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pCNL-N1_ITPKA (#RDB13348)

Cyan Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A.

Clone info. Expression vector of the Cyan Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A.
Comment Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. The ITPKA portion of this plasmid was derivedfrom a plasmid made by Dr. Michael John Schell, Johns Hopkins Medical Institutions. CNL contains a portion of mTurquoise2 (CFP mutant) provided by Dr. Joachim Goedhart, the University of Amsterdam.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli)
Gene/insert name rat inositol triphosphate 3-kinase A cDNA
Depositor|Developer Okada, Yasushi | Takai, Akira |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature(s) designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13348 pCNL-N1_ITPKA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCNL-N1_ITPKA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13348).

Reference section:

Takai, A., Nakano, M., Saito, K., Haruno, R., Watanabe, T.M., Ohyanagi, T., Jin, T., Okada, Y., Nagai, T., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13348_A5E8p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert 5'(rat ITPKA(partial),mTurquoise2 5')
Sequence file: RDB13348_A5E8g.seq check
>13348_13348_B1I6F_CMV-Forward_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNTNNN NNNNNATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
   61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGACTC AGATCTCGAG
  121 CTCAAGCTTA TGGGCATGGC GCGACCACGG GGCGCGGGGC CCTGCAGCCC CGGGTTGGAG
  181 CGGGCTCCGC GCCGGAGCGT CGGGGAGCTG CGCCTGCTCT TCGAAGCGCG CTGCGCCGCA
  241 GTCGCCGCCG CAGCGGCCGC AGGGTCGGAT CCACCGGTCG CCACCATGGT GAGCAAGGGC
  301 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
  361 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
  421 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
  481 TCCTGGGGCG TGCAGTGCTT CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
  541 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
  601 AACTACAAGA CCCGCGCCNA GGTGAANTTC GAGGGCGACA CCCTGGTGAA CCGCATCNAG
  661 CTGANNGNCA TCNACNTCNN NNNGANNGNA NATCCNGGGG NACNNGCNNG NAGTACNACN
721 ANTTNNNCNN NNNNNNNN
//
Primer: Rluc8-C_F2 (Pr1028)
Region: insert 3'(Rluc8 3'),SV40 pA,f1 ori
Sequence file: RDB13348_A5E8b.seq check
>13348_13348_B1I6F_3_Rluc8-C_F2_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTTCG NNNNNNNGAA GGGCCTGCAC TTCCTCCAGG AGGACGCCCC
   61 CGACGAGATG GGCAAGTACA TCAAGAGCTT CGTGGAGAGA GTGCTGAAGA ACGAGCAGTA
  121 GGCGGCCGCG ACTCTAGATC ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT
  181 TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG
  241 TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA
  301 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT
  361 CTTAAGGCGT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA
  421 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA
  481 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC
  541 GTGGACTCCN ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA
  601 CCATCACCCT AATCAAGTTT TTTGGGGNCG ANGTGNCGTA AAGCACTNAA TCGGANCCTA
  661 ANNNNNCCCC CNATTNNNAN NTNGNNNGGN AANCNNGNNA NNNNGNNANA ANGNNNGNAN
721 NAANNNNANG NANNNGGNNC NNNGNNNNNN NNNN
//
Primer: VN_F1 (Pr0992)
Region: insert mid(mTurquiose2 3',Rluc8 5')
Sequence file: RDB13348_A5E8c.seq check
>13348_13348_B1I6F_3_VN_F1_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNNNNCGTCT ATATNNCCGC CGACAAGCAG AAGAACGGCA
   61 TCAAGGCCAA CTTCAAGATC CGCCACAACA TCGAGGACGG CGGCGTGCAG CTCGCCGACC
  121 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
