Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pClneoLuc-PP1A

Expression vector of luciferase-fused PP1A.

Catalog number RDB13971
Resource name pClneoLuc-PP1A
Clone info. Expression vector of luciferase-fused PP1A.
Comment Expression was confirmed by the depositor with luciferase activity. Known difference to PP1A (NM_002708.3): A to G at 799nt (Thr 226 Ala).
Vector backbone pCIneo (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human PP1A cDNA firefly luciferase
Depositor|Developer Hata, Yutaka |
Other clones in our bank

External Database
human PP1A firefly luciferase

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kawano, S., J. Biochem. 158 (5): 413-423, 2015)
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kawano, S., J. Biochem. 158 (5): 413-423, 2015)。
          付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB13971 pClneoLuc-PP1A DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kawano, S., A cell-based screening for TAZ activators identifies ethacridine, a widely used antiseptic and abortifacient, as a compound that promotes dephosphorylation of TAZ and inhibits adipogenesis in C3H10T1/2 cells. J. Biochem. 158 (5): 413-423 (2015). PMID 25979969.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13971_A6Btp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB13971_A6Bta.seq check
          >D03116A5_A6Bt_2_CMV_Forward_E07_13.ab1
              1 GATCGCTGCG ATCGCGCGCC CCGTTGTACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG
             61 GTCTATATAA GCAGAGCTCG TTTAGTGAAC CGTCAGATCA CTAGAAGCTT TATTGCGGTA
            121 GTTTATCACA GTTAAATTGC TAACGCAGTC AGTGCTTCTG ACACAACAGT CTCGAACTTA
            181 AGCTGCAGTG ACTCTCTTAA GGTAGCCTTG CAGAAGTTGG TCGTGAGGCA CTGGGCAGGT
            241 AAGTATCAAG GTTACAAGAC AGGTTTAAGG AGACCAATAG AAACTGGGCT TGTCGAGACA
            301 GAGAAGACTC TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC ACTTTGCCTT
            361 TCTCTCCACA GGTGTCCACT CCCAGTTCAA TTACAGCTCT TAAGGCTAGA GTACTTAATA
            421 CGACTCACTA TAGGCTAGCC ACCATGGAAG ACGCCAAAAA CATAAAGAAA GGCCCGGCGC
            481 CATTCTATCC GCTGGAAGAT GGAACCGCTG GAGAGCAACT GCATAAGGCT ATGAAGAGAT
            541 ACGCCCTGGT TCCTGGAACA ATTGCTTTTA CAGATGCACA TATCGAGGTG GACATCACTT
            601 ACGCTGAGTA CTTCGAAATG TCCGTTCGGT TGGCAGAAGC TATGAAACGA TATGGGCTGA
            661 ATACAAATCA CAGAATCGTC GTATGCAGTG AAAACTCTCT TCAATTCTTT ATGCCGGTGT
            721 TGGGCGCGTT ATTTATCGGA GTTGCAGTTG CGCCCGCGAA CGACATTTAT AATGAACGTG
            781 AATTGCTCAA CAGTATGGGC ATTTCGCAGC CTACCGTGGT GTTCGTTTCC AAAAATGGGT
            841 TGCAAAAAAT TTTGAACGTG CAAAAAAAGC TCCCAATCAT CCAAAAAATT ATTATCATGG
            901 ATTCTAAAAC GGATTACCAG GGATTTCAGT CGATGTACAC GTTCGTCACA TCTCATCTAC
            961 TCCCGGTTTT ATGATACGAT TTTGTGCCAG AGTCCTTCGA TAGGGACAGA CATGCACTGA
           1021 TCATGACTTC TCTGAATCTA CTGATCTGCT TAATGTGTCG CTCTGGCCTC ATAGAACTGC
          1081 CTGCTGAATC CGCATGCCGA GATCTAACTA TTGGCAATCC A
          //
          Primer: Luc-C_F
          Sequence file: RDB13971_A6Btb.seq check
          >D03116A5_A6Bt_2_Luc-C_F_G12_21.ab1
              1 TAGGCATCGT CGCCAGTCAG TAACAACCGC GAAAAAGTTG CGCGGAGGAG TTGTGTTTGT
             61 GGACGAAGTA CCGAAAGGTC TTACCGGAAA ACTCGACGCA AGAAAAATCA GAGAGATCCT
            121 CATAAAGGCC AAGAAGGGCG GAAAGATCGC CGTGGAATTC ATGTCCGACA GCGAGAAGCT
            181 CAACCTGGAC TCGATCATCG GGCGCCTGCT GGAAGTGCAG GGCTCGCGGC CTGGCAAGAA
            241 TGTACAGCTG ACAGAGAACG AGATCCGCGG TCTGTGCCTG AAATCTCGGG AGATTTTTCT
            301 GAGCCAGCCC ATTCTTCTGG AGCTGGAGGC ACCCCTCAAG ATCTGCGGTG ACATACACGG
            361 CCAGTACTAC GACCTTCTGC GACTATTTGA GTATGGCGGT TTCCCTCCCG AGAGCAACTA
            421 CCTCTTTCTG GGGGACTATG TGGACAGGGG CAAGCAGTCC TTGGAGACCA TCTGCCTGCT
            481 GCTGGCCTAT AAGATCAAGT ACCCCGAGAA CTTCTTCCTG CTCCGTGGGA ACCACGAGTG
            541 TGCCAGCATC AACCGCATCT ATGGTTTCTA CGATGAGTGC AAGAGACGCT ACAACATCAA
            601 ACTGTGGAAA ACCTTCACTG ACTGCTTCAA CTGCCTGCCC ATCGCGGCCA TAGTGGACGA
            661 AAAGATCTTC TGCTGCCACG GAGGCCTGTC CCCGGACCTG CAGTCTATGG AGCAGATTCG
            721 GCGGATCATG CGGCCCACAG ATGTGCCTGA CCAGGGCCTG CTGTGTGACC TGCTGTGGTC
            781 TGACCCTGAC AAGGACGTGC AGGGCTGGGG CGAGAACGAC CGTGGCGTCT CTTTTGCCTT
            841 TGGAGCCGAG GTGGTGGCCA AGTTCCTCCA CAAGCACGAC TTGGACCTCA TCTGCCGAGC
            901 ACACCAGGTG GTAGAAGACG GCTACGAGTT CTTTGCCAAG CGGCAGCTGG TGACACTTTT
            961 CTCAGCTCCC AACTACTGTG GCGAGTTTGA CATGCTGGGC GCCCATGATG AGTGTGGACG
           1021 AGACCCTCAT GTGCTCTTTC CAGATCTCAG CCCCGCCGAC AAGACCAGGG AGGTACCGGC
          1081 AGTTCAGGTG GCCCTGGAAG CAG
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB13971_A6Btc.seq check
          >D03116A5_A6Bt_2_pAxCALNL_F1_F07_16.ab1
              1 AACTTTCATG TTATCTTATC ATGTCTGCTC GAAGCATTAA CCCTCACTAA AGGGAAGCGG
             61 CCGCCCGGGT CGACTATTTC TTGGCTTTGG CGGAATTGCG GGGTGGGGTG ATGGGTCGGC
            121 CTCCAGGGTT CAGGCCACTG AACTGCCCGT ACTTCCCCTT GTTCTTGTCG GCGGGCTTGA
            181 GGATCTGGAA AGAGCACATG AGGGTCTCGT CCACACTCAT CATGGCGCCA GCATTGTCAA
            241 ACTCGCCACA GTAGTTGGGA GCTGAGAAAA GTGTCACCAG CTGCCGCTTG GCAAAGAACT
            301 CGTAGCCGTC TTCTACCACC TGGTGTGCTC GGCAGATGAG GTCCAAGTCG TGCTTGTGGA
            361 GGAACTTGGC CACCACCTCG GCTCCAAAGG CAAAAGAGAC GCCACGGTCG TTCTCGCCCC
            421 AGCCCTGCAC GTCCTTGTCA GGGTCAGACC ACAGCAGGTC ACACAGCAGG CCCTGGTCAG
            481 GCACATCTGT GGGCCGCATG ATCCGCCGAA TCTGCTCCAT AGACTGCAGG TCCGGGGACA
            541 GGCCTCCGTG GCAGCAGAAG ATCTTTTCGT CCACTATGGC CGCGATGGGC AGGCAGTTGA
            601 AGCAGTCAGT GAAGGTTTTC CACAGTTTGA TGTTGTAGCG TCTCTTGCAC TCATCGTAGA
            661 AACCATAGAT GCGGTTGATG CTGGCACACT CGTGGTTCCC ACGGAGCAGG AAGAAGTTCT
            721 CGGGGTACTT GATCTTATAG GCCAGCAGCA GGCAGATGGT CTCCAAGGAC TGCTTGCCCC
            781 TGTCCACATA GTCCCCCAGA AAGAGGTAGT TGCTCTCGGG AGGGAAACCG CCATACTCAA
            841 ATAGTCGCAG AAGGTCGTAG TACTGGCCGT GTATGTCACC GCAGATCTTG AGGGGTGCCT
            901 CCAGCTCCAG AAGAATGGGC TGGCTCAGAA AAATCTCCCG AGATTTCAGG CACAGACCGC
            961 GGATCTCGTT CTCTGTCAGC TGTACATTCT TGCCAGGCCG CGAGCCCTGC ACTTCCAGCA
           1021 GCGCCCGATG AATCGAGTCC AGGTTTGAGC TTTCTCGCTG TCGACATGAT TCACGGCGAT
          1081 CTTCCGCCAT CATGGCTTTT ATGAAGGATT CTCCTCTGAA TTTTTTTTTT T
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB13971_A6Btd.seq check
          >D03116A5_A6Bt_2_SV40pro_F_V2_G07_19.ab1
              1 GAACCCTTTT GGAGCTAGGC TTTTGCAAAA GCTTGATTCT TCTGACACAA CAGTCTCGAA
             61 CTTAAGGCTA GAGCCACCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT
            121 TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC
            181 GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC
            241 GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC
            301 GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG
            361 GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC
            421 ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC
            481 CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT
            541 CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC
            601 AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG
            661 AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG
            721 GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC
            781 GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC
            841 GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGGTT CGAAATGACC
            901 GACCAAGCGA CGCCCAACCT GCCATCACGA TGGCCGCAAT AAAATATCTT TATTTTCATT
            961 ACATCTGTGT GTTGGGTTTT TTTGTGTGAA TCGATAGCGA TACGATCCGC GTATGGTGCA
           1021 CTCTCAGTAC CATCTGCTTC TGATGCCGCA TAGTTAAAGC AAGCCCCGGA CACCGACAAC
          1081 ACCGCTGACG CGCCTTGACC GGCCTGATCC TGCTCCCGGG CCAATTCCGC GCTT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kawano, S., A cell-based screening for TAZ activators identifies ethacridine, a widely used antiseptic and abortifacient, as a compound that promotes dephosphorylation of TAZ and inhibits adipogenesis in C3H10T1/2 cells. J. Biochem. 158 (5): 413-423 (2015). PMID 25979969.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl