Please review the QC test results indicated by icon below as well as clone information before placing your order.

pIDT-SMART(C-TSC)

Expression vector with the improved high expression cassette C-TSC.

Sequence (full)	RDB14136zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Sakaguchi, M. et al., Mol. Biotechnol. 56(7):621-630, 2014)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sakaguchi, M. et al., Mol. Biotechnol. 56(7):621-630, 2014)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14136_A6Dmp1-4.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: BGH_rev2 Region: bGH pA Sequence file: RDB14136_A6Dmk.seq
>D03160A6_A6Dm_1_BGH_rev2_C02_08.ab1     1 NNNNNNNNNN NNNNNNGNNN GGNNGNNGGG NAACAACAGA TGGCTGGCAA CTAGAAGGCA    61 CAGTCGAGGC TGATCAGCGG GTTTAAACGT TAGTCATCTA GCTCTAGAGC AAAAGGCCTT   121 TTCCGCTCGA GCGGCGCGGG AGGCTGCTGG TTTTCCACTA CCCGAAAAAA ATCCAGCGTC   181 TAAGCAGCTG CAAGGAGAGC CTTTCAGAGA AGCGGGTCCT GGCAGCGGCG GGGAAGTGTC   241 CCCAAATGGG CAGAATAGCC TCCCCGCGTC GGGAGAGTCG CGTCCTTGCT CGGGTGTTGT   301 AAGTTCCAGT GCAAAGTGCC CGCCCGCTGC TATGGGCAAA GTTTCGTGGA TGCGGCAAGG   361 GTTGCGGACC GCTGGCTGGG GGATCAGCGG GAGGGCTGGG CCAGAGGCGA AGCCCCCTAT   421 TCGCTCCGGA TCTCCCTTCC CAGGACGCCC GCAGCGCAGC TCTGCTCGCC CGGCTCTTCC   481 ACCCTAGCCG GCCGCCCGCT CGCTCCCTCT GCCTCTCGCT GGAATTAGGG GCTAGCTAAC   541 CCAAGCTTGG GCGCGGATCC GCGAGGATCG CGGCCACCAG GGAGAGCTTG ATCTTGCCAG   601 CCAGTAGGGC AGCAGCAGGC AGTCCAGCCA CAGGCCGTAG CACAGGAGCA CAGCGCAATT   661 TCGACTTGCA GGCGGCAGGG GCCCGGGGTC ACAAGGCGCC ACGAACCAGG CGAANGGCAG   721 GTCTAGTAAT ACAGGCCGCG GCGCTTCCCT CTCACACTCG CGNAACACCC CAATAGGTCA   781 ATCTGTCTGT GCTGTCTTGN NCGGCGTCGA ACTAGTCCTC GGGGGTACCC CNGGAAGATC   841 TTCNNCGGAA TTCCGGGGTG NCTNGGATCT GTANCGGCGC AGANCAGAAA ANGAAACAAA   901 GACGTAGANT TGANCAAGCN NGNTCNNNNA AAGCGTGGAG ANCNNGCNNA NNNTNNNTAN 961 GNNCCNANNG NNNCNNCNNN NNNANNNNCN NNNNNNNNN //
Primer: M13fwd Region: CMV 1(CMV en,CMV pro) Sequence file: RDB14136_A6Dml.seq
>D03160A6_A6Dm_1_M13fwd_C11_08.ab1     1 NNNNNNNNNN NNNNNNGNNN NNNNGNGNNC CNGTGNGTTG NNTGCAGTCC AGTTACGCTG    61 GANTCTGAGG CTCGTCCTGA ATGATACGCG TCGGAGGGTT GCGTTTGAGA CGGGCGACAG   121 ATCGACACTG CTCGATCCGC TCGCACCACG CGTCGACGTC GGCCATAAAT TTTTTGCAAA   181 AGCCTTGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG   241 CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCTTG GGGCGGAGAA ACTATCGTTG   301 CTGACTAATT GAGATCGGAG TACTGTCCTC CGCGTTACAT AACTTACGGT AAATGGCCCG   361 CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA   421 GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC   481 CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC   541 GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG   601 CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC   661 AATGGGCGTG GATAGCGGTT TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC   721 AATGGGAGTT TGTTTTGGCA CCAAAATCAA CNGGACTTTC CAAAATGTCG TANCAACTCC   781 GCCCCATTGA CGCAAATGGG CNGTANGCGT GTACNGTGGG GANGTCTATA TAAGCAGANC   841 TCGNTTNNGT GAACCGTCAG NTCGCCNGGN ANNNGNNNTC CACNCNNNTT TGANNNNNNN 901 ANNNNANNNN NNNNNN //
Primer: RU5-R Region: RU5',CMV 1(CMV pro,CMV en) Sequence file: RDB14136_A6Dmm.seq
