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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMAL/MBP-lysenin (#RDB13958)

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of MBP-tagged lysenin.

Clone info. Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of MBP-tagged lysenin.
Comment Expression was confirmed by the depositor.
Vector backbone pMAL-c2X (Plasmid)
Size of vector backbone 6.6 kb
Selectable markers Amp^r
Gene/insert name Eisenia foetida lysenin cDNA
Depositor|Developer Kobayashi, Toshihide | Abe, Mitsuhiro |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kiyokawa, E., Biochemistry 43 (30): 9766-9773, 2004.)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kiyokawa, E., Biochemistry 43 (30): 9766-9773, 2004.)。
備考 タンパク質毒素遺伝子です (シマミミズ lysenin)。EK1 (組換え大腸菌) 以外の使用は、「二種省令別表第一第一号ト」とポジションペーパーを参照してください。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB13958 pMAL/MBP-lysenin DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMAL/MBP-lysenin was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13958).

Reference section:

Kiyokawa, E., Makino, A., Ishii, K., Otsuka, N., Yamaji-Hasegawa, A., Kobayashi, T., Recognition of sphingomyelin by lysenin and lysenin-related proteins. Biochemistry 43 (30): 9766-9773 (2004). PMID 15274631. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13958_A6Btp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MBP_937-993 (Pr0340)
Region: MBP 3',Factor Xa site,insert 5'
Sequence file: RDB13958_A6Bta.seq check
>D03144A1_A6Bt_1_MBP_937_993_A04_01.ab1
    1 CCCAATTCGC TTTCTGGTAT GCCGTGCGTA CTGCGGTGAT CAACGCCGCC AGCGGTCGTC
   61 AGACTGTCGA TGAAGCCCTG AAAGACGCGC AGACTAATTC GAGCTCGAAC AACAACAACA
  121 ATAACAATAA CAACAACCTC GGGATCGAGG GAAGGATTTC AGAATTCGGA TCCATGTCGG
  181 CTAAAGCAGC AGAGGGATAT GAACAGATAG AAGTAGATGT GGTGGCAGTA TGGAAAGAAG
  241 GCTATGTATA CGAAAATCGC GGAAGCACCA GTGTGGATCA GAAGATCACA ATAACAAAAG
  301 GCATGAAAAA TGTGAATTCA GAAACAAGGA CAGTGACTGC TACGCATAGT ATTGGTTCTA
  361 CTATTAGCAC TGGAGATGCA TTCGAAATTG GAAGCGTGGA GGTTAGCTAC AGCCATTCAC
  421 ATGAAGAATC CCAAGTTAGC ATGACGGAAA CTGAAGTTTA TGAATCAAAG GTGATCGAAC
  481 ACACTATAAC GATTCCACCT ACTTCAAAAT TCACAAGATG GCAACTGAAT GCTGACGTTG
  541 GTGGAGCGGA TATTGAATAC ATGTATTTGA TTGATGAAGT CACACCCATA GGAGGGACTC
  601 AGAGTATTCC ACAGGTCATC ACAAGTCGGG CTAAAATTAT AGTTGGCCGA CAGATAATCC
  661 TTGGAAAAAC AGAAATTCGA ATTAAGCATG CAGAAAGGAA GGAGTACATG ACAGTCGTTT
  721 CAAGAAAAAG TTGGCCAGCT GCAACTCTTG GACATAGCAA ACTTTTCAAG TTTGTGCTCT
  781 ATGAAGATTG GGGGGGATTT CGAATTAAAA CGCTGAACAC CATGTATTCG GGCTATGAGT
  841 ATGCCTATTC CTCTGATCAA GGAGGAATCT ACTTTGATCA GGGTACTGAT AATCCGAAAC
  901 AGCGCTGGGC AATCAATAAG TCATTGCCTC TTCGTCATGG TGACGTAGTC ACCTTCATGA
  961 ATAAGTACTT CACTCGCAGT GGGCTGTGCT ACGATGATGG ACCGGCAACA AACGTGTACT
 1021 GTCTGGGACA ACGTGAAGAC AGTGGATTTG GAAGTGGTTG GTACCCCTGA ATGGGTGTCA
 1081 TGATGGTCAG CAGCATCACA TTCGCAATTG GCCTTCTGGT CATAATATTT CCACCTTTAA
1141 GCCACATGGA ATTGAG
//
Primer: pTrcHis-R (Pr0095)
Region: insert 3'
Sequence file: RDB13958_A6Btb.seq check
>D03144A1_A6Bt_1_pTrcHis_R_B12_06.ab1
    1 CGGGGGGGGC GGGCTGATCT TATCTCATCC GCCAAAACAG CCAAGCTGCC ATTCGCCATT
   61 CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT
  121 GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC
  181 ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTTCCGCTT TAGTTGCACC TCATCATTGT
  241 GCTAAAGGTG AAATATATGA CAGAAGCAAA TGCGAAATGG TGATGCTTGC TGACCAATCA
  301 TGACACCCCA TTTCAGGGGT TAACCAACCA CTTCCAAAAT CCACTTGTCT TCACGTTTGT
  361 CCAGACAGTA CACGTTTGTT GCCGGTCCAT CATCGTAGCA CAGCCCACTG CGAGTGAAGT
  421 ACTTATTCAT GAAGGTGACT ACGTCACCAT GACGAAGAGG CAATGACTTA TTGATTGCCC
  481 AGCGCTGTTT CGGATTATCA GTACCCTGAT CAAAGTAGAT TCCTCCTTGA TCAGAGGAAT
  541 AGGCATACTC ATAGCCCGAA TACATGGTGT TCAGCGTTTT AATTTCGAAA TCCCCCCCAA
  601 TCTTCATAGA GCACAAACTT GAAAAGTTTG CTATGTCCAA GAGTTGCACC TGGGCACTTT
  661 TTCTTGAAAC GACTGTCATG TACTCCTTCC TTTCTGCTGC TTTAATCCGA ATTTCCGTTT
  721 TTCCAAGGAT TATCTGTCGG CAATTATAAT TTTAGCCGAA CTGGGGATGA CCTGTGGAAT
781 AGTCTAAGTT CCTC
//
Primer: lacI_C_F (Pr0272)
Region: lacI,lac pro,lac O,MBP 5'
Sequence file: RDB13958_A6Btc.seq check
>D03144A1_A6Bt_1_lacI_C_F_A12_03.ab1
    1 AACAATAGCA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC TGGCACGACA
   61 GGTTTCCCGA CTGGAAAGCG GGCAGTGAGC GCAACGCAAT TAATGTAAGT TAGCTCACTC
  121 ATTAGGCACA ATTCTCATGT TTGACAGCTT ATCATCGACT GCACGGTGCA CCAATGCTTC
  181 TGGCGTCAGG CAGCCATCGG AAGCTGTGGT ATGGCTGTGC AGGTCGTAAA TCACTGCATA
  241 ATTCGTGTCG CTCAAGGCGC ACTCCCGTTC TGGATAATGT TTTTTGCGCC GACATCATAA
  301 CGGTTCTGGC AAATATTCTG AAATGAGCTG TTGACAATTA ATCATCGGCT CGTATAATGT
  361 GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CCAGTCCGTT TAGGTGTTTT
  421 CACGAGCACT TCACCAACAA GGACCATAGC ATATGAAAAT CGAAGAAGGT AAACTGGTAA
  481 TCTGGATTAA CGGCGATAAA GGCTATAACG GTCTCGCTGA AGTCGGTAAG AAATTCGAGA
  541 AAGATACCGG AATTAAAGTC ACCGTTGAGC ATCCGGATAA ACTGGAAGAG AAATTCCCAC
  601 AGGTTGCGGC AACTGGCGAT GGCCCTGACA TTATCTTCTG GGCACACGAC CGCTTTGGTG
  661 GCTACGCTCA ATCTGGCCTG TTGGCTGAAA TCACCCCGGA CAAAGCGTTC CAGGACAAGC
  721 TGTATCCGTT TACCTGGGAT GCCGTACGTT ACAACGGCAA GCTGATTGCT TACCCGATCG
  781 CTGTTGAAGC GTTATCGCTG ATTTATAACA AAGATCTGCT GCCGAACCCG CCAAAAACCT
  841 GGGAAGAGAT CCCGGCGCTG GATAAAGAAC TGAAAGCGAA AGGTAAGAGC GCGCTGATGT
  901 TCAACCTGCA AGAACCGTAC TTCACCTGGC CGCTGATTGC TGCTGACGGG GGTTATGCGT
  961 TCAAGTATGA AAACGGCAAG TACGGACATT AAGGACGTGG GCGTGGGATA ACGCTGGCGC
 1021 GAAAGGCGGG TCTGACCTTC CTGGTGACCT GAATTAAAAA CAACCACATT GATGCAGACA
 1081 CCGATAACTC CATTCGGCAG AAGCTTGCCA TTTATAAAGG CGAACCAGAC CGGAATGTGG
1141 AC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kiyokawa, E., Recognition of sphingomyelin by lysenin and lysenin-related proteins. Biochemistry 43 (30): 9766-9773 (2004). PMID 15274631. [link to RRC of NBRP]