  181 TGAGCACCCA GTCCAAGCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC
  241 TGGAGTTCGT GACCGCCGCC GGGGGTACCA AGGTGTACGA CCCCGAGCAG AGGAAGAGGA
  301 TGATCACCGG CCCCCAGTGG TGGGCCAGGT GCAAGCAGAT GAACGTGCTG GACAGCTTCA
  361 TCAACTACTA CGACAGCGAG AAGCACGCCG AGAACGCCGT GATCTTCCTG CACGGCAACG
  421 CCACTAGCAG CTACCTGTGG AGGCACGTGG TGCCCCACAT CGAGCCCGTG GCCAGGTGCA
  481 TCATCCCCGA TCTGATCGGC ATGGGCAAGA GCGGNNNNAN CGGCAACGGC AGCTACAGGC
541 TGCTGGACCA CNNNNAGTAC CNNNNNNCCT GGTTNNNNNN NNNN
//
Primer: EGFP-C (Pr0025)
Region: insert mid(Rluc8)
Sequence file: RDB13348_A5E8d.seq check
>13348_13348_B1I6F_3_EGFP-C_C02_08_ABI24.ab1
    1 GNNNNNNNNN NNNNGANCCC NANCNGAGGA AGAGGANGAT CACCGGCCCC CAGTGGTGGG
   61 CCAGGTGCAA GCAGATGAAC GTGCTGGACA GCTTCATCAA CTACTACGAC AGCGAGAAGC
  121 ACGCCGAGAA CGCCGTGATC TTCCTGCACG GCAACGCCAC TAGCAGCTAC CTGTGGAGGC
  181 ACGTGGTGCC CCACATCGAG CCCGTGGCCA GGTGCATCAT CCCCGATCTG ATCGGCATGG
  241 GCAAGAGCGG CAAGAGCGGC AACGGCAGCT ACAGGCTGCT GGACCACTAC AAGTACCTGA
  301 CCGCCTGGTT CGAGCTTCTG AACCTGCCCA AGAAGATCAT CTTCGTGGGC CACGACTGGG
  361 GCGCCGCCCT GGCCTTCCAC TACGCCTACG AGCACCAGGA CAGGATCAAG GCCATCGTGC
  421 ACATGGAGAG CGTGGTGGAC GTGATCGAGA GCTGGGACGA GTGGCCAGAC ATCGAGGAGG
  481 ACATCGCCCT GATCAAGAGC GAGGAGGGCG AGAAGATGGT GCTGGAGAAC AACTTCTTCG
  541 TGGAGACCGT GCTGCCCAGC AAGATCATGA GAAAGCTGGA GCCCGAGGAN TTCGCCGCCT
  601 ACCTGGAGCC CTTCAAGGAN ANGGNCGANG TGAGAANACC CNCCCTGNNC TNGCCNNNAG
  661 AGATCCCCCN GNNGAANGNN NGCAGNCCGN NNNNNNGNNN ATCNNGNNNA ANNNNNNNNN
721 N
//
Primer: RLuc8-M_F (Pr0580)
Region: insert 3'(Rluc8 3')
Sequence file: RDB13348_A5E8e.seq check
>13348_13348_B1I6F_3_Rluc8-M_F_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN CGCCNACGAG CACCAGGACA GGATCAAGGC CATCGTGCAC
   61 ATGGAGAGCG TGGTGGACGT GATCGAGAGC TGGGACGAGT GGCCAGACAT CGAGGAGGAC
  121 ATCGCCCTGA TCAAGAGCGA GGAGGGCGAG AAGATGGTGC TGGAGAACAA CTTCTTCGTG
  181 GAGACCGTGC TGCCCAGCAA GATCATGAGA AAGCTGGAGC CCGAGGAGTT CGCCGCCTAC
  241 CTGGAGCCCT TCAAGGAGAA GGGCGAGGTG AGAAGACCCA CCCTGAGCTG GCCCAGAGAG
  301 ATCCCCCTGG TGAAGGGCGG CAAGCCCGAC GTGGTGCAGA TCGTGAGAAA CTACAACGCC
  361 TACCTGAGAG CCAGCGACGA CCTGCCCAAG CTGTTCATCG AGGGCGACCC CGGCTTCTTC
  421 AGCAACGCCA TCGTGGAGGG CGCCAAGAAG TTCCCCAACA CCGAGTTCGT GAAGGTGAAG
  481 GGCCTGCACT TCCTCCAGGA GGACGCCCCC GACGAGATGG GCAAGTACAT CAAGAGCTTC
  541 GTGGAGAGAG TGCTGAAGAA CGAGCAGTAG GCGGCCGCGA CTCTAGATCA TAATCAGCCA
  601 TACCACATTT GTAGAGGTTT TACTTGCTTT NAAAAACCTC CCACNCCTCC CCCTGAACCT
  661 GNNNNTAAAA NGANNNNNAN NGTNGNTGNT NACTNGNTNA TNGNNNCNTN NNNGGNNANA
721 ANTAAAGCAN ANNNTCNCNA NNTTNNNNNA NNAANNNNNT TTTNNNNNNN NNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori,NeoR_KanR
Sequence file: RDB13348_A5E8f.seq check
>13348_13348_B1I6F_3_SV40pro_ori_F_E02_14_ABI24.ab1
    1 NNNNNNNGNN GNNGCCGCCT CNGCCTCTGA NCTATTCNNG AAGTAGTGAG GAGGCTTTTT
   61 TGGAGGCCTA GGCTTTTGCA AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA
  121 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT
  181 ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC
  241 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG
  301 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG
  361 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC
  421 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC
  481 GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG
  541 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC
  601 ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAN GGCGAGCATG CCCGACNGCN
  661 AGGNTCTCGT CNTGNNCCAN GGCNANGCCN GNTTGNCGAA NANCNTGGNG GNAAANGNNN
721 NNTTTNNNGN NTCNNCNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]
reference Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043. [link to RRC of NBRP]