Please review the QC test results indicated by icon below as well as clone information before placing your order.

pET28/PlyA2-EGFP-His6

Marker of sphingomyelin and cholesterol lipid domains. Bacterial expression plasmid of EGFP-His6-tagged PlyA2.

Sequence (full)	RDB13962zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Bhat, HB., J Lipid Res. 54 (10): 2933-2943, 2013.)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Bhat, HB., J Lipid Res. 54 (10): 2933-2943, 2013.)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

References and tips

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13962_A6Btp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBR322_BamHI_R (Pr0065) Region: RBS, insert 5' (PlyA2, EGFP 5') Sequence file: RDB13962_A6Btd.seq
>D03144A5_A6Bt_1_pBR 322 BamHI R_D04_10.ab1     1 NNNNNNNNNN NNNGNNTNNN NNTANGGNNN NTGTGAGCGG NNAACAATTC CCCTCTAGAA    61 ATAATTTTGT TNAACTTTAA GAAGGAGATA TACCGGTACC ATGGCATACG CACAATGGGT   121 CATCATCATC ATCCACAACG TCGGGTCCAA AGACGTGAAG ATCGTAAACC TCAAGCCTTC   181 TTGGGGGAAG CTTCATGCCG ACGGCGACAA AGACACAGAA GTCTCAGCAA GCAAGTACGA   241 AGGAACAGTC ATCAAGCCTG ACGAGAAGCT CCAGATCAAT GCCTGCGGCA GATCAGACGC   301 CGCCGAAGGC ACGACAGGAA CCTTCGACCT TGTTGACCCC GCCGATGGCG ACAAGCAGGT   361 CCGCCACTTC TACTGGGACT GCCCATGGGG AAGCAAGGCC AACACTTGGA CCGTTAGCGG   421 TTCCAACACC AAGTGGATGA TCGAGTACTC TGGCCAGAAC TTGGACAGCG GTGCCCTCGG   481 CACGATCACC GTCGACACCT TGAAGAAGGG GAACGAATTC GGAGGAGGAG GAGGAGGATC   541 CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA   601 CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA   661 TGGCAAGCTG ANCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC   721 CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA   781 GCAGCACGAC TTCTTCNAGT CCGCCATGCC CGAANGCTAC GTCCAGGANC GCANCATCTT   841 CTTCNNNGAC GANNGNNACT ACAAGANCCG CGCCNANGTG NANTTCGNNG GCGANNCCCN   901 GNNNNNNCNC ATCNAGCTGN ANNGNNNTCN NNTNNNNNNN NNANNNNNNC ATNCNGGGGN 961 ANNNGCTGGN NTNNNNCTNN NNNNNNNN //
Primer: EGFP-N_F (Pr0649) Region: insert 3' (EGFP 3'), 6xHis Sequence file: RDB13962_A6Bte.seq
>13962_13962_B2D1F_1_EGFP-N_F_A01_01_ABI08.ab1     1 NNNNNNNNNN NGNNNNNNNG NNGGCGNNGC NNCCTATGGC AAGCTGNNCC TGAAGTTCAT    61 CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG   121 CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC   181 CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA   241 GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG   301 CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG   361 CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT   421 CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC   481 CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT   541 GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC   601 CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGAAGCTT CACCACCACC ACCACCACTG   661 AGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGANTTG GCTGCTGCCA CCGCTGAGCA   721 ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGANAGG   781 AGGAACTATA TCCGGANTGN CGAATGGGAC GCGCCCNGTA GCGGCGCATT AAGCGCGGCG   841 GGTGTGGTGG TTACGCGCAG CGNGACCGCT ACACTTGCCA GCGCCCTANC GCCCGCTNCT   901 TTCGCTTTCN NCCCTTNCTT NCNCGCNNCN TNCGCCNGCT TNCCCCGNNN NGCTCNAANN 961 CNGGGGNNNN CNNTNNNGNN NNNNNNTNNN NNNNTNNNNN NNNNN //
Primer: EGFP-M_R (Pr0743) Region: insert mid (EGFP 5', PlyA2 3') Sequence file: RDB13962_A6Btf.seq
>13962_13962_B2D1F_1_EGFP-M_R_A12_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGGCGGNNC TNNNNNNNNC CTTGATGCCG TTCTTCTGCT    61 TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA   121 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC   181 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC   241 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT   301 CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG GTGGGCCAGG   361 GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCA TAGGTGGCAT   421 CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA   481 CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG GATCCTCCTC   541 CTCCTCCTCC GAATTCGTTC CCCTTCTTCA AGGTGTCGAC GGTGATCGTG CCGAGGGCAC   601 CGCTGTCCAA GTTCTGGCCA GANTACTCGA TCATCNACTT GGTGTTGGAA CCGCTNACGG   661 TCCAAGTGNT GGNCTTGCTN CCCCNTGGGN AGTCCNNGTA NANNNGGNNN ANNNGCTNNN 721 CGNNNTCGGC GGGNNNNNNN NNN //
Primer: pGL3_RV4 (Pr0335) Region: lacI 3' Sequence file: RDB13962_A6Btg.seq
>D03144A5_A6Bt_1_pGL3_RV4_G12_21.ab1     1 NNNNNNNNNN NNNNNNGNTC TCAGGGCNTC GGTCGANNNC CCGGTGCCTA ATGAGTGAGC    61 TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC   121 CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG   181 GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC   241 CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT   301 GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC   361 CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC   421 CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT   481 GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT   541 TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT   601 TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGANCCAAT GCGACCAGAT GCTCCACGCC   661 CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGANAC   721 ATCAAGAAAT AACGCCNGAA CATTAGTGCA NGCAGCTTCC ACAGCAATGG CATCCTGGTC   781 ATCCAGCGGA TAGTTAATGA TCAGCCCANN GANGCGTTGC GCGAGAAGNN TGTGCANCGC   841 CGCTTTNCAG GCNTCNACGC CGCTTCNTTC TANCNTCNAC NCCACCACNC NNNNACCCAG 901 TTGNNNNNNG NNANATTTNA TCGNNNNNAN NNNTTGCNAN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.12

GNP_filter3_RDBDEP_html_230512.pl