>D03160A6_A6Dm_1_RU5-R_D05_11.ab1     1 NNNNNNNNCA NCNNNNCNGG CNAGCGTGGN NAGCCGGCTG NNTCTAGGTA GGCTCCAAGG    61 GAGCGCCGGA CAAAGGCCCG GTCTCGACCT GAGCTTTAAA CTTACCTAGA CGGCGGACGC   121 AGTTCAGGAG GCACCACAGG CGGGNGGCGG CAGAACGCGA CTCAACCGGC GTGGATGGCG   181 GCCTCAGGTA GGGCGGCGGG CGCGTGAAGG AGAGATGCGA GCCCCTCGAA GCCAGAAAAG   241 ACCCAGGAAA GGAACAGTCT GTCTCCCTAT AGTGAGTCGT ATTAATTGAT GGATAAGAGC   301 CAAAGGGGTG TGCCTATAGA GTCTATAGGC GGTACTTACG TCACTCTTGG CACGGGGAAT   361 CCGCGTTCCA ATGCACCGTT CCCGGCCGCG GAGGCTGGAT CGGTCCCGGT GTCTTCTATG   421 GAGGTCAAAA CAGCGTGGAT GGCGTCTCCA GGCGATCTGA CGGTTCACTA AACGAGCTCT   481 GCTTATATAG ACCTCCCACC GTACACGCCT ACCGCCCATT TGCGTCAATG GGGCGGAGTT   541 GTTACGACAT TTTGGAAAGT CCCGTTGATT TTGGTGCCAA AACAAACTCC CATTGACGTC   601 AATGGGGTGG AGACTTGGAA ATCCCCGTGA GTCAAACCGC TATCCACGCC CATTGATGTA   661 CTGCCAAAAC CGCATCACCA TGGTAATAGC GATGACTAAT ACGTAGATGT ACTGCCAAGT   721 ANGAAAGTCC CATAANGTCA TGTACTNNNC ATAATGCCAG GCGGGCCATT TACCGTCATT   781 GACGTCNATA NGGGGCGTAC TTGNNATATG ATACANTTGA TGNACTGCCA ANNNGGCAGT   841 TTANCGNAAA TACTCCANCN NNNNNCGNNN ANNGNAANNN CNNATNNNNG NNANNNNNGG 901 NANNNTACGN CATNNNNNNN NNN //
Primer: BGH_F2 Region: bGHp(A),hTERT,SV40 Sequence file: RDB14136_A6Dmn.seq
>D03160A6_A6Dm_1_BGH_F2_C05_08.ab1     1 NNNNNNNNNN NNNNNNNTTG NNCNTGGNNN GNGCCNCTCC CNCTGTCCTT TCCTAATAAA    61 ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG   121 GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG   181 GCTCTATGGC GGAGTACTGT CCTCCGCTTC CCACGTGGCG GAGGGACTGG GGACCCGGGC   241 ACCCGTCCTG CCCCTTCACC TTCCAGCTCC GCCTCCTCCG CGCGGACCCC GCCCCGTCCC   301 GACCCCTCCC GGGTCCCCGG CCCAGCCCCC TCCGGGCCCT CCCAGCCCCT CCCCTTCCTT   361 TCCGCGGCCC CGCCCTCTCC TCGCGGCGCG AGTTTTGGAA AGTCCCCAGG CTCCCCAGCA   421 GGCAGAAGTA TCCAAAGCAT CCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA   481 GGCTCCCCAG CAGGCAGAAG TATCCAAAGC ATCCATCTCA ATTAGTCAGC AACCATAGTC   541 CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC   601 CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGANG CCGCCTCTGC CTCTGAGCTA   661 TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCAAGGCT TTTGCAAAAA GCTCCGTTAC   721 ATAACTTACG GTAAATGGCC CGCCTGGCTG ANCGCCCAAC GACCCCCGCC CATTGACGTC   781 AATAATGACG TATGTTCCCA TANNAACGCC NATANGGNNT TTCCATTNAC GTCAATGNGT   841 GNANTANTTA CNGNAAANNG CCCANTNNGC ANNACATCNN NNGTATCATA NGCNAANNAC 901 NNCCCCCNAN NNNNNNNNN //
Primer: M13R Region: CMV 2(CMV en),SV40 Sequence file: RDB14136_A6Dmo.seq
>D03160A6_A6Dm_1_M13R_D11_11.ab1     1 NNNNNNNNNN NNNNNNNNGG NNNNNTCNTA CGTATAATGC CGTANGNNCG CGGCCGCCGG    61 NNGACAGCTC GCTCGTCCAG AACTGATCCA GTCGCGCTGC TCTCGTCGAT CCTTGGCGCG   121 CCAACACGCC TACCGCCCAT TTGCGTCAAT GGGGCGGAGT TGTTACGACA TTTTGGAAAG   181 TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT CAATGGGGTG GAGACTTGGA   241 AATCCCCGTG AGTCAAACCG CTATCCACGC CCATTGATGT ACTGCCAAAA CCGCATCACC   301 ATGGTAATAG CGATGACTAA TACGTAGATG TACTGCCAAG TAGGAAAGTC CCATAAGGTC   361 ATGTACTGGG CATAATGCCA GGCGGGCCAT TTACCGTCAT TGACGTCAAT AGGGGGCGTA   421 CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG TTTACCGTAA ATACTCCACC   481 CATTGACGTC AATGGAAAGT CCCTATTGGC GTTACTATGG GAACATACGT CATTATTGAC   541 GTCAATGGGC GGGGGTCGTT GGGCGGTCAG CCAGGCGGGC CATTTACCGT AAGTTATGTA   601 ACGGAGCTTT TTGCAAAAGC CTTGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG   661 CTCAGANGCA GANGCGGCCT CNGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG   721 CGGANAATGG GCGGAACTGG GCGGANTTAN GGGCGGGATG GGCGGANTTA GGGGCGGGAC   781 TATGGTTGCT GACTAATTGA GATGGATGCT TTGGATACTT CTGCCTGCTG GGGANCCNGG   841 GGACTTTCCA CACCTGGTTG CTGACNNANN TGAGATNGAT GCTTTNNATA CTTCNNNNNN   901 CNGGGGNNNC NTGGGGNNNT TTNNNNAAAC TCGCGCCNCN NNGNNNANGG NNGGGNNNNN 961 NNNNANNNNN NNNGNNNNGN NNNGNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.08.12

GNP_filter3_RDBDEP_html_220812.pl