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pGF-mSox2-ONL

Lentiviral reporter plasmid of mouse Sox2 by the Orange Nano-lantern.

Catalog number RDB13379
Resource name pGF-mSox2-ONL
Clone info. Lentiviral reporter plasmid of mouse Sox2 by the Orange Nano-lantern.
Comment Expression was confirmed by the depositor with transfection into murine ES cells. Please refer Takai, A. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015, (PMID 25831507) for the conditions of luminescence imaging and substrate. Reporter gene was deleted from the original vector. ONL contains a portion of hmKusabiraOrange2 provided by Dr. Atsushi Miyawaki, the RIKEN BSI.
Vector backbone pGreenFire1 (Plasmid)
Size of vector backbone 9.4 kb
Gene/insert name mouse Sox2 genomic DNA
Depositor|Developer Okada, Yasushi | Takai, Akira |
Other clones in our bank

External Database
mouse Sox2

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of literature designated by the DEPOSITOR are requested (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356, 2015). RECIPIENT is requested to obtain written consent of Dr. Miyawaki team of the RIKEN CBS.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Approval form [Word] by Dr. Atsushi Miyawaki
          Remarks Remember that you will be working with samples containing infectious virus.
          Please obtain written approval of Dr. Atsushi Miyawaki.
          提供案内 (日本国内) [open/close]

          提供条件 本件リソースの利用は、非営利機関による学術研究に限る。利用者は、研究成果の公表にあたって謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Takai, A. et al., Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015))。利用者は、mKO2について理化学研究所CBS 宮脇チームから、書面による提供承諾を得ること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          宮脇敦史博士による提供承諾書 [Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
          宮脇敦史博士の書面による提供承諾が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB13379 pGF-mSox2-ONL DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Nano-lantern (English text)
          Featured content Nano-lantern (Japanese text)
          Featured content Reguration of genes in ES cells (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13379_A5E8p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: env_F
          Sequence file: RDB13379_A5E8a.seq check
          >13379_13379_A5E8_3_env_F_E09_15.ab1
              1 GGCGTGCAGG ATCCATTCGA TTAGTGACGG ATCTGCGACG GTATCGGTTA ACTTTTAAAA
             61 GAAAAGGGGG GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG
            121 ACATACAAAC TAAAGAATTA CAAAAACAAA TTACAAAATT CAAAATTTTA TCGATGAATT
            181 CTAATTAATG CAGAGACTCT AAAAGAATTT CCCGGGCTCG GGCAGCCATT GTGATGCATA
            241 TAGGATTATT CACGTGGTAA TGTAATTAAT GCAGAGACTC TAAAAGAATT TCCCGGGCTC
            301 GGGCAGCCAT TGTGATGCAT ATAGGATTAT TCACGTGGTA ATGCCAGTCC AAGCTAGGCA
            361 GGTTCCCCTC TAATTAATGC AGAGACTCTA AAAGAATTTC CCGGGCTCGG GCAGCCATTG
            421 TGATGCATAT AGGATTATTC ACGTGGTAAT GAGCACAGTC GACAGTTCTT GCGGATCCAC
            481 CATGGTGAGC GTGATCAAGC CCGAGATGAA GATGAGGTAC TACATGGACG GCTCCGTCAA
            541 TGGGCATGAG TTCACAATCG AGGGTGAGGG CACAGGCAGA CCTTACGAGG GACATCAGGA
            601 GATGACACTG CGCGTCACAA TGGCCGAGGG CGGGCCAATG CCTTTCGCCT TCGACCTGGT
            661 GTCCCACGTG TTCTGTTACG GCCACAGAGT TTTTACTAAA TATCCAGAAG AGATCCCAGA
            721 CTATTTCAAG CAGGCCTTTC CTGAGGGCCT GTCCTGGGAG AGGTCCCTGG AGTTCGAGGA
            781 CGGCGGCTCC GCCTCCGTGA GCGCCCACAT CAGCCTGAGG GGCAACACCT TCTACCACAA
            841 GTCCAAGTTC ACCGGCGTGA ACTTCCCCGC CGACGGCCCC ATCATGCAGA ACCAGAGCGT
            901 GGACTGGGAG CCCTCCACCG AGAAGATCAC CGCCAGCGAC GGCGTGCTGA AAGGGCGACG
            961 TGACCATGTA CCTGAAGCTG GAGGGCGGCG GCAACCACAA GTGCCAGATG AAGACCACCC
           1021 TACACGGCCG CCCAAGGAGA TCCCTGGAGA TGCCCGGCGA CCACTACATC GGGCCACAGG
           1081 CTGCTTGAGA AGACCGAGGG GCAACATTCA CGAGCAAGTG AAGAACGGCC GTGGACCACC
          1141 TCCGGTACCA AGGGTGGTTA ACCGAA
          //
          Primer: ONL-M_R
          Sequence file: RDB13379_A5E8b.seq check
          >13379_13379_A9D8F_3_ONL-M_R_A08_02_ABI24.ab1
              1 CTTTAGTTGC CTCGGTCTTC TCACCAGCCT GTGGCCGATG TAGTGGTCGC CGGGCATCTC
             61 CAGGATCTCC TTGGCGGCCT TGTAGGTGGT CTTCATCTGG CACTTGTGGT TGCCGCCGCC
            121 CTCCAGCTTC AGGTACATGG TCACGTCGCC CTTCAGCACG CCGTCGCTGG CGGTGATCTT
            181 CTCGGTGGAG GGCTCCCAGT CCACGCTCTG GTTCTGCATG ATGGGGCCGT CGGCGGGGAA
            241 GTTCACGCCG GTGAACTTGG ACTTGTGGTA GAAGGTGTTG CCCCTCAGGC TGATGTGGGC
            301 GCTCACGGAG GCGGAGCCGC CGTCCTCGAA CTCCAGGGAC CTCTCCCAGG ACAGGCCCTC
            361 AGGAAAGGCC TGCTTGAAAT AGTCTGGGAT CTCTTCTGGA TATTTAGTAA AAACTCTGTG
            421 GCCGTAACAG AACACGTGGG ACACCAGGTC GAAGGCGAAA GGCATTGGCC CGCCCTCGGC
            481 CATTGTGACG CGCAGTGTCA TCTCCTGATG TCCCTCGTAA GGTCTGCCTG TGCCCTCACC
            541 CTCGATTGTG AACTCATGCC CATTGACGGA GCCGTCCATG TAGTACCTCA TCTTCATCTC
            601 GGGCTTGATC ACGCTCACCA TGGTGGATCC GCAAGAACTG TCGACTGTGC TCATTACCAC
            661 GTGAATAATC CTATATGCAT CACAATGGCT GCCCGAGCCC GGGAAATTCT TTTAGAGTCT
            721 CTGCATTAAT TAGAGGGGAA CCTGCCTAGC TTGGACTGGC ATTACCACGT GAATAATCCT
            781 ATATGCATCA CAATGGCTGC CCGAGCCCGG GAAATTCTTT TAGAGTCTCT GCATTAATTA
            841 CATTACCACG TGAATAATCC TATATGCAAT CACAATGGCT GCCCGAGCCC GGGAAATTTC
            901 TTTTAGAGTC TCTGCATTAA TTAGAATCAT CGATAAAATT TTTGAATTTT GTAATTTGGT
            961 TTTTGGAAAT TCTTTAGTTT GTATGTCTGG TTGCTATTAT GTCTACTATT CTTTCCCCTG
           1021 CACTGTACCC CCCAATCCCC CCCTTTTCTT TAAAAGTTAA ACCGAATACC GGTCCAAGAA
           1081 TCCGGTTTCA CTAAGTCGGA ATGGAATTCT AGATCTCTAT CTCATCATCT TCTCAACGAT
          1141 CCTGTATCTA TGTCGAATTA TCCCAGTGCC GGGGCCCGTA TGATTG
          //
          Primer: mKO2-M_F
          Sequence file: RDB13379_A5E8c.seq check
          >13379_13379_A9D8F_3_mKO2-M_F_C04_03_ABI08.ab1
              1 TGGGCGGGCG GCCAACAACA AGGTGGCCAG ATGAAGACCA CCTACAAGGC CGCCAAGGAG
             61 ATCCTGGAGA TGCCCGGCGA CCACTACATC GGCCACAGGC TGGTGAGGAA GACCGAGGGC
            121 AACATCACCG AGCAGGTGGA GGACGCCGTG GCCCACTCCG GTACCAAGGT GTACGACCCC
            181 GAGCAACGCA AACGCATGAT CACTGGGCCT CAGTGGTGGG CTCGCTGCAA GCAAATGAAC
            241 GTGCTGGACT CCTTCATCAA CTACTATGAT TCCGAGAAGC ACGCCGAGAA CGCCGTGATT
            301 TTTCTGCATG GTAACGCTAC CTCCAGCTAC CTGTGGAGGC ACGTCGTGCC TCACATCGAG
            361 CCCGTGGCTA GATGCATCAT CCCTGATCTG ATCGGAATGG GTAAGTCCGG CAAGAGCGGG
            421 AATGGCTCAT ATCGCCTCCT GGATCACTAC AAGTACCTCA CCGCTTGGTT CGAGCTGCTG
            481 AACCTTCCAA AGAAAATCAT CTTTGTGGGC CACGACTGGG GGAGCGCTCT GGCCTTTCAC
            541 TACGCCTACG AGCACCAAGA CAGGATCAAG GCCATCGTCC ATATGGAGAG TGTCGTGGAC
            601 GTGATCGAGT CCTGGATGGG GTGGCCTGAC ATCGAGGAGG AGCTGGCCCT GATCAAGAGC
            661 GAAGAGGGCG AGAAAATGGT GCTTGAGAAT AACTTCTTCG TCGAGACCCT GTTGCCAAGC
            721 AAGATCATGC GGGAAAACTG GAGCCTGAGG AGTTCGCTGC CTACCTGGAG CCATTCAAGG
            781 AAGAAGGGCG AGGTTAGACG GCCTACCCTC TCCTGGCCTC GCGAGATCCC CTCTCTTAAG
            841 GGAAGGCAAA GCCCGAACGT CGTCCAGATT GTTCCGCAAC TAACAACGGC CTACCTTCCG
            901 GCCAACCAAC GATCTTGCCT AAACTGTTTC ATCAAGTCCA ACCCCTGGGT TTCTTTTCCA
            961 CGCTTATTTG TCGAGGGGAG CTAAAAAAGT CCCTAGAGCC GAAGTTTTGT GAAGGTTGAA
           1021 CGGGGCCCCA ACTTCCTACC AGAAGGACGC TCGCAGAATA TAAATGGGGA AAGTACCACT
          1081 CAG
          //
          Primer: RLuc8.6-M_F
          Sequence file: RDB13379_A5E8d.seq check
          >13379_13379_A9D8F_3_RLuc8.6-M_F_H05_08_ABI08.ab1
              1 CGTAGTGGGG GGGGGCCTGA CATCGAGGAG GAGCTGGCCC TGATCAAGAG CGAAGAGGGC
             61 GAGAAAATGG TGCTTGAGAA TAACTTCTTC GTCGAGACCC TGTTGCCAAG CAAGATCATG
            121 CGGAAACTGG AGCCTGAGGA GTTCGCTGCC TACCTGGAGC CATTCAAGGA GAAGGGCGAG
            181 GTTAGACGGC CTACCCTCTC CTGGCCTCGC GAGATCCCTC TCGTTAAGGG AGGCAAGCCC
            241 GACGTCGTCC AGATTGTCCG CAACTACAAC GCCTACCTTC GGGCCAGCGA CGATCTGCCT
            301 AAGCTGTTCA TCGAGTCCGA CCCTGGGTTC TTTTCCAACG CTATTGTCGA GGGAGCTAAG
            361 AAGTTCCCTA ACACCGAGTT CGTGAAGGTG AAGGGCCTCC ACTTCCTCCA GGAGGACGCT
            421 CCAGATGAAA TGGGTAAGTA CATCAAGAGC TTCGTGGAGC GCGTGCTGAA GAACGAGCAG
          481 TAACTCGACT TTAAGACCAA TGACTTACAA GGCAGCTGTA GATC
          //
          Primer: pUC-M13_-100
          Sequence file: RDB13379_A5E8e.seq check
          >13379_13379_A9D8F_3_pUC-M13_-100_C08_08_ABI24.ab1
