNNGNAAN GGNTCAGCAG CTCGAANNNA
              841 NCGGNNNNGT ACTTGNANNG ATCCAGGANG NNATATGANC NATTCCNGCT CNNGNNNNNN
            901 NTANCCNTTC CNATCANNNC NNNNNNNNNN
            //
            Primer: mKO2-M_F
            Region: insert mid
            Sequence file: RDB13328_B0Ihc.seq check
            >13328_13328_B0Ih_mKO2-M_F_B06_06_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN CNANNGCNNG ATGAAGNNCA CCTACNAGGC CGCCNAGGAG
               61 ATCCTGGAGA TGCCCGGCGA CCACTACATC GGCCACAGGC TGGTGAGGAA GACCGAGGGC
              121 AACATCACCG AGCAGGTGGA GGACGCCGTG GCCCACTCCG GTACCAAGGT GTACGACCCC
              181 GAGCAACGCA AACGCATGAT CACTGGGCCT CAGTGGTGGG CTCGCTGCAA GCAAATGAAC
              241 GTGCTGGACT CCTTCATCAA CTACTATGAT TCCGAGAAGC ACGCCGAGAA CGCCGTGATT
              301 TTTCTGCATG GTAACGCTAC CTCCAGCTAC CTGTGGAGGC ACGTCGTGCC TCACATCGAG
              361 CCCGTGGCTA GATGCATCAT CCCTGATCTG ATCGGAATGG GTAAGTCCGG CAAGAGCGGG
              421 AATGGCTCAT ATCGCCTCCT GGATCACTAC AAGTACCTCA CCGCTTGGTT CGAGCTGCTG
              481 AACCTTCCAA AGAAAATCAT CTTTGTGGGC CACGACTGGG GGAGCGCTCT GGCCTTTCAC
              541 TACGCCTACG AGCACCAAGA CAGGATCAAG GCCATCGTCC ATATGGAGAG TGTCGTGGAC
              601 GTGATCGAGT CCTGGATGGG GTGGCCTGAC ATCNNNNAGG AGCTGGCCCT GATCAAGAGC
              661 GAAGAGGGCG AGAAAATGGT GCTTGAGAAT AACTTCTTCG TCGAGACCCT GTTGCCAAGC
              721 AAGATCATGC GGAAACTGGA GCCTGAGGAG TTCGCTGCCT ACCTGGAGCC ATTCNAGGAG
              781 AANGGCGAGG TTAGACGGCC TACCCTCTCC TGNNCTCGCG AGATCCCTCT CGTTAANGGA
              841 GGCAAGCCCG ACGNCGTCCA GATTGTCCGC AACTACNANN GCCTACCTTC NGGCCNNNCG
              901 NCNATCTGCC NNAGCTGTNC ANCGAGTCCN ANCCNGGGGN TCTTTTNCNA NCNCTANTGT
              961 CNAGGGAGCN NANAANNNTC CCTAANNNNC NANNTTNNNN AANGNNAANG GNNCNNNNNN
            1021 NTNCNNNCAG GNANGNNGCT NNNNNANNAA AATGGGGNNN NNNNNNN
            //
            Primer: SV40pro_ori_F
            Region: SV40 pro_ori,NeoR_KanR
            Sequence file: RDB13328_B0Ihd.seq check
            >13328_13328_B0Ih_SV40pro_ori_F_C06_09_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNGCCTCNNC CTCTGANCTA TTCNNGAAGT AGTGAGGAGG
               61 CTTTTTTGGA GGCCTAGGCT TTTGCAAAGA TCGATCAAGA GACAGGATGA GGATCGTTTC
              121 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT
              181 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT
              241 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC
              301 TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG
              361 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC
              421 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA
              481 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC
              541 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG
              601 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG
              661 ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA
              721 ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAN CGCTATCAGG
              781 ACATAGCGTT GGCTACCCGN GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGANCGCT
              841 TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCNCAGCG CATCGCNNTC TATCGCCTTC
              901 TTGACNANTT CTTCTGANCG GGNNTCTGGG GGTNNGAAAT GNNCGANNNN NCGACGCCCA
              961 ANCNNGNCAT CNCNAGANNN NGATTNNNNC NNNNNNCNTT CNANNAANNG NNNGGNNNNN
            1021 NNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.
            reference Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl