Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGEX-GFP-ERK2-His

Bacterial expression vector of GFP–human ERK2.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Shindo, Y. et al., Nat. Commun. 7:10485, 2016)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Shindo, Y. et al., Nat. Commun. 7:10485, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14152_A6Ecp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-N Sequence file: RDB14152_A6Eca.seq
>D03441A1_A6Ec_2_EGFP_N_A04_01.ab1     1 GAACCGGGGG GGAGGCTCTC GCCTTGCTCA CCATCCCGGG ACCCTGGAAC AGAACTTCCA    61 GAGATCCACG CGGAACCAGA TCCGATTTTG GAGGATGGTC GCCACCACCA AACGTGGCTT   121 GCCAGCCCTG CAAAGGCCAT GCTATATACT TGCTGGATTT CAAGTACTTA TCAATTTGTG   181 GGATAGCTTC AATACGTTTT TTAAAACAAA CTAATTTTGG GAACGCATCC AGGCACATTG   241 GGTCCATGTA TAAAACAACA TCAAGAGCGT CATACAACAT GAAGTCAGGA TGGGTTACAT   301 GATCACCATT TAAATATGTT TTATGACATA AACGATCTTC GAACATTTTC AGCATTTCAG   361 GTAGCTTGCT AAGAAAATCA ACTTTGAGAG TTTCAAAGTC TTTACTATAT GCAATTCTCG   421 AAACACCGTA TCTAATATCC AAAACCGCTC CTTCAAGCAT TGAAATCTCT GCACGCTCTT   481 TTGGACAACC ACCCAACATG TTGTGCTTGT CAGCTATATA ACGTATGATG GCCATAGACT   541 GTGTTAATTT AACATCACCA TCAATATAAT AAGGAAGATT GGGAAACTCC AAACCCAATT   601 CAAACTTTTT GTTTCGCCAT TTATCACCTT CATCGCGCTC ATACAAATGC TCTTCATATT   661 TTTCTTCAAG ATATTCCAAA AGAAGTCGAG TGGGTTGCAC AAGGCCCTTA ATTTTCCAAT   721 AACCTAGTAT AGGGGACATG AATACTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT   781 CCACACATTA TACGAGCCGA TGATTAATTG TCAACAGCTC ATTTCAGAAT ATTTGCCAGA   841 ACCGTTATGA TGTCGGCGCA AAAAACATTA TCCAGAACGG GAGTGCGCCT TGAGCGACAC   901 GAATTATGCA GTGATTTACG ACCTGCACAG CCATACCACA GCTTCCGATG GGCTGCCTGA   961 CGCCAGAAGC ATTTGGTGCC ACCGTGCAGT CGATAAGCTT ATACGACGCA TCAAAATTAT 1021 CGCGTCTGGG CTTCCTTGTA GACAGCCTTC CATTCGACAT TAAAATGTGA AGCCGA //
Primer: EGFP-C Sequence file: RDB14152_A6Ecb.seq
>D03441A1_A6Ec_2_EGFP_C_B04_04.ab1     1 CAGGTATCTC GGGCATGGAC GAGCTGTACA AGTCCGGCCG GACTCAGATC CAAGCTTCGA    61 ATTCTGCAGT CGACGGTACC GCGGGCCCAT CAACAAGTTT GTACAAAAAA GCAGGCTTTA   121 TCCCGGCAGC CCATATGGCG GCGGCGGCGG CGGCGGGCGC GGGCCCGGAG ATGGTCCGCG   181 GGCAGGTGTT CGACGTGGGG CCGCGCTACA CCAACCTCTC GTACATCGGC GAGGGCGCCT   241 ACGGCATGGT GTGCTCTGCT TATGATAATG TCAACAAAGT TCGAGTAGCT ATCAAGAAAA   301 TCAGCCCCTT TGAGCACCAG ACCTACTGCC AGAGAACCCT GAGGGAGATA AAAATCTTAC   361 TGCGCTTCAG ACATGAGAAC ATCATTGGAA TCAATGACAT TATTCGAGCA CCAACCATCG   421 AGCAAATGAA AGATGTATAT ATAGTACAGG ACCTCATGGA AACAGATCTT TACAAGCTCT   481 TGAAGACACA ACACCTCAGC AATGACCATA TCTGCTATTT TCTCTACCAG ATCCTCAGAG   541 GGTTAAAATA TATCCATTCA GCTAACGTTC TGCACCGTGA CCTCAAGCCT TCCAACCTGC   601 TGCTCAACAC CACCTGTGAT CTCAAGATCT GTGACTTTGG CCTGGCCCGT GTTGCAGATC   661 CAGACCATGA TCACACAGGG TTCCTGACAG AATATGTGGC CACACGTTGG TACAGGGCTC   721 CAGAAATTAT GTTGAATTCC AAGGGCTACA CCAAGTCCAT TGATATTTGG TCTGTAGGCT   781 GCATTCTGGC AGAAATGCTT TCTAACAGGC CCATCTTTCC AGGGAAGCAT TATCTTGACC   841 AGCTGAACCA CATTTTTGGG TATTCTTGGA TCCCCCATCA CAAGAAAGAC CTGAAATTGT   901 ATAAATAAAA TTTTAAAAGC TAGGAAACTA TTTTGCTTTT CTCTTTCCCA CACAAAAAAT   961 AAAGGTGCCC ATGGAAACAG GCTGGTTTCC CAAATGCTGA CTCCCAAGCT CTGGACTTTA  1021 TTGGACAAAA TGGATGACCA TTCCAAACCC ACACAAGCAG ATCAGTTAGA CAGGCTCTGG 1081 GTCCGATCTC AA //