              1 GCCGTCAGGC GATTAGTTGG GTACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC
             61 GGCCAGTGCC AAGCTGACGC GTGTAGTCTT ATGCAATACT CTTGTAGTCT TGCAACATGG
            121 TAACGATGAG TTAGCAACAT GCCTTACAAG GAGAGAAAAA GCACCGTGCA TGCCGATTGG
            181 TGGAAGTAAG GTGGTACGAT CGTGCCTTAT TAGGAAGGCA ACAGACGGGT CTGACATGGA
            241 TTGGACGAAC CACTGAATTG CCGCATTGCA GAGATATTGT ATTTAAGTGC CTAGCTCGAT
            301 ACATAAACGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG
            361 GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC
            421 GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA
            481 TCTCTAGCAG TGGCGCCCGA ACAGGGACTT GAAAGCGAAA GGGAAACCAG AGGAGCTCTC
            541 TCGACGCAGG ACTCGGCTTG CTGAAGCGCG CACGGCAAGA GGCGAGGGGC GGCGACTGGT
            601 GAGTACGCCA AAAATTTTGA CTAGCGGAGG CTAGAAGGAG AGAGATGGGT GCGAGAGCGT
            661 CAGTATTAAG CGGGGGAGAA TTAGATCGCG ATGGGAAAAA ATTCGGTTAA GGCCAGGGGG
            721 AAAGAAAAAA TATAAATTAA AACATATAGT ATGGGCAAGC AGGGAGCTAG AACGATTCGC
            781 AGTTAATCCT GGCCTGTTAG AAACATCAGA AGCTGTAGAC AAATACTGGG ACAGCTACAA
            841 CCATCCCTTC AGACAGGATC AGAAGAACTT AGATCATTAT ATAATACAGT AGCAACCCTC
            901 TATTGTGTGC ATCAAAGGAT AGAGATAAAA GACACCAAGG AAGCTTTAGA CAAGATAGAG
            961 GAAGAGCAAA ACAAAAGTAA GACCACCGCA CAGCAAGCGG GCCGCTGATC TTCAGACCCT
           1021 GGGAGAAGGA GATATGGAGG GAACATTTGG GAGAAAGTGA ATTATATAAA TATAAGGTAG
           1081 TGAAAATTTG AAACCATAGG GAGTAGCACC CCAGCCAGGC AAAGAGAAGA GTTGTGCAGA
          1141 AAGCAAAAAG AGCATGGGGA TTAGGGAGCT TTTGTTTTCG CTCGTG
          //
          Primer: HIVgp_R1
          Sequence file: RDB13379_A5E8f.seq check
          >13379_13379_A9D8F_3_HIVgp_R1_D08_11_ABI24.ab1
              1 CCGGCCTCCT ATTCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG
             61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA
            121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGCTTGCT GTGCGGTGGT
            181 CTTACTTTTG TTTTGCTCTT CCTCTATCTT GTCTAAAGCT TCCTTGGTGT CTTTTATCTC
            241 TATCCTTTGA TGCACACAAT AGAGGGTTGC TACTGTATTA TATAATGATC TAAGTTCTTC
            301 TGATCCTGTC TGAAGGGATG GTTGTAGCTG TCCCAGTATT TGTCTACAGC CTTCTGATGT
            361 TTCTAACAGG CCAGGATTAA CTGCGAATCG TTCTAGCTCC CTGCTTGCCC ATACTATATG
            421 TTTTAATTTA TATTTTTTCT TTCCCCCTGG CCTTAACCGA ATTTTTTCCC ATCGCGATCT
            481 AATTCTCCCC CGCTTAATAC TGACGCTCTC GCACCCATCT CTCTCCTTCT AGCCTCCGCT
            541 AGTCAAAATT TTTGGCGTAC TCACCAGTCG CCGCCCCTCG CCTCTTGCCG TGCGCGCTTC
            601 AGCAAGCCGA GTCCTGCGTC GAGAGAGCTC CTCTGGTTTC CCTTTCGCTT TCAAGTCCCT
            661 GTTCGGGCGC CACTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG
            721 TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG CAAGCTTTAT
