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pONL-N1_ITPKA (#RDB13349)

Orange Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A.

Clone info. Expression vector of the Orange Nano-lantern for labeling of F-actin by the rat inositol triphosphate 3-kinase A.
Comment Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. The ITPKA portion of this plasmid was derivedfrom a plasmid made by Dr. Michael John Schell, Johns Hopkins Medical Institutions. ONL contains a portion of hmKusabiraOrange2 provided by Dr. Atsushi Miyawaki, the RIKEN BSI.
Vector backbone pEGFP-N1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli)
Growth conditions LB + Kan, 37C
Gene/insert name rat inositol triphosphate 3-kinase A cDNA
Depositor|Developer Okada, Yasushi | Takai, Akira |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS.
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 本件リソースの利用は、非営利機関による学術研究に限る。利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。利用者は、mKO2について理化学研究所CBS 宮脇チームから、書面による提供承諾を得ること。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13349 pONL-N1_ITPKA DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pONL-N1_ITPKA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13349).

Reference section:

Takai, A., Nakano, M., Saito, K., Haruno, R., Watanabe, T.M., Ohyanagi, T., Jin, T., Okada, Y., Nagai, T., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13349_A5E8p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert 5'(r ITPKA,hmKO)
Sequence file: RDB13349_A5E8a.seq check
>13349_13349_A5E8_3_CMV_Forward_D02_11.ab1
    1 NNNNNNNNNN NCNNTTGNNG CNNTGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTCGAGCTC
  121 AAGCTTATGG GCATGGCGCG ACCACGGGGC GCGGGGCCCT GCAGCCCCGG GTTGGAGCGG
  181 GCTCCGCGCC GGAGCGTCGG GGAGCTGCGC CTGCTCTTCG AAGCGCGCTG CGCCGCAGTC
  241 GCCGCCGCAG CGGCCGCAGG GTCGGATCCA CCGGTCGCCA CCATGGTGAG CGTGATCAAG
  301 CCCGAGATGA AGATGAGGTA CTACATGGAC GGCTCCGTCA ATGGGCATGA GTTCACAATC
  361 GAGGGTGAGG GCACAGGCAG ACCTTACGAG GGACATCAGG AGATGACACT GCGCGTCACA
  421 ATGGCCGAGG GCGGGCCAAT GCCTTTCGCC TTCGACCTGG TGTCCCACGT GTTCTGTTAC
  481 GGCCACAGAG TTTTTACTAA ATATCCAGAA NAGATCCCAN ACTATTTCAA GCAGGCCTTT
541 CCTGAGGNNN NNNNNNN
//
Primer: Rluc8-M_F2 (Pr1033)
Region: insert 3'(Rluc8.6-535)
Sequence file: RDB13349_A5E8b.seq check
>13349_13349_-B1G9F_Rluc8-M_F2_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGG GGNNCGCTCT GGCCTTTCNC TACGCCTACG AGCACCAAGA
   61 CAGGATCAAG GCCATCGTCC ATATGGAGAG TGTCGTGGAC GTGATCGAGT CCTGGATGGG
  121 GTGGCCTGAC ATCGAGGAGG AGCTGGCCCT GATCAAGAGC GAAGAGGGCG AGAAAATGGT
  181 GCTTGAGAAT AACTTCTTCG TCGAGACCCT GTTGCCAAGC AAGATCATGC GGAAACTGGA
  241 GCCTGAGGAG TTCGCTGCCT ACCTGGAGCC ATTCAAGGAG AAGGGCGAGG TTAGACGGCC
  301 TACCCTCTCC TGGCCTCGCG AGATCCCTCT CGTTAAGGGA GGCAAGCCCG ACGTCGTCCA
  361 GATTGTCCGC AACTACAACG CCTACCTTCG GGCCAGCGAC GATCTGCCTA AGCTGTTCAT
  421 CGAGTCCGAC CCTGGGTTCT TTTCCAACGC TATTGTCGAG GGAGCTAAGA AGTTCCCTAA
  481 CACCGAGTTC GTGAAGGTGA AGGGCCTCCA CTTCCTCCAG GAGGACGCTC CAGATGAAAT
  541 GGGTAAGTAC ATCAAGAGCT TCGTGGAGCG CGTGCTGAAG AACGAGCAGT AGGCGGCCGC
  601 GACTCTAGAT CATAATCNGC CATACCACAT TTGTAGAGGT TTTACTTGCT TTAAAAAACC
  661 TCCCNCNCNT CCCCCNGNNN CNGANNATAA AATGNATGNN ATTGNTGNTG NNNNCTNGNT
721 NATNNNNNCT NNNNNNNGNN NNNNNNNN
//
Primer: hRluc_N_rev (Pr0567)
Region: insert mid(Rluc8.6-535,hmKO)
Sequence file: RDB13349_A5E8c.seq check
>13349_13349_B1GfF_3_hRluc_N_rev_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNTTNGCT TGNNGCGAGC CCNCCACTGA GGCCCAGTGA TCATGCGTTT
   61 GCGTTGCTCG GGGTCGTACA CCTTGGTACC GGAGTGGGCC ACGGCGTCCT CCACCTGCTC
  121 GGTGATGTTG CCCTCGGTCT TCCTCACCAG CCTGTGGCCG ATGTAGTGGT CGCCGGGCAT
  181 CTCCAGGATC TCCTTGGCGG CCTTGTAGGT GGTCTTCATC TGGCACTTGT GGTTGCCGCC
  241 GCCCTCCAGC TTCAGGTACA TGGTCACGTC GCCCTTCAGC ACGCCGTCGC TGGCGGTGAT
