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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-MyosinVa_Full (#RDB14185)

Expression vector of GFP-tagged mouse full-length myosin-Va.

Clone info. Expression vector of GFP-tagged mouse full-length myosin-Va.
Comment Expression was confirmed by the depositor. X57377
Vector backbone pEGFP-C1 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse MyosinVa cDNA
Depositor|Developer Takumi, Toru |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Yoshimura, A. et al., Curr. Biol. 16(23):2345-2351, 2006)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshimura, A. et al., Curr. Biol. 16(23):2345-2351, 2006)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14185 pEGFP-MyosinVa_Full DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-MyosinVa_Full was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14185).

Reference section:

Yoshimura, A., Fujii, R., Watanabe, Y., Okabe, S., Fukui, K., Takumi, T., Myosin-Va facilitates the accumulation of mRNA/protein complex in dendritic spines. Curr. Biol. 16 (23): 2345-2351 (2006). PMID 17141617. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14185_A6F3p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C
Sequence file: RDB14185_A6F3a.seq check
>D03420B8_A6F3_1_EGFP-C_A06_03.ab1
    1 CGCAGGTCTC TCGGCATGGA GAGCTGTACA GTCCGGACTC AGATCTCGAG CTCAAGCTTC
   61 GAATTTTGCG GCCGCAAGGC CTATGGCCGC GTCCGAGCTC TACACCAAGT TTGCCAGGGT
  121 TTGGATCCCT GATCCTGAGG AAGTGTGGAA ATCGGCAGAG TTGCTCAAGG ATTATAAGCC
  181 TGGAGATAAA GTGCTCCTGC TTCACCTCGA GGAAGGGAAG GATTTGGAAT ACCGTCTAGA
  241 CCCAAAGACC GGTGAGCTCC CTCACTTACG GAACCCTGAC ATACTTGTTG GAGAAAATGA
  301 CCTCACAGCC CTCAGCTACC TTCACGAGCC CGCTGTGCTA CATAATCTCC GAGTTCGCTT
  361 CATCGATTCC AAACTTATTT ATACGTATTG TGGAATAGTT CTGGTAGCTA TAAATCCCTA
  421 TGAGCAGCTG CCTATTTATG GAGAAGATAT TATTAATGCC TACAGTGGCC AGAACATGGG
  481 TGACATGGAT CCTCACATCT TCGCAGTAGC TGAAGAGGCT TACAAGCAAA TGGCAAGGGA
  541 TGAACGAAAT CAGTCCATCA TTGTAAGTGG AGAGTCAGGC GCAGGGAAGA CGGTCTCTGC
  601 TAAGTATGCC ATGCGGTACT TCGCAACTGT AAGTGGCTCT GCCAGTGAGG CCAATGTTGA
  661 GGAAAAGGTC TTGGCCTCCA ACCCCATCAT GGAGTCAATT GGAAATGCCA AAACAACCAG
  721 GAATGATAAT AGCAGCCGGT TTGGAAAATA TATTGAAATT GGTTTTGACA AGAGGTACAG
  781 AATCATCGGT GCCAATATGA GAACTTACCT TTTAGAGAAA TCCAGAGTGG TGTTCCAGGC
  841 AGAAGAGGAG AGAAACTACC ATATCTTCTA TCAGCTCTGT GCTTTCGGCA AAGTTACCTG
  901 AGTTTAAAAT GCTGCGGTTA AGAAATGCAG ATAGTTTTCA TTACACGAAG CACGAGTCAG
  961 TCCCTATGAT AGATGAGTTA GATGAATGCC TACGAGATGC GCACACCAGC AGCCTGCACT
1021 CTGCCTGCAT TAGTGAATCG TACGCTGGCA TTATCGAATA CTGCCTGCCA TTCTTCAC
//
Primer: pGL3-RV5F
Sequence file: RDB14185_A6F3b.seq check
>D03420B8_A6F3_1_pGL3_RV5F_E08_14.ab1
    1 ATTCTCAAAT GTGGTATGGC TGATTATGAT CAGTTATCTA GATCCGGTGG ATCCCGGGCC
   61 CGCGGTACCG TCGACTGCAG AATTTTGCGG CCGCAATCGA CCAATTCGGT TTTTTTTTTT
  121 TTTACTTGGC CAGTTACTTT AATGTTGCCA GTTTATTTTT GTGATCCAGT TTGACAATAT
  181 CAACATGATT GGCTCCAAAG CTGCTATGAT ATGTATTCAG TGATTTTCTT CAGTTCTTTG
  241 TGCCTTGTTC ACAACAGTTC ATACATATGG GATTGTATTT TGTATGCCAC TTAAATGCAG
  301 CACTAAAGAT TTAAACTAAA AGCAGTCAGC TGAAGACACA CCCCTTTATC CTTCCTTTCA
  361 CTCCTCCCTG CCACCTTTCA GCTGTATGTA AACTCAGGTT TCCAACTGAT AAAGAATAAA
  421 TGTTGTTTAA AGGTAACAGT ACGAGGGGTG TCCTCCAGGT TGGTCAATCG AAAGGATTCT
  481 GGGGGGAGGG GTGGATTTCC AGTTAACATC TTCCTTTAGA GATAATCAGT ACTTTTTCTT
  541 TAAAAATGCA TTTTCAGTAG CTCACTGGAA ACAACTGTTC ATATTCTGAG GAGAAATGCG
  601 TTTTCTACGT TTGCTTGGAC AGCACTTCAG ACCCGTGCGA TGAAGCCCAG GCCCAGGCTG
  661 GCTGGGATCT GGATGGTTTC TAGGGCTAGG GAGGATGGGT TAAAGGGAAA AGTGACAGGA
  721 AAGATGTGTT TTGCATCCAT GAGCAGCTGT GGAGAGTCTT TCCTGTCTCG TAACCGCATC
  781 TGTATAGTGC GGATAAATGA AACTGAGACC CTTTCTTCAA ACTCATTAAC CGGTGTGTAC
  841 AGATTCAACA CTTTGACAAT CTGGGCCGTG GTCAGGGCGT TGCACATGGA ACAGATGGCT
  901 TCTGCGTCAT CGTCAGTTTT CTTTTTCACT TGCAAAAGCT GAGCGGCCTG GATAAGAGGT
  961 TCCAGAGTCT CCTTGGCCCC ACTGTTCATT AGATTCTTGT CACGTAGCCA TTCTTCCAGT
 1021 TGACTGACAT TGTACCTGAT CTGCATGCCC TTGCTCAGAG CACATGTCCT TGCGCAGAAG
 1081 AAGTTGTTTA GGTGATGGCG CCCCACATGT AGAACATCTG TTTGGACTTA CCTGCCTGAT
1141 AGCTCCGGAT CCAATGCCAA TGCC
//
Primer: SV40pro_F_V2
Sequence file: RDB14185_A6F3c.seq check
>D03420B8_A6F3_1_SV40pro_F_V2_C06_09.ab1
    1 TTTTAGAATT TTTTGGAGGC CTAGGCTTTT GCAAGATCGA TCAAGAGACA GGATGAGGAT
   61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
  601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  781 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCCTTCTAT
  841 CGCCCTTCTT GACGAGTTCT TCTGAGCGGG GACTCTGGGG TTCGAAATGA CCGACCAAGC
  901 GTCCGCCCCA ACCCTGGCCA TCACGAGAAT TCGATTTCCC ACCGCCGGCC TTTCTATGAA
  961 AAGTTGGGCT TCGAAATCGA TTTTCGGGAA CGCCGCTGGA TGATTCTTCA GCGCGGGAAT
1021 TCCATGCTTG AGTCTCGCCT CACTTAGGGA AGACTACTGA ACTCACGAAG GAAG
//
Primer: CMV-Forward
Sequence file: RDB14185_A6F3d.seq check
>D03420B8_A6F3_1_CMV-Forward_D06_12.ab1
    1 CTGTCACGTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGTCGCC ACCATGGTGA
  121 GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG
  181 TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC
  241 TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA
  301 CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG
  361 ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG
  421 ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC
  481 GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG
  541 AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA
  601 AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT
  661 ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA
  721 GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG
  781 AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TCCGGACTCA
  841 GATCTCGAGC TCAAGCTTCG AATTTTGCGG GCCGCAAGGC CTATGGCCGC GTCCGAGCTC
  901 TACACCAAGT TTGCCCAGGG TTTGGGATCC CTGATCCTGA GGAAGTGTGG AAATCCGGCC
  961 AGAGTGCTCA TGAATAATAG CCTGGTAGAT TAAAGTTGCC TTCTTGCATC ACTCGAAGCA
1021 TGCATGATTT GCATACGGTC TAGACCGAAA GAACCAGT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yoshimura, A., Myosin-Va facilitates the accumulation of mRNA/protein complex in dendritic spines. Curr. Biol. 16 (23): 2345-2351 (2006). PMID 17141617. [link to RRC of NBRP]