            781 TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT
            841 AACCAGAGAG ACCCGTTTAT GTATCGAGCT AGGCACTTAA ATACAATATC TCTGCAATGC
            901 GGCAATTCAG TGGTTCGTCC AATCCATGTC AGACCCGTCT GTTGCCTTCC TAATAAAGCA
            961 CGATCGTACC ACCTTACTTC CACCAATCGG CATGCACGGT GCTTTTTTCT CTCCTTGTAA
           1021 GGCATGTTGC TAACTCATCG TTACCATGTT TGCAGACTAC AGAGTATGCA TAAAGACTAC
           1081 ACGCGGTCAA GCTTGGCAAC TGGGCCGTTC GTTTACCACG TTCATGAACT GGAAACCTGG
          1141 ACGTTACCCA ACTTAAATCG CCCTTGCCAG GCAC
          //
          Primer: SV40pro_R
          Sequence file: RDB13379_A5E8g.seq check
          >13379_13379_A9D8F_3_SV40pro_R_B08_05_ABI24.ab1
              1 GGGGGGGCCC TTGACATAAT AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG
             61 GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA TAGCTAGAGC CAGACATGAT
            121 AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT
            181 TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT
            241 TCCTCTCACT CTCTGATATT CATTTCTTTG CAAGTTATAA ATACTGAATA ATAAGATGAC
            301 ATGAACTACT ACTGCTAGAG ATTTTCCACA CTGACTAAAA GGGTCTGAGG GATCTCTAGT
            361 TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG CACTCAAGGC AAGCTTTATT
            421 GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC CCAGGCTCAG ATCTGGTCTA
            481 ACCAGAGAGA CCCAGTACAA GCAAAAAGCA GATCTTATCT TCGTTGGGAG TGAATTAGCC
            541 CTTCCAGTCC CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA
            601 TTGGTCTTAA AGTCGAGTTA CTGCTCGTTC TTCAGCACGC GCTCCACGAA GCTCTTGATG
            661 TACTTACCCA TTTCATCTGG AGCGTCCTCC TGGAGGAAGT GGAGGCCCTT CACCTTCACG
            721 AACTCGGTGT TAGGGAACTT CTTAGCTCCC TCGACAATAG CGTTGGAAAA GAACCCAGGG
            781 TCGGACTCGA TGAACAGCTT AGGCAGATCG TCGCTGGCCC GAAGGTAGGC GTTGTAGTTG
            841 CGGACAATCT GGGACGACGT CGGGCTTGCC TCCCTTAACG AAGAGGGGAT CTCGCGAGGC
            901 CAGGAAGAGG GGTAGGCCGT CTAACCTCGC CCCTTCTCCT TGAAATGGGC TCCAGTAGGC
            961 AGCGAAACTT CCTCAGGCTC CAGTTTCCCG CATGAATCCT TGGCTTGGGC AACAGGGTCT
           1021 CGACAAAGAA GATTATTTCT TCAAGCACCA TTTTCTCGGC CCTTCTCGCT CCTTGGATTC
           1081 AAGGGCCCAG CTTCCCTTCC TTCGAATGTC CAGGCCAACT CCCAATTCCA GGACTTCGAT
           1141 CACGATATCC CACGCAACAC ATCTTCCCAT TAGTTGCACG CGAATATGAG CCCCCTATTG
          1201 GTA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Takai, A., Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging. Proc. Natl. Acad. Sci. U.S.A. 112 (14): 4352-4356 (2015). PMID 25831507.
          reference Saito, K., Recent progress in luminescent proteins development. Curr. Opin. Chem. Biol. 27: 46-51 (2015). PMID 26094043.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl