Please review the QC test results indicated by icon below as well as clone information before placing your order.

pcDNA3.1/Myc-His(+)-HP/C437S

Expression vector of Myc-His tagged human Heparanase (HP) mutant C437S.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Shimizu, S. et al., Cancer Res. 67:7841-7849, 2007)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shimizu, S. et al., Cancer Res. 67:7841-7849, 2007)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14262_A7B2p1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro,T7 pro,insert5' Sequence file: RDB14262_A7B2a.seq
>D03620B7_A6Gj_1_CMV-Forward_E11_05_ABI08.ab1     1 CTTTCCAACT CCCGGCCCCA ATTGACGCAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT    61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA   121 ATACGACTCA CTATAGGGAG ACCCAAGCTG GCTAGTTAAG CTTGGTACCG AGCTCGGATC   181 CACTAGTCCA GTGTGGTGGA ATTCACTAGT GATTATGCTG CTGCGCTCGA AGCCTGCGCT   241 GCCGCCGCCG CTGATGCTGC TGCTCCTGGG GCCGCTGGGT CCCCTCTCCC CTGGCGCCCT   301 GCCCCGACCT GCGCAAGCAC AGGACGTCGT GGACCTGGAC TTCTTCACCC AGGAGCCGCT   361 GCACCTGGTG AGCCCCTCGT TCCTGTCCGT CACCATTGAC GCCAACCTGG CCACGGACCC   421 GCGGTTCCTC ATCCTCCTGG GTTCTCCAAA GCTTCGTACC TTGGCCAGAG GCTTGTCTCC   481 TGCGTACCTG AGGTTCGGTG GCACCAAGAC AGACTTCCTA ATTTTCGATC CCAAGAAGGA   541 ATCAACCTTT GAAGAGAGAA GTTACTGGCA ATCTCAAGTC AACCAGGATA TTTGCAAATA   601 TGGATCCATC CCTCCTGATG TGGAGGAGAA GTTACGGTTG GAATGGCCCT ACCAGGAGCA   661 ATTGCTACTC CGAGAACACT ACCAGAAAAA GTTCAAGAAC AGCACCTACT CAAGAAGCTC   721 TGTAGATGTG CTATACACTT TTGCAAACTG CTCAGGACTG GACTTGATCT TTGGCCTAAA   781 TGCGTTATTA AGAACAGCAG ATTTGCAGTG GAACAGTTCT AATGCTCAGT TGCTCCTGGA   841 CTACTGCTCT TCCAAGGGGT ATAACATTTC TTGGGAAACT AGGCAATGAA CCTAACAGTT   901 TCCTTAAGAA GGCTGATATT TTCATCAATG GGTCGCAGTT AGGGAGAAGA TTTTATTCAA   961 TTTGCATAAA CTTCTAAGAA AGGTCCACCC TTTCAAAAAT GCAAAACTCT TATGGTTCCC  1021 TGATGTTGGT CAGCCTCGAA GAAAGGACGG GCTAAGATGG CTGAAGAGCT TCCCTGAACG 1081 CCTGGTGAGA GTTGATTGAA TCAGTACATG GTCATTCAAC T //
Primer: BGH_rev2 Region: bGHp(A),6xHis,Myc,insert3' Sequence file: RDB14262_A7B2b.seq
>D03620B7_A6Gj_1_BGH_rev2_F11_06_ABI08.ab1     1 CATCAAGGGT AGGAGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC    61 AGTCGAGGCT GATCAGCGGG TTTAAACTCA ATGGTGATGG TGATGATGAC CGGTATGCAT   121 ATTCAGATCC TCTTCTGAGA TGAGTTTTTG TTCGAATGGG TGACCTCGAG CGGCCGCCAC   181 TGTGCTGGAT ATCTGCAGAA TTCGATTGAT GCAAGCAGCA ACTTTGGCAT TTCTTATCAC   241 AAAAAAACTA TATGAGAAAG CTGGCAAGCC CAGTGAACTT CCTGGCCGGA GAGGTTTTTC   301 CATTAAAGGT GGCAAGGTTT GATCATCCAC CATCTTTAGA GTTAGACCAT TGAGTTGGAC   361 AGATTTGGAA AGTAATCCAT GAGGTCCCAA AGGTCTTAGA AGGTATTTAT CCACTTGCTT   421 GTTAGAAAAA GGATAGGGTA ACCGCAAGTA CTTGGTGACA TTATGGAGGT TTATGGCATA   481 CAGAGTTAAA TCTCCTTCTT TATACCTTGG ATTGTCAGTG TTTGTGCTAT GAAGGTATAC   541 TCGAAGCTTC CTTCTCTTTG AACCTTGCAC GCTTGCCATT AACACCTTGG TGCCCACCAA   601 TTTCTTGAAC AGAAGAGATA GCCAATAATC AGGTAAAGGA TCGAAGTTTT CATCCACTAA   661 ATGGTAGTTT CCTGCTCCAA AGAATACTTG CCTCATCACC ACTTCTATTC CCATTCGGGC   721 TGACAGGCCC AATTTATCCA GCCACATAAA GCCAGCTGCA AAGGTGTCGG ATAGCAAGGG   781 CGCTCCGCCT CCATATGCAG AGCTTGTCTC TCCTAACCAG ACCTTCTTGC CAGGCCTGGT   841 GCTCTCAACC ACCTGGAAAA CTTTTTGCAC AGATGAAATA AAAATGTCCA ATACATCAGG   901 GTTTAGAAAA TCTTCCCTGG TAGCAGTCCG TCCATTCAAA TAGTAGTGAT GCCATGTAAC   961 TGAATCAATC ACTTCTCCAC CAGCCTTCAG GAAGCTCTCA GCATCTTAGC CGTCTTTTCT  1021 TCGAGCTTGA ACGACAATCA GACCCATAGA GGTTTTGGCA ATTTTGGAGT GGACATCTAG 1081 AGTATGCATT GGATAAATCT TTCTCCTACC TTGGC //
Primer: RDB14252#1 Region: insert mid Sequence file: RDB14262_A7B2c.seq
>D03620B7_A6Gj_1_RDB14252#1_C11_08_ABI24.ab1     1 TCTGGAATGG ATGATCTTTG GCCTAATGCG TTATTAAGAA CAGCAGATTT GCAGTGGAAC    61 AGTTCTAATG CTCAGTTGCT CCTGGACTAC TGCTCTTCCA AGGGGTATAA CATTTCTTGG   121 GAACTAGGCA ATGAACCTAA CAGTTTCCTT AAGAAGGCTG ATATTTTCAT CAATGGGTCG   181 CAGTTAGGAG AAGATTTTAT TCAATTGCAT AAACTTCTAA GAAAGTCCAC CTTCAAAAAT   241 GCAAAACTCT ATGGTCCTGA TGTTGGTCAG CCTCGAAGAA AGACGGCTAA GATGCTGAAG   301 AGCTTCCTGA AGGCTGGTGG AGAAGTGATT GATTCAGTTA CATGGCATCA CTACTATTTG   361 AATGGACGGA CTGCTACCAG GGAAGATTTT CTAAACCCTG ATGTATTGGA CATTTTTATT   421 TCATCTGTGC AAAAAGTTTT CCAGGTGGTT GAGAGCACCA GGCCTGGCAA GAAGGTCTGG   481 TTAGGAGAGA CAAGCTCTGC ATATGGAGGC GGAGCGCCCT TGCTATCCGA CACCTTTGCA   541 GCTGGCTTTA TGTGGCTGGA TAAATTGGGC CTGTCAGCCC GAATGGGAAT AGAAGTGGTG   601 ATGAGGCAAG TATTCTTTGG AGCAGGAAAC TACCATTTAG TGGATGAAAA CTTCGATCCT   661 TTACCTGATT ATTGGCTATC TCTTCTGTTC AAGAAATTGG TGGGCACCAA GGTGTTAATG   721 GCAAGCGTGC AAGGTTCAAA GAGAAGGAAG CTTCGAGTAT ACCTTCATAG CACAAACACT   781 GACAATCCAA GGTATAAAGA AGGAGATTTA ACTCTGTATG CCATAAACCT CCATAATGTC   841 ACCAAGTACT TGCGGTTACC CTATCCTTTT TCTAACAAGC AAGTGGATAA ATACCTTCTA   901 AGACCTTTGG GACCTCATGG ATTACTTTCC AAATCTGTCC AACTCAATGG TCTAACTCTA   961 AAGATGGTGG ATGATCAAAC CCTTGCCACC TTTAATGGAA AAACCCTCTC CGGGCCAGGA  1021 GTTCACTGGG CTTTGCCCAG CTTTCTCATA TAGTTTTTTT TGTGATAAGA AATGCCAAAG  1081 AGCTGCTTGC ATCATCGGAA TCTGCAGATA TCAAGCACAG TTGACCACGC TCGGAGGCTC 1141 CACCCCCAAT TTCCGAAC //
Primer: SV40pro_F Region: SV40 pro_ori,NeoR Sequence file: RDB14262_A7B2d.seq
>D03620B7_A6Gj_1_SV40pro_F_C08_08_ABI24.ab1     1 CCCCTGGTCC TGGGGCATTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC    61 AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA   121 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG   181 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC   241 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG   301 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC   361 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG   421 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT   481 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG   541 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT   601 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC   661 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG   721 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC   781 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT   841 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT   901 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGCGAAATGA CCGACCAAGC   961 GACGCCCAAC CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTTCTA TGAAGGTTGG  1021 GCTTCGGATC GTTTTCCGGA CGCCGGGCTG GATGATCTCA GCGCGGGGGA TCTCATGCCT  1081 GAGTCTCGCC CACCCCCACT GTTATGGCAG CTATATGTTA CAATTAAAGG CATTAGCAAT 1141 CCACAAATTT CACA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl