RDB13355 - pONL-N1

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pONL-N1_Golgi (#RDB13355)

Orange Nano-lantern for labeling of Golgi apparatus by the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase.

Clone info.	Expression vector of the Orange Nano-lantern for labeling of Golgi apparatus by the N-terminal 81 amino acids of human beta 1,4-galactosyltransferase.
Comment	Expression was confirmed by the depositor with transfection into MDCKII cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Although this clone is constructed on the pEGFP, it doesn't contain the original GFP. ONL contains a portion of hmKusabiraOrange2 provided by Dr. Atsushi Miyawaki, the RIKEN BSI.
Vector backbone	pEGFP-N1 (Plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^r (E. coli)
Growth conditions	LB + Kan, 37C
Gene/insert name	human beta 1,4-galactosyltransferase cDNA
Depositor\|Developer	Okada, Yasushi \| Takai, Akira \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015).
Additional terms and conditions:
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). Additional terms and conditions: When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	本件リソースの利用は、非営利機関による学術研究に限る。利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。付加的使用条件： When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested. Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

MTAに書く使用条件

本件リソースの利用は、非営利機関による学術研究に限る。利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。
付加的使用条件：
When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Atsushi Miyawaki or an acknowledgement to Dr. Miyawaki is requested.
Karasawa, S. et al., Biochem. J. 381: 307-312 (2004).

Catalog #	Resource name	Shipping form	Fee
RDB13355	pONL-N1_Golgi	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pONL-N1_Golgi was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13355).

Reference section:

Takai, A., Nakano, M., Saito, K., Haruno, R., Watanabe, T.M., Ohyanagi, T., Jin, T., Okada, Y., Nagai, T., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	Data Sheet open

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB13355_A5E8a.seq
>13355_13355_A5E8_3_CMV_Forward_H10_22.ab1 1 AGTTCACTCC GCCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCATG AGGCTTCGGG AGCCGCTCCT 121 GAGCGGCAGC GCCGCGATGC CAGGCGCGTC CCTACAGCGG GCCTGCCGCC TGCTCGTGGC 181 CGTCTGCGCT CTGCACCTTG GCGTCACCCT CGTTTACTAC CTGGCTGGCC GCGACCTGAG 241 CCGCCTGCCC CAACTGGTCG GAGTCTCCAC ACCGCTGCAG GGCGGCTCGA ACAGTGCCGC 301 CGCCATCGGG CAGTCCTCCG GGGAGCTCCG GACCGGAGGG GCCAAGGATC CACCGGTCGC 361 CACCATGGTG AGCGTGATCA AGCCCGAGAT GAAGATGAGG TACTACATGG ACGGCTCCGT 421 CAATGGGCAT GAGTTCACAA TCGAGGGTGA GGGCACAGGC AGACCTTACG AGGGACATCA 481 GGAGATGACA CTGCGCGTCA CAATGGCCGA GGGCGGGCCA ATGCCTTTCG CCTTCGACCT 541 GGTGTCCCAC GTGTTCTGTT ACGGCCACAG AGTTTTTACT AAATATCCAG AAGAGATCCC 601 AGACTATTTC AAGCAGGCCT TTCCTGAGGG CCTGTCCTGG GAGAGGTCCC TGGAGTTCGA 661 GGACGGCGGC TCCGCCTCCG TGAGCGCCCA CATCAGCCTG AGGGGCAACA CCTTCTACCA 721 CAAGTCCAAG TTCACCGGCG TGAACTTCCC CGCCGACGGC CCCATCATGC AGAACCAGAG 781 CGTGGACTGG GAGCCCTCCA CCGAGAAGAT CACCGCCAGC GACGGCGTGC TGAAAGGGCG 841 ACGTGACCAT GTACCTGAAG CTGGAGGGCG GCGGGCAACC CACAAGTGCC AGATGAAGAC 901 CACCCTACAA GGCCGCCCAA GGAGATCCCT GGAGATGCCC GGGCGACCAC TACCATCGGG 961 CCACAGCTGG TGAGAAGACC GGAAGGCCAC CATCACCGGA GCAGTGAAGG AACGCGTTGC 1021 CCACCTCCGT ACCAAGTGTA CGAACCCCGA GCATCGCAAC GCATGATCAC TGAGCCCTCA 1081 TTTGGAATG //
Primer: ME-1290Rv Sequence file: RDB13355_A5E8b.seq
>13355_13355_A5E8_3_ME_1290Rv_H06_24.ab1 1 ATGCCATTCA TTTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG 61 TAAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCTAG AGTCGCGGCC GCCTACTGCT 121 CGTTCTTCAG CACGCGCTCC ACGAAGCTCT TGATGTACTT ACCCATTTCA TCTGGAGCGT 181 CCTCCTGGAG GAAGTGGAGG CCCTTCACCT TCACGAACTC GGTGTTAGGG AACTTCTTAG 241 CTCCCTCGAC AATAGCGTTG GAAAAGAACC CAGGGTCGGA CTCGATGAAC AGCTTAGGCA 301 GATCGTCGCT GGCCCGAAGG TAGGCGTTGT AGTTGCGGAC AATCTGGACG ACGTCGGGCT 361 TGCCTCCCTT AACGAGAGGG ATCTCGCGAG GCCAGGAGAG GGTAGGCCGT CTAACCTCGC 421 CCTTCTCCTT GAATGGCTCC AGGTAGGCAG CGAACTCCTC AGGCTCCAGT TTCCGCATGA 481 TCTTGCTTGG CAACAGGGTC TCGACGAAGA AGTTATTCTC AAGCACCATT TTCTCGCCCT 541 CTTCGCTCTT GATCAGGGCC AGCTCCTCCT CGATGTCAGG CCACCCCATC CAGGACTCGA 601 TCACGTCCAC GACACTCTCC ATATGGACGA TGGCCTTGAT CCTGTCTTGG TGCTCGTAGG 661 CGTAGTGAAA GGCCAGAGCG CTCCCCCAGT CGTGGCCCAC AAAGATGATT TTCTTTGGAA 721 GGTTCAGCAG CTCGAACCAA GCGGTGAGGT ACTTGTAGTG ATCCAGGAGG CGATATGAGC 781 CATTCCCGCT CTTGCCGGAC TTACCCATTC CGATCAGATC AGGGATGATG CATCTAGCCA 841 CGGGCTCGAT GTGAGGCACG ACGTGCCTCC ACAGGTAGCT GGAGGTAGCG TTACCATGCA 901 GAAAAATCAC GGCGTTCTCG GCGTGCTTCT CGGAATCATA GTAGTTGATG AAGGAGTCCA 961 GCACGTTCAT TTGCTTGCAG CGAGCCCACC ACTGAGGCCC AGTGATCATG CGTTTGCGTT 1021 GCTCGGGGTC GTACACCTTT GGTACGGAGT GGGCCACGGC GTCTCACTGC TCGATGATGG 1081 TGCCTCGGTC TCCTTCAACA GGCTGATGGA ACCGAGTGTT AGGTTGGCAT TC //
Primer: SV40pro_F_V2 Sequence file: RDB13355_A5E8c.seq
>13355_13355_A5E8_SV40pro_F_V2_C03_03_ABI08.ab1 1 CTACGACCCT TTTTTTGGAG GCCTAGGCTT TTGCAAGATC GATCAAGAGA CAGGATGAGG 61 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA 121 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT 181 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT 241 GAATGAACTG CAAGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG 301 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT 361 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC 421 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC 481 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA 541 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGAG 601 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT 661 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG 721 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC 781 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA 841 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC CGACCAAGCG 901 ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG GCCTTCTATG AAAGGTTGGG 961 GCTTCGGAAT CGTTTTTCCG GGACGCCGGC TGGGATGAAT CCTCCAGCGC GGGGAATCTC 1021 ATGCTGGGAG TTCTTCGCCC AACCCTTAGG GGGAGGCTAC TGAAACACCG GAAGGGAGAA 1081 CAAATACCGG AAGAATCCCG CTGCTATGAA CGGCCAATAA AAGACCGATA ACGCACACGG 1141 GGTGTTGGGG TCCGATTTTG GTTTCTCAAT A //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507. [PubMed] [Article] [RRC of NBRP]
reference	Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043. [PubMed] [Article] [RRC of NBRP]

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human beta 1,4-galactosyltransferase

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human beta 1,4-galactosyltransferase

Reference sequence