  301 CTTCTCGGTG GAGGGCTCCC AGTCCACGCT CTGGTTCTGC ATGATGGGGC CGTCGGCGGG
  361 GAAGTTCACG CCGGTGAACT TGGACTTGTG GTAGAAGGTG TTGCCCCTCA GGCTGATGTG
  421 GGCGCTCACG GAGGCGGAGC CGCCGTCCTC GAACTCCAGG GACCTCTCCC AGGACAGGCC
  481 CTCAGGAAAG GCCTGCTTGA AATAGTCTGG GATCTCTTCT GGATATTTAG TAAAAACTCT
  541 GTGGCCGTAA CAGAACACGT GGGANACCAG GTCGAANGCG AAAGGCATTG GCCCGCCCTC
  601 NGCCNTTGTG ACGCGCAGTG TCNTCTCCTG ATGTNCCTCN TAANGNCNGN CTGTGCCNTC
  661 NCCCTCNANT GNGAACNCNT GCCCATTGAN NNNNNTCNNT GNNNNANNTN NTCNTNNTCT
721 NNGGCNNGNT NNCNCTNNNN NNGNNNNNNN NNN
//
Primer: mKO2-M_F (Pr0578)
Region: insert mid(hmKO,Rluc8.6-535)
Sequence file: RDB13349_A5E8d.seq check
>13349_13349_B1GfF_3_mKO2-M_F_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNGCNNGANG AAGANCACCT ACNAGGCCGC CAAGGAGATC
   61 CTGGAGATGC CCGGCGACCA CTACATCGGC CACAGGCTGG TGAGGAAGAC CGAGGGCAAC
  121 ATCACCGAGC AGGTGGAGGA CGCCGTGGCC CACTCCGGTA CCAAGGTGTA CGACCCCGAG
  181 CAACGCAAAC GCATGATCAC TGGGCCTCAG TGGTGGGCTC GCTGCAAGCA AATGAACGTG
  241 CTGGACTCCT TCATCAACTA CTATGATTCC GAGAAGCACG CCGAGAACGC CGTGATTTTT
  301 CTGCATGGTA ACGCTACCTC CAGCTACCTG TGGAGGCACG TCGTGCCTCA CATCGAGCCC
  361 GTGGCTAGAT GCATCATCCC TGATCTGATC GGAATGGGTA AGTCCGGCAA GAGCGGGAAT
  421 GGCTCATATC GCCTCCTGGA TCACTACAAG TACCTCACCG CTTGGTTCGA GCTGCTGAAC
  481 CTTCCAAAGA AAATCATCTT TGTGGGCCAC GACTGGGGGA GCGCTCTGGC CTTTCACTAC
  541 GCCTACGAGC ACCAAGACAG GATCAAGGCC ATCGTCCATA TGGAGAGTGT CGTGGANGTG
  601 ATCGANTCCT GNATGGGGTG GCCTGACNTC GANGANNANC TGGNCCNGAT CAAGAGCGAA
661 NANGGNGANA AAATGGTGCT TGANAANNAC TNCNNCNNCN ANANCNNNNN NNNNN
//
Primer: RLuc8-m1dN3-C_F (Pr0265)
Region: insert 3'(Rluc8.6-535),SV40 pA,f1 ori
Sequence file: RDB13349_A5E8e.seq check
>13349_13349_B1GfF_3_Rluc8-m1dN3-c_F_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCTT CGTGGANCGC GTGCTGAAGA ACGAGCAGTA GGCGGCCGCG
   61 ACTCTAGATC ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT TAAAAAACCT
  121 CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG TTAACTTGTT
  181 TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC
  241 ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTAAGGCGT
  301 AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA ATCAGCTCAT
  361 TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA TAGACCGAGA
  421 TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC GTGGACTCCA
  481 ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA CCATCACCCT
  541 AATCAAGTTT TTTGGGGTCG AGGTGNCGTA AAGCACNAAA TCGGAACCCT AAAGGGAGCC
  601 CCCGATTTAN AGCTTGACGG NGAAAGCCNG CGANNGTGNC GANAAANGAA NGGAANAAAG
  661 NNNAAGGANC GGGCGCTNNG GCNCNGGCNA GNGNANCGGT CANNCTGCNC NNANCNNCCN
721 NNCCNNNNNN NNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40pro_ori,NeoR_KanR
Sequence file: RDB13349_A5E8f.seq check
>13349_13349_B1G9F_SV40pro_ori_F_H01_22_ABI24.ab1
    1 NNNNNNNNNN NNGCCGCCTC NGCCTCTGAN CTATTCCNGA AGTAGTGAGG AGGCTTTTTT
   61 GGAGGCCTAG GCTTTTGCAA AGATCGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT
  121 TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA
  181 TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA
  241 GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAAGA
  301 CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA
  361 CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT
  421 CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG
  481 GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA
  541 GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA
  601 TCNNNNNCTC GCGCCNGCCG AACTGTTCGC NNGGCTCNNN GCGANCATNN NNNANGGCGA
  661 GGNNCTCGTC NTGNNCCANG NNNATNCNTG CTTNNCGAAN ATCATGGNNG AAANNGGNNN
721 CNTTNNNNNN NNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [link to RRC of NBRP]
reference Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043. [link to RRC of NBRP]