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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEMHE-mEGFP-INCENP (#RDB13519)

Plasmid for in vitro transcription of human INCENP in mouse oocyte.

Clone info. Plasmid for in vitro transcription of human INCENP in mouse oocyte. Fused with mEGFP.
Comment pGEMHE vector contains Xenopus laevis beta-globin 5' and 3' UTR.
Vector backbone pGEMHE (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name human INCENP cDNA Aequorea victoria GFP cDNA Xenopus laevis beta-globin cDNA
Depositor|Developer Kitajima, Tomoya |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, S., Dev. Cell 33 (5): 589-602 (2015))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13519 pGEMHE-mEGFP-INCENP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEMHE-mEGFP-INCENP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13519).

Reference section:

Yoshida, S., Kaido, M., Kitajima, T.S., Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes. Dev. Cell 33 (5): 589-602 (2015). PMID 26028219. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13519_B0Jup1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mVenus-M_F (Pr0574)
Region: insert mid (mEGFP 3', INCENP 5')
Sequence file: RDB13519_B0Jua.seq check
>13519_13519_B0Ju_1_mVenus-M_F_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNANNNGGN NTCNNGGTGN NCTTCNNGAN CCGCCNCNAC ATCGAGGACG
   61 GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC
  121 TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA
  181 AGCGCGATCA CATGGTCCTT AAGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG
  241 ACGAGCTGTA CAAGTACTCA GATCTCGAGC TCAAGCTTCG AATTCTGCAG TCGACGGCCA
  301 CCATGGGGAC GACGGCCCCA GGGCCCATTC ACCTGCTGGA GCTATGTGAC CAGAAGCTCA
  361 TGGAGTTTCT CTGCAACATG GATAATAAGG ACTTGGTGTG GCTTGAGGAG ATCCAAGAGG
  421 AGGCCGAGCG CATGTTCACC AGAGAATTCA GCAAAGAGCC AGAGCTGATG CCCAAAACAC
  481 CTTCTCAGAA GAACCGACGG AAGAAGAGAC GGATTTCTTA TGTTCAGGAT GAAAACAGAG
  541 ATCCCATCAG GAGAAGGTTA TCCCGCAGAA AGTCTCGGAG CAGCCAGCTG AGCTCCCGAC
  601 GCCTCCGCAG CAAGGACAGT GTAGAGAAGC TGGCTACAGT GGTCGGGGAG AACGGCTCCG
  661 TTCTGCGGCG TGTGACCCGT GCTGCGGCTG CAGCTGCCGC GGCTACCATG GCATTGGCTG
  721 CACCTTCTTC ACCCACCCCT GANTCTCCCA CGATGCTGAC TAANAAGCCC GANGATAACC
  781 ACACCCAGNG CCAGCTGGTG CCTGTGGNGG AGATCGGNAT CAGTGAGCGN CNGAATGCTG
  841 ANCANCATGT CACCCAGCTC ATGTCCNCCG ANCCTCNGCC CCGCANNCTG NCCCCGANTC
  901 CNGCTTCNNN NNNNGCTNCN ANCNTCCCAG GGNATCCCGA NNTCNNANGA NGNNNCNNNN
  961 NCNTANGNNG NNNAANGNCN NNNANACTNN NNNCCANNNN NNNGANNNNC NNNNNNNNNN
//
Primer: RDB13509#1 (Pr0307)
Region: insert mid (Xenopus globin 3'-UTR, INCENP 3')
Sequence file: RDB13519_B0Jub.seq check
>13519_13519_B0Ju_1_RDB13509#1_G03_21_ABI24.ab1
    1 NNNNNNNNNN NNNNANNNGT NNNNNGGTAT TATGTAGCTT AGAGACTCCA TTCGGGTGTT
   61 CTTGAGGCTG GTTTAGTGGT AACCAGATCA AGCTATCATT TATCTAGAGC GGCCGCTTTA
  121 CTTGTACAGG ATCCGCCTAG TGCTTCTTCA GGCTGTAGGC CAGGCTGCTG GGGACCCTGG
  181 CGCCCTGCAG GGGCGGTGAG TTCCAGACAG CAGAGCTGGT GCGCTTGTGA TAGCGGGGCT
