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Plasmid clone of constitutive active form of human MEK1.
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External Database human MEK1 Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Shindo, Y. et al., Nat. Commun. 7:10485, 2016) |
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| Ordering | Order form [Credit Card Payment ] [Bank Transfer Payment]MTA, for use for not-for-profit academic purpose [Word ]Please visit Information of Request for Distribution.[link] |
[open/close]| 提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Shindo, Y. et al., Nat. Commun. 7:10485, 2016)。 |
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| 提供依頼 | 提供依頼書 [Word ]提供同意書 (MTA, 非営利学術目的用)[Word ]手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB14154 | pACYC184-Venus-MEK1 (S218/222E, delta32-51) | DNA solution |
icon below as well as clone information before placing your order.Materials & Methods section:
| The pACYC184-Venus-MEK1 (S218/222E, delta32-51) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14154). |
Reference section:
| Shindo, Y., Iwamoto, K., Mouri, K., Hibino, K., Tomita, M., Kosako, H., Sako, Y., Takahashi, K., Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling. Nat. Commun. 7: 10485 (2016). PMID 26786866. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB14154_A6Ecp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: EGFP_N_rev Sequence file: RDB14154_A6Eca.seq
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>D03441A2_A6Ec_3_EGFP-N_rev_A11_01_ABI08.ab1 1 AGAATTAAAG GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA 61 CCCTGAAGCT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA 121 CCCTCGGCTA CGGCCTGCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG CAGCACGACT 181 TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG 241 ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA 301 TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT 361 ACAACTACAA CAGCCACAAC GTCTATATCA CAGCCGACAA GCAGAAGAAC GGCATCAAGG 421 CAAACTTCAA GATCCGCCAC AACATCGAGG ACGGCGGTGT GCAGCTCGCC GACCACTACC 481 AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCT 541 ACCAGTCCAA ACTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT 601 TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGGGT GGCGGTGGCG 661 GTGGCGGTGG CCTCGACCCC AAGAAGAAGC CGACGCCCAT CCAGCTGAAC CCGGCCCCCG 721 ACGGCTCTGC AGTTAACGGG ACCAGCTCTG CGGAGACCAA CTTGGAGGCC TTTCTTACCC 781 AGAAGCAGAA GTGGGAGAAC TGAAGATGAC GACTTTGAGA AGATCAGTGA GCTGGGGGGC 841 TGGCAATGGC CGGTGTGGTG TTCAAAGTCT CCCACAAGCC TCTGGGCCTG GTCATGGCCA 901 GAAAAGCTAA TTCATCTGGA AGATCAAACC CGGCAATCCG AACCAGATCA TAAGGAGGCT 961 GGCAAGTCTG CATGGAGTGC CACCTCTCCG TACATTCGTG GGCTTCTATG GGTGCGTTCT 1021 ACAGCGATGG CGGAGAATCA AGTATTCTTG GCATTGGGAG CCATTGATGA AGTCCTCTGG 1081 AAGTCAGTCC TGAGCAGCTG AAAT // |
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Primer: EGFP-C Sequence file: RDB14154_A6Ecb.seq
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>D03441A2_A6Ec_3_EGFP_C_B11_02_ABI08.ab1 1 CCGGGAATAT CTCGGGCATG GACGAGCTGT ACAAGGGTGG CGGTGGCGGT GGCGGTGGCC 61 TCGACCCCAA GAAGAAGCCG ACGCCCATCC AGCTGAACCC GGCCCCCGAC GGCTCTGCAG 121 TTAACGGGAC CAGCTCTGCG GAGACCAACT TGGAGGCCTT TCTTACCCAG AAGCAGAAGG 181 TGGGAGAACT GAAGGATGAC GACTTTGAGA AGATCAGTGA GCTGGGGGCT GGCAATGGCG 241 GTGTGGTGTT CAAGGTCTCC CACAAGCCTT CTGGCCTGGT CATGGCCAGA AAGCTAATTC 301 ATCTGGAGAT CAAACCCGCA ATCCGGAACC AGATCATAAG GGAGCTGCAG GTTCTGCATG 361 AGTGCAACTC TCCGTACATC GTGGGCTTCT ATGGTGCGTT CTACAGCGAT GGCGAGATCA 421 GTATCTGCAT GGAGCACATG GATGGAGGTT CTCTGGATCA AGTCCTGAAG AAAGCTGGAA 481 GAATTCCTGA ACAAATTTTA GGAAAAGTTA GCATTGCTGT AATAAAAGGC CTGACATATC 541 TGAGGGAGAA GCACAAGATC ATGCACAGAG ATGTCAAGCC CTCCAACATC CTAGTCAACT 601 CCCGTGGGGA GATCAAGCTC TGTGACTTTG GGGTCAGCGG GCAGCTCATC GACGAAATGG 661 CCAACGAATT CGTGGGCACA AGGTCCTACA TGTCGCCAGA AAGACTCCAG GGGACTCATT 721 ACTCTGTGCA GTCAGACATC TGGAGCATGG GACTGTCTCT GGTAGAGATG GCGGTTGGGA 781 GGTATCCCAT CCCTCCTCCA GATGCCAAGA GCTGGAGCTG ATGTTTGGGT GCCAGTGGAA 841 GGAGATGCGG CTGAGACCCC ACCAGGCCAA GGACCCCCGG GAGGCCCTTA GCTCATACGG 901 AATGGACAGC CGACCTCCCA TGGCAATTTT TGAGTTGTTG ATTACATAGT CACGAGCTCT 961 TCGAAAACTG CCCAGTGGGA GTGTCAGTCT GCATTTCAGG ATTTTTGTGA ATAATTGCCT 1021 AATAAAACCC GCAGAGGAGC GAATTGAAGC AACTCATGTC ATGCTTATCA GAGATTCGAT 1081 GCCTGAGGGA A // |
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Primer: MEK-f2 Sequence file: RDB14154_A6Ecc.seq
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>D03441A2_A6Ec_3_MEK-f2_C11_03_ABI08.ab1 1 TCAGAAAGAG TCAAGCCCTC CAACATCCTA GTCAACTCCC GTGGGGAGAT CAAGCTCTGT 61 GACTTTGGGG TCAGCGGGCA GCTCATCGAC GAAATGGCCA ACGAATTCGT GGGCACAAGG 121 TCCTACATGT CGCCAGAAAG ACTCCAGGGG ACTCATTACT CTGTGCAGTC AGACATCTGG 181 AGCATGGGAC TGTCTCTGGT AGAGATGGCG GTTGGGAGGT ATCCCATCCC TCCTCCAGAT 241 GCCAAGGAGC TGGAGCTGAT GTTTGGGTGC CAGGTGGAAG GAGATGCGGC TGAGACCCCA 301 CCCAGGCCAA GGACCCCCGG GAGGCCCCTT AGCTCATACG GAATGGACAG CCGACCTCCC 361 ATGGCAATTT TTGAGTTGTT GGATTACATA GTCAACGAGC CTCCTCCAAA ACTGCCCAGT 421 GGAGTGTTCA GTCTGGAATT TCAAGATTTT GTGAATAAAT GCTTAATAAA AAACCCCGCA 481 GAGAGAGCAG ATTTGAAGCA ACTCATGGTT CATGCTTTTA TCAAGAGATC CGATGCTGAG 541 GAAGTGGATT TTGCAGGTTG GCTCTGCTCC ACCATCGGCC TTAACCAGCC CAGCACACCA 601 ACCCATGCTG CTGGCGTCTA AACTAGTCAG GCATCAAATA AAACGAAAGG CTCAGTCGGA 661 AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC GCTCTCCTGA GTAGGACAAA 721 TCCGCCGGGA GCGGATTTGA ACGTTGCGAA GCAACGGCCC GGAGGGTGGC GGGCAGACGC 781 CCGCCCATAA ACTGCCAGGC ATCAAATTAA GCAGAACGCC ATCCTGACGG ATGGCCTTTT 841 TTGCGTTTCT ACAACTCTTC TGTCGTCATA TCTAACGGCC GACGCGCCTG GCTACGTCTT 901 GCTGGCGTTT CCGCGACGCG AAGCTGAATG CCTTCCCCAT ATGAATTCTT CCTCGCTTCC 961 GGCGGCATCG GAATTGCCCG CCGTGCCAGG CAATTGCTGT TCCAGGCCAG TTAGAATGGA 1021 CGACCATCCA GGAACGCTCA GAATCGCCTC CCGCTTACAG CTAACATTCG GATCCATGGA 1081 GAACGGCGT // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Shindo, Y., Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling. Nat. Commun. 7: 10485 (2016). PMID 26786866. [link to RRC of NBRP] |
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2023.11.23
GNP_filter3_RDBDEP_html_231027.pl