Please review the QC test results indicated by icon below as well as clone information before placing your order.

pYNL-N1_TUBB5

Yellow Nano-lantern for labeling of beta-tubulin by the mouse beta-tubulin class V.

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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提供条件	利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。本件リソースの利用は、非営利機関による学術研究に限る。
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13338_A5E8p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5'(mouse Tubb5) Sequence file: RDB13338_A5E8a.seq
>13338_13338_B1G9F_3_CMV_Forward_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNGNN NNNNNGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCGCTA CCGGACTCAG ATCTCGAGCT   121 CAAGCTTCGA ATTCCACCAT GAGGGAAATC GTGCACATCC AGGCCGGACA GTGTGGCAAC   181 CAGATCGGTG CTAAGTTCTG GGAGGTGATA AGCGATGAAC ATGGCATCGA CCCCACCGGT   241 ACCTACCACG GTGACAGCGA CCTGCAGCTG GACCGAATCT CTGTGTACTA TAATGAAGCC   301 ACAGGTGGCA AGTATGTCCC TCGAGCTATC TTGGTGGATC TAGAACCTGG GACTATGGAC 361 TCCGTTCGCT CAGGTCCTTT TGGNCNNATC TTCNNANNAG ACNNCTTCNN NNNNNNNN //
Primer: Rluc8-C_F2 (Pr1028) Region: insert 3'(Rluc8-m1 dN3),SV40 pA,f1 ori Sequence file: RDB13338_A5E8b.seq
>13338_13338_B1G9F_3_Rluc8-C_F2_D02_11_ABI24.ab1     1 NNNNNNNNGN NNGNNGAAGG GCCTGCACTT CCTCCNGGAG GACGCCCCCG ACGAGATGGG    61 CAAGTACATC AAGAGCTTCG TGGAGAGAGT GCTGAAGAAC GAGCAGTAGG CGGCCGCGAC   121 TCTAGATCAT AATCAGCCAT ACCACATTTG TAGAGGTTTT ACTTGCTTTA AAAAACCTCC   181 CACACCTCCC CCTGAACCTG AAACATAAAA TGAATGCAAT TGTTGTTGTT AACTTGTTTA   241 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT   301 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TAAGGCGTAA   361 ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT   421 TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA   481 GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC   541 GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCTAA   601 TCNAGTTTTT TGGGGNCNAN GTGCCGTAAA GCACTAAATC GGAANCCTAA AGGGANCCCC   661 CGANTTNNAN CTNGNNGGGN NAANNNNNNN ANNNNNNNAN NANNNNNNNN NANNNNNANN 721 NNNNNGNNNN NNNNNN //
Primer: Venus-N_R (Pr0341) Region: insert mid(mVenus dC10,mouse Tubb5) Sequence file: RDB13338_A5E8c.seq
>13338_13338_B1G9F_3_Venus-N_R_A02_02_ABI24.ab1     1 NNNNNNNCNN NNNTTNTGGC CGTTNNCGTC GCCGTCNNGC TCGACNNGGA TGGGCACCAC    61 CCCGGTGAAC AGCTCCTCGC CCTTGCTCAC CATGGTGGCG ACCGGTGGAT CCCGGGCCCG   121 CGGTACCGTC GACTGGGCCT CCTCTTCTGC CTCCTCTCCG AAATCCTCTT CCTCTTCCGC   181 GGTGGCATCC TGGTACTGCT GGTACTCAGA GACGAGGTCG TTCATGTTGC TCTCAGCTTC   241 GGTGAACTCC ATCTCGTCCA TGCCCTCACC CGTATACCAG TGGAGGAAAG CCTTCCGGCG   301 GAACATAGCC GTAAACTGCT CAGAGATGCG CTTGAAGAGC TCCTGGATGG CTGTGCTGTT   361 TCCAATGAAG GTGACTGCCA TCTTGAGGCC ACGCGGTGGG ATGTCACAGA CAGCTGTCTT   421 GACATTGTTG GGGATCCATT CCACNAAGTA GCTGCTATTC TTGTTCTGCA CGTTGAGCAT 481 CTGCTCATCC ACCTCCTTCA TGGACATCCG TCCACGGAAG ANNNNNNNNN N //
Primer: Rluc8-H_R (Pr1031) Region: insert mid(Rluc8-m1 dN3,mVenus dC10) Sequence file: RDB13338_A5E8d.seq
>13338_13338_B1G9F_3_Rluc8-H_R_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NTCCTCTTCC TCTGCTCGGG GTCGTACACC TTGGTACCCC    61 CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC   121 TCAGCTTGGA CTGGTAGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA   181 TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCC GCCGTCCTCG ATGTTGTGGC   241 GGATCTTGAA GTTGGCCTTG ATGCCGTTCT TCTGCTTGTC GGCGGTGATA TAGACGTTGT   301 GGCTGTTGTA GTTGTACTCC AGCTTGTGCC CCAGGATGTT GCCGTCCTCC TTGAAGTCGA   361 TGCCCTTCAG CTCGATGCGG TTCACCAGGG TGTCGCCCTC GAACTTCACC TCGGCGCGGG   421 TCTTGTAGTT GCCGTCGTCC TTGAAGAAGA TGGTGCGCTC CTGGACGTAG CCTTCGGGCA   481 TGGCGGACTT GAAGAAGTCG TGCTGCTTCA TGTGGTCGGG GTAGCGGGCG AAGCACTGCA   541 GGCCGTAGCC CAGGGTGGTC ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC   601 AGATCAGCTT CAGGGTCNGC TTGCCGTANG TGNNATCGCC CTCGCCCTCG CCGGANACGC   661 TGAACTTGTG GNCNNTNACG TCNCNGTCNN NCTCNANCNN GANGGNNANN NNCCNNNNNA 721 NNGCNNNTNN CCNNNNNNNN NN //
Primer: tube-B_F (Pr1030) Region: insert mid(mouse Tubb5,mVenus dC10) Sequence file: RDB13338_A5E8e.seq
>13338_13338_B1G9F_3_tube-B_F_A02_02_ABI24.ab1     1 NNNNNNNGNN NNNNNNNGAG GNNTTCGGAG AGGAGGCAGA AGAGGAGGCC CAGTCGACGG    61 TACCGCGGGC CCGGGATCCA CCGGTCGCCA CCATGGTGAG CAAGGGCGAG GAGCTGTTCA   121 CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG   181 TGTCCGGCGA GGGCGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG CTGATCTGCA   241 CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC CACCCTGGGC TACGGCCTGC   301 AGTGCTTCGC CCGCTACCCC GACCACATGA AGCAGCACGA CTTCTTCAAG TCCGCCATGC   361 CCGAAGGCTA CGTCCAGGAG CGCACCATCT TCTTCAAGGA CGACGGCAAC TACAAGACCC   421 GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG CATCGAGCTG AAGGGCATCG   481 ACTTCAAGGA GGACGGCAAC ATCCTGGGGC ACAAGCTGGA GTACAACTAC AACAGCCACA   541 ACGTCTATAT CACCGCCGAC AAGCAGAAGA ACGGCATCAA GGCNAACTTC AAGATCCGCC   601 ACAACATCGA GGANGGCGGC GTGCAGCTCG CCGANCACTA CCAGCAGAAC ACCCCCATCG   661 GCGANNGCCC CNNGCTGCTG CCCGANNNCC NCTANNNGAN CTNNNNGTCC NNGCNNANNN 721 ANNNNCCNNN NNNNNNN //
Primer: Rluc8-B_R (Pr1032) Region: insert mid(Rluc8-m1 dN3,mVenus dC10) Sequence file: RDB13338_A5E8f.seq
>13338_13338_B1G9F_3_Rluc8-B_R_C02_08_ABI24.ab1     1 NNNNNNNNNN NCTTTCTNNT GATCTTGCTG GGCAGCACGG TCTCCACGAA GAAGTTGTTC    61 TCCAGCACCA TCTTCTCGCC CTCCTCGCTC TTGATCAGGG CGATGTCCTC CTCGATGTCT   121 GGCCACTCGT CCCAGCTCTC GATCACGTCC ACCACGCTCT CCATGTGCAC GATGGCCTTG   181 ATCCTGTCCT GGTGCTCGTA GGCGTAGTGG AAGGCCAGGG CGGCGCCCCA GTCGTGGCCC   241 ACGAAGATGA TCTTCTTGGG CAGGTTCAGA AGCTCGAACC AGGCGGTCAG GTACTTGTAG   301 TGGTCCAGCA GCCTGTAGCT GCCGTTGCCG CTCTTGCCGC TCTTGCCCAT GCCGATCAGA   361 TCGGGGATGA TGCACCTGGC CACGGGCTCG ATGTGGGGCA CCACGTGCCT CCACAGGTAG   421 CTGCTAGTGG CGTTGCCGTG CAGGAAGATC ACGGCGTTCT CGGCGTGCTT CTCGCTGTCG   481 TAGTAGTTGA TGAAGCTGTC CAGCACGTTC ATCTGCTTGC ACCTGGCCCA CCACTGGGGG   541 CCGGTGATCA TCCTCTTCCT CTGCTCGGGG TCGTACACCT TGGTACCCCC GGCGGCGGTC   601 ACGAACTCCA GCANGANCNT GTGATCGCGC TTCTCGTTGG GGTCNTTGCT CANCTTGGAN   661 TGNTANCTCN NNNANNNNNN GNCNGGCANC ANCACNGGGN NNNCNNNNAN GGGGGNNNNC 721 NGNNGGNNNN NNNNNN //
Primer: Rluc8-M_F (Pr0580) Region: insert mid(Rluc8-m1 dN3) Sequence file: RDB13338_A5E8g.seq
>13338_13338_B1G9F_3_Rluc8-M_F_20_H01_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCCNNNGAC NGGNTCAAGG CCATCGTGCA    61 CATGGAGAGC GTGGTGGACG TGATCGAGAG CTGGGACGAG TGGCCAGACA TCGAGGAGGA   121 CATCGCCCTG ATCAAGAGCG AGGAGGGCGA GAAGATGGTG CTGGAGAACA ACTTCTTCGT   181 GGAGACCGTG CTGCCCAGCA AGATCATGAG AAAGCTGGAG CCCGAGGAGT TCGCCGCCTA   241 CCTGGAGCCC TTCAAGGAGA AGGGCGAGGT GAGAAGACCC ACCCTGAGCT GGCCCAGAGA   301 GATCCCCCTG GTGAAGGGCG GCAAGCCCGA CGTGGTGCAG ATCGTGAGAA ACTACAACGC   361 CTACCTGAGA GCCAGCGACG ACCTGCCCAA GCTGTTCATC GAGGGCGACC CCGGCTTCTT   421 CAGCAACGCC ATCGTGGAGG GCGCCAAGAA GTTCCCCAAC ACCGAGTTCG TGAAGGTGAA   481 GGGCCTGCAC TTCCTCCAGG AGGACGCCCC CGACGAGATG GGCAAGTACN TNNAGAGCTT   541 CGTGGAGAGA GTGCTGAANA NNNNGCANTA NGCGGCCGCG ACTCNANNTN NTAATCAGCC   601 NTACNACATT TGTANAGGNT TTNCTTGCTT TAAAAANCCT CCCNCACCTC CCCCTGAACC   661 NGAANNNNAA ATGNNTGCAN NNGNTGNTNG NNNACTNNNT ATNGNNNNNT TANAANGGNN 721 TNNNNNNNNA NNNNNANCNN N //
Primer: SV40pro_ori_F (Pr0731) Region: SV 40pro_ori,NeoR_KanR Sequence file: RDB13338_A5E8h.seq
>13338_13338_B1G9F_3_SV40pro_ori_F_B02_05_ABI24.ab1     1 NNNNNNNNNN NNNNNGCCGC CTCNGCCTCT GANCTATTCC NGAAGTAGTG AGGAGGCTTT    61 TTTGGAGGCC TAGGCTTTTG CAAAGATCGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT   121 GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG   181 CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC   241 GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA   301 AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT   361 CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA   421 TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG   481 GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT   541 CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA   601 GCATCAGGGG CTCGCGCCNG CCGAACTGTT CGCCNGGCTC NAGGCNANCA TGCCCGACGG   661 CGANGNNCTC GNCGTGANCC NNGNNNANGN CNGCNNGNNN ANNNCNNNGN NNNAANNGNN 721 NNCNTTNCNN NNNNCNTCNN CNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.11.23

GNP_filter3_RDBDEP_html_231027.pl