  241 TGCTCTTCTT GAAGATATCC TCCAAGTCCA GTGGGAGAAT GGTTCCAAAG AGCTCCAGAA
  301 GGTTCGGTGG GTGGTAGTAC TGGTGAATGA TAGCCTGGCT GAGCGGGGTG CCTCGGGCCC
  361 AGGTGGGGAT GGGCTTCCGG GGATGGGCCT CATCATCGGT GGAGTCGTCG CTATTCAGAT
  421 CCATCCCGTA GTTATCTGGG TTGATCTTGG GAGGGGCCCT GTGCCCTTGC GGAGTCATCT
  481 GATATGAGGT ACAAGCTGGA GACTGCACGT CCACAGTCAC ATTCAGGGCC TTGCTGGCCC
  541 CTGCTGCCTC CTTGGCTTTC TTCTCTTGCT CCTCCTGCAG CTGCCGCTCA GCCAGACGCT
  601 GCTGCTCTTC CTTCTTCTTC TTCTCCTCCA GTTCCCTCTG CAGCTGCTCC TTCTGCAGCC
  661 GCANGGCCTT CTCCCGCTCC TGCAGCTNCC TCTCNGNCCT NNNCCGCTCC NGNTNCCGNN
721 NANNCNNNNN TCNNNNNNNN NN
//
Primer: HB-NOS_R (Pr0800)
Region: T7 pro, insert 5' (Xenopus globin 5'-UTR, mEGFP 5')
Sequence file: RDB13519_B0Juc.seq check
>13519_13519_B0Ju_1_HB-NOS_R_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNGNGNNN NNTTGGNNNN NCCNGGGTTT TCCCAGTCNC GACGTTGTAA
   61 AACGACGGCC AGTGAATTGT AATACGACTC ACTATAGGGC GAATTAATTC GAGCTCGGTA
  121 CCCAGCTTGC TTGTTCTTTT TGCAGAAGCT CAGAATAAAC GCTCAACTTT GGCAGATCAA
  181 TTCCCCGGTG CTAGCGCTAC CGGTCGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC
  241 CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT
  301 GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC
  361 CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA
  421 GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC
  481 CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG
  541 CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA
  601 CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA
  661 CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA
  721 CAACATCGAG GANGGCAGCG TGCAGCTCGC CGACCACTAC NAGCAGANCA CCCCCATCGG
  781 CGACNGCCCC GTGCTGCTGC CCGACNACCA CTACCTGANC ACCCAGTCCG CCCTGANCAA
  841 AGACCCCAAC GAGAANCGCG ATNACATGGT NCTTAAGGAG TTCGTGANNG CCGCCGGGNT
  901 CACTCTCGGC ANGGNNGANC NGTACNAGTA CTCANATCTC GAGCTNNNGC TTCNNNNTCN
  961 GCANNCNANN NNCNNCNNNG NNNNNGANNG NNNNAGGGNC CATNNNNCNN GCNNNNNNNN
1021 NN
//
Primer: SV40pA3'element Reverse (Pr0108)
Region: SP6 pro
Sequence file: RDB13519_B0Jud.seq check
>13519_13519_B0Ju_1_SV40pA3'elementReverse_H03_24_ABI24.ab1
    1 NNNNNNNGNN NNNNNNNNGN NNNNGCCNGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT
   61 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT
  121 ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG
  181 TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG
  241 CCGATTCATT AATGCAGCTG GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC
  301 AACGCAATTA ATGTGAGTTA GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT
  361 CCGGCTCGTA TGTTGTGTGG AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT
  421 GACCATGATT ACGCCAAGCT ATTTAGGTGA CACTATAGAA TACTCAAGCT AGATTAATTA
481 ATTTAAATCG CCGGCGGCGC GCCTGCAGGG GGGGGGGGGG NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yoshida, S., Inherent Instability of Correct Kinetochore-Microtubule Attachments during Meiosis I in Oocytes. Dev. Cell 33 (5): 589-602 (2015). PMID 26028219. [link to RRC of NBRP]