Primer: pGEX-R Sequence file: RDB14152_A6Ecc.seq
>D03441A1_A6Ec_2_pGEX_R_C04_07.ab1     1 TTTATTACGG TAACGCGCGA GGCAGATCGT CAGTCAGTCA CGATGAATTC AATGATGATG    61 ATGATGATGA TGATGACCGG TAGCACCAGA TCTGTATCCT GGCTGGAATC TAGCAGTCTC   121 TTCAAAAATT AGTTCTTTGA GCTTTTCCTT AGGCAAGTCA TCCAATTCCA TGTCGAACTT   181 GAATGGTGCT TCGGCGATGG GCTCGTCACT CGGGTCGTAA TACTGCTCCA GATATGGGTG   241 GGCCAGAGCC TGTTCTACTT CAATCCTCTT GTGTGGGTTG AATGTCAACA TTTTGTCCAA   301 TAAGTCCAGA GCTTTGGAGT CAGCATTTGG GAACAGCCTG TTCCATGGCA CCTTATTTTT   361 GTGTGGAAGA GAAAGCAAAT AGTTCCTAGC TTTTAAATTT ATTATACAAT TCAGGTCTTC   421 TTGTGATGGG GATCCAAGAA TACCCAAAAT GTGGTTCAGC TGGTCAAGAT AATGCTTCCC   481 TGGAAAGATG GGCCTGTTAG AAAGCATTTC TGCCAGAATG CAGCCTACAG ACCAAATATC   541 AATGGACTTG GTGTAGCCCT TGGAATTCAA CATAATTTCT GGAGCCCTGT ACCAACGTGT   601 GGCCACATAT TCTGTCAGGA ACCCTGTGTG ATCATGGTCT GGATCTGCAA CACGGGCCAG   661 GCCAAAGTCA CAGATCTTGA GATCACAGGT GGTGTTGAGC AGCAGGTTGG AAGGCTTGAG   721 GTCACGGTGC AGAACGTTAG CTGAATGGAT ATATTTTAAC CCTCTGAGGA TCTGGTAGAG   781 AAAATAGCAG ATATGGTCAT TGCTGAGGTG TTGTGTCTTC AAGAGCTTGT AAAGATCTGT   841 TTCCATGAGG TCCTGTACTA TATATACATC TTTTCATTTT GCTCGATGGT TGGTGCTCGA   901 ATAATGTCAT TGATTCCAAT GATGTTCTCA TGTCTGAAGC GCAGTAAGAT TTTTTATCTC   961 CCTCAGGGTT CTCTGGCAGT AGGTCTGGTG CTCAAAAGGG GCTGGATTTT TGTTGATAGC 1021 TACCTCGCAC CTTTGATTGG GACAGTATCC ATTAAGCAGA GCCACCACCG ATTGCCCGT //
Primer: SV40pA3'Element Reverse Sequence file: RDB14152_A6Ecd.seq
>D03441A1_A6Ec_2_SV40pA3'element_Reverse_D04_10.ab1     1 GAGGTTTGGG GGGGCGGGGC CTATGGAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT    61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA   121 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG   181 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA   241 TCTGTGCGGT ATTTCACACC GCATAAATTC CGACACCATC GAATGGCGCA AAACCTTTCG   301 CGGTATGGCA TGATAGCGCC CGGAAGAGAG TCAATTCAGG GTGGTGAATG TGAAACCAGT   361 AACGTTATAC GATGTCGCAG AGTATGCCGG TGTCTCTTAT CAGACCGTTT CCCGCGTGGT   421 GAACCAGGCC AGCCACGTTT CTGCGAAAAC GCGGGAAAAA GTGGAAGCGG CGATGGCGGA   481 GCTGAATTAC ATTCCCAACC GCGTGGCACA ACAACTGGCG GGCAAACAGT CGTTGCTGAT   541 TGGCGTTGCC ACCTCCAGTC TGGCCCTGCA CGCGCCGTCG CAAATTGTCG CGGCGATTAA   601 ATCTCGCGCC GATCAACTGG GTGCCAGCGT GGTGGTGTCG ATGGTAGAAC GAAGCGGCGT   661 CGAAGCCTGT AAAGCGGCGG TGCACAATCT TCTCGCGCAA CGCGTCAGTG GGCTGATCAT   721 TAACTATCCG CTGGATGACC AGGATGCCAT TGCTGTGGAA GCTGCCTGCA CTAATGTTCC   781 GGCGTTATTT CTTGATGTCT CTGACCAGAC ACCCATCAAC AGTATTATTT TTCTCCCATG   841 AAGACGGTAC GCGACTGGGC GTGGAGCATC TGGTCGCATT GGGTCACCAG CAAATCGCGC   901 TGTTAGCGGG CCCATTAAGT TCTGTCTCGG CGCGGTCTGC GTCTGGCTGG CTGGCATAAA   961 ATATCTCACT CGCAAATCAA ATTCAGCCGA TAGCGGAACG GAAGGCGACC TGGAAGTGCA 1021 TGTCCGGATT TTCAACAACC ATGCAAAATG CTTGAAAATG GAGGGCCCA //
Primer: M13R Sequence file: RDB14152_A6Ece.seq
>D03441A1_A6Ec_2_M13R_E04_13.ab1     1 GACGTGACGT CGTTTTTCAA CGTCGTGACT GGGAAACCCT GGCGTTACCC AACTTAATCG    61 CCTTGCAGCA CATCCCCCTT TCGCCAGCTG GCGTAATAGC GAAGAGGCCC GCACCGATCG   121 CCCTTCCCAA CAGTTGCGCA GCCTGAATGG CGAATGGCGC TTTGCCTGGT TTCCGGCACC   181 AGAAGCGGTG CCGGAAAGCT GGCTGGAGTG CGATCTTCCT GAGGCCGATA CTGTCGTCGT   241 CCCCTCAAAC TGGCAGATGC ACGGTTACGA TGCGCCCATC TACACCAACG TGACCTATCC   301 CATTACGGTC AATCCGCCGT TTGTTCCCAC GGAGAATCCG ACGGGTTGTT ACTCGCTCAC   361 ATTTAATGTT GATGAAAGCT GGCTACAGGA AGGCCAGACG CGAATTATTT TTGATGGCGT   421 TGGAATTAGC TTATCGACTG CACGGTGCAC CAATGCTTCT GGCGTCAGGC AGCCATCGGA   481 AGCTGTGGTA TGGCTGTGCA GGTCGTAAAT CACTGCATAA TTCGTGTCGC TCAAGGCGCA   541 CTCCCGTTCT GGATAATGTT TTTTGCGCCG ACATCATAAC GGTTCTGGCA AATATTCTGA   601 AATGAGCTGT TGACAATTAA TCATCGGCTC GTATAATGTG TGGAATTGTG AGCGGATAAC   661 AATTTCACAC AGGAAACAGT ATTCATGTCC CCTATACTAG GTTATTGGAA AATTAAGGGC   721 CTTGTGCAAC CCACTCGACT TCTTTTGGAA TATCTTGAAG AAAAATATGA AGAGCATTTG   781 TATGAGCGCG ATGAAGGTGA TAAATGGCGA AACAAAAAGT TTGAATTGGG TTTGGAGTTT   841 CCCAATCTTC CTTATTATAT TGATGGTGAT GTTAAATTAA CACAGTCTAT GGCCCATCAT   901 ACGTTATATA GCTGACAGCA CAACATGTTT GGGGTGGTTG TCCCAAAAGA GCGTGCAGAG   961 ATTTCAATGC TTTGAAGGAG CGGGTTTGGA TATTAGAATA CGTGTTTCGA GCACTGCCAT 1021 AATAGTAAGG ACGTGAACTC CTCGAAGATT GATTCTAAGC AAGCCTAACC GAT //
Primer: EGFP_N_rev Sequence file: RDB14152_A6Ecf.seq
>D03441A1_A6Ec_2_EGFP-N_rev_F04_16.ab1     1 ACTCAAGTTC AGCGTGTCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG    61 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG   121 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC   181 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC   241 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG   301 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC   361 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC   421 TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG   481 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG   541 TCCAAGCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG   601 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTCCGGCCG GACTCAGATC   661 CAAGCTTCGA ATTCTGCAGT CGACGGTACC GCGGGCCCAT CAACAAGTTT GTACAAAAAA   721 GCAGGCTTTA TCCCGGCAGC CCATATGGCG GCGGCGGCGG CGGCGGGCGC GGGCCCGGAG   781 ATGGTCCGCG GGCAGGTGTT CGACGTGGGG CCGCGCTACA CCAACCTCTC GTACATCGGC   841 GAGGGCGCCT ACGGCATGGT GTGCTCTGCT TATGATAATG TCAACAAAGT TCGAGTAGCT   901 ATCAAGAAAA TCAGCCCCTT TGAGCACCAG ACCCTACTGC CCAGAGAACC CTGAGGAGAT   961 AAAAATCTTA CCTGCCGCCT TCAGACATGA GACATCATGG TATCATGACA TATCGAGCAC 1021 AACATCGAGC AATGAAAGAT GTATATATAG TTACAGGATC GC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl