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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-HA-RACK1

Expression vector of HA-tagged mouse receptor of activated protein kinase C1 (RACK1) full-length (1-317 aa).

Catalog number RDB14335
Resource name pEF-HA-RACK1
Clone info. Expression vector driven by the EF1 promoter for HA-tagged mouse receptor of activated protein kinase C1 (RACK1) full-length (1-317 aa), see Fig. 1b of Marubashi, S. et al. J. Invest. Dermatol., 136 (8): 1672-1680, 2016.
Comment Expression was confirmed by the depositor with immunoprecipitation.
Vector backbone pEF-HA (Plasmid)
Selectable markers Amp^r
Gene/insert name mouse RACK1 cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank
External Database
mouse RACK1
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Marubashi, S. et al., J. Invest. Dermatol., 136 (8): 1672-1680, 2016)
          Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Marubashi, S. et al., J. Invest. Dermatol., 136 (8): 1672-1680, 2016)。
          付加的提供条件 pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14335 pEF-HA-RACK1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Marubashi, S., A Varp-Binding Protein, RACK1, Regulates Dendrite Outgrowth through Stabilization of Varp Protein in Mouse Melanocytes. J. Invest. Dermatol. 136 (8): 1672-1680 (2016). PMID 27066885.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14335_A6I8p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Sequence file: RDB14335_A6I8a.seq check
          >D03786A3_A6I8_1_Efla-C_F_E07_13_ABI24.ab1
              1 TCGTGAGTGC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA
             61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGCGGCC GCGGGAATTC
            121 GATTCCACCA TGGGTTACCC ATACGACGTC CCAGACTACG CTGGATCCAT GACCGAGCAG
            181 ATGACCCTTC GCGGGACCCT TAAGGGCCAC AATGGATGGG TAACACAGAT CGCCACCACA
            241 CCGCAGTTCC CGGACATGAT CCTGTCTGCG TCTCGAGACA AGACCATCAT CATGTGGAAG
            301 CTGACCAGAG ATGAGACCAA CTATGGCATA CCACAGCGTG CTCTGAGAGG TCACTCCCAC
            361 TTCGTTAGTG ATGTTGTTAT CTCCTCTGAT GGTCAGTTTG CCCTCTCGGG CTCCTGGGAC
            421 GGAACGCTGC GCCTCTGGGA TCTCACAACG GGCACCACCA CAAGGCGATT TGTCGGCCAC
            481 ACCAAGGATG TGTTGAGCGT GGCCTTCTCC TCTGACAACC GGCAGATTGT CTCTGGGTCC
            541 CGAGACAAGA CCATAAAGTT ATGGAATACT CTGGGTGTCT GCAAGTACAC GGTCCAGGAT
            601 GAGAGTCATT CAGAATGGGT GTCTTGTGTC CGCTTCTCCC CGAACAGCAG CAACCCTATC
            661 ATCGTCTCCT GCGGATGGGA CAAGCTGGTC AAGGTGTGGA ATCTGGCTAA CTGCAAGCTA
            721 AAGACCAACC ACATTGGCCA CACTGGCTAC CTGAACACAG TGACTGTCTC TCCAGATGGA
            781 TCCCTCTGTG CTTCTGGAGG CAAGGATGGC CAGGCTATGC TGTGGGATCT CAATGAAGGC
            841 AAGCACCTCT ACACTTTAGA TGGTGGGGAC ATCATCAATG CCTTGTGCTT CAGCCCCAAC
            901 CGCTACTGGC TCTGCGCTGC CACTGGCCCC AGCATCAAGA TCTGGGACTT GGGAGGGCAA
            961 GATCATTGTA GATGAATTGA AGCAGAAGTT ATCAGCAACA GCAGCAGCAG AGCCACCCCC
           1021 AGTGTACTCT TGGCATGGTC TGCTGAATGC AGACTCTGGT TTGCCTTGCT ATACAGACAG
          1081 ACTTCGATGC GAGCTATGCC AAGGCTTACC TAATTGGTA
          //
          Primer: hGCSF-R
          Sequence file: RDB14335_A6I8b.seq check
          >D03786A3_A6I8_1_hGCSF_R_F07_16_ABI24.ab1
              1 CTGGGGACTG ATCTCTCAGG GTCCCAGGTG GGGACCCTCA CTCTAGAGCG GCCGCTATGG
             61 TCGACCTGCA GGCGGCCGCG AATTCACTAG TGATTTTAGC GGGTACCAAT AGTTACCTGC
            121 CATACTCGCA CCAAGTTGTC TGTATAGCCA GCAAACAGAG TCTGGCCATC AGCAGACCAT
            181 GCCAAAGAGG TACACTGGGG TGGCTCTGCC TTGCTGCTGG TGCTGATAAC TTCTTGCTTC
            241 AATTCATCTA CAATGATCTT GCCCTCCAAG TCCCAGATCT TGATGCTGGG GCCAGTGGCA
            301 GCGCAGAGCC AGTAGCGGTT GGGGCTGAAG CACAAGGCAT TGATGATGTC CCCACCATCT
            361 AAAGTGTAGA GGTGCTTGCC TTCATTGAGA TCCCACAGCA TAGCCTGGCC ATCCTTGCCT
            421 CCAGAAGCAC AGAGGGATCC ATCTGGAGAG ACAGTCACTG TGTTCAGGTA GCCAGTGTGG
            481 CCAATGTGGT TGGTCTTTAG CTTGCAGTTA GCCAGATTCC ACACCTTGAC CAGCTTGTCC
            541 CATCCGCAGG AGACGATGAT AGGGTTGCTG CTGTTCGGGG AGAAGCGGAC ACAAGACACC
            601 CATTCTGAAT GACTCTCATC CTGGACCGTG TACTTGCAGA CACCCAGAGT ATTCCATAAC
            661 TTTATGGTCT TGTCTCGGGA CCCAGAGACA ATCTGCCGGT TGTCAGAGGA GAAGGCCACG
            721 CTCAACACAT CCTTGGTGTG GCCGACAAAT CGCCTTGTGG TGGTGCCCGT TGTGAGATCC
            781 CAGAGGCGCA GCGTTCCGTC CCAGGAGCCC GAGAGGGCAA ACTGACCATC AGAGGAGATA
            841 ACAACATCAC TAACGAAGTG GGAGTGACCT CTCAGAGCAC GCTGTGGTAT GCCATAGTTG
            901 GTCTCATCTC TGGTCAGCTT CCACATGATG ATGGTCTTGT CTCGAGACGC AGACAGGATC
            961 ATGTCCGGGA ACTGCGGGTG TGGGTGGCGA TCTGTGTTAC CCATCCATTT GTGGGCCCTT
           1021 TAGGGTCCCG CGAAGGGTCA TCTGCTCGGT CATGGGAATC AGCGTTAGTC TGGGGACGTC
           1081 GTAATGGTAC TCATGATTGG TAATTTCGGA CTTCCCCCGC CGGATCGCCC TCTCTTAGAG
          1141 A
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Marubashi, S., A Varp-Binding Protein, RACK1, Regulates Dendrite Outgrowth through Stabilization of Varp Protein in Mouse Melanocytes. J. Invest. Dermatol. 136 (8): 1672-1680 (2016). PMID 27066885.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl